{"id":6615,"date":"2024-08-06T12:45:17","date_gmt":"2024-08-06T12:45:17","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=6615"},"modified":"2024-08-06T12:45:17","modified_gmt":"2024-08-06T12:45:17","slug":"using-response-surface-methodology","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/tr\/yanit-yuzeyi-metodolojisi-kullanarak\/","title":{"rendered":"Yan\u0131t Y\u00fczey Metodolojisi Kullan\u0131larak Paraherquamide A Fermantasyon S\u00fcrecinin Optimizasyonu"},"content":{"rendered":"

Yan\u0131t Y\u00fczey Metodolojisi Kullan\u0131larak Paraherquamide A Fermantasyon S\u00fcrecinin Optimizasyonu
\nParazitik nematod hastal\u0131\u011f\u0131, hayvanc\u0131l\u0131\u011f\u0131n geli\u015fimini ciddi \u015fekilde etkileyen \u00e7iftlik hayvanlar\u0131 ve k\u00fcmes hayvanlar\u0131n\u0131n parazitik bir hastal\u0131\u011f\u0131d\u0131r. \u015eu anda, avernektinler d\u00fcnya \u00e7ap\u0131nda veteriner klinik uygulamalar\u0131nda en yayg\u0131n kullan\u0131lan antiparaziter ila\u00e7lard\u0131r. Bununla birlikte, bu ilac\u0131n k\u00f6t\u00fcye kullan\u0131lmas\u0131 nedeniyle, bir\u00e7ok alanda ilaca diren\u00e7li su\u015flar ortaya \u00e7\u0131km\u0131\u015ft\u0131r. 1980'lerin sonlar\u0131nda \u00c7in bu t\u00fcr bir ilac\u0131 tan\u0131tt\u0131 ve yayg\u0131n olarak kulland\u0131 ve \u015fimdi evcil koyun gastrointestinal nematodlar\u0131 avermektin ila\u00e7lar\u0131na kar\u015f\u0131 \u00e7e\u015fitli derecelerde diren\u00e7 geli\u015ftirdi. Startect \u00ae, d\u00fcnyan\u0131n en b\u00fcy\u00fck hayvan sa\u011fl\u0131\u011f\u0131 ila\u00e7 ve a\u015f\u0131 \u015firketi olan Zoetis taraf\u0131ndan geli\u015ftirilen, \u00e7iftlik hayvanlar\u0131 ve k\u00fcmes hayvanlar\u0131n\u0131n paraziter hastal\u0131klar\u0131n\u0131 ve ila\u00e7 direnci sorunlar\u0131n\u0131 etkili bir \u015fekilde \u00f6nleyebilen ve \u00e7\u00f6zebilen yeni bir t\u00fcr koyun oral k\u0131sa etkili deworming ilac\u0131d\u0131r. Bu ilac\u0131n aktif bile\u015fenleri derquantel (10mg\/mL) ve avermektindir (1mg\/mL). Startect\u00ae'in standart dozu 1mL\/5kg'd\u0131r (s\u0131v\u0131 hacmi\/koyun v\u00fccut a\u011f\u0131rl\u0131\u011f\u0131) ve her 50kg yeti\u015fkin koyun bir kez uygulanmak \u00fczere yakla\u015f\u0131k 0.1g Dexmedetomidine gerektirir. Birle\u015fmi\u015f Milletler G\u0131da ve Tar\u0131m \u00d6rg\u00fct\u00fc (FAO) istatistiklerine g\u00f6re d\u00fcnya koyun varl\u0131\u011f\u0131 1,2 milyara ula\u015fm\u0131\u015ft\u0131r. Dolay\u0131s\u0131yla, Dequnt i\u00e7in potansiyel talep y\u00fczlerce ton veya daha da y\u00fcksektir ve pazar talebi \u00e7ok b\u00fcy\u00fckt\u00fcr.<\/p>\n

Dextrant, esas olarak PA'n\u0131n amid karbonil indirgenmesi ve deoksijenasyonu yoluyla elde edilen fungal metabolit Paraherquamide A'n\u0131n (PA) kimyasal bir t\u00fcrevidir (bkz. \u015eekil 1). Bu nedenle, Dequnte'nin end\u00fcstriyel hammadde tedariki esas olarak PA \u00fcretiminden etkilenmektedir. Bununla birlikte, Paraherquamide gibi do\u011fal \u00fcr\u00fcnlerin kimyasal yap\u0131s\u0131 karma\u015f\u0131kt\u0131r. \u0130zopren veya izopren epoksitler gibi end\u00fcstriyel hammaddelerden kimyasal olarak sentezlenebilmelerine ra\u011fmen, kimyasal reaksiyon ad\u0131mlar\u0131 46 ad\u0131ma kadar ula\u015fabilir, bu da y\u00fcksek maliyetlere ve son derece d\u00fc\u015f\u00fck verime (2.5%) neden olarak end\u00fcstriyel \u00f6l\u00e7ekte \u00fcretilmesini zorla\u015ft\u0131r\u0131r. Mikrobiyal fermantasyon yoluyla PA \u00fcretimi \u015fu anda bu bile\u015fi\u011fi b\u00fcy\u00fck \u00f6l\u00e7ekte elde etmenin etkili bir yoludur. PA ilk olarak Penicillium cinsine ait bir mantar olan Penicillium paraherquei t\u00fcr\u00fcn\u00fcn fermantasyon \u00fcr\u00fcn\u00fcnden izole edilmi\u015ftir. Bununla birlikte, vah\u015fi tip Penicillium taraf\u0131ndan \u00fcretilen PA'n\u0131n g\u00fcc\u00fc y\u00fcksek de\u011fildi, bu da daha fazla deneysel ara\u015ft\u0131rma ve end\u00fcstriyel uygulamay\u0131 engelledi. Fungal metabolitlerin uygulama ara\u015ft\u0131rmalar\u0131nda, hedef metabolitin etki seviyesi sadece su\u015fun kendi performans\u0131na de\u011fil, daha da \u00f6nemlisi, \u00fcretim kapasitesini tam olarak kullanmak i\u00e7in fermantasyon ve yeti\u015ftirme ko\u015fullar\u0131n\u0131n optimize edilmesine ba\u011fl\u0131d\u0131r. Yan\u0131t y\u00fczeyi metodolojisi \u015fu anda mikrobiyal fermantasyon k\u00fclt\u00fcr\u00fcn\u00fc optimize etmek i\u00e7in yayg\u0131n olarak kullan\u0131lmaktad\u0131r. Bu y\u00f6ntem deneysel tasar\u0131m ve matematiksel modellemeyi birle\u015ftirir ve daha az deney, daha k\u0131sa d\u00f6ng\u00fcler, regresyon denklemlerinin elde edilmesinde daha y\u00fcksek do\u011fruluk, daha iyi tahmin performans\u0131 ve \u00e7e\u015fitli fakt\u00f6rler aras\u0131ndaki etkile\u015fimin incelenmesi gibi avantajlara sahiptir.<\/p>\n

\u00d6n \u00e7al\u0131\u015fmada, ara\u015ft\u0131rma grubumuz y\u00fcksek PA \u00fcretimine sahip deniz kaynakl\u0131 bir Penicillium mantar\u0131 KWF31 ke\u015ffetti. \u0130lk ke\u015fif, fermantasyon g\u00fcc\u00fcn\u00fcn Chatelier ortam\u0131 alt\u0131nda 55.8 \u00b1 3.7mg\/L oldu\u011funu ve bunun end\u00fcstriyel \u00fcretim gereksinimlerini kar\u015f\u0131lamad\u0131\u011f\u0131n\u0131 g\u00f6stermi\u015ftir. Bu \u00e7al\u0131\u015fman\u0131n amac\u0131, Plackett Burman tasar\u0131m\u0131, en dik t\u0131rmanma tasar\u0131m\u0131 ve Box Behnken tasar\u0131m\u0131 gibi yan\u0131t y\u00fczeyi y\u00f6ntemlerini kullanarak KWF31 su\u015funun fermantasyon ortam\u0131n\u0131 optimize etmek ve y\u00fcksek verimli ve istikrarl\u0131 bir PA \u00fcretim s\u00fcreci teknolojisi \u015femas\u0131 olu\u015fturmakt\u0131r.<\/p>\n

\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"PA, benzersiz bir kimyasal yap\u0131ya ve m\u00fckemmel b\u00f6cek direnci aktivitesine sahip bir izopentenil indol alkaloiddir. Ticari b\u00f6cek diren\u00e7li ajan Dextrant'\u0131n sentezi i\u00e7in bir hammaddedir ve y\u00fcksek pazar de\u011ferine ve b\u00fcy\u00fck pazar talebine sahiptir. Bununla birlikte, vah\u015fi tip Penicillium su\u015fu, birden fazla kimyasal sentez a\u015famas\u0131 ve d\u00fc\u015f\u00fck verim ile d\u00fc\u015f\u00fck PA verimine sahiptir ve bu da pazar talebini kar\u015f\u0131lamay\u0131 zorla\u015ft\u0131rmaktad\u0131r. Bu \u00e7al\u0131\u015fmada, sekiz fakt\u00f6rden \u00e7alkalamal\u0131 \u015fi\u015felerde yabani KWF31 su\u015fu taraf\u0131ndan PA'n\u0131n yatay fermantasyon \u00fcretimini \u00f6nemli \u00f6l\u00e7\u00fcde etkileyen \u00fc\u00e7 ortam bile\u015fenini taramak i\u00e7in Plackett Burman tasar\u0131m\u0131 kullan\u0131lm\u0131\u015ft\u0131r: \u00e7\u00f6z\u00fcn\u00fcr ni\u015fasta, glikoz ve maya \u00f6z\u00fct\u00fc; Konsantrasyonlar\u0131, t\u0131rmanma deneyleri ve Box Behnken tasar\u0131m\u0131 kullan\u0131larak kademeli olarak optimize edilmi\u015ftir. Optimize edilmi\u015f optimum konsantrasyonlar \u00e7\u00f6z\u00fcn\u00fcr ni\u015fasta 31.0g\/L, glikoz 1208g\/L ve maya \u00f6z\u00fct\u00fc 14.5g\/L'dir. Bu ortamda, yabani tip KWF31 su\u015fu, sallama \u015fi\u015fesi yatay fermantasyonu s\u0131ras\u0131nda PA \u00fcretimi i\u00e7in 243,7 mg\/L'lik y\u00fcksek bir potens g\u00f6stermi\u015ftir; bu, optimizasyon \u00f6ncesine g\u00f6re (55,8 mg\/L) 3,4 kat daha y\u00fcksektir. Bu \u00e7al\u0131\u015fmada deniz mantar\u0131 KWF31 taraf\u0131ndan PA fermantasyon \u00fcretimi i\u00e7in olu\u015fturulan s\u00fcre\u00e7 iyi bir tekrarlanabilirlik, stabilite ve kontrol edilebilirli\u011fe sahiptir. Bu temelde, sanayile\u015fmi\u015f fermantasyon s\u00fcre\u00e7lerinin daha da geli\u015ftirilmesi, \u00c7in'de hayvanc\u0131l\u0131\u011f\u0131n sa\u011fl\u0131kl\u0131 geli\u015fimini te\u015fvik etmek i\u00e7in \u00f6nemli teorik ve pratik \u00f6neme sahip olan \u00c7in'de yeni anti nematod pestisitlerin geli\u015ftirilmesinin temelini olu\u015fturmaktad\u0131r.<\/p>","protected":false},"excerpt":{"rendered":"

Optimization of Paraherquamide A Fermentation Process Using Response Surface Methodology Parasitic nematode disease is a parasitic disease of livestock and poultry that seriously affects the development of animal husbandry. At present, avernectins are the most widely used antiparasitic drugs in veterinary clinical practice worldwide. However, due to the abuse of this drug, drug-resistant strains have […]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"\nOptimization of Paraherquamide A Fermentation Process Using Response Surface Methodology - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/tr\/yanit-yuzeyi-metodolojisi-kullanarak\/\" \/>\n<meta property=\"og:locale\" content=\"tr_TR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Optimization of Paraherquamide A Fermentation Process Using Response Surface Methodology - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"Optimization of Paraherquamide A Fermentation Process Using Response Surface Methodology Parasitic nematode disease is a parasitic disease of livestock and poultry that seriously affects the development of animal husbandry. 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