{"id":5786,"date":"2024-08-04T21:11:05","date_gmt":"2024-08-04T21:11:05","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=5786"},"modified":"2024-08-04T21:11:05","modified_gmt":"2024-08-04T21:11:05","slug":"verbena","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/tr\/verbena\/","title":{"rendered":"UPLC \u00d6zellik Haritas\u0131 ve Bir Test \u00c7oklu De\u011ferlendirme Y\u00f6ntemine Dayal\u0131 Verbena Kalite De\u011ferlendirmesi ve Standart Dekoksiyon Dozaj\u0131n\u0131n Aktar\u0131m Yasas\u0131 \u00dczerine Ara\u015ft\u0131rma"},"content":{"rendered":"

UPLC \u00d6zellik Haritas\u0131 ve Bir Test \u00c7oklu De\u011ferlendirme Y\u00f6ntemine Dayal\u0131 Verbena Kalite De\u011ferlendirmesi ve Standart Dekoksiyon Dozaj\u0131n\u0131n Aktar\u0131m Yasas\u0131 \u00dczerine Ara\u015ft\u0131rma
\nVerbenaceae familyas\u0131ndan Verbena officinalis L. bitkisinin kuru toprak \u00fcst\u00fc k\u0131sm\u0131 olan mine \u00e7i\u00e7e\u011fi ilk olarak \"\u00dcnl\u00fc Hekimler Katalo\u011fu \"nda kaydedilmi\u015f ve alt s\u0131n\u0131f olarak s\u0131n\u0131fland\u0131r\u0131lm\u0131\u015ft\u0131r. Mine \u00e7i\u00e7e\u011finin kimyasal bile\u015fenleri ba\u015fl\u0131ca iridoid glikozitler, flavonoidler, steroller, triterpenler, polifenoller, u\u00e7ucu ya\u011flar, tanenler, beta karoten, kafeik asit, \u015fekerler vb.dir. Ana aktif bile\u015fenler iridoid glikozitlerdir. Serin bir do\u011fas\u0131, ac\u0131 bir tad\u0131 vard\u0131r ve karaci\u011fer ve dalak meridyenlerine aittir. Kan dola\u015f\u0131m\u0131n\u0131 te\u015fvik etme, kan staz\u0131n\u0131 da\u011f\u0131tma, detoksifiye etme, di\u00fcrezi te\u015fvik etme, sar\u0131l\u0131\u011f\u0131 azaltma ve s\u0131tmay\u0131 durdurma etkilerine sahiptir. Klinik olarak, dismenore, amenore, farenjit, apse, \u00f6dem, sar\u0131l\u0131k ve s\u0131tma gibi semptomlar\u0131n birikmesi i\u00e7in kullan\u0131l\u0131r. Mine \u00e7i\u00e7e\u011finin kaynaklar\u0131 bol olup, bunlar aras\u0131nda iridoid glikozitlerin antioksidan, hepatoprotektif, n\u00f6roprotektif, kalp ve beyin iskemisine kar\u015f\u0131 koruyucu, anti-enflamatuar vb. gibi \u00e7e\u015fitli etkileri vard\u0131r. \u00c7in bitkisel dekoksiyonlar\u0131 (standart dekoksiyonlar) klinik uygulamada en yayg\u0131n kullan\u0131lan geleneksel \u00c7in t\u0131bb\u0131 bile\u015fik preparatlar\u0131d\u0131r ve ayn\u0131 zamanda \u00c7in t\u0131bb\u0131 tarihinde en uzun ve en yayg\u0131n kullan\u0131lan preparatlard\u0131r. Geleneksel \u00c7in t\u0131bb\u0131 dekoksiyon par\u00e7alar\u0131, k\u0131r\u0131k duvar dekoksiyon par\u00e7alar\u0131, geleneksel \u00c7in t\u0131bb\u0131 form\u00fcl gran\u00fclleri ve di\u011fer yeni \u00c7in t\u0131bb\u0131 dekoksiyon par\u00e7alar\u0131 olsun, hepsi dekoksiyon veya suyla y\u0131kama yoluyla al\u0131n\u0131r. Bu nedenle, di\u011fer \u00c7in t\u0131bb\u0131 preparatlar\u0131n\u0131n incelenmesi i\u00e7in tercih edilen kontrol olarak standart kaynatma ara\u015ft\u0131rmas\u0131n\u0131n kullan\u0131lmas\u0131 b\u00fcy\u00fck \u00f6nem ta\u015f\u0131maktad\u0131r. Bu \u00e7al\u0131\u015fma, geleneksel \u00c7in t\u0131bb\u0131 mine \u00e7i\u00e7e\u011fi form\u00fclasyonlar\u0131n\u0131n ara\u015ft\u0131r\u0131lmas\u0131 ve kalite de\u011ferlendirmesi i\u00e7in temel olu\u015fturarak, kaynatma par\u00e7alar\u0131ndan standart kaynatmalara kantitatif transfer yasas\u0131n\u0131 a\u00e7\u0131klamaktad\u0131r.
\nBu \u00e7al\u0131\u015fma, verbascoside dilimlerinde verbascoside, 5-hydroxyverbascoside ve verbascoside haz\u0131rlanmas\u0131na odaklanmakta ve UPLC karakteristik haritas\u0131n\u0131 olu\u015fturmaktad\u0131r. Harita bilgileri kimyasal mod analizi kullan\u0131larak analiz edilir ve verbascoside, 5-hydroxyverbascoside ve verbascoside i\u00e7eri\u011fi, bir test \u00e7oklu de\u011ferlendirme y\u00f6ntemi ile birlikte ultra y\u00fcksek performansl\u0131 s\u0131v\u0131 kromatografisi ile ayn\u0131 anda belirlenir. Sonu\u00e7lar harici standart y\u00f6ntemle elde edilenlerle kar\u015f\u0131la\u015ft\u0131r\u0131l\u0131r. Standart kaynatma verimi, verbascoside, 5-hydroxyverbascoside ve verbascoside i\u00e7eri\u011fi \u00f6l\u00e7\u00fcl\u00fcr, transfer oran\u0131 hesaplan\u0131r ve buna dayanarak, \u00e7e\u015fitli kalite g\u00f6stergelerinin rasyonelli\u011fini g\u00f6stermek i\u00e7in verbascoside kaynatma par\u00e7alar\u0131n\u0131n standart kaynatmaya transferi \u00fczerine bir \u00e7al\u0131\u015fma y\u00fcr\u00fct\u00fcl\u00fcr.<\/p>\n

\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"
\nMine \u00e7i\u00e7e\u011fi numunesinin karakteristik spektrumunun izokratik el\u00fcsyonu bazal ayr\u0131m\u0131 sa\u011flayamaz, bu nedenle gradyan el\u00fcsyon kullan\u0131lm\u0131\u015ft\u0131r. \u00d6n deneylerde, bu \u00e7al\u0131\u015fmada metanol su, asetonitril su, asetonitril-0.1% fosforik asit \u00e7\u00f6zeltisi ve asetonitril-0.05% fosforik asit \u00e7\u00f6zeltisi gibi mobil fazlar kar\u015f\u0131la\u015ft\u0131r\u0131lm\u0131\u015ft\u0131r. Son olarak, mobil faz olarak asetonitril-0.05% fosforik asit \u00e7\u00f6zeltisi se\u00e7ilmi\u015ftir. Yukar\u0131daki kromatografik ko\u015fullar alt\u0131nda, kromatografik pik ayr\u0131m\u0131, teorik plaka say\u0131s\u0131 ve pik \u015fekli parametrelerinin t\u00fcm\u00fc iyiydi. Bu \u00e7al\u0131\u015fmada ekstraksiyon \u00e7\u00f6z\u00fcc\u00fcs\u00fc, ekstraksiyon y\u00f6ntemi ve s\u00fcresi ile kat\u0131-s\u0131v\u0131 oran\u0131n\u0131n ekstraksiyon verimlili\u011fi \u00fczerindeki etkilerini ara\u015ft\u0131rmak i\u00e7in tek fakt\u00f6rl\u00fc bir deneysel sistem kullan\u0131lm\u0131\u015ft\u0131r. Sonu\u00e7lar, ekstraksiyon \u00e7\u00f6z\u00fcc\u00fcs\u00fc 80% metanol, kat\u0131-s\u0131v\u0131 oran\u0131 1:50 (g\/mL) oldu\u011funda ve refl\u00fc ekstraksiyonu 120 dakika boyunca ger\u00e7ekle\u015ftirildi\u011finde ekstraksiyon verimlili\u011finin en y\u00fcksek oldu\u011funu g\u00f6stermi\u015ftir. Bu nedenle, mine \u00e7i\u00e7e\u011fi dilimleri i\u00e7in ekstraksiyon y\u00f6ntemi olarak yukar\u0131daki y\u00f6ntem kullan\u0131lm\u0131\u015ft\u0131r.
\nGeleneksel \u00c7in t\u0131bb\u0131nda \u00e7oklu indeks bile\u015fenlerinin genel kalite kontrol\u00fc, geleneksel \u00c7in t\u0131bb\u0131 kalite kontrol\u00fcn\u00fcn geli\u015ftirilmesinde ka\u00e7\u0131n\u0131lmaz bir e\u011filim haline gelmi\u015ftir. Karakteristik\/parmak izi spektrumu etkili bir kalite de\u011ferlendirme y\u00f6ntemidir ve tek test \u00e7oklu de\u011ferlendirme analiz y\u00f6ntemiyle birle\u015ftirilmesi kalite de\u011ferlendirmesinin b\u00fct\u00fcnl\u00fc\u011f\u00fcn\u00fc ve do\u011frulu\u011funu sa\u011flayabilir. Bu \u00e7al\u0131\u015fmada, verbascoside kaynatma par\u00e7alar\u0131n\u0131n UPLC karakteristik haritas\u0131 olu\u015fturulmu\u015f, 5 ortak pik belirlenmi\u015f ve 3 bile\u015fen tan\u0131mlanm\u0131\u015ft\u0131r. Analiz s\u00fcresi HPLC'ye k\u0131yasla \u00f6nemli \u00f6l\u00e7\u00fcde k\u0131salm\u0131\u015f ve kromatografik ko\u015fullar 5-hidroksiverbascoside, verbascoside ve verbascoside i\u00e7eri\u011finin belirlenmesi i\u00e7in kullan\u0131labilmi\u015ftir. Temel bile\u015fen analizi, karakteristik piklerin pik alan\u0131 \u00fczerinde ger\u00e7ekle\u015ftirilmi\u015f ve temel bile\u015fenlerin kapsaml\u0131 de\u011ferlendirme modeli, mine \u00e7i\u00e7e\u011finin kalite de\u011ferlendirme ve analiz y\u00f6ntemini daha da geli\u015ftirmi\u015ftir.<\/p>\n

Mine \u00e7i\u00e7e\u011finin 2020 \u00c7in Farmakopesi'ndeki i\u00e7erik belirleme endeksi, her ikisi de d\u00fc\u015f\u00fck polariteye ve d\u00fc\u015f\u00fck suda \u00e7\u00f6z\u00fcn\u00fcrl\u00fc\u011fe sahip triterpenoidler olan toplam oleanolik asit ve ursolik asit miktar\u0131d\u0131r. \u00d6n deneysel ara\u015ft\u0131rma sonu\u00e7lar\u0131, standart mine \u00e7i\u00e7e\u011fi kayna\u011f\u0131ndaki toplam oleanolik asit ve ursolik asit i\u00e7eri\u011finin 2.0 mg\/g'dan az oldu\u011funu ve transfer oran\u0131n\u0131n 6%'den az oldu\u011funu g\u00f6stermektedir. Bu nedenle, \u00f6zellik haritas\u0131na dayal\u0131 olarak, 5-hidroksi verbascoside, verbascoside ve verbascoside i\u00e7eri\u011finin belirlenmesi i\u00e7in \u00e7oklu bir de\u011ferlendirme y\u00f6ntemi olu\u015fturulmu\u015ftur. Mine \u00e7i\u00e7e\u011finin polar bile\u015fenleri esas olarak sikloheksen eter terpenlerdir; bunlar aras\u0131nda verbascoside ve 5-hydroxyverbascoside en y\u00fcksek i\u00e7eri\u011fe sahip ana bile\u015fenlerdir ve standart kaynatma daha y\u00fcksek bir aktar\u0131m oran\u0131na sahiptir. Baz\u0131 partilerin transfer oran\u0131 100%'yi a\u015farken, 5-hidroksi verbascoside ve verbascoside'in toplam transfer aral\u0131\u011f\u0131 56.6% ile 91.2% aras\u0131ndad\u0131r. Bunun olas\u0131 nedeni, benzer bile\u015fenlerin \u0131s\u0131tma, kaynatma ve di\u011fer i\u015flemler s\u0131ras\u0131nda yap\u0131sal d\u00f6n\u00fc\u015f\u00fcme u\u011framas\u0131d\u0131r. Verbascoside, 5-hydroxyverbascoside ve verbascoside i\u00e7eri\u011fi ve aktar\u0131m oran\u0131 a\u00e7\u0131s\u0131ndan 18 parti standart verbascoside kaynatma aras\u0131nda farkl\u0131l\u0131klar vard\u0131r ve bu farkl\u0131l\u0131klar kaynatma par\u00e7alar\u0131n\u0131n i\u00e7eri\u011fi ve dokusuyla ilgili olabilir. Baz\u0131 kaynatma par\u00e7alar\u0131 daha a\u00e7\u0131k renklere, daha uzun depolama s\u00fcrelerine ve i\u015fleme s\u0131ras\u0131nda daha fazla d\u00f6k\u00fcnt\u00fcye sahiptir, bu da i\u00e7erik ve aktar\u0131m h\u0131z\u0131ndaki b\u00fcy\u00fck dalgalanmalar\u0131n nedeni olabilir. Se\u00e7ilen \u00fc\u00e7 bile\u015fen mine \u00e7i\u00e7e\u011finde y\u00fcksek bir i\u00e7eri\u011fe sahiptir ve antioksidan, hepatoprotektif, n\u00f6roprotektif, kalp ve beyin iskemisini koruyucu, anti-enflamatuar ve di\u011fer y\u00f6nlerden iyi farmakolojik aktivitelere sahiptir. Bunlar, mine \u00e7i\u00e7e\u011finin farmakolojik etkilerini g\u00f6stermesi i\u00e7in ana malzeme temellerinden biridir.<\/p>\n

Tek test \u00e7oklu de\u011ferlendirme y\u00f6nteminin sonu\u00e7lar\u0131, harici standart y\u00f6nteme k\u0131yasla \u00f6nemli bir fark g\u00f6stermemi\u015ftir ve mine \u00e7i\u00e7e\u011findeki \u00e7oklu bile\u015fenlerin kantitatif analizi i\u00e7in kullan\u0131labilir. \u00c7oklu indeks bile\u015feni tek \u00f6l\u00e7\u00fcm \u00e7oklu de\u011ferlendirme y\u00f6ntemi ile birle\u015ftirilmi\u015f UPLC \u00f6zellik haritas\u0131, mine \u00e7i\u00e7e\u011fi ve mine \u00e7i\u00e7e\u011fi \u00c7in bitkisel preparatlar\u0131n\u0131n \u00fcretim s\u00fcrecinin ara\u015ft\u0131r\u0131lmas\u0131 ve kalite de\u011ferlendirmesi i\u00e7in referans sa\u011flayabilir.<\/p>","protected":false},"excerpt":{"rendered":"

Research on the Quality Evaluation of Verbena and the Transfer Law of Standard Decoction Dosage Based on UPLC Feature Map and One Test Multiple Evaluation Method Verbena officinalis L., a dry aboveground part of the Verbena officinalis L. plant in the Verbenaceae family, was first recorded in the “Catalogue of Famous Physicians” and classified as […]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"\nResearch on the Quality Evaluation of Verbena and the Transfer Law of Standard Decoction Dosage Based on UPLC Feature Map and One Test Multiple Evaluation Method - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/tr\/verbena\/\" \/>\n<meta property=\"og:locale\" content=\"tr_TR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Research on the Quality Evaluation of Verbena and the Transfer Law of Standard Decoction Dosage Based on UPLC Feature Map and One Test Multiple Evaluation Method - 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