\u00dc\u00e7 Tujia bitkisinin \u00fclseratif kolitli farelerin ba\u011f\u0131rsak mikrobiyotas\u0131 \u00fczerindeki etkisi
\n\u00dclseratif kolit (\u00dcK), klinik olarak kar\u0131n a\u011fr\u0131s\u0131, ishal, mukus, irin, kanl\u0131 d\u0131\u015fk\u0131 ve di\u011fer semptomlarla karakterize, spesifik olmayan kronik bir enflamatuar hastal\u0131kt\u0131r. \u00dclseratif kolitin geleneksel \u00c7in t\u0131bb\u0131 sendrom tipleri temel olarak kal\u0131n ba\u011f\u0131rsakta nemli \u0131s\u0131, karaci\u011fer depresyonu ve dalak eksikli\u011fi, yin eksikli\u011fi ve ba\u011f\u0131rsak kurulu\u011fu, dalak ve mide zay\u0131fl\u0131\u011f\u0131, dalak ve b\u00f6brek yang eksikli\u011fi, kan staz\u0131 ve ba\u011f\u0131rsak kollateralleri ve kar\u0131\u015f\u0131k so\u011fuk ve s\u0131ca\u011f\u0131 i\u00e7erir. Bir\u00e7ok t\u0131p pratisyeni \"sendroma g\u00f6re mevzuat ve yasaya g\u00f6re re\u00e7ete\" tedavi ilkesini takip eder ve tedavi i\u00e7in genellikle \u0131s\u0131y\u0131 temizleyen, nemi art\u0131ran, qi'yi besleyen ve dala\u011f\u0131 canland\u0131ran \u00fcr\u00fcnler kullan\u0131r. \u00dc\u00e7 i\u011fne (Berberidis Radix), istiridye domuzu dikeni Berberis julianae Schneid, k\u00fc\u00e7\u00fck coptis dikeni Berberis wilsonae Hemsl, ince yaprak Berberis poiretii Schneid veya ka\u015f\u0131k yapra\u011f\u0131 Berberis vernae Schneid gibi Berberidaceae familyas\u0131na ait \u00e7e\u015fitli bitki t\u00fcrlerinin kurutulmu\u015f k\u00f6kleridir. Do\u011falar\u0131 gere\u011fi so\u011fuk, tatlar\u0131 ac\u0131d\u0131r ve karaci\u011fer, mide ve kal\u0131n ba\u011f\u0131rsak meridyenlerine aittirler. Ate\u015fi azaltma, nemi kurutma, \u0131s\u0131y\u0131 temizleme ve detoksifiye etme etkilerine sahiptirler. Enshi Tujia ve Miao \u00d6zerk Vilayeti'nde otantik t\u0131bbi malzemelerdir. Tujia t\u0131bb\u0131, ishal ve dizanteriyi tedavi etmek i\u00e7in genellikle tek ba\u015f\u0131na veya Yingshan Honggen, Nar Kabu\u011fu, Wu Zhuyu vb. ile birlikte \u00fc\u00e7 i\u011fne kullan\u0131r, \u00f6nemli terap\u00f6tik etkileri vard\u0131r, ancak spesifik etki mekanizmas\u0131 hen\u00fcz net de\u011fildir.
\nBa\u011f\u0131rsak mikrobiyotas\u0131 bol ve \u00e7e\u015fitlidir, kona\u011f\u0131n ba\u011f\u0131\u015f\u0131kl\u0131k tepkisinde, metabolizmas\u0131nda ve hastal\u0131k ilerlemesinde \u00f6nemli bir rol oynar. Ba\u011f\u0131rsak mikrobiyotas\u0131, ba\u011f\u0131rsak mukozal bariyeri, madde emilimi, metabolizma ve beslenme i\u015flevlerine kat\u0131l\u0131rken, T lenfositlerinin \u00e7o\u011falmas\u0131na ve farkl\u0131la\u015fmas\u0131na arac\u0131l\u0131k eder ve ba\u011f\u0131rsak imm\u00fcn yan\u0131t\u0131n\u0131 d\u00fczenler. \u00c7ok say\u0131da \u00e7al\u0131\u015fma ba\u011f\u0131rsak mikrobiyotas\u0131n\u0131n disbiyozunun \u00fclseratif kolit, irritabl ba\u011f\u0131rsak sendromu ve kolorektal kanser ile yak\u0131ndan ili\u015fkili oldu\u011funu g\u00f6stermi\u015ftir. G\u00fcn\u00fcm\u00fczde, aminosalisilik asit ila\u00e7lar\u0131, steroid ila\u00e7lar, imm\u00fcnosupresanlar veya cerrahi, \u00fclseratif kolit tedavisinde yayg\u0131n olarak kullan\u0131lmakta olup, ortalama etkinlik ve kolay n\u00fcks s\u00f6z konusudur. Son y\u0131llarda, ilgili literat\u00fcr geleneksel \u00c7in t\u0131bb\u0131n\u0131n ba\u011f\u0131rsak mikrobiyotas\u0131n\u0131n yap\u0131s\u0131n\u0131 d\u00fczenleyerek ve mikrobiyota \u00e7e\u015fitlili\u011fini geri kazand\u0131rarak \u00fclseratif koliti etkili bir \u015fekilde tedavi edebilece\u011fini bildirmi\u015ftir. Buna dayanarak, ba\u011f\u0131rsak mikrobiyotas\u0131 arac\u0131l\u0131\u011f\u0131 perspektifinden etki mekanizmalar\u0131n\u0131 \u00f6nceden ke\u015ffetmek i\u00e7in \u00fc\u00e7 ilac\u0131n \u00fclseratif kolitli farelerin ba\u011f\u0131rsak mikrobiyotas\u0131 \u00fczerindeki etkilerini ara\u015ft\u0131rmay\u0131 planl\u0131yoruz.<\/p>\n
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Bu deney ilk olarak \u00fc\u00e7 ilac\u0131n UC fareleri \u00fczerindeki m\u00fcdahale etkilerini a\u011f\u0131rl\u0131k de\u011fi\u015fiklikleri, DAI skorlar\u0131, kolon uzunlu\u011fu ve doku morfolojisi a\u00e7\u0131s\u0131ndan do\u011frulam\u0131\u015ft\u0131r. \u0130kinci olarak, \u00fc\u00e7 ilac\u0131n UC farelerinin ba\u011f\u0131rsak mikrobiyotas\u0131 \u00fczerindeki etkileri incelenmi\u015ftir. Sonu\u00e7lar, \u00fc\u00e7 i\u011fnenin farelerin a\u011f\u0131rl\u0131\u011f\u0131n\u0131, DAI skorunu ve kolon uzunlu\u011funu \u00f6nemli \u00f6l\u00e7\u00fcde iyile\u015ftirdi\u011fini, inflamatuar infiltrasyonu azaltt\u0131\u011f\u0131n\u0131 ve ba\u011f\u0131rsak mukozal hasar\u0131n\u0131 onard\u0131\u011f\u0131n\u0131 g\u00f6stermi\u015ftir. S\u0131ralama sonu\u00e7lar\u0131, \u00fc\u00e7 i\u011fnenin DSS taraf\u0131ndan ind\u00fcklenen bozulmu\u015f mikrobiyota yap\u0131s\u0131n\u0131 \u00f6nemli \u00f6l\u00e7\u00fcde eski haline getirebildi\u011fini g\u00f6stermi\u015f ve \u00fc\u00e7 i\u011fnenin UC \u00fczerindeki m\u00fcdahale etkisinin ba\u011f\u0131rsak mikrobiyotas\u0131 ile y\u00fcksek oranda ili\u015fkili olabilece\u011fini d\u00fc\u015f\u00fcnd\u00fcrm\u00fc\u015ft\u00fcr. Normal grup, model grup ve \u00fc\u00e7 i\u011fne grubundaki ba\u011f\u0131rsak mikrobiyotas\u0131n\u0131n Pcoa ve k\u00fcme analizinde, model grup ve normal grup fareler aras\u0131nda ba\u011f\u0131rsak mikrobiyota yap\u0131s\u0131nda \u00f6nemli farkl\u0131l\u0131klar oldu\u011fu bulunmu\u015ftur. Bununla birlikte, \u00fc\u00e7 i\u011fne ile m\u00fcdahaleden sonra, ba\u011f\u0131rsak mikrobiyotas\u0131 \u00f6nemli \u00f6l\u00e7\u00fcde de\u011fi\u015fmi\u015f ve topluluk yap\u0131s\u0131 analizi ve LEfSe analizi ile do\u011frulanan normal gruba yakla\u015fm\u0131\u015ft\u0131r. Gen fonksiyonu tahmini yoluyla, UC'de \u00fc\u00e7 i\u011fnenin m\u00fcdahalesinin ba\u011f\u0131rsak mikrobiyotas\u0131n\u0131n metabolik fonksiyonuyla da ilgili olabilece\u011fi ve sonraki ara\u015ft\u0131rmalar i\u00e7in yeni bir y\u00f6n sa\u011flad\u0131\u011f\u0131 bulunmu\u015ftur.
\nBa\u011f\u0131rsak mikrobiyotas\u0131, ba\u011f\u0131rsak mukozal bariyerinde biyolojik bir bariyer olarak, v\u00fccutta sa\u011fl\u0131k ve hastal\u0131k aras\u0131ndaki dengenin korunmas\u0131 i\u00e7in \u00e7ok \u00f6nemlidir ve beyin ba\u011f\u0131rsak ekseninin bir par\u00e7as\u0131d\u0131r. Ba\u011f\u0131rsak mikrobiyotas\u0131n\u0131n dengesizli\u011fi, zararl\u0131 bakterilerin \u00e7o\u011falmas\u0131na ve yararl\u0131 bakterilerin azalmas\u0131na yol a\u00e7arak ba\u011f\u0131rsak mikrobiyotas\u0131n\u0131n yap\u0131sal bozuklu\u011funa ve \u00e7e\u015fitlili\u011finin azalmas\u0131na, dolay\u0131s\u0131yla ba\u011f\u0131rsak mukozas\u0131nda hasara neden olabilir. Baz\u0131 \u00e7al\u0131\u015fmalar, ba\u011f\u0131rsak mikrobiyota dengesizli\u011finin arac\u0131l\u0131k etti\u011fi anormal imm\u00fcn inflamatuar yan\u0131t\u0131n \u00dcK'nin \u00f6nemli nedenlerinden biri oldu\u011funu \u00f6ne s\u00fcrmektedir. Bu nedenle, mikrobiyal preparatlar yoluyla ba\u011f\u0131rsak mikrobiyotas\u0131n\u0131n homeostaz\u0131n\u0131n yeniden sa\u011flanmas\u0131, \u00dcK tedavisi i\u00e7in giderek yeni bir s\u0131cak nokta haline gelmi\u015ftir. Geleneksel \u00c7in t\u0131bb\u0131 uygulamas\u0131n\u0131n ana yolu, ka\u00e7\u0131n\u0131lmaz olarak v\u00fccuttaki ba\u011f\u0131rsak mikrobiyotas\u0131 ile temas eden ve \u00e7ift y\u00f6nl\u00fc bir d\u00fczenleyici etki \u00fcreten oral kaynatmalard\u0131r. Baz\u0131 geleneksel \u00c7in ila\u00e7lar\u0131, ba\u011f\u0131rsak mikrobiyotas\u0131n\u0131n yap\u0131s\u0131n\u0131 ve \u00e7e\u015fitlili\u011fini restore ederek ve homeostaz\u0131n\u0131 koruyarak terap\u00f6tik etkiler elde eder. Modern farmakolojik \u00e7al\u0131\u015fmalar, \u00fc\u00e7 i\u011fnenin esas olarak berberin, palmatin, jatrorrhizine ve palmatin gibi \u00e7e\u015fitli alkaloidler i\u00e7erdi\u011fini g\u00f6stermi\u015ftir. Ara\u015ft\u0131rmalar, berberinin Bacteroidetes'in bollu\u011funu art\u0131rabildi\u011fini ve Firmicutes'in bollu\u011funu azaltabildi\u011fini g\u00f6stermi\u015ftir. S\u0131\u00e7anlarda DSS kaynakl\u0131 \u00fclseratif koliti tedavi etmek i\u00e7in ba\u011f\u0131rsak mikrobiyotas\u0131yla ili\u015fkili triptofan metabolitleri arac\u0131l\u0131\u011f\u0131yla aril hidrokarbon resept\u00f6rlerini (AhR) aktive edebilir. Zhang ve arkada\u015flar\u0131, y\u00fcksek verimli dizileme sonu\u00e7lar\u0131yla, palmatinin Bacteroidetes'in g\u00f6receli bollu\u011funu art\u0131rd\u0131\u011f\u0131n\u0131, yararl\u0131 bakterileri art\u0131rd\u0131\u011f\u0131n\u0131, zararl\u0131 bakterileri azaltt\u0131\u011f\u0131n\u0131 ve bakteriyel \u00e7e\u015fitlili\u011fi geri kazand\u0131rarak ve triptofan bozulmas\u0131n\u0131 engelleyerek DSS kaynakl\u0131 \u00fclseratif koliti iyile\u015ftirdi\u011fini g\u00f6stermi\u015ftir. Yukar\u0131daki ara\u015ft\u0131rma raporlar\u0131 temelde bu deneyin sonu\u00e7lar\u0131yla tutarl\u0131d\u0131r. Ayn\u0131 zamanda, bu deneyin LEfSe analizinde, model grupla kar\u015f\u0131la\u015ft\u0131r\u0131ld\u0131\u011f\u0131nda, Lactobacillus tak\u0131m\u0131, Lactobacillus cinsi ve Lactobacillus ailesi, normal grup ve \u00fc\u00e7 i\u011fne grubu taraf\u0131ndan payla\u015f\u0131lan bask\u0131n bakteri t\u00fcrleriydi. Lactobacillus'un ishali iyile\u015ftirdi\u011fi kan\u0131tlanm\u0131\u015ft\u0131r. Ayr\u0131ca Lactobacillus, insan kolon epitel h\u00fccrelerinde sitokin kaynakl\u0131 apoptozu inhibe ederek ve m\u00fcsin-2 (MUC-2) i\u00e7eri\u011fini art\u0131rarak farelerin kolon mukozal bariyer fonksiyonunu iyile\u015ftirebilir. Clostridium, insan sa\u011fl\u0131\u011f\u0131 i\u00e7in tehdit olu\u015fturan ekzotoksinler salg\u0131layan zorunlu anaerobik bir bakteridir. Clostridium'daki art\u0131\u015f ba\u011f\u0131rsak hastal\u0131klar\u0131n\u0131n olu\u015fumunu te\u015fvik eder. \u00dc\u00e7 i\u011fneli bitki Coptis chinensis, clostridia'n\u0131n b\u00fcy\u00fcmesini engelleyebilir ve clostridia say\u0131s\u0131n\u0131 azaltarak ba\u011f\u0131rsak kanserini \u00f6nleyebilir. Bu deneyde, Clostridia cinsi, Clostridia s\u0131n\u0131f\u0131 ve Clostridia tak\u0131m\u0131 model grupta bask\u0131n bakteri t\u00fcrleri olurken, normal grup ve \u00fc\u00e7 i\u011fne grubu daha d\u00fc\u015f\u00fck bollu\u011fa sahipti ve bu da literat\u00fcr ara\u015ft\u0131rmas\u0131n\u0131n sonu\u00e7lar\u0131yla nispeten tutarl\u0131yd\u0131.<\/p>","protected":false},"excerpt":{"rendered":"
The effect of three Tujia herbs on the gut microbiota of mice with ulcerative colitis Ulcerative colitis (UC) is a chronic non-specific inflammatory disease, characterized clinically by abdominal pain, diarrhea, mucus, pus, bloody stools, and other symptoms. The traditional Chinese medicine syndrome types of ulcerative colitis mainly include damp heat in the large intestine, liver […]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"\n