{"id":7351,"date":"2024-08-06T19:33:03","date_gmt":"2024-08-06T19:33:03","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=7351"},"modified":"2024-08-06T19:33:03","modified_gmt":"2024-08-06T19:33:03","slug":"verbena-officinalis","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/tr\/verbena-officinalis\/","title":{"rendered":"Verbena officinalis \u015fifal\u0131 bitkisi i\u00e7in UPLC parmak izi spektrumunun olu\u015fturulmas\u0131 ve indikat\u00f6r bile\u015fenlerin belirlenmesi"},"content":{"rendered":"<p>Verbena officinalis \u015fifal\u0131 bitkisi i\u00e7in UPLC parmak izi spektrumunun olu\u015fturulmas\u0131 ve indikat\u00f6r bile\u015fenlerin belirlenmesi<br \/>\nVerbenaceae familyas\u0131ndan bir bitki olan Verbena officinalis L. bitkisinin kurutulmu\u015f toprak \u00fcst\u00fc k\u0131sm\u0131d\u0131r. Kan dola\u015f\u0131m\u0131n\u0131 te\u015fvik etme, kan staz\u0131n\u0131 da\u011f\u0131tma, detoksifikasyon, di\u00fcrez, sararmay\u0131 azaltma ve s\u0131tmay\u0131 \u00f6nleme etkilerine sahiptir. Kimyasal bile\u015fim \u00e7al\u0131\u015fmalar\u0131, mine \u00e7i\u00e7e\u011finin u\u00e7ucu ya\u011flar, iridoidler, fenilpropanoidler, flavonoidler ve anti-enflamatuar, antioksidan, analjezik, \u00f6ks\u00fcr\u00fck ve ast\u0131m giderici, anti miyokardiyal iskemi ve serebral iskemi gibi \u00e7e\u015fitli farmakolojik aktivitelere sahip di\u011fer bile\u015fenleri i\u00e7erdi\u011fini g\u00f6stermi\u015ftir. \u00c7in Halk Cumhuriyeti Farmakopesi'nin 2020 bask\u0131s\u0131, Birinci B\u00f6l\u00fcm, mine \u00e7i\u00e7e\u011finin t\u0131bbi bitkisi i\u00e7in i\u00e7erik g\u00f6stergeleri olarak oleanolik asit ve ursolik asit kullan\u0131r, ancak spesifikli\u011fi g\u00fc\u00e7l\u00fc de\u011fildir. Dahas\u0131, geleneksel \u00c7in t\u0131bb\u0131nda geleneksel dekoksiyonlar kullan\u0131lmakta ve oleanolik asit ve ursolik asit gibi lipofilik bile\u015fenler i\u00e7erik g\u00f6stergeleri olarak kullan\u0131lmaktad\u0131r. Bunun \u015fifal\u0131 bitkilerin kalite kontrol\u00fcne yard\u0131mc\u0131 olup olmad\u0131\u011f\u0131 tart\u0131\u015fmaya de\u011ferdir. Verbascoside ve verbascoside gibi sikloheksen eter terpenoid glikozitlerin yan\u0131 s\u0131ra verbascoside'deki fenilpropanoid glikozit, verbascoside'in y\u00fcksek i\u00e7eri\u011fini \u00f6l\u00e7erek, verbascoside'in klinik etkinli\u011fini sa\u011flamak i\u00e7in belirli bir \u00f6zg\u00fcll\u00fc\u011fe ve pozitif \u00f6neme sahip oldu\u011funa dair literat\u00fcr raporlar\u0131 vard\u0131r. Yukar\u0131daki i\u00e7erik g\u00f6stergeleri verbascoside standart kaynatma ve form\u00fcl gran\u00fcllerinin kalite kontrol ara\u015ft\u0131rmalar\u0131nda kullan\u0131lm\u0131\u015ft\u0131r. Parmak izi spektrumlar\u0131 da verbascoside'in kalite kontrol\u00fc i\u00e7in kullan\u0131lm\u0131\u015ft\u0131r, ancak kimyasal bile\u015fenlerin tan\u0131mlanmas\u0131 esas olarak verbascoside, verbascoside ve verbascoside \u00fczerine odaklanm\u0131\u015ft\u0131r. Suda \u00e7\u00f6z\u00fcnen di\u011fer bile\u015fenlerin \u00e7al\u0131\u015f\u0131lmas\u0131na ili\u015fkin herhangi bir rapor bulunmamaktad\u0131r. Bu \u00e7al\u0131\u015fmada, mine \u00e7i\u00e7e\u011fi bitkisinin parmak izi spektrumunun olu\u015fturulmas\u0131 ve y\u00fcksek \u00e7\u00f6z\u00fcn\u00fcrl\u00fckl\u00fc k\u00fctle spektrometresi tan\u0131mlamas\u0131n\u0131n yan\u0131 s\u0131ra d\u00f6rt aktif bile\u015fen, yani verbascoside, verbascoside, verbascoside ve isoverbascoside i\u00e7in i\u00e7erik belirleme y\u00f6ntemlerinin olu\u015fturulmas\u0131, mine \u00e7i\u00e7e\u011fi bitkisinin genel kalite kontrol\u00fc i\u00e7in referans sa\u011flamak amac\u0131yla kullan\u0131lm\u0131\u015ft\u0131r.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-7352\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-297.png\" alt=\"\" width=\"356\" height=\"387\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-7353\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/2-31.jpg\" alt=\"\" width=\"643\" height=\"292\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-7354\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/3-24.jpg\" alt=\"\" width=\"664\" height=\"251\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-7355\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/4-214.png\" alt=\"\" width=\"365\" height=\"370\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-7356\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/5-193.png\" alt=\"\" width=\"350\" height=\"526\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-7357\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/6-16.jpg\" alt=\"\" width=\"651\" height=\"353\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-7358\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/7-10.jpg\" alt=\"\" width=\"657\" height=\"283\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-7359\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/8-8.jpg\" alt=\"\" width=\"639\" height=\"236\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-7360\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/9-6.jpg\" alt=\"\" width=\"653\" height=\"235\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/9-6.jpg 653w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/9-6-650x235.jpg 650w\" sizes=\"(max-width: 653px) 100vw, 653px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-7361\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/10-10.jpg\" alt=\"\" width=\"645\" height=\"241\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-7362\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/11-6.jpg\" alt=\"\" width=\"638\" height=\"239\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-7363\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/12-6.jpg\" alt=\"\" width=\"642\" height=\"239\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-7364\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/13-4.jpg\" alt=\"\" width=\"657\" height=\"175\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-7365\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/14-2.jpg\" alt=\"\" width=\"666\" height=\"380\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-7366\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/15-4.jpg\" alt=\"\" width=\"657\" height=\"470\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-7367\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/16-2.jpg\" alt=\"\" width=\"663\" height=\"501\" \/><br \/>\n\u0130\u00e7erik g\u00f6stergelerinin se\u00e7imi. Literat\u00fcr raporlar\u0131na ve \u00f6nceki ara\u015ft\u0131rmalar\u0131m\u0131za g\u00f6re, verbascoside belirli ko\u015fullar alt\u0131nda isoverbascoside'e d\u00f6n\u00fc\u015ft\u00fcr\u00fclebilir. Hem verbascoside hem de isoverbascoside antioksidan, anti hemolitik, anti anjiyotensinaz aktivitesi, kan bas\u0131nc\u0131n\u0131 d\u00fc\u015f\u00fcr\u00fcc\u00fc ve ba\u011f\u0131\u015f\u0131kl\u0131k fonksiyonunu artt\u0131r\u0131c\u0131 etkilere sahiptir. Bu da verbascoside ve isoverbascoside'in mine \u00e7i\u00e7e\u011finin kalite kontrol\u00fc ve de\u011ferlendirilmesi i\u00e7in b\u00fcy\u00fck \u00f6nem ta\u015f\u0131d\u0131\u011f\u0131n\u0131 g\u00f6stermektedir. Bu nedenle, mevcut ara\u015ft\u0131rmalara dayanarak, \u015firketimiz mine \u00e7i\u00e7e\u011fi i\u00e7eren \u00c7in bitkisel preparatlar\u0131n\u0131n kalite kontrol\u00fc i\u00e7in referans sa\u011flamak amac\u0131yla izoverbascoside tayinini art\u0131racakt\u0131r. Kimyasal bile\u015fim \u00e7al\u0131\u015fmalar\u0131, hem verbascoside hem de verbascoside'in iridoid glikozitler oldu\u011funu g\u00f6stermi\u015ftir. 5-hidroksi verbascoside olarak da bilinen verbascoside, verbascoside ile kar\u015f\u0131la\u015ft\u0131r\u0131ld\u0131\u011f\u0131nda karbon pozisyonu 5'te ek bir hidroksil grubuna sahiptir. \u0130ki bile\u015fenin kimyasal yap\u0131lar\u0131 benzerdir ve UV g\u00f6r\u00fcn\u00fcr 3D spektrumlar\u0131ndaki absorpsiyon e\u011frileri temelde ayn\u0131d\u0131r. Bu nedenle, tespit i\u00e7in iki bile\u015fenin birle\u015ftirilmesi ve iki bile\u015fenin toplam miktar\u0131n\u0131n hesaplanmas\u0131 \u00f6nerilir. Bu \u00e7al\u0131\u015fmada, aktif bile\u015fenlerin i\u00e7eri\u011fini belirlemek i\u00e7in d\u00f6rt y\u00f6ntem olu\u015fturulmu\u015ftur. Test \u00e7\u00f6zeltisinin haz\u0131rlanma y\u00f6ntemi parmak izi spektrumu ile tutarl\u0131yd\u0131, ancak kromatografik ko\u015fullar ayr\u0131 olarak olu\u015fturuldu ve parmak izi spektrumu ile tutarl\u0131 de\u011fildi. Ana hususlar, indikat\u00f6r bile\u015fenlerinin ayr\u0131lma derecesi, safl\u0131\u011f\u0131 ve y\u00f6ntemin tekrar \u00fcretilebilirli\u011fiydi. \u0130\u00e7erik belirleme sonu\u00e7lar\u0131, mine \u00e7i\u00e7e\u011fi bitkisinin farkl\u0131 \u00fcretim alanlar\u0131nda verbascoside, verbascoside, verbascoside ve isoverbascoside i\u00e7eri\u011finde belirli farkl\u0131l\u0131klar oldu\u011funu g\u00f6stermi\u015ftir. Ayn\u0131 \u00fcretim alan\u0131 i\u00e7inde bile, farkl\u0131 bitki partilerinin i\u00e7eri\u011finde farkl\u0131l\u0131klar vard\u0131 ve bu farkl\u0131l\u0131klar temel olarak bitkilerin hasat zaman\u0131, i\u015fleme y\u00f6ntemleri, depolama y\u00f6ntemleri ve depolama s\u00fcresindeki farkl\u0131l\u0131klarla ilgiliydi. Buna ek olarak, \u015firketimiz ara\u015ft\u0131rmam\u0131zda ayn\u0131 mine \u00e7i\u00e7e\u011fi bitkisinin farkl\u0131 t\u0131bbi k\u0131s\u0131mlar\u0131nda verbascoside, verbascoside, verbascoside ve isoverbascoside i\u00e7eri\u011finde \u00f6nemli farkl\u0131l\u0131klar oldu\u011funu tespit etmi\u015ftir. Bunlar aras\u0131nda, \u00e7i\u00e7ek ve yapraklardaki verbascoside, verbascoside, verbascoside ve isoverbascoside i\u00e7eri\u011fi, g\u00f6vdelerdekinden \u00f6nemli \u00f6l\u00e7\u00fcde daha y\u00fcksektir. Bu nedenle, \u015fifal\u0131 bitkilerin hasat zaman\u0131n\u0131n s\u0131k\u0131 bir \u015fekilde kontrol edilmesi ve bitkilerdeki \u00e7i\u00e7ek ve yaprak oran\u0131n\u0131n sa\u011flanmas\u0131, mine \u00e7i\u00e7e\u011fi \u015fifal\u0131 bitkilerinin kalite kontrol\u00fc i\u00e7in b\u00fcy\u00fck \u00f6nem ta\u015f\u0131maktad\u0131r.<br \/>\nParmak izi spektrumlar\u0131 \u00fczerine ara\u015ft\u0131rma. Herba Verbena'n\u0131n parmak izi spektrumunu olu\u015fturmak i\u00e7in UPLC y\u00f6ntemi kullan\u0131lm\u0131\u015ft\u0131r. Parmak izi spektrumunun kromatografik ko\u015fullar\u0131 ve test \u00e7\u00f6zeltisinin haz\u0131rlanma y\u00f6ntemi sistematik olarak ara\u015ft\u0131r\u0131lm\u0131\u015ft\u0131r. Benzerlik hesaplama sonu\u00e7lar\u0131, 15 parti t\u0131bbi malzemenin parmak izi spektrumlar\u0131n\u0131n benzerli\u011finin 0,9'un \u00fczerinde oldu\u011funu ve genel benzerli\u011fin y\u00fcksek oldu\u011funu ve bunun Herba Verbena'n\u0131n tan\u0131mlanmas\u0131 ve kalite kontrol\u00fc i\u00e7in kullan\u0131labilece\u011fini g\u00f6stermi\u015ftir. Ortak pikleri tan\u0131mlamak i\u00e7in y\u00fcksek \u00e7\u00f6z\u00fcn\u00fcrl\u00fckl\u00fc k\u00fctle spektrometresi kullan\u0131larak, iridoid glikozitler, fenilpropanoid glikozitler ve flavonoidler olmak \u00fczere \u00fc\u00e7 t\u00fcr aktif bile\u015fen tan\u0131mlanm\u0131\u015ft\u0131r. \u0130lk kez, mine \u00e7i\u00e7e\u011findeki \u00fc\u00e7 t\u00fcr aktif bile\u015feni karakterize etmek i\u00e7in parmak izi spektrumlar\u0131 kullan\u0131ld\u0131 ve ilk kez, mine \u00e7i\u00e7e\u011finin parmak izi spektrumundan, mine \u00e7i\u00e7e\u011finin genel b\u00fct\u00fcnl\u00fc\u011f\u00fcn\u00fc ve \u00f6zelliklerini daha iyi yans\u0131tan isoverbascoside, luteolin 7-diglucoside ve apigenin 7-O-glucuronide tan\u0131mland\u0131. Ortak piklerin g\u00f6receli pik alan\u0131n\u0131 de\u011fi\u015fken olarak kullanan k\u00fcme analizi ve temel bile\u015fen analizi, 15 parti t\u0131bbi malzemeyi \u00fc\u00e7 kategoride s\u0131n\u0131fland\u0131rm\u0131\u015ft\u0131r. Bunlar aras\u0131nda Henan, Jiangsu ve Guizhou'dan gelen t\u0131bbi malzemeler benzer kimyasal bile\u015fim oranlar\u0131na sahipti. OPLS-DA, pik oranlar\u0131nda \u00f6nemli farkl\u0131l\u0131klar g\u00f6steren yedi kromatografik pik bulmu\u015ftur; bunlar pik 2, 6, 16, 10, 11, 5 ve 9'dur. Bu farkl\u0131l\u0131klar, t\u0131bbi malzemelerin hasat zaman\u0131 ve i\u015fleme y\u00f6ntemlerindeki farkl\u0131l\u0131klarla ilgili olabilir.<br \/>\n\u00d6zetle, bu y\u00f6ntem farkl\u0131 k\u00f6kenlerden gelen \u00c7in \u015fifal\u0131 bitkilerinin kalite farkl\u0131l\u0131klar\u0131n\u0131 etkili bir \u015fekilde analiz edebilir ve farkl\u0131 k\u00f6kenlerden gelen \u00c7in \u015fifal\u0131 bitkilerinin kalitesinin de\u011ferlendirilmesi i\u00e7in bir referans sa\u011flayabilir.<\/p>","protected":false},"excerpt":{"rendered":"<p>Establishment of UPLC fingerprint spectrum and determination of indicator components for the medicinal herb of Verbena officinalis Verbena officinalis L., a plant in the Verbenaceae family, is the dried aboveground part of the plant. It has the effects of promoting blood circulation, dispersing blood stasis, detoxification, diuresis, reducing yellowing, and intercepting malaria. Chemical composition studies [&hellip;]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.0 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Establishment of UPLC fingerprint spectrum and determination of indicator components for the medicinal herb of Verbena officinalis - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/tr\/verbena-officinalis\/\" \/>\n<meta property=\"og:locale\" content=\"tr_TR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Establishment of UPLC fingerprint spectrum and determination of indicator components for the medicinal herb of Verbena officinalis - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"Establishment of UPLC fingerprint spectrum and determination of indicator components for the medicinal herb of Verbena officinalis Verbena officinalis L., a plant in the Verbenaceae family, is the dried aboveground part of the plant. It has the effects of promoting blood circulation, dispersing blood stasis, detoxification, diuresis, reducing yellowing, and intercepting malaria. 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