{"id":6642,"date":"2024-08-06T12:49:34","date_gmt":"2024-08-06T12:49:34","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=6642"},"modified":"2024-08-06T12:49:34","modified_gmt":"2024-08-06T12:49:34","slug":"primitive-leeches","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/tr\/primitive-leeches\/","title":{"rendered":"Farkl\u0131 \u0130lkel S\u00fcl\u00fcklerin ve Kar\u0131\u015f\u0131k \u00dcr\u00fcnlerinin Kalitesi \u00dczerine \u00c7al\u0131\u015fma"},"content":{"rendered":"

Farkl\u0131 \u0130lkel S\u00fcl\u00fcklerin ve Kar\u0131\u015f\u0131k \u00dcr\u00fcnlerinin Kalitesi \u00dczerine \u00c7al\u0131\u015fma
\nBundan b\u00f6yle \u00c7in Farmakopesi olarak an\u0131lacak olan \u00c7in Halk Cumhuriyeti Farmakopesi'nin 2020 bask\u0131s\u0131, s\u00fcl\u00fcklerin Whitman pigra Whitman s\u00fcl\u00fc\u011f\u00fc, Hirudo nipponica Whitman s\u00fcl\u00fc\u011f\u00fc veya s\u00f6\u011f\u00fct yapra\u011f\u0131 s\u00fcl\u00fc\u011f\u00fc Whitman acranulata Whitman gibi s\u00fcl\u00fck ailesi hayvanlar\u0131n\u0131n kurutulmu\u015f b\u00fct\u00fcn\u00fc oldu\u011funu belirtmektedir. Tad\u0131 tuzlu, ac\u0131 ve d\u00fczd\u00fcr; karaci\u011fer meridyenine ait k\u00fc\u00e7\u00fck bir toksin vard\u0131r. Kan\u0131 k\u0131rma ve dola\u015f\u0131m\u0131 te\u015fvik etme, kan staz\u0131n\u0131 giderme ve durgunlu\u011fu ortadan kald\u0131rma i\u015flevine sahiptir. Genellikle kan staz\u0131 ve meridyen t\u0131kan\u0131kl\u0131\u011f\u0131, kitlelerin tedavisi, inme hemiplejisi ve d\u00fc\u015fme ve yaralanmalar i\u00e7in kullan\u0131l\u0131r. \u00c7al\u0131\u015fmalar, s\u00fcl\u00fcklerin farmakolojik etkilerinin esas olarak antikoag\u00fclasyon, antitrombotik, anti-t\u00fcm\u00f6r, hipolipidemik ve anti-inflamatuar etkileri i\u00e7erdi\u011fini ve tromboz, hiperlipidemi, hipertansiyon, diyabet, prostatit, kanser, varisli damarlar, k\u0131s\u0131rl\u0131k, siroz ve di\u011fer hastal\u0131klar\u0131n tedavisi dahil olmak \u00fczere \u00e7ok geni\u015f klinik uygulamalara sahip oldu\u011funu g\u00f6stermi\u015ftir. S\u00fcl\u00fcklerin ana bile\u015fenleri, esas olarak proteinler ve insan v\u00fccudu i\u00e7in 8 temel amino asit dahil olmak \u00fczere 17 tip amino asit i\u00e7eren makromolek\u00fcler bile\u015fiklerdir. Hidrolize amino asit i\u00e7eri\u011fi 49.4% kadar y\u00fcksektir; ayr\u0131ca hirudin, heparin, histamin, hirudin, glikolipidler, sfenoidler, steroidler ve karboksilik esterler gibi \u00e7e\u015fitli maddeler de i\u00e7erir.
\nLiterat\u00fcr taramas\u0131 sonucunda, s\u00fcl\u00fckler \u00fczerine yap\u0131lan mevcut ara\u015ft\u0131rmalar\u0131n antikoag\u00fclan mekanizmalar ve amino asit tayini \u00fczerine odakland\u0131\u011f\u0131, farkl\u0131 ilkel s\u00fcl\u00fcklerin ve kar\u0131\u015f\u0131mlar\u0131n\u0131n kalitesi \u00fczerine kapsaml\u0131 \u00e7al\u0131\u015fmalar\u0131n s\u0131n\u0131rl\u0131 oldu\u011fu tespit edilmi\u015ftir. Farkl\u0131 ilkel s\u00fcl\u00fckler ve kar\u0131\u015f\u0131mlar\u0131 aras\u0131ndaki tan\u0131mlama \u00e7al\u0131\u015fmalar\u0131 \u00e7o\u011funlukla \u00c7in Farmakopesi'nin 2020 bask\u0131s\u0131 kapsam\u0131ndaki \u00f6zellik tan\u0131mlamas\u0131na dayanmaktad\u0131r. S\u00fcl\u00fck pazar\u0131 pahal\u0131 ve kaotiktir ve geleneksel g\u00f6r\u00fcn\u00fcm tan\u0131mlamas\u0131 tek ba\u015f\u0131na kalitelerini h\u0131zl\u0131 ve etkili bir \u015fekilde de\u011ferlendiremez. Dahas\u0131, g\u00f6r\u00fcn\u00fcm tan\u0131mlamas\u0131, belirli s\u0131n\u0131rlamalar\u0131 olan s\u00fcl\u00fck \u00f6zlerine ve preparatlar\u0131na uygulanamaz. Bu nedenle, h\u0131zl\u0131, bilimsel ve etkili bir de\u011ferlendirme y\u00f6nteminin olu\u015fturulmas\u0131 b\u00fcy\u00fck \u00f6nem ta\u015f\u0131maktad\u0131r. Bu \u00e7al\u0131\u015fmada, s\u00fcl\u00fcklerin karakteristik spektrumunu ve i\u00e7erik belirleme y\u00f6ntemini olu\u015fturmak i\u00e7in HPLC kullan\u0131lm\u0131\u015ft\u0131r. Bu y\u00f6ntem, s\u00fcl\u00fckleri ve kar\u0131\u015f\u0131k \u00fcr\u00fcnlerini kapsaml\u0131 bir \u015fekilde de\u011ferlendirerek, s\u00fcl\u00fcklerin ve kar\u0131\u015f\u0131k \u00fcr\u00fcnlerinin kalite de\u011ferlendirmesi i\u00e7in bilimsel bir temel sa\u011flar.<\/p>\n

\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"
\nKromatografik ko\u015fullar\u0131n optimizasyonu. Karakteristik bir spektrum olu\u015fturma temelinde, bu \u00e7al\u0131\u015fmada s\u00fcl\u00fc\u011f\u00fcn iki karakteristik bile\u015feni olan s\u00fcl\u00fck amin C karboksil t\u00fcrevi ve s\u00fcl\u00fck amin B'nin i\u00e7eri\u011fini belirlemek i\u00e7in ayn\u0131 y\u00f6ntem kullan\u0131lm\u0131\u015ft\u0131r. 200-400nm dalga boyu aral\u0131\u011f\u0131nda test \u00e7\u00f6zeltisinin tam dalga boyu taramas\u0131n\u0131 ger\u00e7ekle\u015ftirmek i\u00e7in bir diyot dizi dedekt\u00f6r\u00fc kullan\u0131lm\u0131\u015ft\u0131r. Sonu\u00e7lar, her bir ana bile\u015fenin kromatografik piklerinin 254nm dalga boyunda g\u00fc\u00e7l\u00fc UV absorpsiyonuna sahip oldu\u011funu g\u00f6stermi\u015ftir. Bu nedenle, s\u00fcl\u00fck \u00f6rneklerinin karakteristik spektrumunu ve i\u00e7eri\u011fini analiz etmek i\u00e7in ayn\u0131 ko\u015fullar kullan\u0131lm\u0131\u015ft\u0131r.
\n\u00d6zellik haritas\u0131 sonu\u00e7lar\u0131n\u0131n analizi. S\u00fcl\u00fck, s\u00fcl\u00fckgiller familyas\u0131ndan bir hayvan olan Whitmania pigra Whitman s\u00fcl\u00fc\u011f\u00fcn\u00fcn kurutulmu\u015f b\u00fct\u00fcn g\u00f6vdesidir. T\u0131bbi \u00f6zellikleri d\u00fcz i\u011f \u015feklindedir. \u00c7in Farmakopesi'nin 2020 bask\u0131s\u0131 kalitesini yaln\u0131zca nem, toplam k\u00fcl i\u00e7eri\u011fi, asitlik ve alkalinite testleri ve antitrombin aktivitesi ile kontrol etmektedir ve hen\u00fcz farkl\u0131 substratlar aras\u0131nda ay\u0131r\u0131c\u0131 karakterizasyon yapmam\u0131\u015ft\u0131r. Bu \u00e7al\u0131\u015fmada, farkl\u0131 k\u00f6kenlere sahip hirudo ve ta\u011f\u015fi\u015flerinin HPLC karakteristik haritalar\u0131 olu\u015fturulmu\u015ftur. Referans maddelerin kar\u015f\u0131la\u015ft\u0131r\u0131lmas\u0131yla hirudo'nun be\u015f bile\u015fen i\u00e7erdi\u011fi do\u011frulanm\u0131\u015ft\u0131r: urasil, hipoksantin, ksantin, hirudin C-karboksil t\u00fcrevleri, hirudin B. Literat\u00fcr raporlar\u0131na g\u00f6re, yukar\u0131daki pteridin bile\u015fikleri klopidogrel gibi antiplatelet agregasyon ila\u00e7lar\u0131yla benzer yap\u0131lara sahiptir ve bu bile\u015fenlerin antiplatelet agregasyonunu oynamak i\u00e7in hirudo'nun aktif bile\u015fenleri olabilece\u011fine inan\u0131lmaktad\u0131r. Hirudo'nun bildirilen farmakolojik etkileri daha \u00e7ok antikoag\u00fclan, antitrombotik ve antiaterosklerotik etkilerdir. Buna ek olarak, anti-t\u00fcm\u00f6r, anti inflamatuar, anti fibrozis ve lipidemik aktiviteler vard\u0131r, bu da bu \u00e7al\u0131\u015fmada se\u00e7ilen karakteristik zirvelerin Farmakolojik aktiviteleri oynayan s\u00fcl\u00fcklerin fonksiyonel maddelerini a\u00e7\u0131kl\u0131\u011fa kavu\u015fturmak ve ila\u00e7lar\u0131n ikincil ara\u015ft\u0131rma ve geli\u015ftirmesini te\u015fvik etmek b\u00fcy\u00fck \u00f6nem ta\u015f\u0131maktad\u0131r. Ayn\u0131 zamanda, olu\u015fturulan y\u00f6ntem farkl\u0131 ilkel s\u00fcl\u00fckleri ve bunlar\u0131n kar\u0131\u015f\u0131k \u00fcr\u00fcnlerini tan\u0131mlayabilir. Bir sonraki ad\u0131m, bu y\u00f6ntemin ekstrakt ve preparatlarda uygulama ara\u015ft\u0131rmas\u0131na devam etmek ve s\u00fcl\u00fck t\u0131bbi malzemelerinin geli\u015ftirilmesi ve kullan\u0131m\u0131 i\u00e7in referans sa\u011flamakt\u0131r.
\n\u0130\u00e7erik belirleme sonu\u00e7lar\u0131n\u0131n analizi. Karakteristik haritalar\u0131n incelenmesine dayanan bu \u00e7al\u0131\u015fma, s\u00fcl\u00fcklerde \u00fc\u00e7 n\u00fckleozit bile\u015fi\u011fi, urasil, hipoksantin ve iki sfenoid bile\u015fi\u011fi, leechamine C-karboksil t\u00fcrevleri ve leechamine B dahil olmak \u00fczere be\u015f bile\u015feni kantitatif olarak belirlemi\u015ftir. Mevcut literat\u00fcr raporlar\u0131na g\u00f6re, n\u00fckleozit bile\u015fikleri ve sfenoid bile\u015fikleri s\u00fcl\u00fckler i\u00e7in kalite de\u011ferlendirme g\u00f6stergeleri olarak kullan\u0131labilir. Deneysel sonu\u00e7lar, farkl\u0131 ilkel s\u00fcl\u00fckler ve bunlar\u0131n kar\u0131\u015f\u0131mlar\u0131 aras\u0131nda urasil ve hipoksantin i\u00e7eri\u011finde belirli farkl\u0131l\u0131klar oldu\u011funu g\u00f6stermi\u015ftir. Ortalama urasil ve hipoksantin i\u00e7eri\u011fi \u015fu s\u0131radayd\u0131: s\u00fcl\u00fck>s\u00fcl\u00fck>s\u00f6\u011f\u00fct s\u00fcl\u00fc\u011f\u00fc>Philippine s\u00fcl\u00fc\u011f\u00fc; Ortalama ksantin i\u00e7eri\u011fi a\u015fa\u011f\u0131daki gibidir: s\u00fcl\u00fck>s\u00fcl\u00fck>Filipin s\u00fcl\u00fc\u011f\u00fc>s\u00f6\u011f\u00fct yapra\u011f\u0131 s\u00fcl\u00fc\u011f\u00fc; S\u00fcl\u00fck amininin C-karboksil t\u00fcrevlerinin ortalama i\u00e7eri\u011fi \u015fu \u015fekildedir: yaprak s\u00fcl\u00fc\u011f\u00fc>s\u00fcl\u00fck>s\u00fcl\u00fck>Filipin s\u00fcl\u00fc\u011f\u00fc; S\u00fcl\u00fckamin B'nin ortalama i\u00e7eri\u011fi \u015fu \u015fekildedir: yaprak s\u00fcl\u00fc\u011f\u00fc>s\u00fcl\u00fck>s\u00fcl\u00fck>Filipin s\u00fcl\u00fc\u011f\u00fc. Filipin s\u00fcl\u00fc\u011f\u00fc s\u00fcl\u00fckamin B i\u00e7ermez ve hipoksantin i\u00e7eri\u011fi di\u011fer \u00fc\u00e7 substrattan \u00f6nemli \u00f6l\u00e7\u00fcde daha d\u00fc\u015f\u00fckt\u00fcr. Bu nedenle, yukar\u0131daki bile\u015fenlerin i\u00e7eri\u011finin belirlenmesi, su s\u00fcl\u00fckleri (s\u00fcl\u00fckler, s\u00fcl\u00fckler ve yaprak s\u00fcl\u00fckleri dahil) ve t\u0131bbi s\u00fcl\u00fckler (Filipin s\u00fcl\u00fckleri) aras\u0131nda ayr\u0131m yapmak i\u00e7in kullan\u0131labilir; Ayr\u0131ca PLS-DA analizi, numunedeki 8 karakteristik pikin pik alanlar\u0131n\u0131 de\u011fi\u015fken olarak kullan\u0131r ve karakteristik piklerin pik alanlar\u0131, kar\u015f\u0131l\u0131k gelen g\u00f6sterge bile\u015fenlerinin i\u00e7eri\u011fi ile yak\u0131ndan ili\u015fkilidir. \"2.4.5 \"teki PLS-DA analiz sonu\u00e7lar\u0131 ile birle\u015ftirildi\u011finde, hipoksantin ve ksantine kar\u015f\u0131l\u0131k gelen karakteristik piklerin VIP de\u011ferleri 1'den b\u00fcy\u00fckt\u00fcr ve s\u00f6\u011f\u00fct yapra\u011f\u0131 s\u00fcl\u00fc\u011f\u00fc ksantin i\u00e7ermez. Di\u011fer \u00fc\u00e7 ilkel ksantinin ortalama i\u00e7eri\u011fi de \u00f6nemli farkl\u0131l\u0131klar g\u00f6stermekte, bu da bu be\u015f bile\u015fenin i\u00e7erik belirleme g\u00f6stergeleri olarak se\u00e7ilmesinin belirli bir bilimsel ge\u00e7erlili\u011fe sahip oldu\u011funu g\u00f6stermektedir.
\nAra\u015ft\u0131rma enstit\u00fcm\u00fcz taraf\u0131ndan olu\u015fturulan y\u00f6ntem do\u011fru, hassas ve iyi bir \u00f6zg\u00fcll\u00fc\u011fe sahiptir. Karakteristik spektrumlar\u0131 e\u015f zamanl\u0131 olarak analiz edebilir ve s\u00fcl\u00fck t\u0131bbi malzemelerinin i\u00e7eri\u011fini de\u011ferlendirebilir. Farkl\u0131 ilkel s\u00fcl\u00fckler ve bunlar\u0131n kar\u0131\u015f\u0131mlar\u0131 i\u00e7in bir kalite de\u011ferlendirme y\u00f6ntemi olarak kullan\u0131labilir ve s\u00fcl\u00fck t\u0131bbi malzemelerinin kalite de\u011ferlendirmesi i\u00e7in bir referans sa\u011flar.<\/p>","protected":false},"excerpt":{"rendered":"

Study on the Quality of Different Primitive Leeches and Their Confused Products The 2020 edition of the Pharmacopoeia of the People’s Republic of China, hereinafter referred to as the Chinese Pharmacopoeia, stipulates that leeches are the dried whole of the leech family animals, such as the leech Whitman pigra Whitman, leech Hirudo nipponica Whitman, or […]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"\nStudy on the Quality of Different Primitive Leeches and Their Confused Products - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/tr\/primitive-leeches\/\" \/>\n<meta property=\"og:locale\" content=\"tr_TR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Study on the Quality of Different Primitive Leeches and Their Confused Products - 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