Study on the Amino Alkaloids in Sea Breeze Vine
Sea Breeze Vine is a dried vine stem of Piper kadsura (Choisy) Ohwi, a pepper plant in the Piperaceae family. It is mainly produced in Taiwan, Yunnan, Guangdong, and other regions of China. Haifengteng has a pungent and bitter taste, a slightly warm nature, and belongs to the liver meridian. It has the effects of “dispelling wind dampness, unblocking meridians, and relieving rheumatism and pain”, and is clinically used to treat wind cold dampness rheumatism, limb pain, and other symptoms. Pharmacological studies have shown that sea cucumber vine has significant medicinal value in the treatment of Alzheimer’s disease, anti-inflammatory, analgesic, and organ ischemia-reperfusion. Chemical composition studies have found that sea breeze vine mainly contains components such as lignin, alkaloids, flavonoids, and volatile oils. Among them, amide alkaloids are characteristic components of this genus of plants and have a relatively high content, which has also been reported in sea breeze vine. At present, scholars at home and abroad mainly focus on the new lignans and their applications in the treatment and prevention of Alzheimer’s disease, ischemic cerebrovascular disease, etc. in the sea breeze vine, but there are few reports on the amide alkaloids in this plant. Parkinson’s disease (PD) is the second most common neurodegenerative disease after Alzheimer’s disease. In recent years, studies have found that some traditional Chinese medicine formulas or preparations containing sea breeze vine have good improvement effects on PD, but there is a lack of corresponding chemical composition research basis. Based on the above issues, we conducted research on amide alkaloids in sea cucumber vine and constructed a PD cell model using image-induced damage to PC12 cells. The isolated compounds were screened for neuroprotective activity in vitro, providing a reference for the development and utilization of active ingredients in this plant in the future.

In this experiment, a total of 13 amide alkaloids were isolated from the dichloromethane fraction of sea cucumber vine. Among them, compound 1 is a new compound, compound 2 is a new natural product, and compounds 3-7, 9, and 10 were isolated from this plant for the first time. Pharmacological experimental studies have shown that compound 6 has a significant protective effect on photo induced PC12 cell damage at 20 μ M (P<0. 05) 01) suggests that it has potential therapeutic reference value for Parkinson’s disease, and also has certain reference value for the subsequent study of chemical composition and biological activity of this genus of plants. This study not only further enriches the structural diversity of amide alkaloids in sea cucumber vine, but also has certain reference value for the research of amide alkaloids in the treatment and prevention of neurodegenerative diseases in sea cucumber vine.