Pamuk \u00c7i\u00e7eklerinin Farkl\u0131 K\u0131s\u0131mlar\u0131ndaki Farkl\u0131l\u0131klar\u0131n Kimyasal \u00d6r\u00fcnt\u00fc Tan\u0131ma ve Entropi A\u011f\u0131rl\u0131kl\u0131 TOPSIS Y\u00f6ntemine Dayal\u0131 Analizi
\nPamuk \u00e7i\u00e7ekleri, pamuk bitkisi Gossampinus malabarica (DC.) Merr'in kuru \u00e7i\u00e7ekleridir. Do\u011fada serindirler ve kal\u0131n ba\u011f\u0131rsak meridyenine aittirler. Is\u0131 ve rutubeti giderici, detoksifiye edici etkileri vard\u0131r ve ishal, dizanteri, hemoroid ve kanama gibi hastal\u0131klar i\u00e7in yayg\u0131n olarak kullan\u0131l\u0131rlar. Pamuk \u00e7i\u00e7ekleri bol miktarda bulunur ve Yunnan, Hainan, Guangxi, Guangdong, Tayvan ve \u00c7in'in di\u011fer b\u00f6lgelerinde yayg\u0131n olarak da\u011f\u0131t\u0131l\u0131r. Genellikle \u00e7i\u00e7eklerin tam a\u00e7t\u0131\u011f\u0131 bahar aylar\u0131nda hasat edilirler. Modern ara\u015ft\u0131rmalar pamuk \u00e7i\u00e7eklerinin flavonoidler, fenilpropanoidler, fenolik asitler ve di\u011fer bile\u015fikler a\u00e7\u0131s\u0131ndan zengin oldu\u011funu g\u00f6stermi\u015ftir. Bunlar aras\u0131nda mangiferin ve rutin gibi flavonoid bile\u015fikler karaci\u011fer hasar\u0131, miyokardiyal iskemi-reperf\u00fczyon hasar\u0131, akci\u011fer kanseri ve di\u011fer hastal\u0131klar\u0131n tedavisinde iyi terap\u00f6tik etkilere sahiptir; Fenolik asitler g\u00fc\u00e7l\u00fc DPPH serbest radikal temizleme aktivitesine sahiptir ve protokate\u015fuik asit diyabette potansiyel hipoglisemik etkiye sahiptir. \u015eu anda, pamuk \u00e7i\u00e7eklerinin mikroskobik tan\u0131mlanmas\u0131, ince tabaka tan\u0131mlanmas\u0131 ve mangiferin i\u00e7eri\u011finin belirlenmesi \u00fczerine bir\u00e7ok \u00e7al\u0131\u015fma vard\u0131r, ancak pamuk \u00e7i\u00e7eklerinin HPLC parmak izi ve farkl\u0131 par\u00e7alar\u0131n kalite farkl\u0131l\u0131klar\u0131 hakk\u0131nda \u00e7ok az rapor vard\u0131r.<\/p>\n
Kapok \u00e7i\u00e7e\u011fi t\u0131bbi malzemelerinin kalitesinin yaln\u0131zca tek bir kimyasal bile\u015fene dayal\u0131 olarak de\u011ferlendirilmesinde baz\u0131 s\u0131n\u0131rlamalar vard\u0131r. Geleneksel \u00c7in t\u0131bb\u0131 parmak izi ve kemometrinin organik kombinasyonu, kapok \u00e7i\u00e7e\u011fi kimyasal bile\u015fenlerinin \u00e7e\u015fitlili\u011fini ve karma\u015f\u0131kl\u0131\u011f\u0131n\u0131 kapsaml\u0131 bir \u015fekilde yans\u0131tabilir ve kapok \u00e7i\u00e7e\u011finin farkl\u0131 k\u0131s\u0131mlar\u0131n\u0131n kalite farkl\u0131l\u0131klar\u0131n\u0131 sistematik olarak karakterize edebilir. Entropi a\u011f\u0131rl\u0131kl\u0131 optimal \u00e7\u00f6z\u00fcm mesafesi (TOPSIS) y\u00f6ntemi, birden fazla g\u00f6stergeyi makul bir \u015fekilde a\u011f\u0131rl\u0131kland\u0131rarak kapsaml\u0131 bir endeks elde eder ve kapsaml\u0131 de\u011ferlendirme i\u00e7in idealle\u015ftirilmi\u015f hedeften uzakl\u0131k derecesini \u00f6l\u00e7\u00fct olarak kullan\u0131r. Pamuk \u00e7i\u00e7eklerinin i\u00e7 kalite de\u011ferlendirmesinde \u00f6znel fakt\u00f6rlerin etkisini etkili bir \u015fekilde \u00f6nleyebilir ve belirli bir do\u011fruluk ve bilimsellik derecesine sahiptir. Bu \u00e7al\u0131\u015fmada, kapok \u00e7i\u00e7e\u011fi t\u0131bbi materyallerinin farkl\u0131 k\u0131s\u0131mlar\u0131n\u0131n (ta\u00e7 yapraklar, \u00e7anak yapraklar, stamenler) HPLC parmak izi spektrumlar\u0131 olu\u015fturulmu\u015ftur. Kapok \u00e7i\u00e7e\u011finin farkl\u0131 k\u0131s\u0131mlar\u0131n\u0131n parmak izi spektrumlar\u0131n\u0131n kalitesini de\u011ferlendirmek i\u00e7in benzerlik de\u011ferlendirmesi, \u0131s\u0131 haritas\u0131 k\u00fcmeleme analizi, temel bile\u015fen analizi ve ortogonal k\u0131smi en k\u00fc\u00e7\u00fck kareler diskriminant analizi kullan\u0131lm\u0131\u015ft\u0131r. Entropi a\u011f\u0131rl\u0131kl\u0131 TOPSIS y\u00f6ntemi, kapok \u00e7i\u00e7e\u011finin farkl\u0131 k\u0131s\u0131mlar\u0131n\u0131n kalitesini kapsaml\u0131 bir \u015fekilde puanlamak ve s\u0131ralamak i\u00e7in kullan\u0131lm\u0131\u015ft\u0131r. Ama\u00e7, kapok \u00e7i\u00e7e\u011fi t\u0131bbi malzemelerindeki \u00e7oklu g\u00f6sterge bile\u015fenlerinin zenginle\u015ftirilmesi ve kaynak geli\u015ftirme i\u00e7in veri deste\u011fi sa\u011flamak, kalite kontrol\u00fc ve klinik uygulama ara\u015ft\u0131rmalar\u0131 i\u00e7in temel olu\u015fturmakt\u0131r.<\/p>\n
<\/p>\n
Kromatografik ko\u015fullar incelendi\u011finde, bu \u00e7al\u0131\u015fma, tespit dalga boyu 300nm oldu\u011funda, baz\u0131 flavonoid bile\u015fiklerin kromatografik zirvelerinde kar\u015f\u0131l\u0131k gelen UV emilimi sergilemedi\u011fini bulmu\u015ftur; Tespit dalga boyu 255nm oldu\u011funda, her renk spektrumunun pik yan\u0131t de\u011ferlerinde \u00f6nemli bir fark vard\u0131r. Mangiferinin kromatografik pik yan\u0131t\u0131 daha y\u00fcksektir ve baz\u0131 kromatografik piklerde daha k\u00fc\u00e7\u00fck emilim veya kay\u0131p vard\u0131r; Tespit dalga boyu 230nm oldu\u011funda, her bir spektral pik sinyalinin yan\u0131t de\u011ferleri nispeten y\u00fcksektir ve taban \u00e7izgisi nispeten sabittir. Bu nedenle, 230nm alg\u0131lama dalga boyu olarak kullan\u0131l\u0131r. Ayn\u0131 zamanda, farkl\u0131 ekstraksiyon y\u00f6ntemleri (ultrason, refl\u00fc), ekstraksiyon \u00e7\u00f6z\u00fcc\u00fcleri (metanol, 50% metanol, 70% metanol), \u00e7\u00f6z\u00fcc\u00fc dozaj\u0131 (15, 25, 50 mL) ve ekstraksiyon s\u00fcresi (30, 45, 60 dakika) de ara\u015ft\u0131r\u0131lm\u0131\u015ft\u0131r. Sonu\u00e7lar, \"2.3\" alt\u0131ndaki y\u00f6ntem kullan\u0131larak haz\u0131rlanan numune \u00e7\u00f6zeltisinin daha iyi pik ayr\u0131m\u0131 ve daha y\u00fcksek bile\u015fen i\u00e7eri\u011fine sahip oldu\u011funu g\u00f6stermi\u015ftir.
\nAra\u015ft\u0131rma sonu\u00e7lar\u0131, pamuk \u00e7i\u00e7ekleri ve \u00e7anak yapraklar aras\u0131ndaki benzerli\u011fin nispeten y\u00fcksek oldu\u011funu, ta\u00e7 yapraklar ve organlar aras\u0131ndaki benzerli\u011fin ise nispeten yak\u0131n oldu\u011funu g\u00f6stermektedir. Is\u0131 haritas\u0131 k\u00fcmeleme analizi sonu\u00e7lar\u0131, ta\u00e7 yapraklar ve stamenler aras\u0131ndaki kalite tutarl\u0131l\u0131\u011f\u0131n\u0131n daha y\u00fcksek oldu\u011funu ve pamuk \u00e7i\u00e7eklerinin kimyasal bile\u015fenlerinin esas olarak \u00e7anak yapraklarda zenginle\u015fti\u011fini g\u00f6stermektedir ki bu da benzerlik sonu\u00e7lar\u0131yla tutarl\u0131d\u0131r. CA ve PCA sonu\u00e7lar\u0131, her ikisinin de \u00e7anak yapraklar, ta\u00e7 yapraklar ve stamen \u00f6rnekleri aras\u0131ndaki \u00f6nemli farkl\u0131l\u0131klar\u0131 a\u00e7\u0131k\u00e7a karakterize edebildi\u011fini, ta\u00e7 yapraklar ve stamenler aras\u0131ndaki farkl\u0131l\u0131klar\u0131n ise nispeten k\u00fc\u00e7\u00fck oldu\u011funu g\u00f6stermektedir. OPLS-DA analizi ile birle\u015ftirildi\u011finde, pamuk \u00e7i\u00e7eklerinin ta\u00e7 yapraklar\u0131n\u0131, \u00e7anak yapraklar\u0131n\u0131 ve stamenlerini tamamen ay\u0131rt etmek m\u00fcmk\u00fcnd\u00fcr. Pik 8, mangiferin, pik 13, pik 6, klorojenik asit, pik 5 ve rutin, pamuk \u00e7i\u00e7e\u011fi t\u0131bbi malzemelerinin farkl\u0131 k\u0131s\u0131mlar\u0131n\u0131 ay\u0131rt etmek i\u00e7in g\u00f6sterge bile\u015fenleri olarak kullan\u0131labilen \u00fc\u00e7\u00fc aras\u0131ndaki diferansiyel bile\u015fiklerdir. Ayn\u0131 zamanda, entropi a\u011f\u0131rl\u0131\u011f\u0131 TOPSIS y\u00f6ntemi ile birlikte, 15 parti kapok \u00e7i\u00e7e\u011fi t\u0131bbi malzemesinin farkl\u0131 k\u0131s\u0131mlar\u0131n\u0131n karakteristik zirveleri a\u011f\u0131rl\u0131kland\u0131r\u0131ld\u0131 ve kapok \u00e7i\u00e7e\u011fi t\u0131bbi malzemelerinin farkl\u0131 k\u0131s\u0131mlar\u0131n\u0131n \u00f6rnekleri, kapok \u00e7i\u00e7e\u011fi t\u0131bbi malzemelerinin genel kontrol\u00fcn\u00fc ve y\u00fcksek kaliteli tohum kaynaklar\u0131n\u0131n taranmas\u0131n\u0131 sa\u011flayabilen Ci de\u011ferine g\u00f6re s\u0131raland\u0131. Ara\u015ft\u0131rma sonu\u00e7lar\u0131, Yunnan Eyaletindeki pamuk \u00e7i\u00e7e\u011fi \u00f6rneklerinin ortalama Ci de\u011ferinin di\u011fer \u00fcretim alanlar\u0131ndaki \u00f6rneklerden daha y\u00fcksek oldu\u011funu ve \u00e7anak yaprak kalitesinin daha iyi oldu\u011funu g\u00f6stermektedir.
\n\u00d6zetle, bu \u00e7al\u0131\u015fmada kapok \u00e7i\u00e7e\u011fi t\u0131bbi malzemeleri ve bunlar\u0131n farkl\u0131 k\u0131s\u0131mlar\u0131 i\u00e7in olu\u015fturulan HPLC parmak izi tespit y\u00f6ntemi kararl\u0131 ve g\u00fcvenilirdir. Kimyasal \u00f6r\u00fcnt\u00fc tan\u0131ma ve entropi a\u011f\u0131rl\u0131\u011f\u0131 TOPSIS y\u00f6ntemi sayesinde, kapok \u00e7i\u00e7e\u011fi t\u0131bbi malzemelerinin ve farkl\u0131 k\u0131s\u0131mlar\u0131n\u0131n HPLC parmak izi kapsaml\u0131, kapsaml\u0131 ve sistematik olarak analiz edilebilir ve de\u011ferlendirilebilir. Bu, numunelerin kalite de\u011ferlendirmesi ve diferansiyel analizine, farkl\u0131 k\u0131s\u0131mlardaki kimyasal bile\u015fim farkl\u0131l\u0131klar\u0131n\u0131n kar\u015f\u0131la\u015ft\u0131r\u0131lmas\u0131na, kimyasal bile\u015fenlerin da\u011f\u0131l\u0131m modelinin netle\u015ftirilmesine ve kapok \u00e7i\u00e7e\u011fi t\u0131bbi malzemelerinin kalite kontrol\u00fc ve klinik uygulamas\u0131 i\u00e7in veri deste\u011fine olanak tan\u0131r.<\/p>","protected":false},"excerpt":{"rendered":"
Analysis of Differences in Different Parts of Cotton Flowers Based on Chemical Pattern Recognition and Entropy Weight TOPSIS Method Cotton flowers are the dry flowers of the cotton plant Gossampinus malabarica (DC.) Merr. They are cool in nature and belong to the large intestine meridian. They have the effects of clearing heat and dampness, detoxifying, […]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"\n