Paeonia lactiflora ve i\u015flenmi\u015f \u00fcr\u00fcnlerinin kalitesi \u00fczerine HPLC karakteristik spektrumu ile i\u00e7erik tayini kullan\u0131larak kar\u015f\u0131la\u015ft\u0131rmal\u0131 \u00e7al\u0131\u015fma
\nBeyaz \u015fakay\u0131k, Ranunculaceae familyas\u0131ndan bir bitki olan Paeonia lactiflora Pall'\u0131n kuru k\u00f6k\u00fcd\u00fcr. Do\u011fas\u0131 gere\u011fi ac\u0131, ek\u015fi ve hafif so\u011fuktur. Karaci\u011fer ve dalak meridyenlerine geri d\u00f6ner. Kan\u0131 besleyici ve meridyenleri d\u00fczenleyici, yini tonifiye edici ve terlemeyi durdurucu, karaci\u011feri yumu\u015fat\u0131c\u0131 ve a\u011fr\u0131y\u0131 dindirici ve karaci\u011fer yang\u0131n\u0131 sakinle\u015ftirici etkileri vard\u0131r. Kan eksikli\u011fi ve sararma, adet bozukluklar\u0131, spontan terleme, gece terlemeleri, kaburga a\u011fr\u0131s\u0131, kar\u0131n a\u011fr\u0131s\u0131, uzuv spazmlar\u0131, ba\u015f a\u011fr\u0131s\u0131 ve ba\u015f d\u00f6nmesi i\u00e7in kullan\u0131l\u0131r. Paeonia lactiflora'n\u0131n ana etken maddesi Paeonia lactiflora'n\u0131n toplam glukozitleridir (TGP). TGP, paeoniflorin, paeoniflorin, benzoil paeoniflorin ve anti-enflamatuar, ba\u011f\u0131\u015f\u0131kl\u0131k d\u00fczenleyici ve hepatoprotektif etkileri olan di\u011fer bile\u015fenlerin bir kar\u0131\u015f\u0131m\u0131d\u0131r. Kar\u0131\u015ft\u0131r\u0131larak k\u0131zart\u0131lm\u0131\u015f beyaz \u015fakay\u0131k ve \u015farap beyaz \u015fakay\u0131k, beyaz \u015fakay\u0131\u011f\u0131n i\u015flenmi\u015f \u00fcr\u00fcnleridir. Modern farmakolojik ara\u015ft\u0131rmalar, i\u015flenmi\u015f beyaz \u015fakay\u0131klar\u0131n farmakolojik etkilerinin genel olarak artt\u0131\u011f\u0131n\u0131 ve i\u015flenmi\u015f \u00fcr\u00fcnlerin analjezik etkisinin ham \u00fcr\u00fcnlerden daha \u00f6nemli oldu\u011funu g\u00f6stererek beyaz \u015fakay\u0131k i\u015flemenin \u00f6nemini tam olarak ortaya koymu\u015ftur. Ulusal \u00c7in T\u0131bb\u0131 \u0130\u015fleme Standartlar\u0131 \u015farapta beyaz \u015fakay\u0131k, kar\u0131\u015ft\u0131r\u0131larak k\u0131zart\u0131lm\u0131\u015f beyaz \u015fakay\u0131k ve toprakta kar\u0131\u015ft\u0131r\u0131larak k\u0131zart\u0131lm\u0131\u015f beyaz \u015fakay\u0131k i\u00e7in i\u015fleme y\u00f6ntemleri i\u00e7ermektedir; \"Jingyue Quanshu\" ve Jiao Shude'nin \"\u0130la\u00e7 Deneyimi \u00dczerine On Ders\" adl\u0131 eserlerinin her ikisi de kar\u0131\u015ft\u0131r\u0131larak k\u0131zart\u0131lm\u0131\u015f beyaz \u015fakay\u0131k kullan\u0131m\u0131n\u0131 kaydetmektedir. Beyaz \u015fakay\u0131k k\u0131zart\u0131ld\u0131ktan sonra so\u011futma \u00f6zellikleri hafifler ve kan ve kollateralleri besleyici, dalak a\u011fr\u0131s\u0131n\u0131 hafifletici etkilere sahiptir ve dalak eksikli\u011fi veya karaci\u011fer dalak uyumsuzlu\u011fundan kaynaklanan ishal ve kar\u0131n a\u011fr\u0131s\u0131n\u0131 tedavi etmek i\u00e7in kullan\u0131labilir. \"Bian Que Xin Shu\" kitab\u0131 beyaz \u015fakay\u0131\u011f\u0131n \"\u015farapla kar\u0131\u015ft\u0131r\u0131larak k\u0131zart\u0131lmas\u0131\" y\u00f6ntemine \u00f6nc\u00fcl\u00fck ederken, \"Tang Ye Ben Cao\" beyaz \u015fakay\u0131k \u015farab\u0131n\u0131n meridyende bekletilmesinin merkezi kar\u0131n a\u011fr\u0131s\u0131n\u0131 hafifletebilece\u011fini kaydeder; The Compendium of Materia Medica \"orta so\u011fuktan ka\u00e7\u0131nanlar\u0131n \u015farapla kar\u0131\u015ft\u0131rarak k\u0131zartmas\u0131 gerekti\u011fini\" ve \"yin'i beslemek i\u00e7in \u015farapla kar\u0131\u015ft\u0131rarak k\u0131zart\u0131lmas\u0131 gerekti\u011fini\" belirtir. Beyaz \u015fakay\u0131k \u015farab\u0131 ile kar\u0131\u015ft\u0131r\u0131larak k\u0131zart\u0131ld\u0131ktan sonra, ek\u015fi ve so\u011fuk do\u011fas\u0131 azalt\u0131labilir ve orta, yava\u015f ve acil olan\u0131 uyumlu hale getirmede iyidir. Genellikle kaburga a\u011fr\u0131s\u0131, kar\u0131n a\u011fr\u0131s\u0131, \u00f6zellikle do\u011fum sonras\u0131 kar\u0131n a\u011fr\u0131s\u0131 i\u00e7in kullan\u0131l\u0131r.<\/p>\n
K\u00fck\u00fcrt f\u00fcmigasyonu, b\u00f6cek istilas\u0131n\u0131, k\u00fcf olu\u015fumunu \u00f6nlemek ve geleneksel \u00c7in t\u0131bbi malzemelerinin saklama s\u00fcresini uzatmak i\u00e7in yayg\u0131n olarak kullan\u0131lan bir y\u00f6ntemdir. Ancak, k\u00fck\u00fcrt f\u00fcmigasyonu baz\u0131 geleneksel \u00c7in t\u0131bbi malzemelerinde a\u015f\u0131r\u0131 SO2 kal\u0131nt\u0131s\u0131na neden olabilir, bu da etkili bile\u015fenlerde ve i\u00e7eriklerinde de\u011fi\u015fikliklere yol a\u00e7arak etkinliklerini azalt\u0131r ve hatta onlar\u0131 \"zehir\" haline getirerek geleneksel \u00c7in t\u0131bbi malzemelerinin kalitesini ve g\u00fcvenli\u011fini ciddi \u015fekilde etkiler. Paeonia lactiflora'n\u0131n modern i\u015fleme \u00e7al\u0131\u015fmalar\u0131, s\u00fclf\u00fcr f\u00fcmigasyonunun Paeonia lactiflora'daki paeoniflorin i\u00e7eri\u011finde bir azalmaya yol a\u00e7abilece\u011fini g\u00f6stermi\u015ftir. K\u00fck\u00fcrt f\u00fcmigasyonunun baz\u0131 paeoniflorinleri yeni bir bile\u015fen olan paeoniflorin s\u00fclfite d\u00f6n\u00fc\u015ft\u00fcrebilece\u011fi ve b\u00f6ylece paeoniflorin i\u00e7eri\u011fini azaltabilece\u011fi tahmin edilmektedir.<\/p>\n
Bu \u00e7al\u0131\u015fma, Paeonia lactiflora t\u0131bbi malzemeleri ve i\u015flenmi\u015f \u00fcr\u00fcnleri i\u00e7in \u00c7in Farmakopesi standard\u0131n\u0131n 2020 bask\u0131s\u0131na dayanarak, HPLC y\u00fcksek performansl\u0131 s\u0131v\u0131 kromatografisi kullanarak Paeonia lactiflora, Paeonia lactiflora \u015farab\u0131, Paeonia lactiflora k\u0131zartmas\u0131 ve Paeonia lactiflora k\u00fck\u00fcrt t\u00fcts\u00fclenmesinin karakteristik spektrumlar\u0131n\u0131 olu\u015fturmu\u015f ve alt\u0131 aktif bile\u015fen tan\u0131mlam\u0131\u015ft\u0131r: gallik asit, kate\u015finler, paeoniflorin, paeoniflorin, 1,2,3,4,6-pentagalloil glukoz ve benzoil paeoniflorin; Beyaz \u015fakay\u0131k, \u015farap beyaz \u015fakay\u0131k, k\u0131zart\u0131lm\u0131\u015f beyaz \u015fakay\u0131k ve k\u00fck\u00fcrt t\u00fcts\u00fclenmi\u015f beyaz \u015fakay\u0131kta yukar\u0131da belirtilen alt\u0131 aktif bile\u015fenin i\u00e7eri\u011fini e\u015f zamanl\u0131 olarak \u00f6l\u00e7mek ve karakteristik spektrumlar\u0131n benzerlik hesaplamas\u0131 ve kemometrik analiz y\u00f6ntemlerini kullanarak beyaz \u015fakay\u0131k, i\u015flenmi\u015f \u00fcr\u00fcnler ve k\u00fck\u00fcrt t\u00fcts\u00fclenmi\u015f \u00fcr\u00fcnlerin kalitesini de\u011ferlendirmek. Bu \u00e7al\u0131\u015fma, beyaz \u015fakay\u0131k, i\u015flenmi\u015f \u00fcr\u00fcnler ve k\u00fck\u00fcrt t\u00fcts\u00fclenmi\u015f \u00fcr\u00fcnlerin tan\u0131mlanmas\u0131 ve kalite de\u011ferlendirmesi i\u00e7in bir referans sa\u011flamaktad\u0131r.<\/p>\n
Test \u00e7\u00f6zeltisinin haz\u0131rlanmas\u0131. Bu \u00e7al\u0131\u015fmada, farkl\u0131 konsantrasyonlardaki metanol ve etanol\u00fcn ekstraksiyon etkinli\u011fi tek fakt\u00f6r analizi ile ara\u015ft\u0131r\u0131lm\u0131\u015ft\u0131r. Kromatografik pik \"toplam pik alan\u0131\/\u00f6rnek boyutu\" karakteristik spektrum i\u00e7in de\u011ferlendirme indeksi olarak kullan\u0131lm\u0131\u015f ve gallik asit, kate\u015finler, paeoniflorin, paeoniflorin, 1,2,3,4,6-pentagalloil glukoz ve benzoil paeoniflorin toplam i\u00e7eri\u011fi i\u00e7erik belirleme indeksi olarak kullan\u0131lm\u0131\u015ft\u0131r. Son olarak, seyreltik etanol ekstraksiyon \u00e7\u00f6z\u00fcc\u00fcs\u00fc olarak belirlenmi\u015f; iki ekstraksiyon y\u00f6ntemi, \u0131s\u0131tma refl\u00fcs\u00fc ve ultrasonik i\u015flem \u00fczerinde kar\u015f\u0131la\u015ft\u0131rmal\u0131 bir \u00e7al\u0131\u015fma yap\u0131lm\u0131\u015f ve farkl\u0131 ekstraksiyon y\u00f6ntemlerinin Paeonia lactiflora'n\u0131n ekstraksiyon verimlili\u011fi \u00fczerinde \u00e7ok az etkisi oldu\u011fu bulunmu\u015ftur. Bu nedenle, i\u015flemin basitli\u011fi g\u00f6z \u00f6n\u00fcnde bulundurularak ultrasonik i\u015flem se\u00e7ilmi\u015ftir; Ayn\u0131 zamanda, numunelerin ultrasonik i\u015flem s\u00fcresi (15, 30, 45 dakika) ara\u015ft\u0131r\u0131lm\u0131\u015f ve 30 dakikal\u0131k ultrasonik i\u015flemin \u00e7e\u015fitli bile\u015fenleri tamamen \u00e7\u0131karabilece\u011fi bulunmu\u015ftur. Yukar\u0131daki ara\u015ft\u0131rmaya dayanarak, beyaz \u015fakay\u0131k test \u00e7\u00f6zeltisi i\u00e7in en iyi haz\u0131rlama y\u00f6nteminin 50 mL seyreltik etanol\u00fcn 30 dakika boyunca ultrasonik i\u015flemle (g\u00fc\u00e7 250W, frekans 40kHz) muamele edilmesi oldu\u011fu belirlenmi\u015ftir.<\/p>\n
\u00d6zellik haritalar\u0131n\u0131n olu\u015fturulmas\u0131 ve analizi. Karakteristik spektrumlar\u0131n ara\u015ft\u0131rma sonu\u00e7lar\u0131, beyaz \u015fakay\u0131k, k\u0131zarm\u0131\u015f beyaz \u015fakay\u0131k ve \u015farap beyaz \u015fakay\u0131\u011f\u0131n\u0131n HPLC karakteristik spektrumlar\u0131nda 6 ortak pik ve k\u00fck\u00fcrt t\u00fcts\u00fclenmi\u015f beyaz \u015fakay\u0131\u011f\u0131n HPLC karakteristik spektrumlar\u0131nda 7 ortak pik oldu\u011funu g\u00f6stermektedir. \u00c7al\u0131\u015fma s\u00fcresini k\u0131saltmak ve i\u015f verimlili\u011fini art\u0131rmak i\u00e7in numune tespiti i\u00e7in farkl\u0131 \u00fcreticilerin Agilent, Waters ve Thermo cihazlar\u0131n\u0131n kullan\u0131lmas\u0131 nedeniyle, ayn\u0131 kromatografik kolon kullan\u0131lsa bile, karakteristik spektrumdaki kromatografik piklerin al\u0131konma s\u00fcresi, farkl\u0131 \u00fcreticiler aras\u0131ndaki makine \u00f6l\u00fc hacmindeki farkl\u0131l\u0131klar nedeniyle de\u011fi\u015febilir. Pik 4 S piki olarak al\u0131n\u0131r ve ba\u011f\u0131l al\u0131konma s\u00fcresi hesaplan\u0131rsa, her bir karakteristik pikin ba\u011f\u0131l al\u0131konma s\u00fcresi nispeten sabit olacakt\u0131r. Pik 7, Paeonia lactiflora'n\u0131n k\u00fck\u00fcrt f\u00fcmigasyonundan sonra olu\u015fur ve bunun paeoniflorin s\u00fclfit oldu\u011fu tahmin edilmektedir. Paeonia lactiflora, k\u0131zarm\u0131\u015f Paeonia lactiflora ve \u015farap Paeonia lactiflora'n\u0131n alt\u0131 karakteristik bile\u015feni referans standartlarla tan\u0131mlanm\u0131\u015ft\u0131r: gallik asit, kate\u015finler, paeoniflorin, paeoniflorin, 1,2,3,4,6-pentagalloil glukoz ve benzoil paeoniflorin. OPLS-DA analizi, Paeonia lactiflora'n\u0131n i\u015flenmesi ve k\u00fck\u00fcrt f\u00fcmigasyonundan sonra pik 7, pik 4 (paeoniflorin) ve pik 5'te (1,2,3,4,6-pentagalloil glukoz) \u00f6nemli de\u011fi\u015fiklikler oldu\u011funu g\u00f6steren \u00fc\u00e7 farkl\u0131 biyobelirte\u00e7 tan\u0131mlam\u0131\u015ft\u0131r.<\/p>\n
Paeonia lactiflora'n\u0131n k\u0131zartma, \u015farapta kavurma ve k\u00fck\u00fcrt f\u00fcmigasyonundan sonra kimyasal bile\u015fimindeki de\u011fi\u015fiklikler. Beyaz \u015fakay\u0131k, k\u0131zarm\u0131\u015f beyaz \u015fakay\u0131k, \u015farap beyaz \u015fakay\u0131k ve k\u00fck\u00fcrt t\u00fcts\u00fclenmi\u015f beyaz \u015fakay\u0131kta bilinen alt\u0131 bile\u015fenin i\u00e7erik belirleme sonu\u00e7lar\u0131, gallik asit hari\u00e7 di\u011fer be\u015f bile\u015fenin de\u011fi\u015fen derecelerde de\u011fi\u015fiklik g\u00f6sterdi\u011fini ortaya koymu\u015ftur. K\u0131zartma ve \u015farapta kavurma i\u015flemlerinden sonra beyaz \u015fakay\u0131ktaki paeoniflorin i\u00e7eri\u011fi azal\u0131rken, paeoniflorin lakton ve benzoil paeoniflorin i\u00e7eri\u011fi artm\u0131\u015ft\u0131r. Bunun nedeni, beyaz \u015fakay\u0131\u011f\u0131n i\u015flenmesi s\u0131ras\u0131nda paeoniflorinin paeoniflorin lakton ve benzoil paeoniflorine d\u00f6n\u00fc\u015fmesi olabilir; K\u00fck\u00fcrt f\u00fcmigasyonundan sonra, paeoniflorin i\u00e7eri\u011finin azald\u0131\u011f\u0131, bunun da k\u00fck\u00fcrt f\u00fcmigasyonundan sonra beyaz \u015fakay\u0131\u011f\u0131n paeoniflorin s\u00fclfite d\u00f6n\u00fc\u015fmesinden kaynakland\u0131\u011f\u0131 tahmin edilmektedir. Kate\u015finler \u0131s\u0131ya maruz kald\u0131klar\u0131nda karars\u0131zd\u0131rlar. Bu nedenle, k\u0131zartma ve \u015farap kavurma i\u015flemleri beyaz \u015fakay\u0131ktaki kate\u015fin i\u00e7eri\u011fini \u00f6nemli \u00f6l\u00e7\u00fcde azalt\u0131rken, k\u00fck\u00fcrt f\u00fcmigasyon i\u015flemi belirgin bir \u0131s\u0131ya maruz kalmamas\u0131 nedeniyle kate\u015fin i\u00e7eri\u011finde \u00f6nemli de\u011fi\u015fiklikler g\u00f6stermemi\u015ftir. Buna ek olarak, \u015farap kavurma ve k\u00fck\u00fcrt f\u00fcmigasyonundan sonra, 1,2,3,4,6-pentagalloilglukoz i\u00e7eri\u011fi \u00f6nemli \u00f6l\u00e7\u00fcde artm\u0131\u015ft\u0131r; bunun, 1,2,3,4,6-pentagalloilglukozun d\u00f6n\u00fc\u015f\u00fcm\u00fcn\u00fc te\u015fvik eden Paeonia lactiflora'n\u0131n i\u015flenmesi s\u0131ras\u0131nda yard\u0131mc\u0131 malzemelerdeki \u0131s\u0131 ve baz\u0131 kimyasal bile\u015fenlerin katalitik etkisinden kaynakland\u0131\u011f\u0131 sonucuna var\u0131lm\u0131\u015ft\u0131r. Spesifik mekanizman\u0131n daha fazla ara\u015ft\u0131r\u0131lmas\u0131 gerekmektedir.<\/p>\n
Geleneksel \u00c7in t\u0131bb\u0131 teorisine g\u00f6re, geleneksel \u00c7in t\u0131bb\u0131n\u0131n farkl\u0131 i\u015flenmi\u015f \u00fcr\u00fcnlerinin klinik uygulamalar\u0131nda, i\u00e7 kimyasal bile\u015fimlerindeki farkl\u0131l\u0131klarla ilgili farkl\u0131l\u0131klar olmal\u0131d\u0131r. Paeonia lactiflora kan\u0131 besleyici, yini besleyici, karaci\u011feri koruyucu ve spazmlar\u0131 giderici etkilere sahiptir. Kar\u0131\u015ft\u0131rarak k\u0131zartt\u0131ktan sonra so\u011futma \u00f6zellikleri hafifler, dalak rahatlat\u0131c\u0131 ve a\u011fr\u0131 kesici etkileri artar. Alkolle kavrulduktan sonra ek\u015fi ve so\u011fuk \u00f6zellikleri azal\u0131r, \u0131l\u0131ml\u0131 ve acil etkileri artar. Bu durum Paeonia lactiflora'daki paeoniflorin i\u00e7eri\u011finin azalmas\u0131, glikozit laktonlar ve benzoil paeoniflorin i\u00e7eri\u011finin artmas\u0131 ile ilgili olabilir. Paeonia lactiflora'n\u0131n k\u00fck\u00fcrt f\u00fcmigasyonu b\u00f6cekleri \u00f6nleyebilse, k\u00fcflenmeyi ve \u00e7\u00fcr\u00fcmeyi \u00f6nleyebilse de, a\u015f\u0131r\u0131 k\u00fck\u00fcrt f\u00fcmigasyonu paeoniflorin i\u00e7eri\u011finde \u00f6nemli bir azalmaya yol a\u00e7ar, bu da baz\u0131 paeoniflorinlerin paeoniflorin s\u00fclfite d\u00f6n\u00fc\u015fmesinden kaynaklanabilir, dolay\u0131s\u0131yla t\u0131bbi malzemeleri ciddi \u015fekilde etkiler. Kaynatma par\u00e7alar\u0131n\u0131n kalitesi s\u0131k\u0131 bir \u015fekilde kontrol edilmelidir, bu nedenle k\u00fck\u00fcrt f\u00fcmigasyon i\u015flemi s\u0131k\u0131 bir \u015fekilde kontrol edilmelidir. Daha sonraki a\u015famada, Paeonia lactiflora'n\u0131n k\u00fck\u00fcrt f\u00fcmigasyon s\u00fcreci hakk\u0131nda daha fazla ara\u015ft\u0131rma yap\u0131lacak, farkl\u0131 derecelerde k\u00fck\u00fcrt f\u00fcmigasyonunun Paeonia lactiflora'n\u0131n etkili bile\u015fenleri \u00fczerindeki etkileri ara\u015ft\u0131r\u0131lacak ve optimum k\u00fck\u00fcrt f\u00fcmigasyon s\u00fcreci belirlenecektir.<\/p>","protected":false},"excerpt":{"rendered":"
Paeonia lactiflora ve i\u015flenmi\u015f \u00fcr\u00fcnlerinin kalitesi \u00fczerine HPLC karakteristik spektrumu ile i\u00e7erik tayini kullan\u0131larak kar\u015f\u0131la\u015ft\u0131rmal\u0131 \u00e7al\u0131\u015fma Beyaz \u015fakay\u0131k, Ranunculaceae familyas\u0131ndan bir bitki olan Paeonia lactiflora Pall'\u0131n kuru k\u00f6k\u00fcd\u00fcr. Do\u011fas\u0131 gere\u011fi ac\u0131, ek\u015fi ve hafif so\u011fuktur. Karaci\u011fer ve dalak meridyenlerine geri d\u00f6n\u00fcn. Bu [...]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"\n