{"id":8989,"date":"2024-08-15T15:47:29","date_gmt":"2024-08-15T15:47:29","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=8989"},"modified":"2024-08-15T15:47:29","modified_gmt":"2024-08-15T15:47:29","slug":"bn7-in-boju","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/tr\/bn7-bojuda\/","title":{"rendered":"Boju'da endofitik bakteri BN7'nin tan\u0131mlanmas\u0131 ve biyokontrol ve b\u00fcy\u00fcmeyi te\u015fvik edici i\u015flevleri \u00fczerine \u00e7al\u0131\u015fma"},"content":{"rendered":"<p>Boju'da endofitik bakteri BN7'nin tan\u0131mlanmas\u0131 ve biyokontrol ve b\u00fcy\u00fcmeyi te\u015fvik edici i\u015flevleri \u00fczerine \u00e7al\u0131\u015fma<br \/>\nBitki endofitik bakterileri, ya\u015fam \u00f6yk\u00fclerinin belirli bir a\u015famas\u0131nda veya t\u00fcm a\u015famas\u0131nda sa\u011fl\u0131kl\u0131 bitki dokular\u0131nda veya h\u00fccrelerinde ya\u015fayan ve konak\u00e7\u0131da belirgin hastal\u0131k semptomlar\u0131na neden olmayan bir grup mikroorganizmay\u0131 ifade eder. Bitkilerin t\u00fcm b\u00fcy\u00fcme s\u00fcreci, konuk\u00e7u bitki ve endofitik toplulu\u011fun ya\u015fam s\u00fcrecidir. Endofitler, konuk\u00e7u bitkiler ve onlar\u0131 \u00e7evreleyen ya\u015fam ortamlar\u0131 ile karma\u015f\u0131k ekolojik ili\u015fkiler kurar. Endofitik bakteriler, konuk\u00e7ununkine benzer metabolitler \u00fcretebilir ve bitkilere biyotik ve abiyotik streslere direnme yetene\u011fi kazand\u0131rabilir. Endofitik bakteriler ve bitkiler aras\u0131ndaki uzun s\u00fcreli etkile\u015fim s\u0131ras\u0131nda, endofitik bakteriler konuk\u00e7u bitkinin ayr\u0131\u015fmas\u0131n\u0131 ve fosfor ve potasyum gibi mineral elementlerin emilimini art\u0131rma \u00f6zelli\u011fi g\u00f6sterir. Bitki patojenleri \u00fczerinde antagonistik etkilerin yan\u0131 s\u0131ra fosfor, potasyumu hidrolize etme ve uzama \u00fcretme yetene\u011fine sahip \u00e7ok i\u015flevli bakteri t\u00fcrlerinin taranmas\u0131, tar\u0131mda uygulama i\u00e7in b\u00fcy\u00fck bir potansiyele sahiptir.<\/p>\n<p>Krizantemlerin \u0131s\u0131y\u0131 temizleme, \u0131s\u0131y\u0131 hafifletme ve antioksidasyonun yan\u0131 s\u0131ra antibakteriyel, anti-enflamatuar, kilo verme ve y\u00fcksek \u00fcrik asitli kanla ilgili hastal\u0131klar\u0131 hafifletme etkileri vard\u0131r. D\u00f6rt b\u00fcy\u00fck t\u0131bbi krizantemden biri olarak, ara\u015ft\u0131rma grubumuz endofitik mantarlar\u0131 \u00fczerinde \u00f6n \u00e7al\u0131\u015fmalar y\u00fcr\u00fctm\u00fc\u015ft\u00fcr. Bu deney, Chrysanthemum morifolium'daki endofitik bakteri BN7'nin antibakteriyel, fosfat \u00e7\u00f6z\u00fcnd\u00fcr\u00fcc\u00fc, potasyum \u00e7\u00f6z\u00fcnd\u00fcr\u00fcc\u00fc, b\u00fcy\u00fcmeyi te\u015fvik edici, sel\u00fcloz par\u00e7alay\u0131c\u0131 ve antioksidan aktivitelerini incelemi\u015f ve biyolojik kontrol kaynaklar\u0131n\u0131 zenginle\u015ftirmek ve biyo g\u00fcbreler geli\u015ftirmek i\u00e7in temel olu\u015fturarak \u00f6n tan\u0131mlamas\u0131n\u0131 yapm\u0131\u015ft\u0131r.<\/p>\n<p>1970'lerin sonunda, Dobereiner ve arkada\u015flar\u0131 azot sabitleyici salyangozlar\u0131n baklagil olmayan bitkilerin b\u00fcy\u00fcmesini te\u015fvik edebilece\u011fini ke\u015ffetti; 1980'lerin sonunda, Kanadal\u0131 bir \u015firket olan Philom Bios, bu\u011fday, kolza tohumu ve bezelye gibi \u00fcr\u00fcnler i\u00e7in \u00fcr\u00fcn verimini 10% ila 15% art\u0131rabilen fosfat \u00e7\u00f6z\u00fcnd\u00fcr\u00fcc\u00fc bakteriler, azot sabitleyici bakteriler ve kar\u0131\u015f\u0131k fosfat ve azot sabitleyici bakteriler \u00fcretmeye ve satmaya ba\u015flad\u0131. Daha sonra, bitkilerdeki endofitik bakteriler hem yurt i\u00e7inde hem de yurt d\u0131\u015f\u0131nda giderek s\u0131cak bir ara\u015ft\u0131rma konusu haline geldi. 2009 y\u0131l\u0131nda Cheng ve arkada\u015flar\u0131 Huazhong Building'den PCE45 adl\u0131 bir endofitik bakteriyi taram\u0131\u015f ve bu bakterinin m\u0131s\u0131r k\u0131v\u0131rc\u0131kl\u0131\u011f\u0131 ve pirin\u00e7 patlamas\u0131 mantar\u0131 (Pyricularia oryzae) Cav gibi \u00e7e\u015fitli patojenler \u00fczerinde inhibit\u00f6r etkileri olan PCP-1 adl\u0131 bir antibakteriyel peptit \u00fcretti\u011fini tespit etmi\u015ftir. 2010 y\u0131l\u0131nda Bressan ve arkada\u015flar\u0131 m\u0131s\u0131r bitki dokular\u0131ndan kitinaz \u00fcretebilen alt\u0131 endofitik Bacillus t\u00fcr\u00fc izole etmi\u015f ve bunlar\u0131n hepsi Fusarium graminearum \u00fczerinde inhibit\u00f6r etki g\u00f6stermi\u015ftir. 2015 y\u0131l\u0131nda, Zhao ve arkada\u015flar\u0131 soya fasulyesi nod\u00fcllerinden 36 fosfor \u00e7\u00f6z\u00fcnd\u00fcr\u00fcc\u00fc endofitik bakteri taram\u0131\u015f, bunlar aras\u0131nda DD291 su\u015funun etanol \u00e7\u00f6zeltisinde 452 mg\/L'ye kadar \u00e7\u00f6z\u00fcn\u00fcr fosfor i\u00e7eri\u011fine sahip oldu\u011fu ve baz\u0131 fosfor \u00e7\u00f6z\u00fcnd\u00fcr\u00fcc\u00fc su\u015flar\u0131n soya fasulyesi b\u00fcy\u00fcmesi \u00fczerinde te\u015fvik edici bir etkiye sahip oldu\u011fu g\u00f6r\u00fclm\u00fc\u015ft\u00fcr. 2015 y\u0131l\u0131nda Kang ve arkada\u015flar\u0131, yoncadaki endofitik bakteriler ASR16 ve ALR33'\u00fcn fosfor \u00e7\u00f6z\u00fcnd\u00fcrme aktivitesi ile bitki b\u00fcy\u00fcme hormonu ve siderofor \u00fcretebildi\u011fini ve yonca b\u00fcy\u00fcmesi \u00fczerinde \u00f6nemli bir te\u015fvik edici etkiye sahip oldu\u011funu bulmu\u015flard\u0131r. 2018'de Gao ve arkada\u015flar\u0131 Niwala'daki yabani pirin\u00e7ten 57 endofitik bakteri izole etmi\u015f, bunlardan 44 endofitik azot sabitleyici bakterinin lignin \u00fcretme kabiliyeti oldu\u011funu ve 25 su\u015fun \u00e7e\u015fitli derecelerde potasyum \u00e7\u00f6z\u00fcnd\u00fcrme kabiliyetine sahip oldu\u011funu belirtmi\u015ftir. Bai ve arkada\u015flar\u0131 2018 y\u0131l\u0131nda yabani Polygonatum sibiricum k\u00f6klerinden Salmonella typhimurium, Pseudomonas aeruginosa ve Bacillus thuringiensis'e kar\u015f\u0131 \u00f6nemli antibakteriyel aktivite sergileyen endofitik bir Bacillus HJ-1 izole etmi\u015ftir.<\/p>\n<p>\u015eu anda, Chrysanthemum morifolium'un endofitik bakterileri \u00fczerine nispeten az ara\u015ft\u0131rma vard\u0131r ve Chrysanthemum morifolium'un endofitik bakterileri taraf\u0131ndan b\u00fcy\u00fcmenin te\u015fvik edilmesine ili\u015fkin \u00e7ok az rapor bulunmaktad\u0131r. Bu \u00e7al\u0131\u015fmada, Chrysanthemum morifolium'un endofitik bakterisi BN7, bu\u011fday Fusarium graminearum FM1'e kar\u015f\u0131 68%'lik en y\u00fcksek inhibisyon oran\u0131 ile m\u0131s\u0131r e\u011fri top mantar\u0131 CY1 dahil olmak \u00fczere alt\u0131 bitki patojenine kar\u015f\u0131 antagonistik etkiler sergilemi\u015ftir. Bu su\u015f ayr\u0131ca fosfor, potasyumu hidrolize etme ve s\u0131ras\u0131yla 108.38, 32.03 ve 164.39 mg\/L konsantrasyonlarda IAA \u00fcretme yeteneklerine sahiptir. BN7 su\u015funun inorganik fosforu hidrolize etme yetene\u011fi (68,71 mg\/L), kafur a\u011fa\u00e7lar\u0131ndaki endofitik Bacillus subtilis ZS-3'\u00fcn (20,16 mg\/L) yakla\u015f\u0131k \u00fc\u00e7 kat\u0131d\u0131r ve bu, ikincisinin daha k\u0131sa yeti\u015ftirme s\u00fcresiyle (24 saat) ilgili olabilir; Ayr\u0131ca, BN7 su\u015funun uzun hormon \u00fcretme yetene\u011fi, Gao Chen ve arkada\u015flar\u0131 taraf\u0131ndan uzun hormon \u00fcretme yetene\u011fi en g\u00fc\u00e7l\u00fc olan su\u015f olarak se\u00e7ilen N8 su\u015funun (41,66 mg\/L) d\u00f6rt kat\u0131d\u0131r. BN7 su\u015fu, \u00fc\u00e7\u00fcnc\u00fc g\u00fcnde s\u0131ras\u0131yla 168,78U\/mL ve 79,87U\/mL CMCase ve FPase enzim aktiviteleri ile sel\u00fclozu par\u00e7alama yetene\u011fine de sahiptir. Ayr\u0131ca, daha \u00f6nce taranan endofitik mantar BJF10'un (67.7%) DPPH s\u00fcp\u00fcrme oran\u0131ndan \u00e7ok daha y\u00fcksek olan 82.13%'ye kadar DPPH serbest radikal s\u00fcp\u00fcrme aktivitesine sahiptir. Morfolojik \u00f6zellikler, fizyolojik ve biyokimyasal deneyler ve 16S rDNA dizisinin analizine dayanarak, BN7 su\u015fu Bacillus subtilis olarak tan\u0131mlanm\u0131\u015ft\u0131r. Bu nedenle, Bacillus subtilis BN7, bitki patojenlerini antagonize etme, fosfor ve potasyumu hidrolize etme ve IAA \u00fcretme yetene\u011fine sahip endofitik bir bakteridir ve biyokontrol ve b\u00fcy\u00fcmeyi te\u015fvik edici bir ajan olarak geli\u015ftirilme potansiyeline sahiptir.<\/p>","protected":false},"excerpt":{"rendered":"<p>Identification of endophytic bacterium BN7 in Boju and study on its biocontrol and growth promoting functions Plant endophytic bacteria refer to a group of microorganisms that live in healthy plant tissues or cells during a certain stage or the entire stage of their life history and do not cause obvious disease symptoms in the host. 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