{"id":5358,"date":"2024-08-04T18:33:21","date_gmt":"2024-08-04T18:33:21","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=5358"},"modified":"2024-08-04T18:33:21","modified_gmt":"2024-08-04T18:33:21","slug":"gc-ms","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/tr\/gc-ms\/","title":{"rendered":"Shanyinhua ya\u011f\u0131n\u0131n GC-MS ve LC-MS ile tam bile\u015fen karakterizasyonu ve in vitro anti-enflamatuar ve antioksidan aktiviteleri"},"content":{"rendered":"

Shanyinhua ya\u011f\u0131n\u0131n GC-MS ve LC-MS ile tam bile\u015fen karakterizasyonu ve in vitro anti-enflamatuar ve antioksidan aktiviteleri
\nDa\u011f G\u00fcm\u00fc\u015f \u00c7i\u00e7e\u011fi, Lonicera macranthoides Hand- Mazz.\u3001 Lonicera hypoglauca Miq., Lonicera hypoglauca Miq., Lonicera hypoglauca Miq., Lonicera hypoglauca Miq. bitkisi ile Lonicera ailesinin bir \u00fcyesidir, Lonicera hypoglauca Miq., Lonicera hypoglauca Miq., Lonicera hypoglauca Miq Lonicera confusa DC veya Lonicera fulvotontosa'n\u0131n yeni a\u00e7m\u0131\u015f \u00e7i\u00e7ekleri ile kurutulmu\u015f \u00e7i\u00e7ek tomurcuklar\u0131 veya \u00e7i\u00e7ekleri Hsu et S. G\u00fcney \u00c7in'de C. Cheng. Shanyinhua esas olarak d\u0131\u015f r\u00fczgar \u0131s\u0131s\u0131 veya salg\u0131n ate\u015fin neden oldu\u011fu \u0131s\u0131, k\u0131zar\u0131kl\u0131k, a\u011fr\u0131 ve \u015fi\u015flik ile karakterize bakteri ve vir\u00fcslerin neden oldu\u011fu erken enfeksiyonlar\u0131, yaralar\u0131, apseleri, \u00e7\u0131banlar\u0131 ve \u015fi\u015flikleri tedavi etmek i\u00e7in kullan\u0131l\u0131r. Han\u0131meli, geleneksel \u00c7in patentli ila\u00e7lar\u0131n ve basit preparatlar Vitamin C Yinqiao Tabletleri, So\u011fuk \u00d6ks\u00fcr\u00fck \u015eurubu ve Bile\u015fik Daqingye Kar\u0131\u015f\u0131m\u0131n\u0131n ana bile\u015fenidir. Buna ek olarak, da\u011f g\u00fcm\u00fc\u015f\u00fc \u00e7i\u00e7ekleri genellikle \u00e7ay ve \u015farap gibi \u00e7e\u015fitli sa\u011fl\u0131k \u00fcr\u00fcnlerinin \u00fcretiminde hammadde olarak kullan\u0131l\u0131r.
\n\u00c7ok say\u0131da bitki kimyas\u0131 \u00e7al\u0131\u015fmas\u0131 Shanyinhua'n\u0131n flavonoidler, organik asitler, iridoidler, saponinler ve ya\u011f i\u00e7erdi\u011fini g\u00f6stermi\u015ftir. Bununla birlikte, ya\u011f\u0131n\u0131n kimyasal bile\u015fimi ve biyolojik aktivitesinin derinlemesine incelenmesine ili\u015fkin s\u0131n\u0131rl\u0131 literat\u00fcr bulunmaktad\u0131r. Ara\u015ft\u0131rma y\u00f6ntemleri a\u00e7\u0131s\u0131ndan bak\u0131ld\u0131\u011f\u0131nda, rapor edilen literat\u00fcr a\u011f\u0131rl\u0131kl\u0131 olarak gaz kromatografisi-k\u00fctle spektrometresi (GC-MS) ve gaz kromatografisi-k\u00fctle spektrometresi (HS-SPME-GC-MS) teknikleri ile birle\u015ftirilmi\u015f tepe bo\u015flu\u011fu kat\u0131 faz ekstraksiyonunu benimsemektedir. Ekstraksiyon y\u00f6ntemlerinden, ultrason, mikrodalga, Soxhlet ekstraksiyonu ve buhar distilasyonunun a\u011f\u0131rl\u0131kl\u0131 olarak kullan\u0131ld\u0131\u011f\u0131 sonucuna var\u0131labilir. Ekstraksiyon y\u00f6ntemlerinin geni\u015fli\u011fi a\u00e7\u0131s\u0131ndan bak\u0131ld\u0131\u011f\u0131nda, ya\u011f elde etmek i\u00e7in CO2 s\u00fcperkritik ekstraksiyonu \u00fczerine nispeten az ara\u015ft\u0131rma vard\u0131r. Tespit y\u00f6ntemlerinin kapsaml\u0131l\u0131\u011f\u0131 a\u00e7\u0131s\u0131ndan bak\u0131ld\u0131\u011f\u0131nda, ya\u011fdaki orta polariteli maddelerin tespitinde eksiklik vard\u0131r. Buna ek olarak, Shanyinhua ya\u011f\u0131 iyi bir biyolojik aktiviteye sahiptir ve daha fazla geli\u015ftirme ve ara\u015ft\u0131rmaya de\u011ferdir.
\nBu nedenle, bu \u00e7al\u0131\u015fma da\u011f g\u00fcm\u00fc\u015f \u00e7i\u00e7e\u011fi ya\u011f\u0131n\u0131 ara\u015ft\u0131rma nesnesi olarak al\u0131r ve da\u011f g\u00fcm\u00fc\u015f \u00e7i\u00e7e\u011fi ya\u011f\u0131 elde etmek i\u00e7in CO2 s\u00fcperkritik ekstraksiyonu uygular; Metilasyon ve metilasyonsuzlu\u011fu GC-MS ile birle\u015ftirerek ya\u011fdaki u\u00e7ucu ve k\u00fc\u00e7\u00fck polar bile\u015fenleri analiz eder; Standart bir spektral k\u00fct\u00fcphane ile birlikte ultra y\u00fcksek performansl\u0131 s\u0131v\u0131 kromatografi-k\u00fctle spektrometresi (UHPLC-QE-MS) ile ya\u011fdaki orta polarite bile\u015fenlerinin tan\u0131mlanmas\u0131. Shanyinhua'n\u0131n kimyasal bile\u015fimini kapsaml\u0131 bir \u015fekilde analiz etmek i\u00e7in. Ayn\u0131 zamanda bu \u00e7al\u0131\u015fma, Lonicera japonica ya\u011f\u0131n\u0131n antioksidan ve anti-inflamatuar aktivitelerini de\u011ferlendirmek i\u00e7in in vitro antioksidan y\u00f6ntemleri ve in vitro h\u00fccre anti-inflamatuar deneylerini kullanarak, daha fazla geli\u015ftirilmesi ve kullan\u0131lmas\u0131 i\u00e7in bilimsel rehberlik sa\u011flam\u0131\u015ft\u0131r.<\/p>\n

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\nBu \u00e7al\u0131\u015fmada, lipopolisakkarit (LPS) taraf\u0131ndan ind\u00fcklenen bir h\u00fccre iltihab\u0131 modeli kullan\u0131larak Lonicera japonica ya\u011f\u0131n\u0131n in vitro anti-enflamatuar ve antioksidan aktiviteleri de\u011ferlendirilmi\u015f ve GC-MS ve UHPLC-QE-MS'ye dayal\u0131 olarak Lonicera japonica ya\u011f\u0131n\u0131n tam bile\u015fen analizi yap\u0131lm\u0131\u015ft\u0131r. Bu makalede Zhongshan G\u00fcm\u00fc\u015f \u00c7i\u00e7ek Ya\u011f\u0131n\u0131n ekstraksiyonu, iyi \u00e7\u00f6z\u00fcc\u00fc \u00e7\u00f6z\u00fcn\u00fcrl\u00fc\u011f\u00fcne, d\u00fc\u015f\u00fck ekstraksiyon s\u0131cakl\u0131\u011f\u0131na, b\u00fcy\u00fck g\u00fcvenli\u011fe sahip olan ve t\u0131bbi malzemelerdeki aktif bile\u015fenlerin oksidatif ayr\u0131\u015fmas\u0131n\u0131 etkili bir \u015fekilde \u00f6nleyebilen s\u00fcperkritik CO2 ekstraksiyon y\u00f6ntemini benimser. GC-MS analizi yoluyla, Shanyinhua ya\u011f\u0131nda esas olarak ya\u011f asitleri (metil palmitat, metil linoleat, vb.), steroller (\u03b3 - sitosterol, stigmasterol, vb.) ve alkanlar (skualan, n-tetradekan, vb.) dahil olmak \u00fczere toplam 66 bile\u015fik tan\u0131mlanm\u0131\u015ft\u0131r. Ara\u015ft\u0131rmalar, linoleik asit gibi \u00e7oklu doymam\u0131\u015f ya\u011f asitlerinin, enflamasyona neden olan arac\u0131lar\u0131n ve sitokinlerin pro-enflamatuar etkilerini engelleyebildi\u011fini ve enflamasyonla ilgili akut ve kronik hastal\u0131klar\u0131 hafifletebildi\u011fini; stigmasterol gibi sterol bile\u015fiklerinin lipid peroksidasyon reaksiyonlar\u0131n\u0131 etkili bir \u015fekilde geciktirebildi\u011fini, ya\u011f asidi de\u011ferinin ve peroksit de\u011ferinin artmas\u0131n\u0131 engelledi\u011fini ve dibutil hidroksitoluen (BHT) ve C vitamininden \u00f6nemli \u00f6l\u00e7\u00fcde daha iyi antioksidan etkilere sahip oldu\u011funu g\u00f6stermi\u015ftir. UHPLC-QE-MS arac\u0131l\u0131\u011f\u0131yla, pozitif ve negatif iyon modlar\u0131nda s\u0131ras\u0131yla 142 ve 83 bile\u015fik tan\u0131mlanm\u0131\u015ft\u0131r; bunlar\u0131n ba\u015fl\u0131calar\u0131 terpenler, kumarinler ve t\u00fcrevleri, fenilpropanoidler, alkaloidler, flavonoidler ve di\u011fer bile\u015fiklerdir. Literat\u00fcr raporlar\u0131na g\u00f6re, D2 vitamini ve ursolik asit gibi terpenoidler IL-6 ve TNF - \u03b1 ekspresyonunu in vivo ve in vitro olarak azaltabilir, anti-enflamatuar etkiler g\u00f6sterebilir ve \u00f6nemli in vitro antioksidan kapasiteye sahiptir. Naringin ve Brezilya hematoksilini gibi flavonoidler, LPS taraf\u0131ndan ind\u00fcklenen RAW 264.7 h\u00fccrelerinde NO, PGE2 ve TNF - \u03b1 gibi enflamatuar fakt\u00f6rlerin sal\u0131n\u0131m\u0131n\u0131 inhibe edebilir ve anti-enflamatuar, antibakteriyel, antioksidan ve anti-t\u00fcm\u00f6r etkileri gibi farmakolojik etkiler g\u00f6sterebilir. \u00c7al\u0131\u015fmalar, ferulik asit gibi fenilpropanoid bile\u015fiklerin hidrojen peroksit, peroksinitrit ve s\u00fcperoksit radikallerini etkili bir \u015fekilde temizleyebildi\u011fini g\u00f6stermi\u015ftir. Bu nedenle, yukar\u0131daki sonu\u00e7lardan Shanyinhua ya\u011f\u0131n\u0131n antioksidan ve anti-inflamatuar aktivitelere sahip oldu\u011fu \u00e7\u0131kar\u0131labilir.<\/p>\n

Zeng ve arkada\u015flar\u0131 han\u0131meli ve da\u011f g\u00fcm\u00fc\u015f\u00fc \u00e7i\u00e7e\u011fi \u00f6zlerinin farelerde ksilen taraf\u0131ndan ind\u00fcklenen kulak \u015fi\u015fmesi derecesini \u00f6nemli \u00f6l\u00e7\u00fcde inhibe edebildi\u011fini ve ayr\u0131ca in vitro h\u00fccre modelinde RAW 264.7 h\u00fccrelerinde LPS taraf\u0131ndan ind\u00fcklenen inflamatuar sitokinlerin sal\u0131n\u0131m\u0131n\u0131 inhibe edebildi\u011fini bulmu\u015ftur. Her ikisi de in vitro ve in vivo olarak iyi anti-enflamatuar etkilere sahiptir. Zou ve arkada\u015flar\u0131, Lonicera japonica'n\u0131n farkl\u0131 ekstraktlar\u0131n\u0131n in vitro anti-enflamatuar aktivitesini de\u011ferlendirmek i\u00e7in LPS ile ind\u00fcklenen bir h\u00fccre enflamasyon modeli kullanm\u0131\u015flard\u0131r. Lonicera japonica'n\u0131n farkl\u0131 ekstraktlar\u0131 farkl\u0131 derecelerde anti-enflamatuar aktivite g\u00f6stermi\u015ftir. Bu \u00e7al\u0131\u015fma, Lonicera japonica ya\u011f\u0131n\u0131n anti-enflamatuar aktivitesini LPS ile ind\u00fcklenen h\u00fccresel enflamasyon modeli kullanarak de\u011ferlendirmi\u015f ve Lonicera japonica ya\u011f\u0131n\u0131n \u00f6nemli bir anti-enflamatuar aktiviteye sahip oldu\u011funu ve \u00f6nceki \u00e7al\u0131\u015fmalara benzer \u015fekilde doza ba\u011fl\u0131 bir model g\u00f6sterdi\u011fini ortaya koymu\u015ftur.<\/p>\n

Asl\u0131nda, anti-enflamatuar deneyler Shanyinhua ya\u011f\u0131n\u0131n enflamatuar fakt\u00f6rlerin sal\u0131n\u0131m\u0131n\u0131 \u00f6nemli \u00f6l\u00e7\u00fcde engelleyebildi\u011fini ve anti-enflamatuar etkiler sergiledi\u011fini g\u00f6stermi\u015ftir. Yukar\u0131daki sonu\u00e7lar, Shanyinhua ya\u011f\u0131n\u0131n iyi anti-enflamatuar etkilere sahip do\u011fal bir aktif madde oldu\u011funu ve iyi bir geli\u015fim ve uygulama de\u011ferine sahip oldu\u011funu g\u00f6stermektedir. Bu \u00e7al\u0131\u015fman\u0131n antioksidan sonu\u00e7lar\u0131, Shanyinhua ya\u011f\u0131n\u0131n ABTS ve DPPH serbest radikallerini temizleme yetene\u011finin g\u00fc\u00e7l\u00fc olmad\u0131\u011f\u0131n\u0131 g\u00f6stermektedir, bu da bu y\u00f6ntemle ekstrakte edilen ya\u011fdaki d\u00fc\u015f\u00fck antioksidan madde i\u00e7eri\u011finden kaynaklan\u0131yor olabilir. Ayr\u0131ca yeni ekstraksiyon y\u00f6ntemleri geli\u015ftirebilece\u011fimizi de g\u00f6stermektedir. Bu nedenle, bu \u00e7al\u0131\u015fman\u0131n kapsaml\u0131 kimyasal analiz sonu\u00e7lar\u0131 ve \u00f6n aktivite de\u011ferlendirmesi, Shanyinhua'n\u0131n hem t\u0131bbi hem de yenilebilir uygulamalarda kapsaml\u0131 geli\u015fimi i\u00e7in bir temel sa\u011flayabilir.<\/p>","protected":false},"excerpt":{"rendered":"

Full component characterization and in vitro anti-inflammatory and antioxidant activities of Shanyinhua oil by GC-MS and LC-MS Mountain Silver Flower is a member of the Lonicera family, with the plant Lonicera macranthoides Hand- Mazz.\u3001 Lonicera hypoglauca Miq., Lonicera hypoglauca Miq., Lonicera hypoglauca Miq., Lonicera hypoglauca Miq., Lonicera hypoglauca Miq., Lonicera hypoglauca Miq., Lonicera hypoglauca Miq […]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"\nFull component characterization and in vitro anti-inflammatory and antioxidant activities of Shanyinhua oil by GC-MS and LC-MS - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/tr\/gc-ms\/\" \/>\n<meta property=\"og:locale\" content=\"tr_TR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Full component characterization and in vitro anti-inflammatory and antioxidant activities of Shanyinhua oil by GC-MS and LC-MS - 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