{"id":4745,"date":"2024-08-04T15:20:55","date_gmt":"2024-08-04T15:20:55","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=4745"},"modified":"2024-08-04T15:20:55","modified_gmt":"2024-08-04T15:20:55","slug":"cistanche-deserticola","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/tr\/cistanche-deserticola\/","title":{"rendered":"HPLC-QQQ-MS teknolojisine dayal\u0131 olarak i\u015flemenin Cistanche deserticola'daki be\u015f aktif bile\u015fen i\u00e7eri\u011fi \u00fczerindeki etkisinin ara\u015ft\u0131r\u0131lmas\u0131"},"content":{"rendered":"<p>HPLC-QQQ-MS teknolojisine dayal\u0131 olarak Cistanche tubulosa'daki be\u015f aktif bile\u015fenin i\u00e7eri\u011fi \u00fczerindeki i\u015flemenin etkisinin ara\u015ft\u0131r\u0131lmas\u0131. Cistanche tubulosa, karaci\u011fer ve b\u00f6brekleri besleyen, \u00f6z\u00fc ve kan\u0131 besleyen, ba\u011f\u0131rsaklar\u0131 nemlendiren ve ba\u011f\u0131rsak hareketlerini te\u015fvik eden ve ya\u015flanmay\u0131 geciktiren de\u011ferli bir \u00c7in bitkisel ilac\u0131d\u0131r. B\u00f6brek yetmezli\u011fini, kad\u0131n k\u0131s\u0131rl\u0131\u011f\u0131n\u0131, kab\u0131zl\u0131\u011f\u0131 tedavi etmek, haf\u0131zay\u0131 geli\u015ftirmek, Alzheimer hastal\u0131\u011f\u0131n\u0131 tedavi etmek ve ba\u011f\u0131\u015f\u0131kl\u0131\u011f\u0131 art\u0131rmak i\u00e7in kullan\u0131l\u0131r. Cistanche deserticola'n\u0131n bile\u015fenleri, feniletanolik glikozitler, benzil alkol glikozitler, sikloheksen eter terpenler ve bunlar\u0131n glikozitleri, lignanlar ve bunlar\u0131n glikozaminoglikanlar\u0131 vb. dahil olmak \u00fczere \u00e7ok \u00e7e\u015fitlidir. Kapsaml\u0131 farmakolojik aktivitesi, i\u00e7erdi\u011fi biyoaktif bile\u015fenlerle yak\u0131ndan ili\u015fkilidir. \u00d6rne\u011fin, Cistanche deserticola'dan elde edilen polisakkaritler hidroksil radikallerini etkili bir \u015fekilde temizleyebilir ve \u00f6nemli antioksidan aktivite sergileyebilir. Verbascoside ve echinaceae glycoside gibi feniletanoid glikozitler, kan oksijen sat\u00fcrasyonunu art\u0131rarak ve v\u00fccut tolerans\u0131n\u0131 geli\u015ftirerek kronik y\u00fcksek irtifa hastal\u0131\u011f\u0131n\u0131 iyile\u015ftirebilir. Pinecone krizantem glikozitinin Parkinson hastal\u0131\u011f\u0131na kar\u015f\u0131 etkileri oldu\u011fu ara\u015ft\u0131rmalarla kan\u0131tlanm\u0131\u015ft\u0131r. Cistanche deserticola'n\u0131n aktif bile\u015fenlerini ve farmakolojik etkilerini daha fazla ara\u015ft\u0131rmak i\u00e7in, daha \u00f6nce bu t\u00fcr\u00fcn kimyasal bile\u015fimini ve farmakolojik etkilerini sistematik olarak \u00f6zetledik.<\/p>\n<p>Cistanche deserticola'n\u0131n belirgin ve \u00f6nemli farmakolojik aktivitesi nedeniyle, aktif bile\u015fenlerine nispeten daha fazla ilgi g\u00f6sterilmi\u015f ve i\u00e7erik belirleme ara\u015ft\u0131rmalar\u0131 \u00e7e\u015fitli \u015fekillerde ger\u00e7ekle\u015ftirilmi\u015ftir. Cistanche deserticola'daki farkl\u0131 polarite ve i\u00e7erik aral\u0131klar\u0131n\u0131n bile\u015fimini belirlemek i\u00e7in tandem e\u015fle\u015fmi\u015f ters fazl\u0131 hidrofilik etkile\u015fim kromatografisi tandem k\u00fctle spektrometrisi gibi farkl\u0131 ara\u015ft\u0131rma teknikleri kullan\u0131lm\u0131\u015ft\u0131r. Bir \u00e7al\u0131\u015fmada, Cistanche deserticola'daki feniletanoid glikozitlerin \u00e7oklu bile\u015fenlerini bir i\u015faretleyici kullanarak kar\u015f\u0131la\u015ft\u0131rmak ve analiz etmek i\u00e7in g\u00f6receli bir d\u00fczeltme fakt\u00f6r\u00fc hesaplama y\u00f6ntemi kullan\u0131lm\u0131\u015ft\u0131r. UV spektrofotometri, fenol s\u00fclf\u00fcrik asit y\u00f6ntemi ve y\u00fcksek performansl\u0131 s\u0131v\u0131 kromatografisi, ham ve i\u015flenmi\u015f Cistanche deserticola'daki toplam feniletanolik glikozitler, toplam polisakkaritler ve d\u00f6rt temsili bile\u015fendeki de\u011fi\u015fiklikleri analiz etmek i\u00e7in kullan\u0131lm\u0131\u015ft\u0131r. Bu ara\u015ft\u0131rma y\u00f6ntemlerinden baz\u0131lar\u0131 kesin olarak \u00f6l\u00e7\u00fclemezken, di\u011ferleri d\u00fc\u015f\u00fck hassasiyete sahiptir. Y\u00fcksek performansl\u0131 s\u0131v\u0131 kromatografisi tandem \u00fc\u00e7l\u00fc kuadrupol k\u00fctle spektrometresi (HPLC-QQQ-MS) tekni\u011fi d\u00fc\u015f\u00fck tespit limiti ve y\u00fcksek hassasiyete sahip oldu\u011fundan, Cistanche deserticola'n\u0131n karma\u015f\u0131k ve d\u00fc\u015f\u00fck bile\u015fen i\u00e7erikli \u00f6rneklerinin analizi i\u00e7in daha uygundur.<\/p>\n<p>\u00c7in t\u0131bb\u0131 i\u015fleme, geleneksel \u00c7in t\u0131bb\u0131nda yan etkilerin azalt\u0131lmas\u0131nda, ila\u00e7 etkinli\u011finin art\u0131r\u0131lmas\u0131nda, t\u0131bbi malzemelerin \u00f6zelliklerinin de\u011fi\u015ftirilmesinde ve hatta terap\u00f6tik etkilerin de\u011fi\u015ftirilmesinde \u00f6nemli bir rol oynayan benzersiz ve ay\u0131rt edici bir farmas\u00f6tik tekniktir. \u0130\u015fleme, geleneksel \u00c7in t\u0131bb\u0131n\u0131n temel farmakolojik madde temelini de\u011fi\u015ftirebilir ve b\u00f6ylece etkinli\u011fini art\u0131rabilir. Geleneksel \u00c7in t\u0131bb\u0131 teorisi, alkol i\u015flemenin b\u00f6brek tonland\u0131r\u0131c\u0131 ila\u00e7lar\u0131n etkinli\u011fini daha iyi art\u0131rabilece\u011fine inanmaktad\u0131r. Bu nedenle, Cistanche deserticola her zaman sar\u0131 \u015farap ile i\u015flenmi\u015f ve ila\u00e7 olarak kullan\u0131lm\u0131\u015ft\u0131r. \u0130\u015flemenin Cistanche deserticola'n\u0131n kimyasal temeli \u00fczerindeki etkisini daha iyi de\u011ferlendirmek i\u00e7in, i\u015flemeden \u00f6nce ve sonra Cistanche deserticola'n\u0131n kan bile\u015fenleri ve metabolomikleri \u00fczerinde sistematik bir \u00e7al\u0131\u015fma y\u00fcr\u00fctt\u00fck. \u0130\u015flemeden \u00f6nce ve sonra Cistanche deserticola'daki bile\u015fiklerin kalite belirte\u00e7lerinin tan\u0131mlanmas\u0131 ve taranmas\u0131na dayanarak, Cistanche deserticola'dan Cistanche glikozitleri F, verbascoside, izoergosterol glikozitler, 2 '- asetil verbascoside ve echinacoside dahil olmak \u00fczere be\u015f temsili bile\u015fik se\u00e7tik ve bunlar\u0131 kantitatif olarak analiz ettik. HPLCQQ-MS cihaz\u0131nda Cistanche deserticola'n\u0131n kimyasal i\u00e7eri\u011fini belirlemek i\u00e7in bir y\u00f6ntem olu\u015fturulmu\u015ftur. Be\u015f bile\u015fen aras\u0131nda, Cistanche deserticola glikozit F fenilpropanoid oligosakkaritler s\u0131n\u0131f\u0131na aitken, di\u011fer d\u00f6rd\u00fc feniletanoid glikozitlerdir ve hepsi \u00f6nemli farmakolojik aktiviteye sahiptir. Maorui \u00e7i\u00e7e\u011fi glikozidi ve pineal krizantem glikozidi de \u00c7in Farmakopesi'nde kalite kontrol referans standartlar\u0131 olarak kullan\u0131lmaktad\u0131r. Bu deney, Cistanche deserticola'n\u0131n kantitatif analiz perspektifinden sonraki i\u015fleme verimlili\u011fi ve kalite de\u011ferlendirmesi i\u00e7in teorik bir temel sa\u011flayacakt\u0131r.<\/p>\n<p>&nbsp;<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4748\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-89.png\" alt=\"\" width=\"764\" height=\"286\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4749\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/2-78.png\" alt=\"\" width=\"765\" height=\"600\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4750\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/3-73.png\" alt=\"\" width=\"766\" height=\"226\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4751\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/4-61.png\" alt=\"\" width=\"766\" height=\"646\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4752\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/5-59.png\" alt=\"\" width=\"765\" height=\"292\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4753\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/6-54.png\" alt=\"\" width=\"765\" height=\"469\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4754\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/7-51.png\" alt=\"\" width=\"765\" height=\"264\" \/>Bu makalede olu\u015fturulan HPLC-QQQ-MS y\u00f6ntemi esas olarak Cistanche deserticola'daki be\u015f kimyasal bile\u015fenin i\u015flenmeden \u00f6nce ve sonra belirlenmesi i\u00e7in kullan\u0131lm\u0131\u015ft\u0131r. Geleneksel \u00c7in t\u0131bb\u0131n\u0131n kalite standard\u0131 teorisi rehberli\u011finde, deney modern farmakolojik ara\u015ft\u0131rmalara dayanarak i\u00e7erik belirleme i\u00e7in bile\u015fenleri tarad\u0131 ve sonu\u00e7ta g\u00f6sterge bile\u015fenleri olarak Cistanche deserticola glikozit F, verbascoside, izoergosterol glikozit, 2 '- asetil verbascoside ve pineal krizantem glikozit se\u00e7ildi. Deney, \u00c7in Farmakopesi'nin 2020 bask\u0131s\u0131nda Cistanche deserticola'n\u0131n i\u00e7erik belirleme maddesi alt\u0131ndaki ekstraksiyon y\u00f6ntemini takip etti ve hem ham hem de i\u015flenmi\u015f Cistanche deserticola \u00fcr\u00fcnlerinden \u00f6rnekler \u00e7\u0131kard\u0131. Be\u015f kimyasal bile\u015fen i\u00e7in s\u0131v\u0131 faz ve k\u00fctle spektrometresi ko\u015fullar\u0131 optimize edilmi\u015ftir. Deneyde kullan\u0131lan y\u00fcksek performansl\u0131 s\u0131v\u0131 kromatografi tandem \u00fc\u00e7l\u00fc kuadrupol k\u00fctle spektrometresi, y\u00fcksek \u00e7\u00f6z\u00fcn\u00fcrl\u00fck, y\u00fcksek hassasiyet ve h\u0131zl\u0131 analiz avantajlar\u0131na sahiptir. Bile\u015fiklerin verimli bir \u015fekilde ayr\u0131lmas\u0131 ve taranmas\u0131, farkl\u0131 molek\u00fcllerin kantitatif analizi, son derece k\u00fc\u00e7\u00fck molek\u00fcllerin tespiti ve karma\u015f\u0131k kar\u0131\u015f\u0131k kimyasal bile\u015fenlerin verimli ve h\u0131zl\u0131 analizini yapabilmektedir. Bu nedenle, bu platform \u00fczerinde olu\u015fturulan ara\u015ft\u0131rma y\u00f6ntemi, bu deneyde i\u015flemden \u00f6nce ve sonra i\u00e7eri\u011finde k\u00fc\u00e7\u00fck de\u011fi\u015fiklikler olan Cistanche deserticola'daki bile\u015fenlerin tespiti i\u00e7in \u00e7ok uygundur. Metodolojik ara\u015ft\u0131rman\u0131n sonu\u00e7lar\u0131na dayanarak, bu b\u00f6l\u00fcmde olu\u015fturulan y\u00f6ntemin y\u00fcksek hassasiyet, do\u011fruluk ve dayan\u0131kl\u0131l\u0131\u011fa sahip oldu\u011fu bulunabilir. Bu nedenle, bu y\u00f6ntem Cistanche deserticola'n\u0131n i\u015flenmeden \u00f6nce ve sonra bile\u015fik i\u00e7eri\u011finin belirlenmesi i\u00e7in kullan\u0131labilir ve sonu\u00e7lar\u0131 Cistanche deserticola'n\u0131n i\u015flenmeden \u00f6nce ve sonra kalite de\u011ferlendirmesi i\u00e7in kullan\u0131labilir.<br \/>\nCistanche deserticola'n\u0131n i\u015flenmesi s\u0131ras\u0131nda, baz\u0131 bile\u015fikler \u0131s\u0131tman\u0131n etkisiyle hidroliz, oksidasyon, enzimatik ve izomerizasyon reaksiyonlar\u0131na girerek bile\u015fen i\u00e7eriklerinde azalma veya art\u0131\u015fa neden olabilir. E\u011fer verbascoside izoergosterol glikozit \u00fcretmek i\u00e7in izomerize edilirse, verbascoside i\u00e7eri\u011fi azal\u0131rken izoergosterol glikozit i\u00e7eri\u011fi artar. Bununla birlikte, i\u015fleme s\u0131ras\u0131nda, pineal glikozit hidroliz reaksiyonuna yatk\u0131nd\u0131r ve i\u00e7eri\u011finde bir azalmaya yol a\u00e7ar. Bu nedenle, geleneksel \u00c7in t\u0131bb\u0131n\u0131n etkinli\u011fini daha iyi uygulamak i\u00e7in, yetersiz veya a\u015f\u0131r\u0131 i\u015fleme olgusundan ka\u00e7\u0131nmak i\u00e7in i\u015fleme s\u0131ras\u0131nda ana bile\u015fenlerin i\u00e7eri\u011findeki de\u011fi\u015fikliklere dikkat edilmelidir. Genel olarak, bu \u00e7al\u0131\u015fman\u0131n ara\u015ft\u0131rma sonu\u00e7lar\u0131 gelecekte Cistanche deserticola'n\u0131n kalite de\u011ferlendirmesi i\u00e7in teorik bir temel sa\u011flamakta ve Cistanche deserticola'n\u0131n nicel analiz perspektifinden i\u015flenmesinin \u00f6nemini g\u00f6stermektedir.<\/p>","protected":false},"excerpt":{"rendered":"<p>Exploring the effect of processing on the content of five active ingredients in Cistanche tubulosa based on HPLC-QQQ-MS technology. Cistanche tubulosa is a precious Chinese herbal medicine for nourishing the liver and kidneys, nourishing essence and blood, moistening intestines and promoting bowel movements, and delaying aging. It is used to treat kidney deficiency, female infertility, [&hellip;]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.0 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Exploring the effect of processing on the content of five active ingredients in Cistanche deserticola based on HPLC-QQQ-MS technology - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/tr\/cistanche-deserticola\/\" \/>\n<meta property=\"og:locale\" content=\"tr_TR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Exploring the effect of processing on the content of five active ingredients in Cistanche deserticola based on HPLC-QQQ-MS technology - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"Exploring the effect of processing on the content of five active ingredients in Cistanche tubulosa based on HPLC-QQQ-MS technology. 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Cistanche tubulosa is a precious Chinese herbal medicine for nourishing the liver and kidneys, nourishing essence and blood, moistening intestines and promoting bowel movements, and delaying aging. 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