{"id":6627,"date":"2024-08-06T12:47:33","date_gmt":"2024-08-06T12:47:33","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=6627"},"modified":"2024-08-06T12:47:33","modified_gmt":"2024-08-06T12:47:33","slug":"xihuangcao-medicinal-herbs","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/tr\/xihuangcao-medicinal-herbs\/","title":{"rendered":"Xihuangcao \u015fifal\u0131 otlar\u0131n\u0131n d\u00f6rt orijinal bitkisinin UPLC karakteristik spektrumlar\u0131n\u0131n tan\u0131mlanmas\u0131"},"content":{"rendered":"

Xihuangcao \u015fifal\u0131 otlar\u0131n\u0131n d\u00f6rt orijinal bitkisinin UPLC karakteristik spektrumlar\u0131n\u0131n tan\u0131mlanmas\u0131
\nXihuang Otu, Be\u015f S\u0131rt\u0131n g\u00fcneyinde, \u0131s\u0131y\u0131 temizleme ve rutubeti giderme, kan\u0131 so\u011futma ve staz\u0131 giderme etkisine sahip e\u015fsiz bir \u00c7in bitkisel ilac\u0131d\u0131r. Nemli \u0131s\u0131 sar\u0131l\u0131\u011f\u0131, abdominal distansiyon ve hipokondriyak a\u011fr\u0131, nemli \u0131s\u0131 ishali, \u0131s\u0131 toksini ishali ve travmatik yaralanmalar i\u00e7in kullan\u0131l\u0131r. Be\u015f S\u0131rt\u0131n g\u00fcneyinde yayg\u0131n olarak kullan\u0131lan bir klinik ila\u00e7t\u0131r ve ayn\u0131 zamanda \u00fcnl\u00fc geleneksel \u00c7in patentli ila\u00e7lar\u0131n ve basit preparatlar\u0131n b\u00fcy\u00fck \u00e7e\u015fitlilikteki \"anti-enflamatuar ve cholagogue tabletlerinin\" re\u00e7etesi i\u00e7in bir hammaddedir. \u00c7in Farmakopesi (2020 bask\u0131s\u0131, Cilt 4), Labiatae familyas\u0131na ve Rabdosia cinsine ait bir bitki olan Rabdosia lophanthoides (Buch. - Ham. ex D. DON) H. Hara veya Rabdosia serora (Maxim.) H. Hrar'\u0131n kurutulmu\u015f toprak \u00fcst\u00fc k\u0131sm\u0131 olarak kaydeder. Guangdong Eyaleti \u00c7in Bitkisel \u0130la\u00e7 Standartlar\u0131nda yer alan orijinal bitki Rabdosia lophanthoides R serra\u3001 \u0130plik desenli kokulu \u00e7ay ve sebze R Lophanthoides ve lifli \u00e7i\u00e7ek \u00e7e\u015fidi R lophanthoides var. graciliflora(Benth.) H. Hrar\u3002 Ara\u015ft\u0131rma grubumuzun \u00fcyeleri, Xihuangcao t\u0131bbi malzemelerinin germplazm kaynaklar\u0131n\u0131 ara\u015ft\u0131rma ve d\u00fczenleme s\u00fcrecinde, ayn\u0131 cinse ait bir bitki olan Rabdosia stracheyi (Benth. ex Hook. f.) Hara'n\u0131n da halk k\u00fclt\u00fcr\u00fcnde Xihuangcao t\u0131bbi malzemeleri olarak yayg\u0131n bir \u015fekilde kullan\u0131ld\u0131\u011f\u0131n\u0131 ke\u015ffetti. Bu bitkilerin benzer morfolojik \u00f6zellikleri nedeniyle, kuru t\u0131bbi malzemeleri g\u00f6r\u00fcn\u00fcmlerine g\u00f6re ay\u0131rt etmek zordur ve Xihuangcao ticari t\u0131bbi malzemelerinin \u00e7e\u015fit kaynaklar\u0131nda her zaman bir kar\u0131\u015f\u0131kl\u0131k olgusu olmu\u015ftur. Bu nedenle, klinik ila\u00e7 g\u00fcvenli\u011fini ve etkinli\u011fini sa\u011flamak ve geli\u015ftirmek i\u00e7in farkl\u0131 Huangcao t\u00fcrleri i\u00e7in tan\u0131mlama y\u00f6ntemleri olu\u015fturmak b\u00fcy\u00fck \u00f6nem ta\u015f\u0131maktad\u0131r.<\/p>\n

Geleneksel olarak, Xihuangcao t\u0131bbi malzemelerinin farkl\u0131 orijinal bitkilerinin tan\u0131mlanmas\u0131 morfoloji ve mikroskopi a\u00e7\u0131s\u0131ndan incelenmi\u015ftir. Bununla birlikte, kuru t\u0131bbi malzemelerin genellikle buru\u015fuk ve k\u0131r\u0131k olmas\u0131 nedeniyle, bunlar\u0131 do\u011fru ve h\u0131zl\u0131 bir \u015fekilde tan\u0131mlamak zordur. Son y\u0131llarda akademisyenler orijinal bitkiler \u00fczerinde DNA molek\u00fclleri ve kimyasal bile\u015fenler d\u00fczeyinde tan\u0131mlama \u00e7al\u0131\u015fmalar\u0131 y\u00fcr\u00fctm\u00fc\u015ft\u00fcr. Ancak, molek\u00fcler tan\u0131mlama Rabdosia serora ve Lycaena angustifolia aras\u0131nda yaln\u0131zca t\u00fcr d\u00fczeyinde ayr\u0131m yapabilmektedir ve bu da Lycaena angustifolia'n\u0131n orijinal ve varyant \u00e7e\u015fitlerini ay\u0131rt etmeyi zorla\u015ft\u0131rmaktad\u0131r. Kimyasal bile\u015fim ara\u015ft\u0131rmas\u0131 a\u00e7\u0131s\u0131ndan, baz\u0131 akademisyenler Fourier d\u00f6n\u00fc\u015f\u00fcml\u00fc k\u0131z\u0131l\u00f6tesi spektroskopisi ve iki boyutlu korelasyon k\u0131z\u0131l\u00f6tesi spektroskopisinin Xihuangcao, \u00c7izgili Kokulu \u00c7ay Sebzesi ve lifli \u00e7i\u00e7ekleri ile dar taban \u00e7e\u015fitlerinin tan\u0131mlanmas\u0131n\u0131 sa\u011flayabilece\u011fine inanmaktad\u0131r. Baz\u0131 akademisyenler de kimyasal bile\u015fim i\u00e7eri\u011fi ve parmak izi spektrumlar\u0131n\u0131n tan\u0131mlanmas\u0131 \u00fczerine ara\u015ft\u0131rmalar yapm\u0131\u015flard\u0131r, ancak deneysel sonu\u00e7lar \u00c7izgili Kokulu \u00c7ay Sebzesinin orijinal \u00e7e\u015fidini varyantlar\u0131ndan \u00e7ok iyi ay\u0131rt edememektedir. Son y\u0131llarda, kemometrik y\u00f6ntemler geleneksel \u00c7in t\u0131bb\u0131n\u0131n kromatografik analizinde giderek daha yayg\u0131n bir \u015fekilde kullan\u0131lmaktad\u0131r. Kromatografik analizle \u00fcretilen b\u00fcy\u00fck miktarda veriyi daha fazla analiz edebilir ve i\u015fleyebilirler ve kromatografik analizle birle\u015ftirildiklerinde geleneksel \u00c7in t\u0131bb\u0131n\u0131n kalite kontrol\u00fcnde giderek daha \u00f6nemli bir rol oynarlar. Ultra y\u00fcksek performansl\u0131 s\u0131v\u0131 kromatografisi (UPLC) k\u0131sa analiz s\u00fcresi, iyi ay\u0131rma etkisi ve y\u00fcksek hassasiyet avantajlar\u0131na sahiptir. Bu nedenle, bu \u00e7al\u0131\u015fmada Xihuangcao, Xianwen Xiangchacai, Xianxiang Xiangchacai ve Longye Xiangchacai olmak \u00fczere d\u00f6rt farkl\u0131 temel bitkiden toplanan 32 parti t\u0131bbi malzemenin karakteristik spektrumlar\u0131n\u0131 incelemek i\u00e7in UPLC kullan\u0131lm\u0131\u015f ve Xixi i\u00e7in de\u011ferli bilgiler sa\u011flamak amac\u0131yla bu d\u00f6rt temel bitkiyi ay\u0131rt etmek ve tan\u0131mlamak i\u00e7in k\u00fcme analizi, temel bile\u015fen analizi ve k\u0131smi en k\u00fc\u00e7\u00fck kareler diskriminant analizi gibi kemometrik y\u00f6ntemlerle birle\u015ftirilmi\u015ftir. Huangcao t\u0131bbi malzemelerinin tan\u0131mlanmas\u0131 ve kalitelerinin analizi ve de\u011ferlendirilmesi i\u00e7in referans sa\u011flar.<\/p>\n

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Bu \u00e7al\u0131\u015fmada, test \u00e7\u00f6zeltisinin haz\u0131rlama y\u00f6ntemleri ve kromatografik ko\u015fullar\u0131 taranm\u0131\u015f ve optimize edilmi\u015ftir. Ekstraksiyon \u00e7\u00f6z\u00fcc\u00fclerinin (metanol, 80% metanol, 60% metanol) ara\u015ft\u0131rma sonu\u00e7lar\u0131, ekstraksiyon \u00e7\u00f6z\u00fcc\u00fcs\u00fc olarak metanol kullan\u0131ld\u0131\u011f\u0131nda, elde edilen numune kromatogram\u0131n\u0131n daha fazla kromatografik pike, nispeten daha b\u00fcy\u00fck pik alanlar\u0131na ve Xihuangcao t\u0131bbi malzemesinin kimyasal bile\u015fimini daha iyi yans\u0131tan sabit bir taban \u00e7izgisine sahip oldu\u011funu g\u00f6stermi\u015ftir. Bu nedenle, Xihuangcao t\u0131bbi malzemesinin karakteristik spektrumu i\u00e7in ekstraksiyon \u00e7\u00f6z\u00fcc\u00fcs\u00fc olarak metanol se\u00e7ildi; Dalga boylar\u0131n\u0131n (250, 254, 260, 280nm) \u00f6l\u00e7\u00fcm sonu\u00e7lar\u0131, 254nm'de bir\u00e7ok kromatografik pik oldu\u011funu ve her pikin boyutlar\u0131n\u0131n tek tip oldu\u011funu g\u00f6sterdi. Bu nedenle, 254nm tespit dalga boyu olarak se\u00e7ilmi\u015ftir; Mobil fazlar\u0131n (metanol -0.1% formik asit su, asetonitril -0.1% formik asit su, 0.1% formik asit metanol -0.1% formik asit su ve 0.1% formik asit asetonitril -0.1% formik asit su) ara\u015ft\u0131rma sonu\u00e7lar\u0131, metanol -0.El\u00fcsyon i\u00e7in mobil faz olarak 1% formik asit suyu kullan\u0131ld\u0131\u011f\u0131nda, taban \u00e7izgisinin d\u00fcz oldu\u011fu ve ay\u0131rma derecesi ile pik \u015feklinin iyi oldu\u011fu; Kolon s\u0131cakl\u0131\u011f\u0131 ve ak\u0131\u015f h\u0131z\u0131 ara\u015ft\u0131rmas\u0131n\u0131n sonu\u00e7lar\u0131, her bir kromatografik y\u00f6ntemin ay\u0131rma verimlili\u011finin, kolon s\u0131cakl\u0131\u011f\u0131 30 \u2103 ve ak\u0131\u015f h\u0131z\u0131 0,3 mL \/ dak oldu\u011funda en iyi oldu\u011funu g\u00f6stermi\u015ftir.<\/p>\n

Bu \u00e7al\u0131\u015fmada, Xihuangcao \u015fifal\u0131 bitkisinin d\u00f6rt temel bitkisinin tan\u0131mlanmas\u0131na uygulanabilir bir karakteristik harita olu\u015fturmak i\u00e7in UPLC kullan\u0131lm\u0131\u015ft\u0131r. Taban \u00e7izgisi d\u00fcz, ay\u0131rma derecesi iyi ve ortak piklerin say\u0131s\u0131 b\u00fcy\u00fckt\u00fc. Xihuangcao, Xianwen Xiangchacai, Xianxiang Xiangchacai ve Longye Xiangchacai'nin olu\u015fturulan kontrol haritalar\u0131nda s\u0131ras\u0131yla 19, 24, 26 ve 21 ortak tepe noktas\u0131 kalibre edilmi\u015ftir. Ortak tepe noktalar\u0131ndaki farkl\u0131l\u0131klara dayanarak, d\u00f6rt temel bitki \u00f6nsel olarak ay\u0131rt edilebilir. Toplanan Xianwen Xiangchacai ve Longye Xiangchacai partilerinin say\u0131s\u0131n\u0131n az olmas\u0131 nedeniyle, ara\u015ft\u0131rma s\u00fcrecinde hatalardan ka\u00e7\u0131nmak i\u00e7in, ortak pikler incelenirken kalibrasyon i\u00e7in daha y\u00fcksek yan\u0131t de\u011ferlerine, daha iyi ay\u0131rma derecesine ve pik \u015fekline sahip kromatografik pikler se\u00e7ilmi\u015ftir. Benzerlik de\u011ferlendirme sonu\u00e7lar\u0131, men\u015fe yerindeki farkl\u0131l\u0131klar nedeniyle, ayn\u0131 orijinal t\u0131bbi malzemeler aras\u0131nda belirli farkl\u0131l\u0131klar oldu\u011funu g\u00f6stermektedir; Xihuangcao ile di\u011fer \u00fc\u00e7 temel t\u0131bbi malzeme aras\u0131ndaki benzerli\u011fin nispeten d\u00fc\u015f\u00fck oldu\u011fu ve a\u00e7\u0131k\u00e7a ay\u0131rt edilebildi\u011fi farkl\u0131 temel t\u0131bbi malzemeler aras\u0131nda da farkl\u0131l\u0131klar vard\u0131r; Xianhua kokulu \u00e7ay sebzesi ile Xianwen kokulu \u00e7ay sebzesi aras\u0131ndaki benzerlik nispeten y\u00fcksektir ve Changye kokulu \u00e7ay sebzesi ile Xianhua kokulu \u00e7ay sebzesi aras\u0131ndaki benzerlik de nispeten y\u00fcksektir. Bunun nedeni, Xianhua kokulu \u00e7ay sebzesi, Xianwen kokulu \u00e7ay sebzesi ve Changye kokulu \u00e7ay sebzesinin benzer kimyasal bile\u015fim t\u00fcrlerine sahip buru\u015fuk yaprakl\u0131 kokulu \u00e7ay sebzesi grubuna ait bitkiler olmas\u0131 olabilir. Mevcut ara\u015ft\u0131rmalar, Xihuangcao t\u0131bbi malzemelerinin orijinal bitkilerinde bulunan ve esas olarak terpenler, flavonoidler, fenolik asitler ve sterolleri i\u00e7eren kimyasal bile\u015fen t\u00fcrlerinde \u00e7ok az fark oldu\u011funu g\u00f6stermi\u015ftir. Bunlar aras\u0131nda diterpenler, Xihuangcao \u015fifal\u0131 bitkisinin \u00f6nemli bile\u015fenleridir. Orijinal bitki Xihuangcao'da bulunan ana diterpen t\u00fcr\u00fc enantiyomerik kaempferol t\u00fcr\u00fc iken, do\u011frusal kokulu \u00e7ay sebzesi ve lifli \u00e7i\u00e7ek \u00e7e\u015fidinin yan\u0131 s\u0131ra uzun yaprakl\u0131 kokulu \u00e7ay sebzesi esas olarak re\u00e7ine t\u00fcr\u00fc ve enantiyomerik re\u00e7ine t\u00fcr\u00fc diterpenler i\u00e7erir. Xihuangcao ile do\u011frusal kokulu \u00e7ay sebzesi, lifli kokulu \u00e7ay sebzesi ve uzun yaprakl\u0131 kokulu \u00e7ay sebzesi aras\u0131ndaki d\u00fc\u015f\u00fck benzerli\u011fin diterpen t\u00fcrlerindeki farkl\u0131l\u0131klarla ili\u015fkili olabilece\u011fi, do\u011frusal kokulu \u00e7ay sebzesi, lifli kokulu \u00e7ay sebzesi ve uzun yaprakl\u0131 kokulu \u00e7ay sebzesi aras\u0131ndaki farkl\u0131l\u0131klar\u0131n ise ilgili bile\u015fenlerinin i\u00e7eri\u011fi ve t\u00fcrleriyle ili\u015fkili olabilece\u011fi tahmin edilmektedir.
\nTemel bile\u015fen analizi, Xihuangcao'nun \u00c7izgili Kokulu \u00c7ay Sebzesi, Kokulu \u00c7ay Sebzesi ve Uzun Yaprakl\u0131 Kokulu \u00c7ay Sebzesi'nden her bir temel \u00f6\u011fenin temel bile\u015fen puanlar\u0131ndaki farkla daha da ay\u0131rt edilebilece\u011fini g\u00f6stermi\u015ftir. K\u0131smi en k\u00fc\u00e7\u00fck kareler diskriminant analizi, \u00e7izgi desenli kokulu \u00e7ay bitkisi, lifli kokulu \u00e7ay bitkisi ve uzun yaprakl\u0131 kokulu \u00e7ay bitkisi olmak \u00fczere \u00fc\u00e7 t\u00fcr ilkel bitkiyi \u00f6nemli s\u0131n\u0131fland\u0131rma etkileriyle ay\u0131rt edebilir. Elde edilen 15 s\u0131n\u0131fland\u0131rma belirteci, bu \u00fc\u00e7 farkl\u0131 ilkel bitkiyi ay\u0131rt etmek i\u00e7in b\u00fcy\u00fck \u00f6nem ta\u015f\u0131yan, \u00fc\u00e7\u00fc aras\u0131nda farkl\u0131l\u0131klara neden olan ana karakteristik bile\u015fenlerdir. Bu 15 kromatografik pikin tan\u0131mlanmas\u0131 i\u00e7in daha fazla ara\u015ft\u0131rma ve tan\u0131mlama yap\u0131lmas\u0131 gerekmektedir.
\n\u00d6zetle, bu \u00e7al\u0131\u015fmada olu\u015fturulan Xihuangcao'nun d\u00f6rt bazal bitkisinin UPLC karakteristik haritalar\u0131, karakteristik piklerin kalibrasyonu ve benzerlik de\u011ferlendirmesi yoluyla d\u00f6rt farkl\u0131 bazal bitkiyi \u00f6nceden ay\u0131rt edebilir. D\u00f6rt orijinal bitki t\u00fcr\u00fcn\u00fc daha iyi ay\u0131rt etmek ve diferansiyel bile\u015fenleri ara\u015ft\u0131rmak amac\u0131yla, bu \u00e7al\u0131\u015fmada ayr\u0131ca Xihuangcao'daki d\u00f6rt orijinal bitki t\u00fcr\u00fcn\u00fc tamamen ay\u0131rt etmek i\u00e7in k\u00fcme analizi, temel bile\u015fen analizi ve k\u0131smi en k\u00fc\u00e7\u00fck kareler diskriminant analizi kullan\u0131lm\u0131\u015ft\u0131r. Ara\u015ft\u0131rma sonu\u00e7lar\u0131, Xihuangcao t\u0131bbi malzemelerindeki orijinal bitkilerin tan\u0131mlanmas\u0131, kalite analizi ve de\u011ferlendirilmesi i\u00e7in bilimsel bir y\u00f6ntem ve referans sa\u011flamaktad\u0131r.<\/p>","protected":false},"excerpt":{"rendered":"

Identification of UPLC characteristic spectra of four original plants of Xihuangcao medicinal herbs Xihuang Grass is a unique Chinese herbal medicine in the south of the Five Ridges, which has the effect of clearing heat and removing dampness, cooling blood and removing stasis. It is used for damp heat jaundice, abdominal distention and hypochondriac pain, […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"\nIdentification of UPLC characteristic spectra of four original plants of Xihuangcao medicinal herbs - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/tr\/xihuangcao-medicinal-herbs\/\" \/>\n<meta property=\"og:locale\" content=\"tr_TR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Identification of UPLC characteristic spectra of four original plants of Xihuangcao medicinal herbs - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"Identification of UPLC characteristic spectra of four original plants of Xihuangcao medicinal herbs Xihuang Grass is a unique Chinese herbal medicine in the south of the Five Ridges, which has the effect of clearing heat and removing dampness, cooling blood and removing stasis. 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