{"id":6545,"date":"2024-08-06T12:29:50","date_gmt":"2024-08-06T12:29:50","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=6545"},"modified":"2024-08-06T12:29:50","modified_gmt":"2024-08-06T12:29:50","slug":"tu-niu-knee","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/tr\/tu-niu-knee\/","title":{"rendered":"Ekstraksiyon S\u00fcrecinin Optimizasyonu ve Tu Niu Dizinden Elde Edilen Etanol Ekstrakt\u0131n\u0131n \u0130n vitro Anti-insan Akci\u011fer Adenokarsinomu A549 H\u00fccre Aktivitesi"},"content":{"rendered":"<p>Ekstraksiyon S\u00fcrecinin Optimizasyonu ve Tu Niu Dizinden Elde Edilen Etanol Ekstrakt\u0131n\u0131n \u0130n vitro Anti-insan Akci\u011fer Adenokarsinomu A549 H\u00fccre Aktivitesi<br \/>\n\u00c7in \u015fifal\u0131 bitkisi olarak da bilinen Achyranthes aspera L. detoksifikasyon, anti-kanser, tarama ve di\u00fcrez etkilerine sahiptir. Miao, Yao, Zhuang, Dai ve Yi etnik gruplar\u0131 da dahil olmak \u00fczere g\u00fcneybat\u0131 \u00c7in'deki bir\u00e7ok etnik az\u0131nl\u0131k taraf\u0131ndan yayg\u0131n olarak kullan\u0131lmaktad\u0131r. Achyranthes bidentata, ana \u00e7ekirdek olarak oleanolik asit i\u00e7eren triterpen saponinler i\u00e7erir. Hidrolizat oleanolik asit antioksidan, anti-inflamatuar, anti ateroskleroz ve di\u011fer biyolojik aktivitelere sahiptir. Kan \u015fekerini d\u00fc\u015f\u00fcrme, antibakteriyel, analjezik ve anti-enflamatuar \u00f6zellikler gibi i\u015flevsel \u00f6zellikleri nedeniyle Achyranthes bidentata t\u0131pta benzersiz bir uygulama de\u011ferine ve geni\u015f geli\u015fme olanaklar\u0131na sahiptir. Bu nedenle, Achyranthes bidentata'n\u0131n aktif bile\u015fenlerinin ve fonksiyonel \u00f6zelliklerinin incelenmesi b\u00fcy\u00fck \u00f6nem ta\u015f\u0131maktad\u0131r.<\/p>\n<p>\u00d6nceki ara\u015ft\u0131rmac\u0131lar Achyranthes bidentata'dan 58 \u00f6nemli madde izole etmi\u015f ve tan\u0131mlam\u0131\u015ft\u0131r; bunlar\u0131n aras\u0131nda izole edilen ve elde edilen ana bile\u015fenler \u00e7o\u011funlukla polisakkaritler, flavonoidler, terpenler, bitki sterolleri, saponinler vb.dir. Anti-enflamatuar, antioksidan, hipoglisemik ve antibakteriyel gibi \u00e7e\u015fitli t\u0131bbi \u00f6zelliklere sahiptirler, bu da t\u0131p alan\u0131nda geni\u015f bir geli\u015fme beklentisine sahip olmalar\u0131n\u0131 sa\u011flar. Achyranthes bidentata'n\u0131n hem su ekstresi hem de alkol ekstresi iyi antibakteriyel aktiviteye sahiptir, ancak in vitro anti-kanser aktivitesi ve klinik etkileri ve mekanizmalar\u0131 hen\u00fcz \u00e7al\u0131\u015f\u0131lmam\u0131\u015ft\u0131r. Bu \u00e7al\u0131\u015fma, Eucommia ulmoides'in etanol ekstrakt\u0131n\u0131 Eucommia ulmoides'ten \u00e7\u0131karmak i\u00e7in etanol refl\u00fc y\u00f6ntemini kullanmay\u0131 ama\u00e7lamaktad\u0131r. Tek bir fakt\u00f6re dayal\u0131 olarak, verimli ve minimum tahribatl\u0131 bir ekstraksiyon s\u00fcreci bulmak i\u00e7in yan\u0131t y\u00fczeyi optimizasyonu ekstraksiyon s\u00fcreci benimsenmi\u015ftir. Eucommia ulmoides etanol ekstrakt\u0131n\u0131n insan akci\u011fer adenokarsinomu A549 h\u00fccreleri \u00fczerindeki etkisi, Eucommia ulmoides'in kullan\u0131m\u0131 ve Eucommia ulmoides etanol ekstrakt\u0131n\u0131n antikanser fonksiyonunun geli\u015ftirilmesi i\u00e7in teorik temel sa\u011flamak amac\u0131yla h\u00fccresel d\u00fczeyde \u00f6nceden incelenmi\u015ftir.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-6546\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-237.png\" alt=\"\" width=\"330\" height=\"237\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-6547\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/2-198.png\" alt=\"\" width=\"315\" height=\"227\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-6548\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/3-186.png\" alt=\"\" width=\"332\" height=\"240\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-6549\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/4-165.png\" alt=\"\" width=\"316\" height=\"236\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-6550\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/5-149.png\" alt=\"\" width=\"331\" height=\"199\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-6551\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/6-135.png\" alt=\"\" width=\"328\" height=\"476\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-6552\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/7-123.png\" alt=\"\" width=\"633\" height=\"396\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-6553\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/8-111.png\" alt=\"\" width=\"607\" height=\"732\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/8-111.png 607w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/8-111-597x720.png 597w\" sizes=\"(max-width: 607px) 100vw, 607px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-6554\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/9-103.png\" alt=\"\" width=\"331\" height=\"255\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-6555\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/10-82.png\" alt=\"\" width=\"335\" height=\"194\" \/><br \/>\nRadix Achyranthes'ten aktif maddeleri \u00e7\u0131karmak i\u00e7in etanol refl\u00fc kullan\u0131lm\u0131\u015f ve Radix Achyranthes'in etanol ekstrakt\u0131n\u0131 \u00e7\u0131karmak i\u00e7in en uygun i\u015flem ko\u015fullar\u0131 tek fakt\u00f6rl\u00fc deneyler ve yan\u0131t y\u00fczeyi optimizasyonu yoluyla belirlenmi\u015ftir. S\u0131cakl\u0131k 85,84 \u2103, s\u0131v\u0131\/madde oran\u0131 41,46:1 ve ekstraksiyon s\u00fcresi 2,14 saat olarak belirlenmi\u015ftir. Tahmini teorik ekstraksiyon oran\u0131 9.36% idi. Ger\u00e7ek \u00e7al\u0131\u015fma ko\u015fullar\u0131 g\u00f6z \u00f6n\u00fcne al\u0131nd\u0131\u011f\u0131nda, 90 \u2103 s\u0131cakl\u0131k, 40:1 s\u0131v\u0131\/madde oran\u0131 ve 2 saatlik ekstraksiyon s\u00fcresi kullan\u0131larak, bu ko\u015fullar alt\u0131nda elde edilen toprak inek dizinin etanol ekstrakt\u0131n\u0131n ekstraksiyon oran\u0131 9.23% olup, tahmin edilen 9.36% de\u011ferinden biraz farkl\u0131d\u0131r. Sitotoksisite deneyinin sonu\u00e7lar\u0131, etanol ekstrakt\u0131n\u0131n konsantrasyonu artt\u0131k\u00e7a, A549 h\u00fccrelerinin hayatta kalma oran\u0131n\u0131n h\u0131zla azald\u0131\u011f\u0131n\u0131 g\u00f6stermi\u015ftir. Etanol ekstrakt\u0131n\u0131n konsantrasyonu 256 \u03bcg\/mL oldu\u011funda, A549 h\u00fccrelerinin inhibisyon oran\u0131 94.65%'ye ula\u015fm\u0131\u015f ve etanol ekstrakt\u0131n\u0131n kanser h\u00fccreleri \u00fczerindeki \u00e7izik iyile\u015fme oran\u0131, bo\u015f kontrol grubununkinden \u00f6nemli \u00f6l\u00e7\u00fcde d\u00fc\u015f\u00fck olmu\u015ftur (P&lt;0.001), bu da inek dizinin etanol ekstrakt\u0131n\u0131n belirli bir anti-kanser aktivitesine sahip oldu\u011funu g\u00f6stermektedir. Daha sonra, gaz kromatografisi-k\u00fctle spektrometresi (GC-MS) ve y\u00fcksek performansl\u0131 s\u0131v\u0131 kromatografisi tandem kuadrupol\/u\u00e7u\u015f zaman\u0131 k\u00fctle spektrometresi (HPLC-Q-TOF\/MS) gibi teknikler, Radix Achyranthes&#039;in etanol ekstresinin kimyasal bile\u015fimini niteliksel ve niceliksel olarak incelemek i\u00e7in kullan\u0131labilir ve tam bir kalite kontrol sistemi kurulmaya \u00e7al\u0131\u015f\u0131labilir. Ayn\u0131 zamanda, Radix Achyranthes ekstresinin \u00e7\u0131plak fare A549 akci\u011fer kanseri h\u00fccre nakli t\u00fcm\u00f6r modeli \u00fczerindeki etkisi, anti-kanser mekanizmas\u0131n\u0131 daha fazla ara\u015ft\u0131rmak i\u00e7in incelenebilir.<\/p>","protected":false},"excerpt":{"rendered":"<p>Optimization of Extraction Process and In vitro Anti human Lung Adenocarcinoma A549 Cell Activity of Ethanol Extract from Tu Niu Knee Achyranthes aspera L., also known as Chinese medicinal herb, has the effects of detoxification, anti-cancer, dredging, and diuresis. It is commonly used by many ethnic minorities in southwestern China, including the Miao, Yao, Zhuang, [&hellip;]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.0 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Optimization of Extraction Process and In vitro Anti human Lung Adenocarcinoma A549 Cell Activity of Ethanol Extract from Tu Niu Knee - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/tr\/tu-niu-knee\/\" \/>\n<meta property=\"og:locale\" content=\"tr_TR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Optimization of Extraction Process and In vitro Anti human Lung Adenocarcinoma A549 Cell Activity of Ethanol Extract from Tu Niu Knee - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"Optimization of Extraction Process and In vitro Anti human Lung Adenocarcinoma A549 Cell Activity of Ethanol Extract from Tu Niu Knee Achyranthes aspera L., also known as Chinese medicinal herb, has the effects of detoxification, anti-cancer, dredging, and diuresis. 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