Study on the Chemical Composition and Anti Cervical Cancer Cell Activity of White Spirit Mushroom
White Spirit Mushroom, also known as Tian Shan White Lingzhi, Awei Mushroom, Ferula Mushroom, etc., is a higher fungus belonging to the Pleurotus genus of the Pleurotaceae order in the Basidiomycota phylum. Its Chinese scientific name is Pleurotus tuoliensis. Wild parasitic or saprophytic growth occurs in the enlarged rhizomes of the traditional Chinese medicine Ferula. White spirit mushrooms are as white as silk in color and have a delicious taste. They are known as the “Queen of Edible Fungi” among the edible mushroom family. There are reports that the nutritional composition of white spirit mushrooms is very comprehensive, containing various amino acids, fats, vitamins, and small molecules. At present, white spirit mushrooms are widely planted in Xinjiang and have significant economic value. Modern pharmacological studies have shown that extracts of white mushrooms have various pharmacological effects such as anti-tumor, immune enhancement, and radiation resistance. Related chemical studies have shown that polysaccharides from Pleurotus ostreatus are the main active ingredients and have been extensively studied. In order to further study the material basis of White Spirit Mushroom, this article isolated and identified its small molecule chemical components, and detected the anti cervical cancer cell activity of the components, providing reference for further development of the value of White Spirit Mushroom.

This study explored the chemical composition of Pleurotus ostreatus and obtained 11 compounds, including 6 terpenes (1, 5, 7, 8, 9, 11), 4 polyketones (2, 3, 4, 6), and 1 other compound (10), all of which were isolated from Pleurotus ostreatus for the first time. The screening results of anti-tumor activity showed that compounds 1-4, 6-8, and 11 all exhibited strong anti-tumor activity. Further analysis of the structure-activity relationship of the separated compounds reveals that compounds with a 6/6/6 ring system structure and different connecting groups at the C-3 position may have certain effects on anti-tumor activity. At present, there are few reports on the small molecule compounds present in White Spirit Mushroom both domestically and internationally. This study is the first to investigate the chemical composition of White Spirit Mushroom, which further enriches the chemical composition of White Spirit Mushroom and lays the foundation for further understanding of its active substances. The discovered compounds with significant anti-tumor activity can be extensively enriched in White Spirit Mushroom for further research on modification and modification, thereby expanding the application scope of White Spirit Mushroom and promoting local economic development.