Tartary Karabu\u011fday Polipeptitlerinin Safla\u015ft\u0131r\u0131lmas\u0131, Yap\u0131s\u0131 ve \u0130n vitro Antioksidan Aktivitesi
\nKara bu\u011fday, karabu\u011fday, \u00fc\u00e7gen bu\u011fday vb. olarak da bilinen karabu\u011fday, Polygonaceae familyas\u0131na ve Karabu\u011fday cinsine aittir. \u00c7in k\u00f6kenli dikotiledon bir bitkidir ve d\u00fcnyadaki en eski ekili \u00fcr\u00fcnlerden biridir. Ayn\u0131 zamanda \u00c7in'de g\u0131da ve ilac\u0131 birle\u015ftiren e\u015fsiz bir k\u00fc\u00e7\u00fck tah\u0131l \u00fcr\u00fcn\u00fcd\u00fcr. Compendium of Materia Medica'ya g\u00f6re, ac\u0131 karabu\u011fday ac\u0131, hafif ve so\u011fuk bir tada sahiptir ve qi'yi azaltma, ba\u011f\u0131rsaklar\u0131 geni\u015fletme, ruhu s\u00fcrd\u00fcrme, qi dola\u015f\u0131m\u0131n\u0131 te\u015fvik etme ve kan staz\u0131n\u0131 giderme i\u015flevlerine sahiptir. \u00d6nceki \u00e7al\u0131\u015fmalar, ac\u0131 karabu\u011fday tanelerinden elde edilen ham peptit \u00f6zlerinin ve bunlar\u0131n protein hidrolizinin kolesterol d\u00fc\u015f\u00fcrme, kanser ve t\u00fcm\u00f6r \u00f6nleme, hiperlipidemi \u00f6nleme ve antioksidan etkiler gibi \u00e7e\u015fitli biyolojik aktivitelere sahip oldu\u011funu g\u00f6stermi\u015ftir.
\nBiyoaktif peptitler, amino asit bile\u015fimleri ve dizilimleriyle yak\u0131ndan ili\u015fkili olan \u00e7ok say\u0131da biyolojik i\u015fleve sahiptir. Bu nedenle, peptitlerin etkinli\u011fini incelemenin anahtar\u0131 yap\u0131lar\u0131n\u0131 tan\u0131mlamakt\u0131r. Ay\u0131rma ve safla\u015ft\u0131rma, peptit yap\u0131s\u0131 ve etkinli\u011fi aras\u0131ndaki ili\u015fkinin incelenmesinde \u00e7ok \u00f6nemli bir ad\u0131md\u0131r. Yayg\u0131n olarak kullan\u0131lan peptit ay\u0131rma ve safla\u015ft\u0131rma teknikleri aras\u0131nda kromatografi, membran ay\u0131rma, elektroforez vb. yer al\u0131r. Bunlar aras\u0131nda kromatografi teknolojisi en yayg\u0131n kullan\u0131lan\u0131d\u0131r. Protein hidrolizatlar\u0131n\u0131n karma\u015f\u0131k bile\u015fimi nedeniyle, peptit ay\u0131rma ve zenginle\u015ftirme elde etmek i\u00e7in ay\u0131rma ve safla\u015ft\u0131rma i\u00e7in genellikle birden fazla y\u00f6ntem birlikte kullan\u0131l\u0131r. \u00d6nceki \u00e7al\u0131\u015fmalar, tartar karabu\u011fday proteini veya enzimatik hidroliz \u00fcr\u00fcnlerinin \u00e7e\u015fitli biyolojik aktivitelere sahip oldu\u011funu g\u00f6stermi\u015ftir, ancak biyolojik aktivite mekanizmalar\u0131 hen\u00fcz \u00e7ok net de\u011fildir ve tartar karabu\u011fday aktif peptitlerinin yap\u0131sal analizi ve biyolojik aktivitesi hakk\u0131nda \u00e7ok az rapor vard\u0131r. Ma ve arkada\u015flar\u0131, sindirilmi\u015f karabu\u011fday proteininden, ABTS serbest radikallerini karabu\u011fday protein kar\u0131\u015f\u0131mlar\u0131ndan daha g\u00fc\u00e7l\u00fc bir \u015fekilde temizleme yetene\u011fine sahip d\u00f6rt antioksidan peptidi (WPL, VPW, VFPW ve PW) izole etmi\u015f ve tan\u0131mlam\u0131\u015ft\u0131r. Luo, tartar karabu\u011fday protein hidrolizat\u0131 elde etmek amac\u0131yla tartar karabu\u011fday proteinini hidrolize etmek i\u00e7in alkali proteaz kullanm\u0131\u015ft\u0131r. Ultrafiltrasyon ay\u0131rma, anyon de\u011fi\u015fim kromatografisi ve ters y\u00fcksek performansl\u0131 s\u0131v\u0131 kromatografisinden sonra, tartary karabu\u011fday\u0131n aktif peptit bile\u015feni ba\u015far\u0131yla ayr\u0131ld\u0131 ve amino asit dizisi Ala Phe Tyr Arg Trp olarak elde edildi.
\nBu \u00e7al\u0131\u015fmada, tartar karabu\u011fday\u0131n ham proteinini \u00e7\u0131karmak i\u00e7in tartar karabu\u011fday\u0131n fonksiyonel ekstrakt\u0131n\u0131n at\u0131k kal\u0131nt\u0131s\u0131 hammadde olarak kullan\u0131lm\u0131\u015f ve bitki lactobacillus ile fermantasyon yoluyla tartar karabu\u011fday peptitleri haz\u0131rlanm\u0131\u015ft\u0131r. Elde edilen tartar karabu\u011fday polipeptit fermantasyon suyu makro g\u00f6zenekli adsorpsiyon re\u00e7inesi, dekstran jel ve s\u0131v\u0131 kromatografi ile safla\u015ft\u0131r\u0131lm\u0131\u015f ve antioksidan aktivitesi belirlenmi\u015ftir. G\u00fc\u00e7l\u00fc antioksidan aktiviteye sahip se\u00e7ilen k\u0131s\u0131mlar\u0131n yap\u0131s\u0131 tan\u0131mlanm\u0131\u015f ve tartar karabu\u011fday polipeptidinin daha fazla ara\u015ft\u0131r\u0131lmas\u0131 ve fonksiyonel g\u0131da ve sa\u011fl\u0131k \u00fcr\u00fcnlerinde uygulanmas\u0131 i\u00e7in teorik bir temel sa\u011flamak amac\u0131yla tartar karabu\u011fday polipeptidinin ham ekstrakt\u0131n\u0131n antioksidan aktivitesinin ana kayna\u011f\u0131 ara\u015ft\u0131r\u0131lm\u0131\u015ft\u0131r.<\/p>\n
<\/p>\n
Sephadex G-15 glukan jel ay\u0131rma ve safla\u015ft\u0131rma i\u015fleminden sonra, T-3 bile\u015feninin ABTS ve DPPH serbest radikal s\u00fcp\u00fcrme kapasitesi en iyisiydi ve s\u00fcp\u00fcrme oranlar\u0131 s\u0131ras\u0131yla 96% ve 94% idi. Makrog\u00f6zenekli adsorpsiyon re\u00e7inesi ile safla\u015ft\u0131r\u0131lan tartar karabu\u011fday polipeptidi, jel ile ayr\u0131lan ve safla\u015ft\u0131r\u0131lan T-3 bile\u015fenine benzer \u015fekilde DPPH ve ABTS \u00fczerinde g\u00fc\u00e7l\u00fc bir inhibit\u00f6r etkiye sahiptir ve \u00f6nemli antioksidan aktivite g\u00f6sterir.
\nS\u0131v\u0131 kromatografisi ile ayr\u0131lan T-3-1 bile\u015feni \u00fczerinde materyal tan\u0131mlamas\u0131 ger\u00e7ekle\u015ftirilmi\u015f ve T-3-1 bile\u015feninden toplam 249 protein ve 2324 peptit tan\u0131mlanm\u0131\u015ft\u0131r. Tan\u0131mlanan peptitler \u00e7o\u011funlukla 10-15 amino asitten olu\u015fmaktad\u0131r ve biyolojik olarak aktif peptitlerin uzunluk aral\u0131\u011f\u0131na aittir. T-3-1 bile\u015feni k\u00fctle spektrometresi tan\u0131mlama sonu\u00e7lar\u0131na g\u00f6re, ana antioksidan peptitlerin amino asit dizileri fenilalanin prolin tirozin (Phe Pro Tyr, FPY) ve tirozin l\u00f6sin prolin fenilalanindir (Tyr Leu Pro Phe, YLPF).
\nTartar karabu\u011fday peptitlerinin antioksidan kapasitesi farkl\u0131 safl\u0131klara g\u00f6re de\u011fi\u015fmektedir. T-3 bile\u015feninin s\u0131v\u0131 kromatografisi ile daha fazla ayr\u0131lmas\u0131 ve safla\u015ft\u0131r\u0131lmas\u0131ndan sonra, T-3-1 bile\u015feni elde edilmi\u015ftir. Tartar karabu\u011fday peptitlerinin safl\u0131\u011f\u0131 98%'ye ula\u015fmas\u0131na ra\u011fmen, DP-PH ve ABTS'nin inhibisyon oranlar\u0131 art\u0131k artmam\u0131\u015f, ancak s\u0131ras\u0131yla 89% ve 90% inhibisyon oranlar\u0131yla hafif\u00e7e azalm\u0131\u015ft\u0131r. Fermantasyon y\u00f6ntemiyle haz\u0131rlanan tartar karabu\u011fday peptitlerinin antioksidan aktivitesi peptit safl\u0131\u011f\u0131yla tam olarak pozitif korelasyon g\u00f6stermeyebilir. Safl\u0131k belirli bir dereceye kadar art\u0131r\u0131ld\u0131\u011f\u0131nda, peptitlerin antioksidan aktivitesi asl\u0131nda azal\u0131r<\/p>","protected":false},"excerpt":{"rendered":"
Purification, Structure, and In vitro Antioxidant Activity of Tartary Buckwheat Polypeptides Buckwheat, also known as black wheat, buckwheat, triangle wheat, etc., belongs to the family Polygonaceae and the genus Buckwheat. It is a dicotyledonous plant that originated in China and is one of the earliest cultivated crops in the world. It is also a unique […]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"\n