Kemometri ile birle\u015ftirilmi\u015f k\u0131z\u0131l\u00f6tesi spektroskopi kullan\u0131larak ay\u0131 safras\u0131 tozunun orijinalli\u011finin ve ta\u011f\u015fi\u015finin h\u0131zl\u0131 tespiti
\nAy\u0131 safras\u0131 tozu, ay\u0131 ailesinin bir \u00fcyesi olan Selenaretos thibetanus Cuvies'in safra kesesi ameliyat\u0131ndan elde edilen kurutulmu\u015f bir \u00fcr\u00fcnd\u00fcr. Is\u0131y\u0131 temizleme, karaci\u011feri sakinle\u015ftirme ve g\u00f6r\u00fc\u015f\u00fc iyile\u015ftirme etkilerine sahiptir ve \u00c7in'deki d\u00f6rt de\u011ferli hayvansal t\u0131bbi malzemeden biridir. Ay\u0131 safra tozunun \u00f6zel kayna\u011f\u0131 ve y\u00fcksek fiyat\u0131 nedeniyle, piyasada di\u011fer hayvan safra tozlar\u0131n\u0131n ay\u0131 safra tozu olarak sat\u0131ld\u0131\u011f\u0131 durumlar vard\u0131r. Buna ek olarak, ara\u015ft\u0131rma grubumuz pazar ara\u015ft\u0131rmas\u0131n\u0131n ilk a\u015famas\u0131nda, domuz safras\u0131 tozu ve inek safras\u0131 tozunun \u00f6nemli \u00fcretimi ve d\u00fc\u015f\u00fck fiyat\u0131 nedeniyle, g\u00f6r\u00fcn\u00fcm ve \u00f6zelliklerinin ay\u0131 safras\u0131 tozuna benzedi\u011fini tespit etti. Ay\u0131 safras\u0131 tozu olarak taklit edilmelerinin yan\u0131 s\u0131ra, genellikle sat\u0131\u015f i\u00e7in ay\u0131 safras\u0131 tozuna kar\u0131\u015ft\u0131r\u0131l\u0131rlar ve sahte \u00fcr\u00fcnlerle kar\u015f\u0131la\u015ft\u0131r\u0131ld\u0131\u011f\u0131nda, ta\u011f\u015fi\u015f edilmi\u015f \u00fcr\u00fcnlerin tespit edilme zorlu\u011fu daha y\u00fcksektir. Bu olgu, piyasada bulunan ay\u0131 safras\u0131 tozunun kalitesinin dengesiz olmas\u0131na yol a\u00e7arak klinik etkinli\u011fi ve t\u00fcketici haklar\u0131n\u0131 ciddi \u015fekilde etkilemektedir. Bu nedenle, ay\u0131 safra tozunun orijinalli\u011finin belirlenmesi ve ta\u011f\u015fi\u015f oran\u0131n\u0131n tahmin edilmesi, ay\u0131 safra tozunun kalite kontrol\u00fc i\u00e7in b\u00fcy\u00fck \u00f6nem ta\u015f\u0131maktad\u0131r.
\nAy\u0131 safra tozu i\u00e7in yayg\u0131n olarak kullan\u0131lan tan\u0131mlama y\u00f6ntemleri aras\u0131nda ince tabaka kromatografisi, y\u00fcksek performansl\u0131 s\u0131v\u0131 kromatografisi ve spesifik polimeraz zincir reaksiyonu (PCR) yer almaktad\u0131r, ancak bu y\u00f6ntemlerin uzun zaman t\u00fcketimi, karma\u015f\u0131k i\u015flem ve numune \u00fczerinde y\u0131k\u0131c\u0131 etkiler gibi sorunlar\u0131 vard\u0131r. Ayr\u0131ca, \u00f6rnek boyutu b\u00fcy\u00fck oldu\u011funda baz\u0131 y\u00f6ntemler b\u00fcy\u00fck miktarda organik reaktif gerektirir. Yak\u0131n k\u0131z\u0131l\u00f6tesi spektroskopisi, h\u0131zl\u0131 analiz h\u0131z\u0131, minimum numune tahribat\u0131 ve \u00f6n i\u015fleme gerek olmamas\u0131 gibi avantajlara sahiptir. Geleneksel \u00c7in t\u0131bb\u0131n\u0131n orijinalli\u011finin ve ta\u011f\u015fi\u015finin belirlenmesinde yayg\u0131n olarak kullan\u0131lmaktad\u0131r. Bununla birlikte, ay\u0131 safras\u0131 tozunun g\u00fc\u00e7l\u00fc higroskopikli\u011fi nedeniyle, yak\u0131n k\u0131z\u0131l\u00f6tesi spektroskopi analizi i\u00e7in kullan\u0131ld\u0131\u011f\u0131nda baz\u0131 numuneleri kaybetme e\u011filimi g\u00f6sterir. Bu nedenle, safra t\u0131bbi malzemelerine uyguland\u0131\u011f\u0131nda bu y\u00f6ntemin belirli s\u0131n\u0131rlamalar\u0131 vard\u0131r. Yak\u0131n k\u0131z\u0131l\u00f6tesi spektroskopisi ile kar\u015f\u0131la\u015ft\u0131r\u0131ld\u0131\u011f\u0131nda, k\u0131z\u0131l\u00f6tesi spektroskopisi ayn\u0131 zamanda h\u0131zl\u0131 ve hassas analiz \u00f6zelliklerine sahiptir ve \u00e7ok k\u00fc\u00e7\u00fck bir \u00f6rnek boyutu gerektirir, bu da onu de\u011ferli t\u0131bbi malzemelerin kalite kontrol\u00fc i\u00e7in daha uygun hale getirir. \u015eu anda, ay\u0131 safras\u0131 tozunu tan\u0131mlamak i\u00e7in k\u0131z\u0131l\u00f6tesi spektroskopinin kullan\u0131m\u0131na ili\u015fkin \u00e7ok az rapor bulunmaktad\u0131r. Sadece Yan ve arkada\u015flar\u0131 ay\u0131 safras\u0131 tozunun k\u0131z\u0131l\u00f6tesi spektrumlar\u0131n\u0131 di\u011fer \u00fc\u00e7 hayvan safras\u0131 tozuyla kar\u015f\u0131la\u015ft\u0131rm\u0131\u015ft\u0131r, ancak \u00f6rneklem boyutlar\u0131 k\u00fc\u00e7\u00fckt\u00fcr ve ta\u011f\u015fi\u015f davran\u0131\u015f\u0131na dikkat edilmemi\u015f ve kemometri ile birlikte sonu\u00e7lar\u0131n g\u00f6rselle\u015ftirilmesi yap\u0131lmam\u0131\u015ft\u0131r. Bu \u00e7al\u0131\u015fmada, farkl\u0131 oranlarda ay\u0131 safra tozu, domuz safra tozu ve inek safra tozu i\u00e7eren ta\u011f\u015fi\u015f edilmi\u015f numuneler haz\u0131rlanm\u0131\u015ft\u0131r. Orijinal \u00fcr\u00fcnlerin, 7 \u00e7e\u015fit ta\u011f\u015fi\u015f edilmi\u015f \u00fcr\u00fcn\u00fcn ve 2 \u00e7e\u015fit ta\u011f\u015fi\u015f edilmi\u015f \u00fcr\u00fcn\u00fcn k\u0131z\u0131l\u00f6tesi spektrumlar\u0131 toplanm\u0131\u015ft\u0131r. Elde edilen spektrumlar\u0131 optimize etmek i\u00e7in taban \u00e7izgisi d\u00fczeltme, yumu\u015fatma, farkl\u0131la\u015ft\u0131rma ve di\u011fer \u00f6n i\u015fleme y\u00f6ntemleri kullan\u0131lm\u0131\u015ft\u0131r. Ortogonal k\u0131smi en k\u00fc\u00e7\u00fck kareler diskriminant analizi (OPLS-DA) ve k\u0131smi en k\u00fc\u00e7\u00fck kareler regresyonu (PLSR) y\u00f6ntemleri ile birlikte, ay\u0131 safra tozunun orijinalli\u011finin belirlenmesi ve ta\u011f\u015fi\u015f oranlar\u0131n\u0131n tahmin edilmesi i\u00e7in k\u0131z\u0131l\u00f6tesi spektrumlar\u0131n kullan\u0131lmas\u0131n\u0131n fizibilitesi ara\u015ft\u0131r\u0131lm\u0131\u015f ve ay\u0131 safra tozu ve di\u011fer de\u011ferli t\u0131bbi malzemelerin kalite kontrol\u00fc i\u00e7in bir referans sa\u011flanm\u0131\u015ft\u0131r.<\/p>\n
Bu \u00e7al\u0131\u015fmada, ilk kez, ger\u00e7ek ay\u0131 safra tozu, farkl\u0131 sahte \u00fcr\u00fcn kategorileri ve ta\u011f\u015fi\u015f edilmi\u015f \u00fcr\u00fcnler i\u00e7in OPLS-DA kalitatif modellerinin yan\u0131 s\u0131ra iki t\u00fcr ta\u011f\u015fi\u015f edilmi\u015f ay\u0131 safra tozu ta\u011f\u015fi\u015f oran\u0131 i\u00e7in PLSR kantitatif modelleri olu\u015fturmak \u00fczere kemometri ile birlikte k\u0131z\u0131l\u00f6tesi spektroskopi kullan\u0131lm\u0131\u015ft\u0131r. Farkl\u0131 spektral \u00f6n i\u015fleme y\u00f6ntemleri sayesinde, orijinal, sahte ve ta\u011f\u015fi\u015f edilmi\u015f ay\u0131 safra tozu i\u00e7in olu\u015fturulan tan\u0131mlama modellerinin yan\u0131 s\u0131ra, kalibrasyon seti ve do\u011frulama seti i\u00e7in ta\u011f\u015fi\u015f edilmi\u015f \u00fcr\u00fcn kategorisi modeli ve ta\u011f\u015fi\u015f edilmi\u015f \u00fcr\u00fcn kategorisi modelinin tan\u0131mlama do\u011frulu\u011fu 95%'yi a\u015fm\u0131\u015ft\u0131r; Ayr\u0131ca, iki t\u00fcrdeki ta\u011f\u015fi\u015f edilmi\u015f ay\u0131 safra tozu oran\u0131 i\u00e7in nicel modelin R2c ve R2v de\u011ferlerinin her ikisi de 0,95'ten b\u00fcy\u00fckt\u00fcr. Yukar\u0131daki sonu\u00e7lar, k\u0131z\u0131l\u00f6tesi spektroskopi teknolojisinin ay\u0131 safras\u0131 tozunun kalitesini h\u0131zl\u0131 bir \u015fekilde tespit edebilece\u011fini ve sonu\u00e7lar\u0131n iyi bir do\u011frulu\u011fa sahip oldu\u011funu g\u00f6stermektedir.<\/p>\n
Hayvan safras\u0131 t\u0131bbi malzemeleri i\u00e7in mevcut geleneksel analiz y\u00f6ntemleriyle kar\u015f\u0131la\u015ft\u0131r\u0131ld\u0131\u011f\u0131nda, k\u0131z\u0131l\u00f6tesi spektroskopi sadece sonu\u00e7lar\u0131n do\u011frulu\u011funu sa\u011flamakla kalmaz, ayn\u0131 zamanda zaman ve maliyet tasarrufu sa\u011flayarak de\u011ferli t\u0131bbi malzemelerin kalite kontrol\u00fcn\u00fc neredeyse tahribats\u0131z bir \u015fekilde ger\u00e7ekle\u015ftirir. Ara\u015ft\u0131rma grubumuz taraf\u0131ndan olu\u015fturulan mevcut numune toplama ve ay\u0131 safras\u0131 tozunun kantitatif modeline dayanarak, k\u0131z\u0131l\u00f6tesi spektroskopi m\u00fckemmel tespit kabiliyeti g\u00f6stermi\u015ftir. Bununla birlikte, spektral model \u00e7ok say\u0131da numuneye dayanmaktad\u0131r ve model do\u011frulu\u011funu ve kararl\u0131l\u0131\u011f\u0131n\u0131 art\u0131rmak i\u00e7in s\u00fcrekli numune takviyesi gerektirmektedir. Bu nedenle, gelecekte, ay\u0131 safra tozunun kalitesini daha do\u011fru bir \u015fekilde de\u011ferlendirmek i\u00e7in numune t\u00fcrlerini ve say\u0131lar\u0131n\u0131 daha da art\u0131raca\u011f\u0131z.<\/p>\n
Kemometrinin geli\u015fmesiyle birlikte, infrared spektroskopisi t\u0131bbi malzemelerin kalite kontrol\u00fcnde geni\u015f uygulama olanaklar\u0131na sahiptir. Bu \u00e7al\u0131\u015fma, de\u011ferli t\u0131bbi malzemelerin kalite kontrol\u00fc i\u00e7in basit ve h\u0131zl\u0131 yeni bir y\u00f6ntem sunmakta ve ayr\u0131ca ay\u0131 safras\u0131 tozunun kalite kontrol\u00fc i\u00e7in bir referans sa\u011flamaktad\u0131r.<\/p>","protected":false},"excerpt":{"rendered":"
Rapid detection of authenticity and adulteration of bear bile powder using infrared spectroscopy combined with chemometrics Bear bile powder is a dried product obtained from the gallbladder surgery of Selenaretos thibetanus Cuvies, a member of the bear family. It has the effects of clearing heat, calming the liver, and improving vision, and is one of […]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"\n