{"id":7966,"date":"2024-08-11T09:57:23","date_gmt":"2024-08-11T09:57:23","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=7966"},"modified":"2024-08-11T09:57:23","modified_gmt":"2024-08-11T09:57:23","slug":"gougu-leaves","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/tr\/gougu-yapraklari\/","title":{"rendered":"Gougu Yapraklar\u0131ndaki Lipaz \u0130nhibe Edici Aktif Bile\u015fenlerin \u0130nce Tabaka Kromatografisi Biyootografi MSn Y\u00f6ntemi ile Karakterizasyonu"},"content":{"rendered":"<p>Gougu Yapraklar\u0131ndaki Lipaz \u0130nhibe Edici Aktif Bile\u015fenlerin \u0130nce Tabaka Kromatografisi Biyootografi MSn Y\u00f6ntemi ile Karakterizasyonu<br \/>\nIlex cornuta Lindl Ex Paxt, \u00c7in'de yayg\u0131n olarak bulunan Ilex familyas\u0131ndan Ilex cinsine ait bir bitkidir. Geleneksel bir \u00c7in ilac\u0131 olan Gougu'nun kurutulmu\u015f yapraklar\u0131, Tang Hanedanl\u0131\u011f\u0131'ndan bu yana bir\u00e7ok bitkisel monografide yer alm\u0131\u015ft\u0131r ve bin y\u0131l\u0131 a\u015fk\u0131n bir t\u0131bbi ge\u00e7mi\u015fe sahiptir. \u00c7in Halk Cumhuriyeti Farmakopesi'ne g\u00f6re (2020 bask\u0131s\u0131, Cilt 1), Ligusticum barbarum yapraklar\u0131n\u0131n tad\u0131 ac\u0131d\u0131r ve serin bir yap\u0131ya sahiptir. Karaci\u011fer ve b\u00f6brek meridyenlerine aittir ve \u0131s\u0131y\u0131 temizleme ve yin'i besleme, b\u00f6breklere fayda sa\u011flama ve karaci\u011feri sakinle\u015ftirme i\u015flevlerine sahiptir. T\u00fcberk\u00fcloz hemoptizi, kemik buharla\u015fmas\u0131 ve s\u0131cak basmas\u0131, ba\u015f d\u00f6nmesi ve vertigo vb. i\u00e7in kullan\u0131l\u0131r. Halk k\u00fclt\u00fcr\u00fcnde, Gougu yapraklar\u0131n\u0131n hararet giderici etkisinden dolay\u0131, yumu\u015fak yapraklar\u0131 Kuding \u00e7ay\u0131 olarak demlenip t\u00fcketilir. Modern ara\u015ft\u0131rmalar Cornus officinalis'in yapraklar\u0131n\u0131n triterpenoidler ve ana aktif bile\u015fenleri olan saponinler a\u00e7\u0131s\u0131ndan zengin oldu\u011funu g\u00f6stermi\u015ftir. Buna ek olarak, kan lipidlerini d\u00fc\u015f\u00fcrme, anti miyokardiyal iskemi, antioksidasyon, imm\u00fcnosupresyon ve anti fertilite gibi \u00e7e\u015fitli aktivitelere sahip flavonoidler, polifenoller, steroller vb. gibi \u00e7e\u015fitli bile\u015fenler vard\u0131r. \u00c7o\u011fu zay\u0131flama ilac\u0131n\u0131n \u00f6nemsiz etkinli\u011fi ve \u00f6nemli yan etkileri nedeniyle, Ligusticum barbarum yapraklar\u0131n\u0131n lipit d\u00fc\u015f\u00fcr\u00fcc\u00fc aktivitesi yava\u015f yava\u015f insanlar\u0131n dikkatini \u00e7ekmi\u015ftir. Yurti\u00e7i ve yurtd\u0131\u015f\u0131ndaki akademisyenler Ligusticum barbarum yapraklar\u0131n\u0131n kimyasal bile\u015fenleri \u00fczerine kapsaml\u0131 ara\u015ft\u0131rmalar y\u00fcr\u00fctm\u00fc\u015ft\u00fcr, ancak lipit d\u00fc\u015f\u00fcr\u00fcc\u00fc aktif bile\u015fenleri aramak i\u00e7in zaman al\u0131c\u0131, karma\u015f\u0131k i\u015flem ve aktif bile\u015fen kayb\u0131 gibi dezavantajlar\u0131 olan geleneksel kimyasal ay\u0131rma y\u00f6ntemleri kullan\u0131lm\u0131\u015ft\u0131r.<br \/>\n\u0130nce tabaka kromatografisi biyol\u00fcminesans, tan\u0131mlama, ay\u0131rma ve aktivite belirlemeyi entegre eden bir ila\u00e7 tarama tekni\u011fidir. Son 20 y\u0131lda, antioksidanlar\u0131n, kolinesteraz inhibit\u00f6rlerinin ve lipaz inhibit\u00f6rlerinin h\u0131zl\u0131 taranmas\u0131 i\u00e7in yayg\u0131n olarak kullan\u0131lm\u0131\u015ft\u0131r. Ayn\u0131 zamanda, bu y\u00f6ntem basit kullan\u0131m ve d\u00fc\u015f\u00fck deneysel maliyet \u00f6zelliklerine sahiptir. Do\u011frudan iyonizasyon teknolojisi, karma\u015f\u0131k matris \u00f6rneklerinin atmosferik bas\u0131n\u00e7ta \u00f6n i\u015fleme tabi tutulmadan h\u0131zl\u0131 k\u00fctle spektrometresi analizi i\u00e7in kullan\u0131lan yeni bir iyonizasyon tekni\u011fini ifade eder. Elektrostatik alan kaynakl\u0131 sprey iyonizasyon \u00e7ok a\u015famal\u0131 k\u00fctle spektrometrisi (TLC-EFISI-MSn) ile birle\u015ftirilmi\u015f ince tabaka kromatografisi, Lycium barbarum yapraklar\u0131n\u0131n ince tabaka otoradyografisinde aktif bile\u015fenlerin yerinde yap\u0131sal karakterizasyonunu ger\u00e7ekle\u015ftirebilir. Bu do\u011frudan iyonizasyon teknolojisi, ek gaz ve \u0131s\u0131tma cihazlar\u0131 olmadan mikro damlac\u0131k ekstraksiyonu yoluyla ince tabaka kromatografi plakalar\u0131ndaki bile\u015fikleri \u00e7\u0131karabilir ve analiz i\u00e7in k\u00fctle spektrometresine girmeden \u00f6nce bunlar\u0131 do\u011frudan bir elektrostatik alan\u0131n etkisi alt\u0131nda iyonize edebilir; s\u00f6k\u00fclmesi, \u00e7al\u0131\u015ft\u0131r\u0131lmas\u0131 ve h\u0131zl\u0131 bir \u015fekilde tespit edilmesi kolay bir iyonizasyon y\u00f6ntemidir. Bu nedenle, bu makale Ligustrum lucidum yapraklar\u0131ndan lipid d\u00fc\u015f\u00fcr\u00fcc\u00fc aktiviteye sahip bile\u015fenleri h\u0131zl\u0131 bir \u015fekilde tan\u0131mlamak i\u00e7in ince tabaka kromatografisi biyolojik otoradyografi k\u00fctle spektrometresi y\u00f6ntemini benimsemekte ve Ligustrum lucidum yapraklar\u0131n\u0131n lipid d\u00fc\u015f\u00fcr\u00fcc\u00fc etkisinin madde temelini a\u00e7\u0131kl\u0131\u011fa kavu\u015fturmak i\u00e7in bir temel sa\u011flamaktad\u0131r.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-7967\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-341.png\" alt=\"\" width=\"331\" height=\"210\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-7968\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/2-286.png\" alt=\"\" width=\"680\" height=\"275\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-7969\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/3-267.png\" alt=\"\" width=\"708\" height=\"773\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/3-267.png 708w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/3-267-659x720.png 659w\" sizes=\"(max-width: 708px) 100vw, 708px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-7970\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/4-247.png\" alt=\"\" width=\"708\" height=\"470\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/4-247.png 708w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/4-247-325x217.png 325w\" sizes=\"(max-width: 708px) 100vw, 708px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-7971\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/5-220.png\" alt=\"\" width=\"694\" height=\"497\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-7972\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/6-20.jpg\" alt=\"\" width=\"652\" height=\"330\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/6-20.jpg 652w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/6-20-650x330.jpg 650w\" sizes=\"(max-width: 652px) 100vw, 652px\" \/><br \/>\nBu makale ilk olarak Ligustrum lucidum yapraklar\u0131n\u0131n test \u00f6rne\u011fini iki sistemde geli\u015ftirmi\u015ftir: kloroform etil asetat metanol su (1:3:1:0.3, V\/V\/V\/V) ve etil asetat metanol su (6:2:1, V\/V\/V) daha fazla nokta elde etmek i\u00e7in. \u0130nce tabaka kromatografisi lipaz inhibisyonu biyol\u00fcminesans incelemesi yoluyla, Ligustrum lucidum yapraklar\u0131nda lipaz inhibisyon aktivitesine sahip 10 nokta taranm\u0131\u015f ve ard\u0131ndan yukar\u0131daki noktalar\u0131n h\u0131zl\u0131 yap\u0131sal analizi i\u00e7in TLC EFISI MSn teknolojisi kullan\u0131lm\u0131\u015ft\u0131r. Literat\u00fcr raporlar\u0131yla birle\u015ftirilen \u00e7ok a\u015famal\u0131 k\u00fctle spektrometresi verilerine dayanarak, farkl\u0131 bile\u015fiklerin olas\u0131 par\u00e7alanma yollar\u0131 tahmin edildi ve 9 arbutin triterpenoid, 1 oleanane triterpenoid ve 5 18,19 halkal\u0131 triterpenoid dahil olmak \u00fczere hepsi triterpenoid saponin olan 15 olas\u0131 yap\u0131 \u00f6n karakterize edildi. Referans madde ile kar\u015f\u0131la\u015ft\u0131r\u0131ld\u0131ktan sonra 12 bile\u015fik do\u011frulanm\u0131\u015ft\u0131r. Bu y\u00f6ntem, Cornus officinalis yapraklar\u0131ndan lipaz inhibit\u00f6r aktivitesi olan bile\u015fenleri h\u0131zl\u0131 bir \u015fekilde tarayabilir ve karakterize edebilir, Cornus officinalis yapraklar\u0131n\u0131n lipit d\u00fc\u015f\u00fcr\u00fcc\u00fc etkinli\u011finin madde temelini a\u00e7\u0131kl\u0131\u011fa kavu\u015fturmak i\u00e7in bir temel sa\u011flar.<\/p>","protected":false},"excerpt":{"rendered":"<p>Characterization of Lipase Inhibiting Active Components in Gougu Leaves by Thin Layer Chromatography Bioautography MSn Method Ilex cornuta Lindl Ex Paxt is a plant of the genus Ilex in the family Ilex, widely distributed in China. The dried leaves of Gougu, a traditional Chinese medicine, have been included in many herbal monographs since the Tang [&hellip;]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.0 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Characterization of Lipase Inhibiting Active Components in Gougu Leaves by Thin Layer Chromatography Bioautography MSn Method - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/tr\/gougu-yapraklari\/\" \/>\n<meta property=\"og:locale\" content=\"tr_TR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Characterization of Lipase Inhibiting Active Components in Gougu Leaves by Thin Layer Chromatography Bioautography MSn Method - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"Characterization of Lipase Inhibiting Active Components in Gougu Leaves by Thin Layer Chromatography Bioautography MSn Method Ilex cornuta Lindl Ex Paxt is a plant of the genus Ilex in the family Ilex, widely distributed in China. 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