{"id":8737,"date":"2024-08-14T19:43:48","date_gmt":"2024-08-14T19:43:48","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=8737"},"modified":"2024-08-14T19:43:48","modified_gmt":"2024-08-14T19:43:48","slug":"sargentol-in-red-vine-on-pc12-cells","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/tr\/pc12-hucreleri-uzerinde-kirmizi-asma-icinde-sargentol\/","title":{"rendered":"K\u0131rm\u0131z\u0131 asmadaki sargentol\u00fcn PC12 h\u00fccreleri \u00fczerindeki n\u00f6roprotektif etkisi ve molek\u00fcler kenetlenme \u00e7al\u0131\u015fmas\u0131"},"content":{"rendered":"

K\u0131rm\u0131z\u0131 asmadaki sargentol\u00fcn PC12 h\u00fccreleri \u00fczerindeki n\u00f6roprotektif etkisi ve molek\u00fcler kenetlenme \u00e7al\u0131\u015fmas\u0131
\nN\u00f6ronlar\u0131n hasar\u0131 ve \u00f6l\u00fcm\u00fc, temel olarak oksidatif stres, n\u00f6roinflamasyon, eksitotoksisite, kalsiyum iyonu (Ca2+) a\u015f\u0131r\u0131 y\u00fcklenmesi, mitokondriyal hasar, apoptotik proteinler, otofaji vb. dahil olmak \u00fczere \u00e7ok \u00e7e\u015fitli s\u00fcre\u00e7leri i\u00e7erir. N\u00f6roproteksiyon, n\u00f6rolojik hastal\u0131klar\u0131n \u00f6nlenmesi ve tedavisinde \u00f6nemli bir ba\u011flant\u0131d\u0131r. Kurkumin, resveratrol ve ginsenosidler gibi do\u011fal \u00fcr\u00fcnler, ROS'u d\u00fczenleyerek, anti-enflamatuar etkileri ve eksitotoksisiteyi inhibe ederek iyi n\u00f6roprotektif etkiler g\u00f6stermi\u015ftir. \u0130skemik beyin hasar\u0131 ile m\u00fccadelede ve \u00f6\u011frenme ve bili\u015fsel yeteneklerin geli\u015ftirilmesinde umut verici uygulamalara sahiptirler.
\nSargentodoxa cuneta (Oliv.) Rehd Et Wils. detoksifikasyon, karbonk\u00fclleri giderme, kan dola\u015f\u0131m\u0131n\u0131 te\u015fvik etme, a\u011fr\u0131y\u0131 hafifletme ve r\u00fczgar ve rutubeti giderme etkilerine sahip \u00c7in'e \u00f6zg\u00fc bir bitkidir. K\u0131rm\u0131z\u0131 asmadaki kimyasal bile\u015fenler temel olarak fenolik asitler, fenilpropanoidler, lignanlar vb. i\u00e7erir. Ekstraktlar\u0131 anti-enflamatuar, antioksidan ve n\u00f6roprotektif etkiler gibi farmakolojik aktivitelere sahiptir. \u00d6n ara\u015ft\u0131rmada, laboratuvar\u0131m\u0131z yabani k\u0131rm\u0131z\u0131 asmalardan fenilpropanoid glikozit bile\u015fi\u011fi sargentol\u00fc (6 kg bitki asma sap\u0131ndan 190 mg izole edilmi\u015ftir) izole etmi\u015ftir, ancak bu bile\u015fi\u011fin farmakolojik aktivitesi hakk\u0131nda \u00e7ok az rapor vard\u0131r. Bu makale, sargentol\u00fcn PC12 h\u00fccre hasar\u0131 modeli \u00fczerindeki koruyucu etkisini de\u011ferlendirmeyi ve geleneksel \u00c7in t\u0131bb\u0131 kaynaklar\u0131ndan n\u00f6roprotektif aktif bile\u015fenlerin ke\u015ffi i\u00e7in bilimsel temel sa\u011flamak amac\u0131yla molek\u00fcler yerle\u015ftirme teknolojisine dayal\u0131 olas\u0131 etki mekanizmas\u0131n\u0131 tart\u0131\u015fmay\u0131 ama\u00e7lamaktad\u0131r.<\/p>\n

PC12 h\u00fccrelerinde bal\u0131k asma ketonu kaynakl\u0131 hasar genellikle Parkinson hastal\u0131\u011f\u0131n\u0131n bir h\u00fccre modelini olu\u015fturmak i\u00e7in kullan\u0131l\u0131rken, PC12 h\u00fccrelerinde NaN3 kaynakl\u0131 hasar genellikle anti Alzheimer hastal\u0131\u011f\u0131 bile\u015fiklerinin \u00f6n taramas\u0131 i\u00e7in kullan\u0131l\u0131r. Bu \u00e7al\u0131\u015fmada, sargentol, fisetin ve NaN3 taraf\u0131ndan ind\u00fcklenen PC12 h\u00fccre hasar\u0131 \u00fczerinde \u00f6nemli bir inhibisyon g\u00f6stermedi (P>0.05), ancak H2O2 hasar modeline en duyarl\u0131 olan\u0131yd\u0131 ve PC12 h\u00fccrelerinde H2O2 taraf\u0131ndan ind\u00fcklenen h\u00fccre i\u00e7i ROS \u00fczerinde \u00f6nemli bir inhibit\u00f6r etkiye sahipti.
\nSargentol\u00fcn n\u00f6roprotektif mekanizmas\u0131n\u0131 daha fazla ara\u015ft\u0131rmak i\u00e7in, bu \u00e7al\u0131\u015fmada ba\u011flay\u0131c\u0131 hedef proteinlerini sanal olarak taramak i\u00e7in AutoDock Vina yaz\u0131l\u0131m\u0131 kullan\u0131lm\u0131\u015ft\u0131r. H2O2 hasarl\u0131 h\u00fccre modelinde ROS etkisinin h\u00fccre hasar\u0131 veya apoptozun yayg\u0131n bir nedeni oldu\u011fu g\u00f6z \u00f6n\u00fcnde bulundurularak, molek\u00fcler yerle\u015ftirme i\u00e7in esas olarak ROS etkisi ve inflamasyonla ilgili proteinler olmak \u00fczere 14 protein se\u00e7tik.
\nBa\u011flanma enerjisi ve R oran\u0131na dayanarak, TLR2 (5D3I), iNOS (2Y37), PI3K (1E7V), Keap1 (4L7B) ve IKK \u03b2 (3RZF) dahil olmak \u00fczere sargentol\u00fcn potansiyel hedefleri belirlenmi\u015ftir. Keap1-Nrf2-ARE sinyal yolu, ROS hasar\u0131na kar\u015f\u0131 h\u00fccre savunmas\u0131 i\u00e7in en \u00f6nemli mekanizmalardan biridir. Keap1 (4L7B) ve sargentol aras\u0131ndaki ba\u011flanma enerjisinin -7,2 kcal\/mol olmas\u0131, sargentol\u00fcn Keap1 proteinine kar\u015f\u0131 g\u00fc\u00e7l\u00fc aktivasyon yetene\u011fine ve Keap1-Nrf2-ARE sinyal yoluna dayal\u0131 potansiyel anti ROS etkilerine sahip olabilece\u011fini g\u00f6stermektedir. Bu spek\u00fclasyon Deney 2.2 kapsam\u0131ndaki verilerle tutarl\u0131d\u0131r. TLR2 (5D3I), iNOS (2Y37) ve PI3K (1E7V) ROS ve inflamasyon i\u00e7in \u00f6nemli d\u00fczenleyici proteinlerdir. Bu molek\u00fcler kenetlenmede, sargentole ba\u011flanma enerjileri s\u0131ras\u0131yla -7,7, -7,7 ve -7,3 kcal\/mol olmu\u015ftur. \u00d6zellikle ilki i\u00e7in ba\u011flanma g\u00fcc\u00fc orijinal ligand\u0131nkini (-7,5 kcal\/mol) a\u015fm\u0131\u015ft\u0131r. Bu da sargentol\u00fcn oksidatif stres inflamasyon oksidatif stres d\u00f6ng\u00fcs\u00fcn\u00fc inhibe ederek n\u00f6roprotektif etkiler g\u00f6sterebilece\u011fini d\u00fc\u015f\u00fcnd\u00fcrmektedir. \u00d6nceki \u00e7al\u0131\u015fmalarda, sargentol\u00fcn farelerde kulak \u015fi\u015fmesini \u00f6nemli \u00f6l\u00e7\u00fcde engelleyebildi\u011fi ve anti-enflamatuar aktivitesinin bu \u00e7al\u0131\u015fmadaki molek\u00fcler yerle\u015ftirme tahminiyle tutarl\u0131 oldu\u011fu bulunmu\u015ftur. Ba\u011flanma enerjisine g\u00f6re, sargentol eksitotoksisiteyi inhibe etme ve apoptoz genlerini d\u00fczenleme konusunda zay\u0131f bir yetene\u011fe sahiptir. Gelecekteki ara\u015ft\u0131rmalar sargentol\u00fcn anti-enflamatuar aktivitesi ve mekanizmas\u0131 \u00fczerine odaklanacakt\u0131r.<\/p>","protected":false},"excerpt":{"rendered":"

K\u0131rm\u0131z\u0131 asmadaki sargentol\u00fcn PC12 h\u00fccreleri \u00fczerindeki n\u00f6roprotektif etkisi ve molek\u00fcler kenetlenme \u00e7al\u0131\u015fmas\u0131 N\u00f6ronlar\u0131n hasar\u0131 ve \u00f6l\u00fcm\u00fc, esas olarak oksidatif stres, n\u00f6roinflamasyon, eksitotoksisite, kalsiyum iyonu (Ca2+) a\u015f\u0131r\u0131 y\u00fcklenmesi, mitokondriyal hasar, apoptotik proteinler, otofaji vb. dahil olmak \u00fczere \u00e7ok \u00e7e\u015fitli s\u00fcre\u00e7leri i\u00e7erir. N\u00f6roproteksiyon, n\u00f6rolojik hastal\u0131klar\u0131n \u00f6nlenmesi ve tedavisinde \u00f6nemli bir ba\u011flant\u0131d\u0131r [...]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"\nThe neuroprotective effect and molecular docking study of sargentol in red vine on PC12 cells - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/tr\/pc12-hucreleri-uzerinde-kirmizi-asma-icinde-sargentol\/\" \/>\n<meta property=\"og:locale\" content=\"tr_TR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"The neuroprotective effect and molecular docking study of sargentol in red vine on PC12 cells - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"The neuroprotective effect and molecular docking study of sargentol in red vine on PC12 cells The damage and death of neurons involve a wide range of processes, mainly including oxidative stress, neuroinflammation, excitotoxicity, calcium ion (Ca2+) overload, mitochondrial damage, apoptotic proteins, autophagy, etc. Neuroprotection is a key link in the prevention and treatment of neurological […]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/longchangextracts.com\/tr\/pc12-hucreleri-uzerinde-kirmizi-asma-icinde-sargentol\/\" \/>\n<meta property=\"og:site_name\" content=\"China Chemical Manufacturer\" \/>\n<meta property=\"article:published_time\" content=\"2024-08-14T19:43:48+00:00\" \/>\n<meta name=\"author\" content=\"longcha9\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Yazan:\" \/>\n\t<meta name=\"twitter:data1\" content=\"longcha9\" \/>\n\t<meta name=\"twitter:label2\" content=\"Tahmini okuma s\u00fcresi\" \/>\n\t<meta name=\"twitter:data2\" content=\"3 dakika\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/longchangextracts.com\/sargentol-in-red-vine-on-pc12-cells\/\",\"url\":\"https:\/\/longchangextracts.com\/sargentol-in-red-vine-on-pc12-cells\/\",\"name\":\"The neuroprotective effect and molecular docking study of sargentol in red vine on PC12 cells - 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