UPLC-Q-TOF-MS Teknolojisi ve PLS-DA Analizine Dayal\u0131 Be\u015f \u0130lkel Artemisia annua'n\u0131n Kimyasal Bile\u015fim Farkl\u0131l\u0131klar\u0131 \u00dczerine \u00c7al\u0131\u015fma
\nTibet t\u0131bb\u0131, \u00c7in'deki d\u00f6rt b\u00fcy\u00fck etnik ila\u00e7 aras\u0131nda en \u00f6nde gelenidir ve e\u015fsiz co\u011frafi ortam\u0131 Tibet t\u0131bbi malzemeleri i\u00e7in benzersiz bir terap\u00f6tik etki yaratm\u0131\u015ft\u0131r. Yayg\u0131n olarak kullan\u0131lan bir Tibet ilac\u0131 olan Artemisia annua, \"Yeni G\u00f6zden Ge\u00e7irilmi\u015f Kristal \u0130nci Materia Medica\", \"Etnik T\u0131p S\u00f6zl\u00fc\u011f\u00fc\", \"Tibet T\u0131bb\u0131 Bakanl\u0131\u011f\u0131\", \"Ulusal Tibet T\u0131bb\u0131 Standard\u0131 Tam Kitab\u0131\", \"Tibet T\u0131bb\u0131 Kroni\u011fi\" gibi Tibet t\u0131bb\u0131 klasiklerinde kaydedilmi\u015ftir. Meconopsis Vig. 1814'te Viguier taraf\u0131ndan Papaver cambium ha\u015fha\u015f\u0131na dayal\u0131 olarak olu\u015fturulan olduk\u00e7a ay\u0131rt edici bir y\u00fcksek irtifa Tibet ilac\u0131d\u0131r. Do\u011fu Asya ve Bat\u0131 Avrupa'da aral\u0131kl\u0131 olarak da\u011f\u0131l\u0131m g\u00f6steren 2 alt cins, 5 grup ve 9 soya ayr\u0131l\u0131r. Bitki boylar\u0131 10 ila 180 cm aras\u0131nda de\u011fi\u015fen tek veya \u00e7ok y\u0131ll\u0131k bir bitkidir. Farkl\u0131 t\u00fcrlerin farkl\u0131 \u00e7i\u00e7ek \u015fekilleri ve renkleri vard\u0131r ve Meconopsis'in bir\u00e7ok t\u00fcr\u00fc yumu\u015fak ve uzun t\u00fcylerle kapl\u0131 g\u00f6vde ve yapraklara sahiptir, dolay\u0131s\u0131yla ad\u0131 da buradan gelmektedir. Kuzey ve G\u00fcney aras\u0131ndaki t\u0131bbi kullan\u0131m farkl\u0131l\u0131klar\u0131 nedeniyle, farkl\u0131 b\u00f6lgelerdeki Artemisia bitkilerinin kullan\u0131m\u0131 ve isimlerinde benzerlikler ve farkl\u0131l\u0131klar vard\u0131r, bu da ila\u00e7lar\u0131n kar\u0131\u015ft\u0131r\u0131lmas\u0131na, \u00e7e\u015fitlerin kar\u0131\u015ft\u0131r\u0131lmas\u0131na ve karma\u015f\u0131k k\u00f6kenlere neden olur. Bu nedenle, Artemisia t\u0131bbi bitkilerinin ara\u015ft\u0131r\u0131lmas\u0131 ve uygulanmas\u0131 zorluklarla kar\u015f\u0131 kar\u015f\u0131yad\u0131r.
\nUltra y\u00fcksek s\u0131v\u0131 kromatografisi d\u00f6rt kutuplu u\u00e7u\u015f zaman\u0131 tandem k\u00fctle spektrometresi (UPLC-Q-TOF-MS) y\u00fcksek \u00e7\u00f6z\u00fcn\u00fcrl\u00fck, hassasiyet ve ay\u0131rma kabiliyetine sahiptir ve referans standartlar\u0131n yoklu\u011funda karma\u015f\u0131k geleneksel \u00c7in t\u0131bb\u0131 bile\u015fenlerinin \u00f6n kalitatif analizini ger\u00e7ekle\u015ftirebilir. Do\u011fal ila\u00e7 analizi i\u00e7in yayg\u0131n olarak kullan\u0131lmaktad\u0131r. Bu \u00e7al\u0131\u015fmada, Meconopsis racemose Maxim Meconopsis horridula Hook f\uff06Thoms\u3001 Meconopsis quintubulina Regel, Meconopsis integrifolia (Maxim) etkilerini ara\u015ft\u0131rmak i\u00e7in UPLC-Q-TOF-MS kullan\u0131lm\u0131\u015ft\u0131r. Frans\u0131z ve aspirden 5 primordiadan olu\u015fan 49 parti Meconopsis punicea Maxim'in kimyasal bile\u015fimi tan\u0131mlanm\u0131\u015ft\u0131r. Temel bile\u015fen analizi (PCA) ve k\u0131smi en k\u00fc\u00e7\u00fck kareler diskriminant analizi (PLS-DA) matematiksel modelleri, Meconopsis punicea Maxim'in farkl\u0131 primordialar\u0131n\u0131n bile\u015fimindeki farkl\u0131l\u0131klar\u0131 ara\u015ft\u0131rmak i\u00e7in kullan\u0131lm\u0131\u015ft\u0131r. Bu, Meconopsis punicea \u00e7e\u015fitlerinin s\u0131n\u0131fland\u0131r\u0131lmas\u0131, materyal temeli ve rasyonel uygulamas\u0131 i\u00e7in bilimsel bir temel sa\u011flar.<\/p>\n
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\"\u00c7in Halk Cumhuriyeti Sa\u011fl\u0131k Bakanl\u0131\u011f\u0131 \u0130la\u00e7 Standartlar\u0131 (Tibet T\u0131bb\u0131) \"n\u0131n 1995 bask\u0131s\u0131nda, iki \u00e7e\u015fit dikenli ye\u015fil kadife artemisya ve ye\u015fil kadife artemisya ayr\u0131 ayr\u0131 yer alm\u0131\u015ft\u0131r. Sadece bir dikenli ye\u015fil kadife artemisya kayna\u011f\u0131 ve \u00fc\u00e7 ye\u015fil kadife artemisya kayna\u011f\u0131 vard\u0131r: b\u00fct\u00fcn yaprakl\u0131 ye\u015fil kadife artemisya, be\u015f damarl\u0131 ye\u015fil kadife artemisya ve uzun yaprakl\u0131 ye\u015fil kadife artemisya. Ancak, pazar ara\u015ft\u0131rmas\u0131 Tibet b\u00f6lgelerinde pratik uygulamalarda \u00e7ok az say\u0131da uzun yaprakl\u0131 ye\u015fil kadife artemisia \u00e7e\u015fidi oldu\u011funu ve kaynaklar\u0131n nispeten k\u0131t oldu\u011funu ortaya koymu\u015ftur. Raporlara g\u00f6re, sert b\u00fcy\u00fcme ortamlar\u0131 ve a\u015f\u0131r\u0131 insan s\u00f6m\u00fcr\u00fcs\u00fc nedeniyle Artemisia t\u00fcrlerinin kaynaklar\u0131 s\u0131n\u0131rl\u0131d\u0131r ve baz\u0131 Artemisia t\u00fcrleri \u00c7in'de korunan bitkiler olarak zaten tehlike alt\u0131ndad\u0131r. Bu nedenle, Tibet ilac\u0131 Artemisia annua \u00fczerine derinlemesine ara\u015ft\u0131rma ve \u00e7e\u015fitlerinin s\u0131n\u0131fland\u0131r\u0131lmas\u0131, Artemisia annua'n\u0131n rasyonel geli\u015fimi ve uygulanmas\u0131nda \u00f6nemli bir rol oynamaktad\u0131r.<\/p>\n
\u0130la\u00e7lar\u0131n farkl\u0131 klinik etkileri b\u00fcy\u00fck \u00f6l\u00e7\u00fcde kimyasal bile\u015fimlerindeki farkl\u0131l\u0131klardan kaynaklanmaktad\u0131r. Literat\u00fcr raporlar\u0131na g\u00f6re, Artemisia cinsindeki bitkiler alkaloidler, flavonoidler, u\u00e7ucu ya\u011flar, steroidler, terpenler vb. gibi kimyasal bile\u015fenler i\u00e7erir. Bunlar aras\u0131nda, alkaloidler ve flavonoidler ana aktif bile\u015fenlerdir ve son y\u0131llarda ara\u015ft\u0131rmalar\u0131n odak noktas\u0131 olmu\u015ftur. Baz\u0131 uzmanlar Artemisia annua kayna\u011f\u0131n\u0131n \"\u00c7in Halk Cumhuriyeti Sa\u011fl\u0131k Bakanl\u0131\u011f\u0131 \u0130la\u00e7 Standartlar\u0131'na (Tibet T\u0131bb\u0131)\" eklenmesini \u00f6nermi\u015flerdir, ancak bu \u00f6neriyi destekleyen herhangi bir ara\u015ft\u0131rma raporu bulunmamaktad\u0131r. Bu \u00e7al\u0131\u015fma, k\u00fctle spektrometresi kullanarak be\u015f ilkel Artemisia annua'n\u0131n kimyasal bile\u015fimini ara\u015ft\u0131ran ilk \u00e7al\u0131\u015fmad\u0131r. Bu \u00e7al\u0131\u015fmada 75 kimyasal bile\u015fenin tan\u0131mlanmas\u0131na dayanarak, Artemisia annua ve Artemisia annua aras\u0131nda 59 ortak kimyasal bile\u015fen vard\u0131r. Bu, Artemisia annua ve Artemisia annua'n\u0131n kimyasal bile\u015fiminin temelde ayn\u0131 oldu\u011funu ve di\u011fer \u00fc\u00e7 ilkel Artemisia annua'n\u0131n kimyasal bile\u015fiminden baz\u0131 farkl\u0131l\u0131klar oldu\u011funu g\u00f6stermektedir. Bu ara\u015ft\u0131rma sonucu, Artemisia annua'n\u0131n Artemisia annua'ya bir ilkel olarak eklenmesi i\u00e7in bilimsel bir temel sa\u011flayabilir.<\/p>\n
Maddi temel a\u00e7\u0131s\u0131ndan, be\u015f orijinal Artemisia annua aras\u0131ndaki kimyasal bile\u015fim farkl\u0131l\u0131klar\u0131, bu bitkinin t\u0131bbi etkinli\u011fi \u00fczerinde \u00f6nemli bir etkiye sahip olabilir. Dikenli Artemisia annua ve rasemik Artemisia annua'n\u0131n birlikte s\u0131n\u0131fland\u0131r\u0131lmas\u0131 ve rasemik Artemisia annua'n\u0131n dikenli Artemisia annua kayna\u011f\u0131na eklenmesi \u00f6nerilir; Wumai Ye\u015fil Kadife Artemisia, Quanyuan Ye\u015fil Kadife Artemisia ve Honghua Ye\u015fil Kadife Artemisia'n\u0131n her biri bir kategoride s\u0131n\u0131fland\u0131r\u0131l\u0131r; Pratik uygulamalarda, be\u015f darbeli ye\u015fil kadife artemisia, b\u00fct\u00fcn kenar ye\u015fil kadife artemisia, k\u0131rm\u0131z\u0131 \u00e7i\u00e7ek ye\u015fil kadife artemisia ve dikenli ye\u015fil kadife artemisia'n\u0131n kar\u0131\u015ft\u0131r\u0131lmas\u0131 tavsiye edilmez ve bunlar\u0131 ay\u0131rt etmek \u00f6nemlidir. \u015eu anda, Artemisia annua t\u00fcrleri karma\u015f\u0131kt\u0131r ve t\u00fcrlerde \u00f6nemli farkl\u0131l\u0131klar ile temel kaotiktir. \u00c7e\u015fit s\u0131n\u0131fland\u0131rmas\u0131na ve ara\u015ft\u0131rma uygulamalar\u0131na daha fazla dikkat edilmesi gerekmektedir. Bu \u00e7al\u0131\u015fma, be\u015f bazal Artemisia annua aras\u0131ndaki farkl\u0131l\u0131klar\u0131 materyal temeli a\u00e7\u0131s\u0131ndan analiz etmi\u015ftir ve diferansiyel bile\u015fenler, farkl\u0131 bazal Artemisia annua \u00e7e\u015fitlerinin tan\u0131mlanmas\u0131 i\u00e7in bir referans olarak kabul edilebilir. Daha sonra, be\u015f bazal Artemisia annua aras\u0131ndaki diferansiyel bile\u015fenlerin ayr\u0131nt\u0131l\u0131 bir analizi yap\u0131lacak ve bu be\u015f bazal Artemisia annua aras\u0131ndaki farkl\u0131l\u0131klar \u00fczerine farmakoloji ve klinik uygulamalar a\u00e7\u0131s\u0131ndan daha fazla ara\u015ft\u0131rma yap\u0131lacakt\u0131r.<\/p>","protected":false},"excerpt":{"rendered":"
Study on the Chemical Composition Differences of Five Primitive Artemisia annua Based on UPLC-Q-TOF-MS Technology and PLS-DA Analysis Tibetan medicine is the foremost among the four major ethnic medicines in China, and its unique geographical environment has created a unique therapeutic effect for Tibetan medicinal materials. As a commonly used Tibetan medicine, Artemisia annua is […]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"\n