{"id":5512,"date":"2024-08-04T19:31:38","date_gmt":"2024-08-04T19:31:38","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=5512"},"modified":"2024-08-04T19:31:38","modified_gmt":"2024-08-04T19:31:38","slug":"uplc-q-orbitrap-hrms","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/tr\/uplc-q-orbitrap-hrms\/","title":{"rendered":"Jinlongdeng'in antibakteriyel aktivitesinin UPLC-Q-Orbitrap HRMS parmak izi ve spektrum etki ili\u015fkisi"},"content":{"rendered":"

Jinlongdeng'in antibakteriyel aktivitesinin UPLC-Q-Orbitrap HRMS parmak izi ve spektrum etki ili\u015fkisi
\nJin Deng Deng, Suanjiang veya Red Maiden olarak da bilinir, Solanaceae familyas\u0131ndan bir bitki olan Physalis alkekengi L. var. franchetii (Mast.) Makino'nun kurutulmu\u015f veya meyve veren kaliksidir. Tad\u0131 ek\u015fi ve ac\u0131, do\u011fas\u0131 so\u011fuk, akci\u011fer ve dalak meridyenlerine aittir. Is\u0131y\u0131 temizleme ve detoksifiye etme, bo\u011faz\u0131 destekleme ve balgam\u0131 \u00e7\u00f6zme, di\u00fcrez ve lenfatik sistemi temizleme etkilerine sahiptir. Klinik olarak bo\u011faz a\u011fr\u0131s\u0131, akci\u011fer \u0131s\u0131s\u0131 \u00f6ks\u00fcr\u00fc\u011f\u00fc ve idrar ka\u00e7\u0131rma tedavisinde kullan\u0131l\u0131r. Jinlongdeng'in kimyasal bile\u015fimi, esas olarak ac\u0131lar, flavonoidler, s\u00fckroz esterleri, fenilpropanoidler, alkaloidler gibi bile\u015fikleri i\u00e7eren \u00e7e\u015fitlidir. Modern farmakolojik \u00e7al\u0131\u015fmalar, Jinlongdeng'in alkol ekstrakt\u0131n\u0131n ve diklorometan ekstrakt\u0131n\u0131n Gram pozitif bakterilere, Gram negatif bakterilere ve Candida albicans'a kar\u015f\u0131 iyi inhibit\u00f6r aktiviteye sahip oldu\u011funu g\u00f6stermi\u015ftir. Ek\u015fi ac\u0131lar\u0131n baz\u0131 bile\u015fenlerinin de \u00f6nemli antibakteriyel etkilere sahip oldu\u011fu kan\u0131tlanm\u0131\u015ft\u0131r. \u015eu anda, Jinlongdeng'in antibakteriyel aktivitesi \u00fczerine hem yurt i\u00e7inde hem de yurt d\u0131\u015f\u0131nda bir\u00e7ok \u00e7al\u0131\u015fma bulunmaktad\u0131r, ancak bunun maddi temeli hen\u00fcz \u00e7ok net de\u011fildir.<\/p>\n

Geleneksel \u00c7in t\u0131bb\u0131n\u0131n kendisi karma\u015f\u0131k ve \u00e7e\u015fitli bile\u015fenlere sahiptir ve terap\u00f6tik etkilerini uygularken \u00e7oklu hedef ve genel d\u00fczenleme \u00f6zelliklerine sahiptir. Geleneksel \u00c7in t\u0131bb\u0131 spektrumu ve etkinli\u011fi aras\u0131ndaki ili\u015fki, geleneksel \u00c7in t\u0131bb\u0131 parmak izi spektrumunun incelenmesine, parmak izi spektrumunun farmakolojik etkilerle ili\u015fkilendirilmesine, spektrum etkinli\u011fi ili\u015fkisi \u00fczerine ara\u015ft\u0131rma yap\u0131lmas\u0131na ve farmakolojik etkilerin uygulanmas\u0131na y\u00f6nelik maddi temelin daha da netle\u015ftirilmesine dayanmaktad\u0131r. \u015eu anda, Jinlongdeng'in parmak izi spektrumu ve spektral etkinlik ili\u015fkisi \u00fczerine nispeten az ara\u015ft\u0131rma bulunmaktad\u0131r. Bu nedenle, bu \u00e7al\u0131\u015fma Jinlongdeng'in spektrum etki ili\u015fkisinden antibakteriyel aktivitesinin maddi temelini ke\u015ffetmeyi ama\u00e7lamaktad\u0131r. \u00dc\u00e7 farkl\u0131 kaynaktan gelen 16 parti Jin Deng fenerinin k\u00fctle spektrometresi parmak izi spektrumlar\u0131n\u0131 olu\u015fturarak ve Staphylococcus aureus ve Escherichia coli'ye kar\u015f\u0131 antibakteriyel aktivitelerini belirleyerek, antibakteriyel aktivitelerinin maddi temelini a\u00e7\u0131kl\u0131\u011fa kavu\u015fturmak ve Jin Deng fenerlerinin daha fazla geli\u015ftirilmesi, kullan\u0131m\u0131 ve kalite kontrol ara\u015ft\u0131rmas\u0131 i\u00e7in bir temel sa\u011flamak amac\u0131yla karakteristik pikler ile antibakteriyel aktiviteleri aras\u0131ndaki korelasyonu ke\u015ffetmek i\u00e7in gri korelasyon analizi kullan\u0131lm\u0131\u015ft\u0131r.<\/p>\n

\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"Bu deneyin ilk a\u015famas\u0131nda, farkl\u0131 metanol konsantrasyonlar\u0131n\u0131n (50%, 70%, 100%) Jinlongdeng t\u0131bbi malzemelerinin ekstraksiyon verimlili\u011fi \u00fczerindeki etkisi ara\u015ft\u0131r\u0131lm\u0131\u015f ve 70% metanol su ekstraksiyonu yap\u0131ld\u0131\u011f\u0131nda pikin en y\u00fcksek oldu\u011fu bulunmu\u015ftur. Bu arada, k\u00fctle spektrometresi ko\u015fullar\u0131n\u0131n ara\u015ft\u0131r\u0131lmas\u0131 s\u0131ras\u0131nda, negatif iyon modundaki toplam iyon kromatogram\u0131n\u0131n daha kararl\u0131 oldu\u011fu ve pik \u015feklinin daha \u00e7ekici oldu\u011fu bulunmu\u015ftur. Bu nedenle, deney negatif iyon tarama modunda ger\u00e7ekle\u015ftirilmi\u015ftir.<\/p>\n

Bu \u00e7al\u0131\u015fma, 16 parti Jinlongdeng \u015fifal\u0131 bitkisinin parmak izi spektrumlar\u0131n\u0131 olu\u015fturdu, 38 ortak pik \u00e7\u0131kard\u0131 ve benzerlik 0.95'in \u00fczerindeydi, bu da farkl\u0131 k\u00f6kenlerden Jinlongdeng \u015fifal\u0131 bitkilerinin kalitesinin nispeten istikrarl\u0131 oldu\u011funu g\u00f6steriyor; Hem k\u00fcme analizi hem de PCA analizi, \u00f6rnekleri farkl\u0131 k\u00f6kenlere g\u00f6re k\u00fcmeleyebilir, bu da t\u0131bbi malzemelerin b\u00fcy\u00fcme ortam\u0131n\u0131n kalite farkl\u0131l\u0131klar\u0131na neden olan \u00f6nemli bir fakt\u00f6r oldu\u011funu g\u00f6sterir.<\/p>\n

Ayn\u0131 zamanda, bu \u00e7al\u0131\u015fma 16 parti Jin Deng fener ekstrakt\u0131n\u0131n Staphylococcus aureus ve Escherichia coli'ye kar\u015f\u0131 antibakteriyel aktivitesini belirlemi\u015ftir. \u0130lk kez, parmak izi spektrumlar\u0131 farmakolojik sonu\u00e7larla birle\u015ftirildi ve Jin Deng fenerinin spektral etkinlik ili\u015fkisini incelemek i\u00e7in gri korelasyon analizi kullan\u0131ld\u0131. Sonu\u00e7lar, Jinlongdeng'in antibakteriyel aktivitesinin birden fazla bile\u015fenin birle\u015fik etkisinin sonucu olabilece\u011fini g\u00f6stermi\u015ftir. Bunlar aras\u0131nda, antibakteriyel aktiviteye \u00f6nemli \u00f6l\u00e7\u00fcde katk\u0131da bulunan karakteristik piklerin (korelasyon \u2265 0.85) t\u00fcm\u00fc s\u00fckroz ya\u011f asidi esterlerinden olu\u015fur ve bu bile\u015fenlerin Jinlongdeng'in antibakteriyel aktivitesine \u00f6nemli bir katk\u0131s\u0131 oldu\u011funu g\u00f6sterir.<\/p>\n

Ge\u00e7mi\u015fte, akademisyenlerin Jinlongdeng \u00fczerine ara\u015ft\u0131rmalar\u0131 esas olarak asit ac\u0131 bile\u015fiklerin karma\u015f\u0131k kimyasal yap\u0131s\u0131na ve \u00f6nemli biyolojik aktivitesine odaklan\u0131rken, s\u00fckroz ya\u011f asidi esterlerinin \u00e7al\u0131\u015fmas\u0131n\u0131 ihmal etmi\u015ftir. S\u00fckroz ya\u011f asidi ester bile\u015fenlerinin yap\u0131sal \u00f6zellikleri, s\u00fckrozun ana \u00e7ekirdek g\u00f6revi g\u00f6rmesi ve ester ba\u011flar\u0131 yoluyla bir veya daha fazla ya\u011f asidi zinciri par\u00e7as\u0131na ba\u011flanmas\u0131d\u0131r. Modern farmakolojik ara\u015ft\u0131rmalar, bu t\u00fcr bile\u015fiklerin \u00f6nemli antibakteriyel, anti-enflamatuar, hipoglisemik, anti-t\u00fcm\u00f6r, b\u00f6cek \u00f6ld\u00fcr\u00fcc\u00fc ve di\u011fer aktivitelere sahip oldu\u011funu g\u00f6stermi\u015ftir. Ayn\u0131 zamanda, s\u00fckroz ya\u011f asidi esterleri iyi biyolojik par\u00e7alanabilirli\u011fe ve toksikolojik \u00f6zelliklere sahiptir, bu nedenle uygulama beklentileri geni\u015ftir ve dikkatimizi \u00e7ekmeye de\u011ferdir.<\/p>\n

\u00d6zetle, bu \u00e7al\u0131\u015fma Lonicera japonica ekstrakt\u0131n\u0131n parmak izi spektrumunu ba\u015far\u0131yla olu\u015fturmu\u015f ve spektral etki ili\u015fkisini ilk kez incelemi\u015ftir. Sonu\u00e7lar, s\u00fckroz ya\u011f asidi esteri bile\u015fenlerinin Lonicera japonica'n\u0131n antibakteriyel aktivitesi ile y\u00fcksek oranda ili\u015fkili oldu\u011funu g\u00f6stermi\u015ftir. Bununla birlikte, benzersiz yap\u0131s\u0131 nedeniyle, bu deneyde k\u00fctle spektrometresi yoluyla yap\u0131s\u0131n\u0131n derinlemesine analizini yapmak m\u00fcmk\u00fcn olmam\u0131\u015ft\u0131r. Gelecekte, Jin Deng'deki bu t\u00fcr bir bile\u015fen izole edilecek ve antibakteriyel aktivitesini do\u011frulamak i\u00e7in safla\u015ft\u0131r\u0131lacakt\u0131r. Buna dayanarak, Jin Deng'in antibakteriyel aktivitesi ve etki mekanizmas\u0131 hakk\u0131nda daha fazla ara\u015ft\u0131rma i\u00e7in referans sa\u011flamak \u00fczere yap\u0131-aktivite ili\u015fkisi \u00f6zetlenecektir.<\/p>","protected":false},"excerpt":{"rendered":"

UPLC-Q-Orbitrap HRMS fingerprint and spectrum effect relationship of antibacterial activity of Jinlongdeng Jin Deng Deng, also known as Suanjiang or Red Maiden, is the dried or fruiting calyx of Physalis alkekengi L. var. franchetii (Mast.) Makino, a plant in the Solanaceae family. Sour and bitter in taste, cold in nature, belongs to the lung and […]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"\nUPLC-Q-Orbitrap HRMS fingerprint and spectrum effect relationship of antibacterial activity of Jinlongdeng - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/tr\/uplc-q-orbitrap-hrms\/\" \/>\n<meta property=\"og:locale\" content=\"tr_TR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"UPLC-Q-Orbitrap HRMS fingerprint and spectrum effect relationship of antibacterial activity of Jinlongdeng - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"UPLC-Q-Orbitrap HRMS fingerprint and spectrum effect relationship of antibacterial activity of Jinlongdeng Jin Deng Deng, also known as Suanjiang or Red Maiden, is the dried or fruiting calyx of Physalis alkekengi L. var. franchetii (Mast.) 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