{"id":5331,"date":"2024-08-04T18:25:08","date_gmt":"2024-08-04T18:25:08","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=5331"},"modified":"2024-08-04T18:25:08","modified_gmt":"2024-08-04T18:25:08","slug":"lobelia","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/tr\/lobelia\/","title":{"rendered":"HPLC parmak izi ve i\u00e7erik tayinine dayal\u0131 olarak yar\u0131m lotus kaynatma par\u00e7alar\u0131 ile standart kaynatma aras\u0131ndaki dozaj de\u011ferlerinin transfer yasas\u0131 \u00fczerine \u00e7al\u0131\u015fma"},"content":{"rendered":"<p>HPLC parmak izi ve i\u00e7erik tayinine dayal\u0131 olarak yar\u0131m lotus kaynatma par\u00e7alar\u0131 ile standart kaynatma aras\u0131ndaki dozaj de\u011ferlerinin transfer yasas\u0131 \u00fczerine \u00e7al\u0131\u015fma<br \/>\nCampanulaceae familyas\u0131ndan kurutulmu\u015f b\u00fct\u00fcn bir bitki olan Lobelia chinensis Lour. \u0131s\u0131y\u0131 temizleme, detoksifiye etme, di\u00fcrez ve \u015fi\u015fli\u011fi azaltma etkilerine sahiptir. Ara\u015ft\u0131rmalar, yar\u0131m lotusun esas olarak antibakteriyel, antiviral, anti-t\u00fcm\u00f6r, anti-enflamatuar ve analjezik etkiler gibi farmakolojik etkilere sahip flavonoidler, alkaloidler ve kumarinler i\u00e7erdi\u011fini g\u00f6stermi\u015ftir. Yar\u0131m nil\u00fcfer farmakopede yayg\u0131n olarak kullan\u0131lan bir geleneksel \u00c7in ilac\u0131d\u0131r, ancak farmakope kapsam\u0131ndaki yar\u0131m nil\u00fcfer t\u0131bbi malzemeleri i\u00e7in kalite standartlar\u0131 yaln\u0131zca \u00f6zellikleri, tan\u0131mlama, inceleme ve ekstraksiyon \u00f6\u011felerini i\u00e7erir ve kalite standartlar\u0131 hen\u00fcz tamamlanmam\u0131\u015ft\u0131r. \u015eu anda, yar\u0131m lotusun kalite kontrol ara\u015ft\u0131rmas\u0131 ve bile\u015fen analizi hakk\u0131nda hala nispeten az say\u0131da rapor bulunmaktad\u0131r.<\/p>\n<p>Geleneksel \u00c7in t\u0131bb\u0131, \u00c7in t\u0131bb\u0131n\u0131n etkinli\u011finin maddi temeli olan ana ila\u00e7 \u015fekli olarak \u00e7o\u011funlukla kaynatma kullan\u0131r. Binlerce y\u0131ll\u0131k klinik uygulamadan sonra, \u00c7in t\u0131bb\u0131 standart dekoksiyonlar\u0131 i\u00e7in kalite standartlar\u0131n\u0131n olu\u015fturulmas\u0131, \u00c7in t\u0131bb\u0131 form\u00fcl gran\u00fclleri gibi modern \u00c7in t\u0131bb\u0131 form\u00fclasyonlar\u0131 i\u00e7in kalite standartlar\u0131n\u0131n olu\u015fturulmas\u0131 i\u00e7in \u00f6nemli referanslar sa\u011flayabilir. \u015eu anda, yar\u0131m nil\u00fcferin standart kaynat\u0131lmas\u0131na ili\u015fkin herhangi bir ara\u015ft\u0131rma raporu bulunmamaktad\u0131r. Bu makalenin yazar\u0131, \"Geleneksel \u00c7in T\u0131bb\u0131 Form\u00fcl Gran\u00fcllerinin Kalite Kontrol\u00fc ve Standart Geli\u015ftirilmesi i\u00e7in Teknik Gereklilikler\" (bundan b\u00f6yle \"Teknik Gereklilikler\" olarak an\u0131lacakt\u0131r) uyar\u0131nca Banbianlian form\u00fcl gran\u00fclleri i\u00e7in ulusal standard\u0131 incelemi\u015f ve standart kaynatman\u0131n temel kalite g\u00f6stergelerini incelemi\u015ftir: merhem verimi, ekstrakt, g\u00f6sterge bile\u015fenlerinin i\u00e7eri\u011fi ve aktar\u0131m oran\u0131 ve parmak izi spektrumu. \u00c7al\u0131\u015fma, Banbianlian kaynatma par\u00e7alar\u0131ndan standart kaynatmaya miktar de\u011ferinin aktar\u0131m yasas\u0131n\u0131 incelemi\u015f ve Banbianlian form\u00fcl gran\u00fclleri ve ilgili preparatlar i\u00e7in kalite standartlar\u0131n\u0131n geli\u015ftirilmesi i\u00e7in referans sa\u011flam\u0131\u015ft\u0131r.<\/p>\n<p>&nbsp;<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-5332\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-137.png\" alt=\"\" width=\"783\" height=\"233\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-137.png 783w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-137-768x229.png 768w\" sizes=\"(max-width: 783px) 100vw, 783px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-5333\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/2-124.png\" alt=\"\" width=\"782\" height=\"257\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/2-124.png 782w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/2-124-768x252.png 768w\" sizes=\"(max-width: 782px) 100vw, 782px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-5334\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/3-118.png\" alt=\"\" width=\"765\" height=\"295\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-5335\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/4-106.png\" alt=\"\" width=\"767\" height=\"300\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-5336\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/5-95.png\" alt=\"\" width=\"760\" height=\"634\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-5337\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/6-88.png\" alt=\"\" width=\"761\" height=\"310\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-5338\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/7-79.png\" alt=\"\" width=\"755\" height=\"376\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-5339\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/8-73.png\" alt=\"\" width=\"782\" height=\"195\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/8-73.png 782w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/8-73-768x192.png 768w\" sizes=\"(max-width: 782px) 100vw, 782px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-5340\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/9-66.png\" alt=\"\" width=\"778\" height=\"284\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/9-66.png 778w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/9-66-768x280.png 768w\" sizes=\"(max-width: 778px) 100vw, 778px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-5341\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/10-54.png\" alt=\"\" width=\"767\" height=\"344\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-5342\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/11-48.png\" alt=\"\" width=\"764\" height=\"281\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-5343\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/12-41.png\" alt=\"\" width=\"742\" height=\"302\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-5344\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/13-36.png\" alt=\"\" width=\"716\" height=\"296\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-5345\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/14-31.png\" alt=\"\" width=\"773\" height=\"651\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/14-31.png 773w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/14-31-768x647.png 768w\" sizes=\"(max-width: 773px) 100vw, 773px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-5346\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/15-23.png\" alt=\"\" width=\"771\" height=\"479\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/15-23.png 771w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/15-23-768x477.png 768w\" sizes=\"(max-width: 771px) 100vw, 771px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-5347\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/16-18.png\" alt=\"\" width=\"780\" height=\"251\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/16-18.png 780w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/16-18-768x247.png 768w\" sizes=\"(max-width: 780px) 100vw, 780px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-5348\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/17-15.png\" alt=\"\" width=\"778\" height=\"430\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/17-15.png 778w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/17-15-768x424.png 768w\" sizes=\"(max-width: 778px) 100vw, 778px\" \/>Yar\u0131m lotus nispeten hafif bir dokuya sahiptir ve kaynatma i\u015flemi s\u0131ras\u0131nda b\u00fcy\u00fck miktarda bile\u015fen ta\u015far, bu da parmak izi spektrumlar\u0131n\u0131n olu\u015fturulmas\u0131n\u0131n zorlu\u011funu art\u0131r\u0131r. Parmak izi spektrumlar\u0131n\u0131n olu\u015fturulmas\u0131 s\u00fcrecinde, bilgi i\u00e7eri\u011fi, kromatografik pik yan\u0131t\u0131 ve ay\u0131rma etkisi dikkate al\u0131narak \u00fc\u00e7 mobil faz sistemi ara\u015ft\u0131r\u0131lm\u0131\u015ft\u0131r: metanol-0.1% fosforik asit \u00e7\u00f6zeltisi, asetonitril-0.1% fosforik asit \u00e7\u00f6zeltisi ve asetonitril-0.1mol\/L potasyum dihidrojen fosfat \u00e7\u00f6zeltisi. Son olarak, asetonitril-0.1mol\/L potasyum dihidrojen fosfat \u00e7\u00f6zeltisi se\u00e7ilmi\u015ftir. Tam dalga boyu tarama modu benimsenerek parmak izi spektrumunun optimum absorpsiyon dalga boyu belirlenmi\u015ftir. \"Parmak izi spektrumundaki ortak piklerin toplam pik alan\u0131\/\u00f6rnek boyutu\" test \u00e7\u00f6zeltisi i\u00e7in en iyi haz\u0131rlama y\u00f6ntemini se\u00e7mek i\u00e7in g\u00f6sterge olarak kullan\u0131lm\u0131\u015ft\u0131r. Olu\u015fturulan parmak izi spektrum y\u00f6ntemi sistematik metodoloji ile do\u011frulanm\u0131\u015f ve gereksinimleri kar\u015f\u0131lam\u0131\u015ft\u0131r. \u00c7in Farmakopesi'nin 2020 bask\u0131s\u0131nda, \"yar\u0131m lotus\" kategorisi alt\u0131nda i\u00e7erik belirleme g\u00f6stergeleri bulunmamaktad\u0131r. \"Hong Kong \u00c7in Bitkisel \u0130la\u00e7 Standartlar\u0131\", Zeng ve di\u011fer ara\u015ft\u0131rma sonu\u00e7lar\u0131na at\u0131fta bulunarak, standart kaynatmalarda de\u011fer aktar\u0131m\u0131 \u00e7al\u0131\u015fmas\u0131na rehberlik etmek i\u00e7in diosmin ve paeoniflorin aktif bile\u015fenlerinin i\u00e7eri\u011fini belirlemeye y\u00f6nelik bir y\u00f6ntem olu\u015fturulmu\u015ftur. \u00dcretim alan\u0131n\u0131n ara\u015ft\u0131r\u0131lmas\u0131na g\u00f6re, yar\u0131m lotus esas olarak Guizhou, Anhui, Jiangxi, Hunan, Hubei, Henan ve di\u011fer yerlerde, ana kaynak olarak yabani kaynaklarla \u00fcretilmektedir. Numunelerin temsil kabiliyetini sa\u011flamak i\u00e7in personelimiz, standart \u00e7orba ara\u015ft\u0131rmas\u0131n\u0131n ihtiya\u00e7lar\u0131n\u0131 kar\u015f\u0131lamak \u00fczere numune toplamak i\u00e7in yar\u0131m lotusun ana \u00fcretim alanlar\u0131n\u0131n derinliklerine gitti. Standart kaynatman\u0131n ekstraksiyon oran\u0131, ekstrakt ve i\u00e7erik belirleme sonu\u00e7lar\u0131ndan, Banbianlian standart kaynatmas\u0131n\u0131n 21 partisinde ekstraksiyon oran\u0131, ekstrakt ve diosmin ve paeoniflorin i\u00e7eri\u011finde belirli farkl\u0131l\u0131klar oldu\u011fu g\u00f6r\u00fclebilir. Maksimum ekstraksiyon oran\u0131 ve ekstrakt minimum de\u011ferin yakla\u015f\u0131k 1,6 kat\u0131d\u0131r ve maksimum diosmin ve paeoniflorin i\u00e7eri\u011fi minimum de\u011ferin 2-4 kat\u0131d\u0131r. Bu fark, \u015fifal\u0131 bitkilerin k\u00f6keni, hasat zaman\u0131 ve i\u015fleme y\u00f6ntemlerindeki farkl\u0131l\u0131klarla ilgili olabilir.<br \/>\n\u00c7in Farmakopesi'nin 2020 bask\u0131s\u0131nda Yar\u0131m Lotus \u015fifal\u0131 bitkisi i\u00e7in herhangi bir i\u00e7erik belirleme g\u00f6stergesi bulunmamaktad\u0131r. Flavonoidler, Yar\u0131m Lotus'ta \u00e7ok \u00f6nemli bir bile\u015fik s\u0131n\u0131f\u0131d\u0131r ve anti-kanser tedavisi i\u00e7in ana aktif bile\u015fenlerdir. Bunlar aras\u0131nda Diosmin, ven\u00f6z ge\u00e7irgenli\u011fi ve mikrosirk\u00fclasyonu iyile\u015ftirme etkisine sahip bir flavonoid glikozit bile\u015fi\u011fidir. Montessorin ve Diosmin \u00e7ok benzer yap\u0131lara ve Half Lotus'ta y\u00fcksek i\u00e7eri\u011fe sahiptir. Bu nedenle, Diosmin ve Montessorin, belirli bir \u00f6zg\u00fcll\u00fc\u011fe ve pratik \u00f6neme sahip olan \u015fifal\u0131 bitkiler ve kaynatma par\u00e7alar\u0131 i\u00e7in i\u00e7erik belirleme g\u00f6stergeleri olarak kullan\u0131l\u0131r. Bununla birlikte, transfer oran\u0131n\u0131n hesaplama sonu\u00e7lar\u0131na g\u00f6re, diosmin ve mogrosidin kaynatma par\u00e7alar\u0131ndan standart kaynatmaya transfer oranlar\u0131n\u0131n her ikisi de 10%'den azd\u0131r, bu da suda \u00e7\u00f6z\u00fcn\u00fcrl\u00fcklerinin ideal olmad\u0131\u011f\u0131n\u0131 g\u00f6sterir. Parmak izi spektrumundaki ortak piklerin oran\u0131ndan, parmak izi spektrumunun toplam pik alan\u0131ndaki diosmin ve mogrosid kromatografik piklerinin oran\u0131n\u0131n kaynatma par\u00e7alar\u0131ndan standart kaynatmaya do\u011fru \u00f6nemli \u00f6l\u00e7\u00fcde azald\u0131\u011f\u0131 a\u00e7\u0131k\u00e7a g\u00f6r\u00fclebilir. Yar\u0131m lotusun parmak izi spektrumundan belirlenen di\u011fer bile\u015fenler i\u00e7in bilinen konsantrasyonlara sahip yasal referans standartlar\u0131n\u0131n eksikli\u011fi g\u00f6z \u00f6n\u00fcne al\u0131nd\u0131\u011f\u0131nda, suda \u00e7\u00f6z\u00fcnen bile\u015fenlerin transferini analiz etmek i\u00e7in daha fazla ara\u015ft\u0131rmaya ihtiya\u00e7 vard\u0131r. PCA sonu\u00e7lar\u0131na g\u00f6re, Banbianlian kaynatmas\u0131ndan standart kaynatmaya kadar ortak piklerin g\u00f6receli pik alan\u0131nda \u00f6nemli bir de\u011fi\u015fiklik oldu\u011fu g\u00f6r\u00fclebilir. OPLS-DA, her bir ortak pik taraf\u0131ndan temsil edilen kimyasal bile\u015fenlerin transfer oranlar\u0131ndaki farkl\u0131l\u0131klarla ilgili oldu\u011fu d\u00fc\u015f\u00fcn\u00fclen pik 9, pik 7, pik 5, pik 4 ve pik 1 olmak \u00fczere ba\u011f\u0131l pik alan\u0131nda \u00f6nemli de\u011fi\u015fikliklere sahip be\u015f kromatografik pik tespit etmi\u015ftir.<br \/>\nBu \u00e7al\u0131\u015fmada, Banbianlian kaynatma par\u00e7alar\u0131ndan standart kaynatmaya de\u011fer aktar\u0131m s\u00fcrecini analiz etmek i\u00e7in de\u011ferlendirme g\u00f6stergeleri olarak ekstraksiyon oran\u0131, ekstrakt, parmak izi spektrumu, aktif bile\u015fenlerin i\u00e7eri\u011fi ve standart kaynatma aktar\u0131m oran\u0131 kullan\u0131lm\u0131\u015f ve Banbianlian form\u00fcl gran\u00fclleri ve ilgili preparatlar i\u00e7in kalite standartlar\u0131n\u0131n geli\u015ftirilmesi i\u00e7in referans sa\u011flanm\u0131\u015ft\u0131r.<\/p>","protected":false},"excerpt":{"rendered":"<p>Study on the transfer law of dosage values between half lotus decoction pieces and standard decoction based on HPLC fingerprint and content determination Lobelia chinensis Lour., a dried whole plant of the Campanulaceae family, has the effects of clearing heat, detoxifying, diuresis, and reducing swelling. Research has shown that half lotus mainly contains flavonoids, alkaloids, [&hellip;]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.0 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Study on the transfer law of dosage values between half lotus decoction pieces and standard decoction based on HPLC fingerprint and content determination - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/tr\/lobelia\/\" \/>\n<meta property=\"og:locale\" content=\"tr_TR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Study on the transfer law of dosage values between half lotus decoction pieces and standard decoction based on HPLC fingerprint and content determination - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"Study on the transfer law of dosage values between half lotus decoction pieces and standard decoction based on HPLC fingerprint and content determination Lobelia chinensis Lour., a dried whole plant of the Campanulaceae family, has the effects of clearing heat, detoxifying, diuresis, and reducing swelling. 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