{"id":7760,"date":"2024-08-08T11:06:18","date_gmt":"2024-08-08T11:06:18","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=7760"},"modified":"2024-08-08T11:06:18","modified_gmt":"2024-08-08T11:06:18","slug":"amylase","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/tr\/amilaz\/","title":{"rendered":"Alfa-amilaz, beta-amilaz ve glikozile amilaz aras\u0131ndaki fark nedir?"},"content":{"rendered":"

\u03b1-amilaz, \u03b2-amilaz ve sakkarifikasyon amilaz diyagram\u0131n\u0131n rol\u00fcne g\u00f6re, insanlar\u0131n yanl\u0131\u015fl\u0131kla \u00fc\u00e7 substrat\u0131n rol\u00fcn\u00fcn s\u0131ras\u0131yla ni\u015fasta, dekstrin ve maltoz olmas\u0131 gerekti\u011fini d\u00fc\u015f\u00fcnmelerine izin vermek kolayd\u0131r, bir zamanlar bana sakkarifikasyon amilaz\u0131n maltoz enzimi olmad\u0131\u011f\u0131n\u0131 soran \u00e7ocuklar var. Bu nedenle, \u00fc\u00e7\u00fcn\u00fcn rol\u00fc hakk\u0131nda k\u00fc\u00e7\u00fck bir analiz yapman\u0131n gerekli oldu\u011funu d\u00fc\u015f\u00fcn\u00fcyorum.<\/p>\n

\"\"<\/p>\n

\u00c7e\u015fitli amilaz t\u00fcrlerinin rol\u00fcn\u00fcn anla\u015f\u0131lmas\u0131n\u0131 kolayla\u015ft\u0131rmak i\u00e7in \u00f6ncelikle iki kavram setinin tan\u0131mlanmas\u0131 gerekmektedir: d\u00fcz zincire kar\u015f\u0131 dallanm\u0131\u015f ni\u015fasta ve \u03b1-1,4-glikozidik ve \u03b1-1,6-glikozidik ba\u011flar.<\/strong><\/p>\n

\u0130ki t\u00fcr ni\u015fasta vard\u0131r: d\u00fcz zincirli ni\u015fasta ve dall\u0131 zincirli ni\u015fasta. D\u00fcz zincirli amilopektin, sadece ba\u015ftan kuyru\u011fa \u03b1-1,4-glikozidik ba\u011flarla ba\u011flanan glikoz kal\u0131nt\u0131lar\u0131ndan olu\u015fan dallanmam\u0131\u015f sarmal bir yap\u0131d\u0131r; dall\u0131 zincirli amilopektin, her zincirde ba\u015ftan kuyru\u011fa \u03b1-1,4-glikozidik ba\u011flarla ba\u011flanan glikoz kal\u0131nt\u0131lar\u0131ndan olu\u015fur, ancak dallarda \u03b1-1,6-glikozidik ba\u011flar bulunur.<\/p>\n

\u03b1-Amilaz ve rol\u00fc<\/strong><\/p>\n

Endo amilaz olarak da bilinen \u03b1- Amilaz, aktivite g\u00f6stermek i\u00e7in enzim proteinine ba\u011flanan kalsiyum iyonlar\u0131na ihtiya\u00e7 duyan bir metalohidrolazd\u0131r ve bu iyonlar \u015felatlama ajan\u0131 EDTA ile muamele edilerek kaybedilebilir.<\/p>\n

\u03b1- Amilaz, ni\u015fasta i\u00e7indeki \u03b1-1,4-glikozidik ba\u011f\u0131 rastgele hidrolize edebilir, ancak \u03b1-1,6-glikozidik ba\u011f\u0131 hidrolize edemez.<\/strong><\/p>\n

Substrat d\u00fcz zincirli ni\u015fasta ise, hidroliz glikoz, maltoz ve maltotrioz \u00fcretir; etkili substrat dall\u0131 zincirli ni\u015fasta ise, hidroliz \u00fcr\u00fcnleri glikoz, maltoz, maltotrioz ve \u00fc\u00e7ten fazla glikoz kal\u0131nt\u0131s\u0131 i\u00e7eren \u03b1-1,6-glikozidik ba\u011flar i\u00e7eren \u03b1-dekstrindir.<\/strong><\/p>\n

Ayr\u0131ca, \u03b1-amilaz ve \u03b2-amilazdaki \u03b1 ve \u03b2'n\u0131n herhangi bir konformasyonel ili\u015fkiyi g\u00f6stermedi\u011fi, sadece numaraland\u0131r\u0131ld\u0131\u011f\u0131 unutulmamal\u0131d\u0131r.<\/p>\n

Do\u011fada \u03b1-amilaz \u00fcretmenin \u00e7e\u015fitli yollar\u0131 vard\u0131r ve \u00f6rne\u011fin mikrobiyal fermantasyon en yayg\u0131n yollardan biridir. Ayr\u0131ca bu enzim bitkilerden veya hayvanlardan da elde edilebilir ve farkl\u0131 yollarla elde edilen amilaz\u0131n \u00f6zellikleri farkl\u0131d\u0131r. End\u00fcstriyel \u00fcretim s\u00fcrecinde, \u03b1-amilaza olan b\u00fcy\u00fck talep nedeniyle, genellikle mantar ve bakterilerin fermantasyonu ile yap\u0131l\u0131r. Bacillus subtilis, Bacillus sphaericus ve Streptomyces sphaericus \u03b1-amilaz \u00fcretebilir.<\/p>\n

\u03b2-Amilaz ve i\u015flevi<\/strong><\/p>\n

Ekzoamilaz olarak da bilinen \u03b2-amilaz, s\u00fclfhidril gruplar\u0131 i\u00e7eren bir hidrolazd\u0131r. Enzim ni\u015fastan\u0131n indirgeyici olmayan ucundan ba\u015flar ve \u03b1-1,4-glikozidik ba\u011f\u0131 s\u0131rayla iki glikoz kal\u0131nt\u0131s\u0131 ile hidrolize ederek maltoz \u00fcretir. \u03b1-1,6-glikozidik ba\u011f\u0131 hidrolize edemez ve ayr\u0131ca dallanma noktas\u0131n\u0131 ge\u00e7emez ve \u00e7ok uzun bir dekstrin, yani \u03b2-dekstrin b\u0131rak\u0131r.<\/p>\n

Bu nedenle, \u03b2-amilaz d\u00fcz zincirli ni\u015fastalar \u00fczerinde etkili oldu\u011funda, neredeyse sadece maltoz \u00fcretir; dall\u0131 zincirli ni\u015fastalar \u00fczerinde kullan\u0131ld\u0131\u011f\u0131nda, \u00fcr\u00fcnler maltoz ve \u03b2-dekstrindir.<\/strong><\/p>\n

R-enzimi ve etkisi<\/strong><\/p>\n

Ay\u0131r\u0131c\u0131 enzimler olarak da adland\u0131r\u0131lan R-enzimleri, \u03b1-1,6-glikozidik ba\u011f \u00fczerinde etkilidir. Ay\u0131r\u0131c\u0131 enzimlerin varl\u0131\u011f\u0131nda, \u03b1-dekstrin ve \u03b2-dekstrin t\u00fcrlerinin \u03b1-1,6-glikozidik ba\u011f\u0131 hidrolize olur ve dallanan dall\u0131 zincirler \u00e7\u0131kar\u0131l\u0131r.<\/p>\n

Kalan d\u00fcz zincirler daha sonra \u03b1-amilaz ve \u03b2-amilaz taraf\u0131ndan hidrolize edilerek maltoz ve glukoz \u00fcretilir. Ancak, R-amilazlar dallanm\u0131\u015f ni\u015fastalar i\u00e7indeki \u03b1-1,6-glikozidik ba\u011flar\u0131 do\u011frudan hidrolize edemez.<\/p>\n

Glikozile edici amilaz ve etkisi<\/strong><\/p>\n

Glikozile edici amilaz veya glukoamilaz, ni\u015fastay\u0131 glikoza hidrolize eden bir enzimdir, burada hidroliz y\u00fczdesi 100%'ye ula\u015fabilir ve genellikle ni\u015fasta i\u00e7in bir sakarize edici ajan olarak kullan\u0131l\u0131r. Glukoamilaz \u00e7ok spesifik de\u011fildir ve bir\u00e7ok maddeye uygulanabilir, sadece ni\u015fasta molek\u00fcl\u00fcn\u00fcn indirgeyici olmayan ucundan \u03b1-1,4- glikozidik ba\u011f\u0131 kesmekle kalmaz, ayn\u0131 zamanda \u03b1-1,6-glikozidik ba\u011f\u0131 da kesebilir, sadece \u03b1-1,4-glikozidik ba\u011f\u0131n hidrolizi biraz daha h\u0131zl\u0131d\u0131r. Ayr\u0131ca dekstrin, maltoz ve glikojeni hidrolize eder<\/strong>. Hidroliz, substrat molek\u00fcl\u00fcn\u00fcn ucundan ba\u015flat\u0131l\u0131r ve bir ekzon\u00fckleazd\u0131r.<\/p>\n

\u03b1-amilaz, \u03b2-amilaz ve glikozile amilaz\u0131n dall\u0131 zincirli ni\u015fasta \u00fczerindeki etkisinin sonu\u00e7lar\u0131 a\u015fa\u011f\u0131daki diyagramda g\u00f6sterilebilir:<\/p>\n

\"\"<\/p>","protected":false},"excerpt":{"rendered":"

According to the role of \u03b1-amylase, \u03b2-amylase and saccharification amylase diagram is easy to let people mistakenly think that the role of the three substrates must be starch, dextrin and maltose, respectively, there are children once asked me saccharification amylase is not maltose enzyme. Therefore, I think it is necessary to do a little analysis […]<\/p>","protected":false},"author":4,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[46],"tags":[],"yoast_head":"\nWhat is the difference between alpha-amylase, beta-amylase, and glycosylated amylase? - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/tr\/amilaz\/\" \/>\n<meta property=\"og:locale\" content=\"tr_TR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"What is the difference between alpha-amylase, beta-amylase, and glycosylated amylase? - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"According to the role of \u03b1-amylase, \u03b2-amylase and saccharification amylase diagram is easy to let people mistakenly think that the role of the three substrates must be starch, dextrin and maltose, respectively, there are children once asked me saccharification amylase is not maltose enzyme. Therefore, I think it is necessary to do a little analysis […]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/longchangextracts.com\/tr\/amilaz\/\" \/>\n<meta property=\"og:site_name\" content=\"China Chemical Manufacturer\" \/>\n<meta property=\"article:published_time\" content=\"2024-08-08T11:06:18+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-331.png\" \/>\n<meta name=\"author\" content=\"Mrzhao\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Yazan:\" \/>\n\t<meta name=\"twitter:data1\" content=\"Mrzhao\" \/>\n\t<meta name=\"twitter:label2\" content=\"Tahmini okuma s\u00fcresi\" \/>\n\t<meta name=\"twitter:data2\" content=\"4 dakika\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/longchangextracts.com\/amylase\/\",\"url\":\"https:\/\/longchangextracts.com\/amylase\/\",\"name\":\"What is the difference between alpha-amylase, beta-amylase, and glycosylated amylase? - China Chemical Manufacturer\",\"isPartOf\":{\"@id\":\"https:\/\/longchangextracts.com\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/longchangextracts.com\/amylase\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/longchangextracts.com\/amylase\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-331.png\",\"datePublished\":\"2024-08-08T11:06:18+00:00\",\"dateModified\":\"2024-08-08T11:06:18+00:00\",\"author\":{\"@id\":\"https:\/\/longchangextracts.com\/#\/schema\/person\/80f128fa4967f2080f7577af46a2bfb3\"},\"breadcrumb\":{\"@id\":\"https:\/\/longchangextracts.com\/amylase\/#breadcrumb\"},\"inLanguage\":\"tr\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/longchangextracts.com\/amylase\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"tr\",\"@id\":\"https:\/\/longchangextracts.com\/amylase\/#primaryimage\",\"url\":\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-331.png\",\"contentUrl\":\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-331.png\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/longchangextracts.com\/amylase\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/longchangextracts.com\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"What is the difference between alpha-amylase, beta-amylase, and glycosylated amylase?\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/longchangextracts.com\/#website\",\"url\":\"https:\/\/longchangextracts.com\/\",\"name\":\"China Chemical Manufacturer\",\"description\":\"\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/longchangextracts.com\/?s={search_term_string}\"},\"query-input\":\"required name=search_term_string\"}],\"inLanguage\":\"tr\"},{\"@type\":\"Person\",\"@id\":\"https:\/\/longchangextracts.com\/#\/schema\/person\/80f128fa4967f2080f7577af46a2bfb3\",\"name\":\"Mrzhao\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"tr\",\"@id\":\"https:\/\/longchangextracts.com\/#\/schema\/person\/image\/\",\"url\":\"https:\/\/secure.gravatar.com\/avatar\/5b7d172a0978697481379e6a4a769a17?s=96&d=mm&r=g\",\"contentUrl\":\"https:\/\/secure.gravatar.com\/avatar\/5b7d172a0978697481379e6a4a769a17?s=96&d=mm&r=g\",\"caption\":\"Mrzhao\"}}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Alfa-amilaz, beta-amilaz ve glikozile amilaz aras\u0131ndaki fark nedir? - \u00c7in Kimyasal \u00dcreticisi","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/longchangextracts.com\/tr\/amilaz\/","og_locale":"tr_TR","og_type":"article","og_title":"What is the difference between alpha-amylase, beta-amylase, and glycosylated amylase? - China Chemical Manufacturer","og_description":"According to the role of \u03b1-amylase, \u03b2-amylase and saccharification amylase diagram is easy to let people mistakenly think that the role of the three substrates must be starch, dextrin and maltose, respectively, there are children once asked me saccharification amylase is not maltose enzyme. 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