{"id":6879,"date":"2024-08-06T14:59:46","date_gmt":"2024-08-06T14:59:46","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=6879"},"modified":"2024-08-06T14:59:46","modified_gmt":"2024-08-06T14:59:46","slug":"turnip-neutral-polysaccharides","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/tr\/salgam-notral-polisakkaritleri\/","title":{"rendered":"\u015ealgam n\u00f6tral polisakkaritlerinin PC12 h\u00fccrelerinde oksidatif hasara kar\u015f\u0131 koruyucu mekanizmas\u0131 \u00fczerine \u00f6n \u00e7al\u0131\u015fma"},"content":{"rendered":"

\u015ealgam n\u00f6tral polisakkaritlerinin PC12 h\u00fccrelerinde oksidatif hasara kar\u015f\u0131 koruyucu mekanizmas\u0131 \u00fczerine \u00f6n \u00e7al\u0131\u015fma
\nChagu veya Manjing olarak da bilinen \u015falgam (Brassica rapa L.), Brassicaceae familyas\u0131na ve Brassica cinsine aittir. K\u00f6kleri ile \u015fifal\u0131 bir bitki olarak kullan\u0131l\u0131r ve besin a\u00e7\u0131s\u0131ndan zengindir. Uzun s\u00fcre t\u00fcketilebilir ve \"uzun \u00f6m\u00fcr meyvesi\" olarak bilinir. \u0130\u015ftah a\u00e7\u0131c\u0131 ve sindirimi kolayla\u015ft\u0131r\u0131c\u0131, qi dola\u015f\u0131m\u0131n\u0131 te\u015fvik edici, \u00f6ks\u00fcr\u00fck ve ast\u0131m\u0131 giderici, di\u00fcrez ve detoksifikasyonu te\u015fvik edici etkileri vard\u0131r. Modern farmakolojik ara\u015ft\u0131rmalar, \u015falgam\u0131n kan \u015fekerini d\u00fc\u015f\u00fcrme, antioksidasyon, ya\u015flanma kar\u015f\u0131t\u0131, anti-t\u00fcm\u00f6r, ba\u011f\u0131\u015f\u0131kl\u0131\u011f\u0131 d\u00fczenleme ve anti-inflamatuar gibi farmakolojik aktivitelere sahip oldu\u011funu bulmu\u015ftur. \u00d6n ara\u015ft\u0131rmada, ara\u015ft\u0131rma ekibi \u015falgam polisakkaritlerinin i\u00e7eri\u011finin yakla\u015f\u0131k 11.53% oldu\u011funu \u00f6l\u00e7m\u00fc\u015ft\u00fcr. Ya\u011fdan ar\u0131nd\u0131rma ve renk giderme i\u015flemlerinden sonra suda \u00e7\u00f6z\u00fcnebilen \u015falgam polisakkaridi BRP elde edildi. Brassica rapa L.'den n\u00f6tral polisakkaritler (BRNP) ve Brassica rapa L.'den asidik polisakkaritler (BRAP) elde etmek i\u00e7in daha fazla izole edildi ve safla\u015ft\u0131r\u0131ld\u0131 ve yap\u0131lar\u0131 tan\u0131mland\u0131. \u00d6n deneyler, \u015falgam polisakkaritlerinin iyi antioksidan aktiviteye sahip oldu\u011funu g\u00f6stermi\u015ftir; bu da \u015falgam polisakkaritlerinin antioksidan ve ya\u015flanma kar\u015f\u0131t\u0131 ara\u015ft\u0131rmalarda uygulanmas\u0131 i\u00e7in deneysel bir temel olu\u015fturmaktad\u0131r. Bu nedenle, bu \u00e7al\u0131\u015fma, BRNP taraf\u0131ndan H2O2 ile ind\u00fcklenen hasardan sonra PC12 h\u00fccrelerinde Bax, Bcl-2 ve Caspase-3 proteinlerinin ekspresyon seviyelerini ara\u015ft\u0131rmak ve BRNP'nin ya\u015flanma kar\u015f\u0131t\u0131 mekanizmas\u0131n\u0131 daha da ayd\u0131nlatmak i\u00e7in H2O2 ile ind\u00fcklenen PC12 h\u00fccrelerinin erken ya\u015flanma modelini olu\u015fturmu\u015ftur.<\/p>\n

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\nH2O2, h\u00fccre i\u00e7i reaktif oksijen t\u00fcrleri i\u00e7eri\u011fini art\u0131rabilen ve h\u00fccrelerde hasara neden olabilen reaktif bir oksijen t\u00fcr\u00fc molek\u00fcl\u00fcd\u00fcr. K\u0131sa s\u00fcrede h\u00fccre ya\u015flanma modelleri elde etmek i\u00e7in yayg\u0131n olarak kullan\u0131lmaktad\u0131r. H\u00fccre proliferasyon aktivitesi, k\u00fclt\u00fcrlenmi\u015f h\u00fccrelerin belirli ko\u015fullar alt\u0131nda normal olarak \u00e7o\u011fal\u0131p \u00e7o\u011falamayaca\u011f\u0131n\u0131 belirlemek i\u00e7in \u00f6nemli bir g\u00f6stergedir. CCK-8 deney sonu\u00e7lar\u0131, PC12 h\u00fccrelerinin hayatta kalma oran\u0131n\u0131n H2O2 konsantrasyonunun artmas\u0131yla \u00f6nemli \u00f6l\u00e7\u00fcde azald\u0131\u011f\u0131n\u0131 ve doza ba\u011fl\u0131 bir etki sergiledi\u011fini g\u00f6stermi\u015ftir. 4 saat boyunca 300 \u03bc mol\/L H2O2 ile muamele edildi\u011finde, h\u00fccre hayatta kalma oran\u0131 60.64% olmu\u015ftur. Bu s\u0131rada PC12 h\u00fccreleri belirli bir \u00f6l\u00e7\u00fcde hasar g\u00f6rm\u00fc\u015f, ancak hen\u00fcz geri d\u00f6nd\u00fcr\u00fclemez bir duruma ula\u015fmam\u0131\u015ft\u0131. Bu nedenle, sonraki deneylerde PC12 h\u00fccrelerinde 4 saat boyunca oksidatif hasar i\u00e7in optimum konsantrasyon ve zaman olarak 300 \u03bc mol\/L se\u00e7ilmi\u015ftir.<\/p>\n

BRNP'nin PC12 h\u00fccreleri \u00fczerinde sitotoksik etkileri olup olmad\u0131\u011f\u0131n\u0131 do\u011frulamak i\u00e7in BRNP, H2O2 hasar\u0131 olmaks\u0131z\u0131n PC12 h\u00fccrelerini tedavi etmek i\u00e7in tek ba\u015f\u0131na kullan\u0131lm\u0131\u015ft\u0131r. Deneysel sonu\u00e7lar, BRNP'nin farkl\u0131 konsantrasyonlar\u0131 (7.8-2000 \u03bcg\/mL) ile muamele edildikten sonra PC12 h\u00fccrelerinin hayatta kalma oran\u0131nda \u00f6nemli bir de\u011fi\u015fiklik olmad\u0131\u011f\u0131n\u0131 g\u00f6stermi\u015ftir (P>0.05), bu da BRNP'nin PC12 h\u00fccre canl\u0131l\u0131\u011f\u0131 \u00fczerinde \u00f6nemli bir sitotoksisitesi olmad\u0131\u011f\u0131n\u0131 g\u00f6stermektedir. BRNP'nin PC12 h\u00fccrelerinde H2O2 kaynakl\u0131 hasar \u00fczerindeki etkisi incelendi\u011finde, sonu\u00e7lar Model grubu ile kar\u015f\u0131la\u015ft\u0131r\u0131ld\u0131\u011f\u0131nda, h\u00fccre hayatta kalma oran\u0131n\u0131n BRNP konsantrasyonunun artmas\u0131yla kademeli olarak artt\u0131\u011f\u0131n\u0131 (P<0.05) ve BRNP konsantrasyonu 250 \u03bcg\/mL'ye ula\u015ft\u0131\u011f\u0131nda h\u00fccre hayatta kalma oran\u0131n\u0131n \u00f6nemli \u00f6l\u00e7\u00fcde artt\u0131\u011f\u0131n\u0131 g\u00f6stermi\u015ftir. Deneysel sonu\u00e7lara g\u00f6re BRNP, PC12 h\u00fccrelerinde H2O2 kaynakl\u0131 hasar \u00fczerinde belirli bir koruyucu etkiye sahiptir.
\nLaktat dehidrojenaz (LDH) h\u00fccrelerin i\u00e7inde bulunur ve piruvattan laktat \u00fcretimini katalize edebilir. H\u00fccreler hasar g\u00f6rd\u00fc\u011f\u00fcnde, LDH b\u00fcy\u00fck miktarlarda sal\u0131n\u0131r ve h\u00fccre d\u0131\u015f\u0131 LDH seviyelerinde \u00f6nemli bir art\u0131\u015fa neden olur. Sal\u0131nan LDH miktar\u0131 hasar\u0131n derecesiyle orant\u0131l\u0131d\u0131r, bu nedenle LDH s\u0131z\u0131nt\u0131 oran\u0131 h\u00fccre hasar\u0131n\u0131n derecesini yans\u0131tabilir. Bu deneyin sonu\u00e7lar\u0131 BRNP'nin h\u00fccre zar\u0131 hasar\u0131n\u0131n derecesini \u00f6nleyebilece\u011fini veya yava\u015flatabilece\u011fini ve h\u00fccre i\u00e7i LDH s\u0131z\u0131nt\u0131s\u0131n\u0131 \u00f6nleyebilece\u011fini g\u00f6stermektedir.
\nH\u00fccre i\u00e7indeki mitokondrilerin i\u00e7 zar\u0131n\u0131n her iki taraf\u0131ndaki proton da\u011f\u0131l\u0131m\u0131 tekd\u00fcze de\u011fildir ve mitokondriyal zar potansiyeli olarak bilinen elektrokimyasal bir gradyan olu\u015fturur. \u00c7ok say\u0131da \u00e7al\u0131\u015fma, herhangi bir apoptoz ind\u00fckleyici fakt\u00f6r h\u00fccre apoptozunu uyard\u0131\u011f\u0131nda, mitokondriyal membran potansiyelinde bir d\u00fc\u015f\u00fc\u015f meydana geldi\u011fini ve membran potansiyelindeki bu d\u00fc\u015f\u00fc\u015f\u00fcn h\u00fccre morfolojisindeki de\u011fi\u015fikliklerden daha \u00f6nce meydana geldi\u011fini g\u00f6stermi\u015ftir. Mitokondriyal membran potansiyelindeki \u00f6nemli d\u00fc\u015f\u00fc\u015f, h\u00fccrelerin geri d\u00f6n\u00fc\u015f\u00fcms\u00fcz apoptozuna yol a\u00e7acakt\u0131r, bu nedenle mitokondriyal membran potansiyelindeki d\u00fc\u015f\u00fc\u015f, h\u00fccre apoptozunun erken evresinde klasik bir fenomen olarak kabul edilebilir. G\u00fcn\u00fcm\u00fczde ara\u015ft\u0131rmalar, mitokondriyal i\u015flev bozuklu\u011fu ile ya\u015flanma s\u00fcreci aras\u0131nda yak\u0131n bir ili\u015fki oldu\u011funu do\u011frulam\u0131\u015ft\u0131r. \u00d6rne\u011fin, mitokondriyal enerjinin (MMP ve ATP) yetersiz tedariki v\u00fccudun metabolik kapasitesini azaltarak bir dizi ya\u015flanma de\u011fi\u015fikli\u011fine yol a\u00e7abilir. Bunlar aras\u0131nda, mitokondriyal membran potansiyelindeki azalma, h\u00fccre apoptozunun spesifik belirte\u00e7lerinden biridir. Ara\u015ft\u0131rma sonu\u00e7lar\u0131, PC12 h\u00fccrelerinin mitokondriyal membran potansiyelinin H2O2 tedavisinden sonra \u00f6nemli \u00f6l\u00e7\u00fcde azald\u0131\u011f\u0131n\u0131 ve BRNP \u00f6n tedavisinin H2O2'nin neden oldu\u011fu membran potansiyeli azalmas\u0131n\u0131 \u00f6nleyebilece\u011fini g\u00f6stermi\u015ftir.
\nApoptoz, eksojen Fas\/FasL arac\u0131l\u0131 \u00f6l\u00fcm resept\u00f6r\u00fc yollar\u0131n\u0131n yan\u0131 s\u0131ra endojen mitokondriyal ve endoplazmik retikulum yollar\u0131n\u0131 da i\u00e7eren programlanm\u0131\u015f bir h\u00fccre \u00f6l\u00fcm\u00fc s\u00fcrecidir. Bunlar aras\u0131nda, mitokondriyal arac\u0131l\u0131 endojen apoptoz yolu, memeli h\u00fccrelerinde programlanm\u0131\u015f h\u00fccre \u00f6l\u00fcm\u00fc i\u00e7in ana yoldur. Pro apoptotik proteinler taraf\u0131ndan temsil edilen Bax ve anti apoptotik proteinler taraf\u0131ndan temsil edilen Bcl-2, mitokondriyal apoptoz fakt\u00f6rlerinin sal\u0131n\u0131m\u0131n\u0131 d\u00fczenleyen ana proteinlerdir. Caspase proteaz memelilerde anahtar bir apoptoz efekt\u00f6r\u00fcd\u00fcr, bunlar\u0131n aras\u0131nda Caspase-3 \u00e7e\u015fitli apoptoz uyar\u0131c\u0131 fakt\u00f6rleri aktive eden ve h\u00fccre apoptozunun \u00f6nemli bir uygulay\u0131c\u0131s\u0131 olarak bilinen Caspase ailesindeki anahtar bir proteazd\u0131r. BRNP, H2O2 oksidasyonu ile hasar g\u00f6ren PC12 h\u00fccrelerinde Kaspaz-3 ekspresyonunu belirli bir \u00f6l\u00e7\u00fcde azalt\u0131r. BRNP'nin Kaspaz-3 ekspresyonunu azaltabilece\u011fi, h\u00fccre apoptozunu azaltabilece\u011fi ve b\u00f6ylece v\u00fccudun ya\u015flanmas\u0131n\u0131 geciktirebilece\u011fi tahmin edilebilir. BRNP ile m\u00fcdahaleden sonra, oksidatif hasarl\u0131 PC12 h\u00fccrelerinde Bcl-2 ekspresyon seviyesi artarken, Bax ve BRNP ekspresyon seviyeleri de\u011fi\u015fen derecelerde azalm\u0131\u015ft\u0131r. Bu ara\u015ft\u0131rma sonu\u00e7lar\u0131, BRNP'nin h\u00fccrelerin oksidatif stresin mitokondri ve DNA'ya verdi\u011fi hasara direnmesine yard\u0131mc\u0131 oldu\u011funu, h\u00fccresel oksidatif hasar\u0131 azaltt\u0131\u011f\u0131n\u0131 ve v\u00fccudun antioksidan stresi d\u00fczenleme yetene\u011fini art\u0131rd\u0131\u011f\u0131n\u0131 g\u00f6sterebilir.<\/p>","protected":false},"excerpt":{"rendered":"

Preliminary study on the protective mechanism of turnip neutral polysaccharides against oxidative damage in PC12 cells Turnip (Brassica rapa L.), also known as Chagu or Manjing, belongs to the Brassicaceae family and the Brassica genus. It is used as a medicinal herb with its roots and is rich in nutrients. It can be consumed for […]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"\nPreliminary study on the protective mechanism of turnip neutral polysaccharides against oxidative damage in PC12 cells - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/tr\/salgam-notral-polisakkaritleri\/\" \/>\n<meta property=\"og:locale\" content=\"tr_TR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Preliminary study on the protective mechanism of turnip neutral polysaccharides against oxidative damage in PC12 cells - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"Preliminary study on the protective mechanism of turnip neutral polysaccharides against oxidative damage in PC12 cells Turnip (Brassica rapa L.), also known as Chagu or Manjing, belongs to the Brassicaceae family and the Brassica genus. It is used as a medicinal herb with its roots and is rich in nutrients. 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It is used as a medicinal herb with its roots and is rich in nutrients. 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