{"id":5686,"date":"2024-08-04T20:41:54","date_gmt":"2024-08-04T20:41:54","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=5686"},"modified":"2024-08-04T20:41:54","modified_gmt":"2024-08-04T20:41:54","slug":"dosage-values-of-bu-zha-ye","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/tr\/dosage-values-of-bu-zha-ye\/","title":{"rendered":"UPLC parmak izi spektrumu ve \u00e7ok bile\u015fenli miktar tayinine dayal\u0131 olarak Bu Zha Ye kaynatma par\u00e7alar\u0131n\u0131n ve standart kaynatmalar\u0131n dozaj de\u011ferlerinin aktar\u0131lmas\u0131 \u00fczerine \u00e7al\u0131\u015fma"},"content":{"rendered":"<p>UPLC parmak izi spektrumu ve \u00e7ok bile\u015fenli miktar tayinine dayal\u0131 olarak Bu Zha Ye kaynatma par\u00e7alar\u0131n\u0131n ve standart kaynatmalar\u0131n dozaj de\u011ferlerinin aktar\u0131lmas\u0131 \u00fczerine \u00e7al\u0131\u015fma<br \/>\nBu Zha Ye, Lingnan'da karakteristik bir \u015fifal\u0131 bitkidir ve esas olarak flavonoidler, alkaloidler, terpenler, organik asitler vb. i\u00e7erir. Gastrointestinal peristaltizmi ve mide suyu salg\u0131s\u0131n\u0131 te\u015fvik etme, kan lipitlerini d\u00fc\u015f\u00fcrme, sararmay\u0131 azaltma, anti-enflamatuar, analjezik, kardiyovask\u00fcler koruma, ya\u015flanmay\u0131 geciktirme vb. etkilere sahiptir. Hem ila\u00e7 hem de g\u0131da i\u00e7in \u00e7ift ama\u00e7l\u0131 bir \u00c7in ilac\u0131d\u0131r. Son y\u0131llarda Bu Zha Ye'ye olan ilgi artm\u0131\u015f ve \u00fczerinde yap\u0131lan ara\u015ft\u0131rmalar da giderek daha kapsaml\u0131 hale gelmi\u015ftir.<br \/>\nGeleneksel \u00c7in t\u0131bb\u0131, \u00c7in t\u0131bb\u0131n\u0131n etkinli\u011finin maddi temeli olan ana ila\u00e7 \u015fekli olarak \u00e7o\u011funlukla kaynatma kullan\u0131r. Binlerce y\u0131ll\u0131k klinik uygulamadan sonra, \u00c7in t\u0131bb\u0131n\u0131n standart dekoksiyonlar\u0131 i\u00e7in kalite standartlar\u0131n\u0131n olu\u015fturulmas\u0131, form\u00fcl gran\u00fclleri gibi modern \u00c7in t\u0131bb\u0131 preparatlar\u0131 i\u00e7in kalite standartlar\u0131n\u0131n olu\u015fturulmas\u0131 i\u00e7in \u00f6nemli referanslar sa\u011flayabilir. \u015eu anda, Bu Zha Ye'nin standart kayna\u011f\u0131na ili\u015fkin herhangi bir ara\u015ft\u0131rma raporu bulunmamaktad\u0131r. \u015eirketimizin Bu Zha Ye form\u00fcl gran\u00fcllerinin ulusal standard\u0131 \u00fczerine yapt\u0131\u011f\u0131 ara\u015ft\u0131rmada, Bu Zha Ye form\u00fcl gran\u00fclleri ve ilgili m\u00fcstahzarlar i\u00e7in kalite standartlar\u0131n\u0131n geli\u015ftirilmesi i\u00e7in referans sa\u011flamak \u00fczere Bu Zha Ye kaynatma par\u00e7alar\u0131ndan standart kaynatmaya miktar de\u011ferinin aktar\u0131m yasas\u0131n\u0131 ara\u015ft\u0131rmak i\u00e7in macun verimi, ekstrakt, g\u00f6sterge bile\u015fenlerinin i\u00e7eri\u011fi ve aktar\u0131m oran\u0131, parmak izi spektrumu dahil olmak \u00fczere \"Geleneksel \u00c7in T\u0131bb\u0131 Form\u00fcl Gran\u00fcllerinin Kalite Kontrol\u00fc ve Standart Geli\u015ftirilmesi i\u00e7in Teknik Gereklilikler\" (bundan b\u00f6yle teknik gereklilikler olarak an\u0131lacakt\u0131r) uyar\u0131nca standart kaynatman\u0131n temel kalite g\u00f6stergelerini inceledik.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-5687\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-168.png\" alt=\"\" width=\"781\" height=\"499\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-168.png 781w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-168-768x491.png 768w\" sizes=\"(max-width: 781px) 100vw, 781px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-5688\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/2-154.png\" alt=\"\" width=\"764\" height=\"639\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-5689\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/3-10.jpg\" alt=\"\" width=\"552\" height=\"374\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-5690\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/4-11.jpg\" alt=\"\" width=\"552\" height=\"379\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-5691\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/5-116.png\" alt=\"\" width=\"632\" height=\"366\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-5692\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/6-106.png\" alt=\"\" width=\"639\" height=\"386\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-5693\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/7-98.png\" alt=\"\" width=\"623\" height=\"552\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-5694\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/8-90.png\" alt=\"\" width=\"782\" height=\"531\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/8-90.png 782w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/8-90-768x521.png 768w\" sizes=\"(max-width: 782px) 100vw, 782px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-5695\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/9-84.png\" alt=\"\" width=\"633\" height=\"265\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-5696\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/10-66.png\" alt=\"\" width=\"633\" height=\"704\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-5697\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/11-2.jpg\" alt=\"\" width=\"635\" height=\"145\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-5698\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/12-3.jpg\" alt=\"\" width=\"635\" height=\"74\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-5699\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/13-44.png\" alt=\"\" width=\"635\" height=\"167\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-5700\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/14-35.png\" alt=\"\" width=\"634\" height=\"673\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-5701\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/15-26.png\" alt=\"\" width=\"635\" height=\"523\" \/>Bu Zha Ye'nin kalitesi nispeten hafiftir, ancak kimyasal bile\u015fimi karma\u015f\u0131kt\u0131r. Kaynatma i\u015flemi s\u0131ras\u0131nda, b\u00fcy\u00fck miktarda bile\u015fen ta\u015farak standart kaynatmada daha fazla safs\u0131zl\u0131\u011fa neden olur ve bu da kromatografik ay\u0131rmay\u0131 ve etkili bile\u015fenlerin analizini etkiler. Standart kaynatmay\u0131 i\u015flemek i\u00e7in metanol ve etanol gibi y\u00fcksek polariteye sahip \u00e7\u00f6z\u00fcc\u00fcler kullan\u0131ld\u0131\u011f\u0131nda, parmak izi spektrumu taban \u00e7izgisi karars\u0131zd\u0131r, kromatografik pik ayr\u0131m\u0131 zay\u0131ft\u0131r, kromatografik pikler d\u00fczensizdir ve bir\u00e7ok k\u00fc\u00e7\u00fck pik vard\u0131r. Bu nedenle, standart kaynatma test numunelerinin \u00f6n ar\u0131tma y\u00f6nteminde, safs\u0131zl\u0131klar\u0131n giri\u015fimini azaltmak i\u00e7in etil asetat ekstraksiyonunun ay\u0131rma, safla\u015ft\u0131rma ve zenginle\u015ftirme y\u00f6ntemi ara\u015ft\u0131r\u0131lm\u0131\u015f ve geleneksel ar\u0131tma y\u00f6ntemleriyle kar\u015f\u0131la\u015ft\u0131r\u0131lm\u0131\u015ft\u0131r. Sonu\u00e7lar, ekstraksiyon \u00e7\u00f6z\u00fcc\u00fcs\u00fc olarak etil asetat kullan\u0131lmas\u0131n\u0131n safs\u0131zl\u0131k piklerinin etkisini \u00f6nemli \u00f6l\u00e7\u00fcde iyile\u015ftirebilece\u011fini, ana kromatografik piklerin safl\u0131\u011f\u0131n\u0131 ve ayr\u0131lmas\u0131n\u0131 sa\u011flayabilece\u011fini ve karars\u0131z kromatografik taban \u00e7izgisi ve baz\u0131 kromatografik piklerin k\u00fc\u00e7\u00fck emilimi sorunlar\u0131n\u0131 \u00e7\u00f6zebilece\u011fini g\u00f6stermi\u015ftir. Kromatografik ko\u015fullar\u0131n ara\u015ft\u0131r\u0131lmas\u0131nda, asetonitril-0.1% fosforik asit \u00e7\u00f6zeltisi, asetonitril-0.1% asetik asit \u00e7\u00f6zeltisi, metanol-0.1% formik asit \u00e7\u00f6zeltisi, metanol-0.1% asetik asit \u00e7\u00f6zeltisi gibi farkl\u0131 mobil faz el\u00fcsyon sistemleri incelenmi\u015ftir. Sonu\u00e7lar, el\u00fcsyon sistemi olarak metanol-0.1% asetik asit \u00e7\u00f6zeltisinin kullan\u0131lmas\u0131n\u0131n daha fazla say\u0131da kromatografik pik ve daha iyi genel ay\u0131rma ile sonu\u00e7land\u0131\u011f\u0131n\u0131 g\u00f6stermi\u015ftir. Ultra y\u00fcksek performansl\u0131 s\u0131v\u0131 kromatografisi parmak izi spektrumlar\u0131n\u0131n olu\u015fturulmas\u0131 g\u00f6z \u00f6n\u00fcne al\u0131nd\u0131\u011f\u0131nda, kromatografik kolonlar\u0131n se\u00e7imi \u00e7ok \u00f6nemlidir. Bu nedenle, bu deneyde farkl\u0131 partik\u00fcl boyutlar\u0131na ve markalara sahip kromatografik kolonlar incelenmi\u015f ve sonu\u00e7ta her bir payla\u015f\u0131lan parmak izi pikinin en iyi pik \u015fekli ve \u00e7\u00f6z\u00fcn\u00fcrl\u00fc\u011f\u00fcne sahip Agilent ZORBAX SB C18 kromatografik kolon (2,1 mm \u00d7 150 mm, 1,8 \u03bc m) se\u00e7ilmi\u015ftir.<br \/>\nAra\u015ft\u0131rmalar Bu Zha yapraklar\u0131n\u0131n \u00e7ok say\u0131da flavonoid i\u00e7erdi\u011fini g\u00f6stermi\u015ftir. Xiao ve arkada\u015flar\u0131 UPLC-Q-TOF-MS\/MS teknolojisini kullanarak Bu Zha Ye'den 28 flavonoid ve 3 fenolik asit dahil olmak \u00fczere 31 bile\u015fi\u011fi ba\u015far\u0131yla tan\u0131mlam\u0131\u015ft\u0131r. Farmakolojik \u00e7al\u0131\u015fmalar, flavonoidlerin Bu Zha Ye'nin sindirim, haz\u0131m, \u0131s\u0131 giderme ve rutubet giderici etkileri i\u00e7in \u00f6nemli bir malzeme temeli oldu\u011funu g\u00f6stermi\u015ftir. Vitexin, isovitexin ve narcissin gibi temsili bile\u015fenler Bu Zha Ye'de y\u00fcksek i\u00e7eri\u011fe ve suda belirli \u00e7\u00f6z\u00fcn\u00fcrl\u00fc\u011fe sahiptir. Bu nedenle, Bu Zha Ye standart kaynatma, i\u00e7erik belirleme g\u00f6stergeleri olarak vitexin, isovitexin ve narcissin kullan\u0131r ve standart kaynatma de\u011ferlerinin aktar\u0131lmas\u0131 \u00e7al\u0131\u015fmas\u0131na rehberlik etmek amac\u0131yla kaynatmaya dayal\u0131 Bu Zha Ye kaynatma par\u00e7alar\u0131 i\u00e7in bir i\u00e7erik belirleme y\u00f6ntemi olu\u015fturur.<br \/>\nBu Zha Ye \u015fifal\u0131 bitkileri \u00e7o\u011funlukla Guangdong, Guangxi ve Hainan'da da\u011f\u0131t\u0131lmaktad\u0131r ve Guangdong'da \u00fcretilen otantik \u015fifal\u0131 bitkilere aittir. Yukar\u0131da bahsedilen \u00fc\u00e7 \u00fcretim alan\u0131ndan 15 parti Bu Zha Ye \u015fifal\u0131 otu toplanm\u0131\u015ft\u0131r ve bu da yeterli temsil kabiliyetine sahip oldu\u011funu g\u00f6stermektedir. \u00dc\u00e7 flavonoid i\u00e7in 15 parti standart kaynatma ekstresi, ekstrakt ve 15 parti Bu Zha Ye kaynatma par\u00e7as\u0131n\u0131n belirleme sonu\u00e7lar\u0131ndan, farkl\u0131 partilerden \u00f6rnekler aras\u0131nda belirli farkl\u0131l\u0131klar oldu\u011fu, maksimum ve minimum de\u011ferlerin yakla\u015f\u0131k iki kat farkl\u0131la\u015ft\u0131\u011f\u0131 g\u00f6r\u00fclebilir. Bu farkl\u0131l\u0131k t\u0131bbi malzemelerin kalitesiyle ilgilidir ve Teknik Gerekliliklerin gerekliliklerini kar\u015f\u0131lamaktad\u0131r. Belirgin bir anormal de\u011fer yoktur.<br \/>\nAktar\u0131m oran\u0131 belirleme sonu\u00e7lar\u0131na g\u00f6re, \u00fc\u00e7 flavonoidin ortalama aktar\u0131m oran\u0131 40% ile 60% aras\u0131nda de\u011fi\u015firken, en y\u00fcksek aktar\u0131m oran\u0131na vitexin sahip olmu\u015f, onu narcissin ve isovitexin izlemi\u015f ve genel aktar\u0131m oran\u0131 nispeten sabit kalm\u0131\u015ft\u0131r. Parmak izi spektrumu ara\u015ft\u0131rma sonu\u00e7lar\u0131, Bu Zha Ye kaynatma par\u00e7alar\u0131ndan standart kaynatmaya kadar piklerin oran\u0131nda belirli bir de\u011fi\u015fiklik oldu\u011funu g\u00f6stermi\u015ftir. OPLS-DA analizi, pik 1, 2, 3, 4 ve 6 olmak \u00fczere \u00f6nemli \u00f6l\u00e7\u00fcde farkl\u0131 be\u015f kromatografik pik tespit etmi\u015ftir. Tan\u0131mlanamayan pik 3 hari\u00e7, di\u011fer t\u00fcm kromatografik pikler biliniyordu. Bunlar aras\u0131nda pik 1, 2 ve 4 fenolik asit bile\u015fenleriydi. Bu nedenle, pik 3'\u00fcn bir fenolik asit oldu\u011fu tahmin edilmektedir. Fenolik asit bile\u015fenleri, di\u011fer bile\u015fiklerin sentezi i\u00e7in \u00f6nc\u00fc olarak hizmet edebilen ve ayr\u0131ca \u00e7e\u015fitli bile\u015fiklerden hidrolize edilebilen bitkilerde \u00f6zel bir varl\u0131kt\u0131r. Bu nedenle, Bu Zha Ye'nin standart kaynat\u0131lmas\u0131nda fenolik asit bile\u015fenlerinin ba\u011f\u0131l pik alan\u0131ndaki \u00f6nemli art\u0131\u015f\u0131n, kaynatma i\u015flemi s\u0131ras\u0131nda di\u011fer bile\u015fenlerin hidrolizinden kaynakland\u0131\u011f\u0131 d\u00fc\u015f\u00fcn\u00fclmektedir. Pik 6 isovitexin'in yap\u0131s\u0131 pik S isovitexin'inkine benzer, polarite a\u00e7\u0131s\u0131ndan \u00e7ok az fark vard\u0131r, ancak transfer oran\u0131nda \u00f6nemli bir fark vard\u0131r. Standart kaynatma i\u015flemi s\u0131ras\u0131nda izoviteksin ve izoviteksin aras\u0131nda belirli bir derecede ara d\u00f6n\u00fc\u015f\u00fcm oldu\u011fu ve daha fazla ara\u015ft\u0131rmaya ihtiya\u00e7 oldu\u011fu d\u00fc\u015f\u00fcn\u00fclmektedir.<br \/>\nBu \u00e7al\u0131\u015fma, Bu Zha Ye'nin standart kayna\u011f\u0131n\u0131n temel kalite g\u00f6stergelerine dayanmakta ve Bu Zha Ye kayna\u011f\u0131n\u0131n standart kayna\u011f\u0131n\u0131n de\u011fer aktar\u0131m\u0131 \u00fczerine derinlemesine ara\u015ft\u0131rma ve analizler yaparak Bu Zha Ye form\u00fcl gran\u00fclleri ve ilgili preparatlar i\u00e7in kalite standartlar\u0131n\u0131n form\u00fcle edilmesi i\u00e7in \u00f6nemli referanslar sa\u011flamaktad\u0131r.<\/p>","protected":false},"excerpt":{"rendered":"<p>UPLC parmak izi spektrumu ve \u00e7ok bile\u015fenli miktar tayinine dayal\u0131 olarak Bu Zha Ye kaynatma par\u00e7alar\u0131n\u0131n ve standart kaynatmalar\u0131n dozaj de\u011ferlerinin aktar\u0131m\u0131 \u00fczerine \u00e7al\u0131\u015fma Bu Zha Ye, Lingnan'da esas olarak flavonoidler, alkaloidler, terpenler, organik asitler vb. i\u00e7eren karakteristik bir \u015fifal\u0131 bitkidir. Gastrointestinal peristalsis ve mide suyu salg\u0131lanmas\u0131n\u0131 te\u015fvik edici etkilere sahiptir, [...]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.0 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Study on the transfer of dosage values of Bu Zha Ye decoction pieces and standard decoctions based on UPLC fingerprint spectrum and multi-component quantification - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/tr\/dosage-values-of-bu-zha-ye\/\" \/>\n<meta property=\"og:locale\" content=\"tr_TR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Study on the transfer of dosage values of Bu Zha Ye decoction pieces and standard decoctions based on UPLC fingerprint spectrum and multi-component quantification - 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