{"id":4624,"date":"2024-08-04T15:03:19","date_gmt":"2024-08-04T15:03:19","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=4624"},"modified":"2024-08-04T15:03:19","modified_gmt":"2024-08-04T15:03:19","slug":"chitin","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/tr\/kitin\/","title":{"rendered":"A\u011fustos b\u00f6ce\u011fi t\u00fcy\u00fcnden kitin ekstraksiyon i\u015fleminin optimizasyonu ve antioksidan aktivitesi ile hemostatik etkisinin de\u011ferlendirilmesi"},"content":{"rendered":"<p>A\u011fustos b\u00f6ce\u011fi t\u00fcy\u00fcnden kitin ekstraksiyon i\u015fleminin optimizasyonu ve antioksidan aktivitesi ile hemostatik etkisinin de\u011ferlendirilmesi<br \/>\nKitosan olarak da bilinen kitin, \u03b2 -1,4-glikosidik ba\u011flarla ba\u011flanm\u0131\u015f N-asetil-2-amino-2-deoksi-D-glukopiranoz birimlerinden olu\u015fan do\u011frusal bir makromolek\u00fcl biyopolisakkarittir. Yar\u0131 \u015feffaf tabaka benzeri bir kat\u0131d\u0131r ve polisakkarit zincirleri aras\u0131ndaki hidrojen ba\u011f\u0131 nedeniyle kitin suda, seyreltik asitlerde, seyreltik bazlarda veya genel organik \u00e7\u00f6z\u00fcc\u00fclerde \u00e7\u00f6z\u00fcnmez. Kitin, hem deniz hem de karasal ortamlarda alt eklembacakl\u0131lar\u0131n, b\u00f6ceklerin kabuklar\u0131nda ve baz\u0131 yosun ve mantarlar\u0131n h\u00fccre duvarlar\u0131nda yayg\u0131n olarak bulunur. Ekstraksiyon y\u00f6ntemleri aras\u0131nda asit-baz, enzimatik hidroliz, EDTA, mikrobiyal fermantasyon ve iyonik s\u0131v\u0131 y\u00f6ntemleri bulunmaktad\u0131r. Kitin antioksidan, hemostatik, antibakteriyel, anti-t\u00fcm\u00f6r, hipoglisemik, hipotansif, lipid d\u00fc\u015f\u00fcr\u00fcc\u00fc ve ba\u011f\u0131\u015f\u0131kl\u0131k d\u00fczenleyici gibi biyolojik aktivitelere sahiptir. Toksik olmamas\u0131, iyi biyouyumlulu\u011fu, biyolojik olarak par\u00e7alanabilirli\u011fi ve enzim bozunma yetene\u011fi ile ila\u00e7 da\u011f\u0131t\u0131m\u0131, doku m\u00fchendisli\u011fi, yara iyile\u015fmesi ve kanser tedavisi gibi biyomedikal alanlarda yayg\u0131n olarak kullan\u0131lmaktad\u0131r.<br \/>\nCicadae olarak da bilinen Periostracum Cicadae, a\u011fustosb\u00f6ce\u011fi familyas\u0131ndan b\u00f6cek siyah \u00e7ekirgenin yumurtadan \u00e7\u0131kt\u0131ktan sonraki deri de\u011fi\u015ftirme kabu\u011fudur. Kimyasal bile\u015fimi temel olarak kitin, protein, inorganik tuzlar, amino asitler, eser elementler vb. i\u00e7erir. \u00c7in'de yayg\u0131n olarak kullan\u0131lan bir geleneksel \u00c7in t\u0131bb\u0131 olarak, r\u00fczgar ve \u0131s\u0131y\u0131 da\u011f\u0131tma, bo\u011faz\u0131 rahatlatma, d\u00f6k\u00fcnt\u00fclere n\u00fcfuz etme, spazmlar\u0131 hafifletme ve g\u00f6r\u00fc\u015f\u00fc iyile\u015ftirme ve opasiteleri hafifletme etkilerine sahiptir. \u00c7al\u0131\u015fmalar, alternatif bir kitin kayna\u011f\u0131 olarak a\u011fustos b\u00f6ce\u011fi t\u00fcy\u00fcn\u00fcn d\u00fc\u015f\u00fck asetilasyon derecesine, kristalli\u011fe, termal \u00f6zelliklere ve iyi ekolojik g\u00fcvenli\u011fe sahip oldu\u011funu g\u00f6stermi\u015ftir. Ancak, a\u011fustos b\u00f6ce\u011fi molt\u00fcn\u00fcn y\u00fcksek fiyat\u0131 ve kitin i\u00e7in sabit kalite standartlar\u0131n\u0131n olmamas\u0131 nedeniyle, a\u011fustos b\u00f6ce\u011fi molt kitini \u00fczerindeki ara\u015ft\u0131rma ilerlemesi \u015fu anda yava\u015ft\u0131r. Bu \u00e7al\u0131\u015fmada, a\u011fustos b\u00f6ce\u011fi t\u00fcylerinden kitin elde etmek i\u00e7in ultrason destekli geleneksel asit-baz y\u00f6ntemi kullan\u0131lm\u0131\u015f, ekstraksiyon d\u00f6ng\u00fcs\u00fc k\u0131salt\u0131lm\u0131\u015f ve tek fakt\u00f6rl\u00fc ve ortogonal deneyler kullan\u0131larak ekstraksiyon s\u00fcreci optimize edilmi\u015ftir. Ekstrakt\u0131n antioksidan aktivitesi ve hemostatik etkisi de belirlenmi\u015ftir ki bu da a\u011fustos b\u00f6ce\u011fi t\u00fcy\u00fc kitininin ara\u015ft\u0131r\u0131lmas\u0131 ve uygulanmas\u0131n\u0131 te\u015fvik etmek i\u00e7in \u00f6nemli bir referans \u00f6neme sahiptir.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4625\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-77.png\" alt=\"\" width=\"774\" height=\"197\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-77.png 774w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-77-768x195.png 768w\" sizes=\"(max-width: 774px) 100vw, 774px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4626\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/2-67.png\" alt=\"\" width=\"775\" height=\"199\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/2-67.png 775w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/2-67-768x197.png 768w\" sizes=\"(max-width: 775px) 100vw, 775px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4627\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/3-63.png\" alt=\"\" width=\"776\" height=\"196\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/3-63.png 776w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/3-63-768x194.png 768w\" sizes=\"(max-width: 776px) 100vw, 776px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4628\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/4-52.png\" alt=\"\" width=\"800\" height=\"655\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/4-52.png 800w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/4-52-768x629.png 768w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4629\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/5-51.png\" alt=\"\" width=\"441\" height=\"575\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4630\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/6-47.png\" alt=\"\" width=\"461\" height=\"275\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4631\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/7-44.png\" alt=\"\" width=\"464\" height=\"301\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4632\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/8-39.png\" alt=\"\" width=\"460\" height=\"282\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4633\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/9-34.png\" alt=\"\" width=\"801\" height=\"577\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/9-34.png 801w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/9-34-768x553.png 768w\" sizes=\"(max-width: 801px) 100vw, 801px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4634\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/10-30.png\" alt=\"\" width=\"801\" height=\"556\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/10-30.png 801w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/10-30-768x533.png 768w\" sizes=\"(max-width: 801px) 100vw, 801px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4635\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/11-27.png\" alt=\"\" width=\"807\" height=\"556\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/11-27.png 807w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/11-27-768x529.png 768w\" sizes=\"(max-width: 807px) 100vw, 807px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4636\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/12-23.png\" alt=\"\" width=\"457\" height=\"256\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4637\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/13-20.png\" alt=\"\" width=\"455\" height=\"256\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4638\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/14-17.png\" alt=\"\" width=\"455\" height=\"260\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4639\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/15-13.png\" alt=\"\" width=\"455\" height=\"669\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4640\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/16-11.png\" alt=\"\" width=\"455\" height=\"304\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/16-11.png 455w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/16-11-325x217.png 325w\" sizes=\"(max-width: 455px) 100vw, 455px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4641\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/17-10.png\" alt=\"\" width=\"454\" height=\"320\" \/><br \/>\nA\u011fustos b\u00f6ce\u011fi kal\u0131plar\u0131ndan kitinin ultrason destekli asit-baz ekstraksiyonu i\u00e7in optimum i\u015flem parametreleri, ortogonal deneylerle birle\u015ftirilmi\u015f tek fakt\u00f6rl\u00fc deneylerle elde edilmi\u015ftir: kire\u00e7 \u00e7\u00f6zme: k\u00fctle fraksiyonu: 8% hidroklorik asit, s\u0131cakl\u0131k: oda s\u0131cakl\u0131\u011f\u0131, kat\u0131-s\u0131v\u0131 oran\u0131: 1:25, ultrason g\u00fcc\u00fc: 160W, ultrason s\u00fcresi: 150 dakika; Protein giderme: k\u00fctle fraksiyonu: 10% sodyum hidroksit, s\u0131cakl\u0131k: 60 \u2103, kat\u0131-s\u0131v\u0131 oran\u0131: 1:25, ultrason g\u00fcc\u00fc: \u00f6nemli bir fark yok, ultrason s\u00fcresi: 90 dakika; Renk giderme: k\u00fctle fraksiyonu: 5% potasyum permanganat, 8% sodyum bis\u00fclfit, s\u0131cakl\u0131k: 90 \u2103, s\u00fcre: 60 dakika boyunca potasyum permanganat reaksiyonu, 90 dakika boyunca sodyum bis\u00fclfit reaksiyonu. Ultrason destekli dekalsifikasyon ve deproteinizasyon, \u00e7\u00f6z\u00fcc\u00fcn\u00fcn penetrasyonunu art\u0131rarak ekstraksiyon s\u00fcresini etkili bir \u015fekilde k\u0131salt\u0131r.<br \/>\n\u00d6n deneyde, potasyum hidroksit\/\u00fcre sulu \u00e7\u00f6zeltisi, a\u011fustos b\u00f6ce\u011fi t\u00fcy\u00fc kitinini \u00e7\u00f6zmek i\u00e7in d\u00fc\u015f\u00fck s\u0131cakl\u0131kta dondurma ve \u00e7\u00f6zme i\u00e7in kullan\u0131lm\u0131\u015ft\u0131r. \u0130\u015flem s\u0131ras\u0131nda, potasyum hidroksit\/\u00fcre sisteminin kendisinin serbest radikaller \u00fczerinde \u00f6nemli bir etkiye sahip oldu\u011fu bulunmu\u015ftur. Bu \u00e7al\u0131\u015fmada a\u011fustos b\u00f6ce\u011fi t\u00fcy\u00fc kitosan s\u00fcspansiyonunun antioksidan aktivitesi ara\u015ft\u0131r\u0131lm\u0131\u015ft\u0131r. Sonu\u00e7lar, a\u011fustos b\u00f6ce\u011fi t\u00fcy\u00fc kitosan s\u00fcspansiyonu konsantrasyonu 18mg\/mL oldu\u011funda, ABTS serbest radikal s\u00fcp\u00fcrme oran\u0131n\u0131n 91 oldu\u011funu g\u00f6stermi\u015ftir.31% oldu\u011funu ve g\u00fc\u00e7l\u00fc serbest radikal s\u00fcp\u00fcrme kabiliyeti g\u00f6sterdi\u011fini; A\u011fustos b\u00f6ce\u011fi t\u00fcy\u00fc kitosan s\u00fcspansiyonu konsantrasyonu 15mg\/mL oldu\u011funda, DPPH serbest radikallerinin s\u00fcp\u00fcrme oran\u0131n\u0131n 35.19% oldu\u011funu; A\u011fustos b\u00f6ce\u011fi t\u00fcy\u00fc kitosan s\u00fcspansiyonu konsantrasyonu 20mg\/mL oldu\u011funda, O2 serbest radikallerinin s\u00fcp\u00fcrme oran\u0131n\u0131n 63.72% oldu\u011funu g\u00f6stermi\u015ftir. Kitosan\u0131n m\u00fckemmel antioksidan aktivite sergilemesinin nedeni, asit-baz ekstraksiyon i\u015flemi s\u0131ras\u0131nda k\u0131smen deasetilasyona u\u011fram\u0131\u015f olmas\u0131 olabilir.<br \/>\nTest t\u00fcp\u00fc y\u00f6ntemi, farkl\u0131 dozlarda kitosan tozu ile tav\u015fan kan\u0131n\u0131n in vitro p\u0131ht\u0131la\u015fma s\u00fcresini belirlemek i\u00e7in kullan\u0131lm\u0131\u015ft\u0131r. Sonu\u00e7lar, cicada molt kitosan tozunun in vitro prokoag\u00fclan yetene\u011finin, cicada molt kitosan dozunun artmas\u0131yla artt\u0131\u011f\u0131n\u0131 g\u00f6stermi\u015ftir. Ayn\u0131 dozdaki Yunnan Baiyao tozu ile kar\u015f\u0131la\u015ft\u0131r\u0131ld\u0131\u011f\u0131nda, a\u011fustos b\u00f6ce\u011fi molt kitosan tozunun in vitro prokoag\u00fclan yetene\u011fi \u00f6nemli \u00f6l\u00e7\u00fcde daha iyiydi. Kuyruk kesme y\u00f6ntemi, fareler \u00fczerinde farkl\u0131 dozlarda cicada molt kitosan tozunun kanama s\u00fcresini ve miktar\u0131n\u0131 belirlemek i\u00e7in kullan\u0131lm\u0131\u015ft\u0131r. Sonu\u00e7lar, her dozda a\u011fustos b\u00f6ce\u011fi erimesi kitosan tozu ve Yunnan Baiyao tozunun pozitif kontrol grubunun, bo\u015f kontrol grubuna k\u0131yasla kanama s\u00fcresini \u00f6nemli \u00f6l\u00e7\u00fcde k\u0131saltabildi\u011fini g\u00f6stermi\u015ftir. Ayn\u0131 dozda Yunnan Baiyao tozu ile kar\u015f\u0131la\u015ft\u0131r\u0131ld\u0131\u011f\u0131nda, kanama s\u00fcresi ve kitosan miktar\u0131nda \u00f6nemli farkl\u0131l\u0131klar vard\u0131, bu da a\u011fustos b\u00f6ce\u011fi molt kitosan\u0131n\u0131n Yunnan Baiyao'dan daha iyi hemostatik yetene\u011fe sahip oldu\u011funu g\u00f6steriyordu. Bir fare karaci\u011fer kanama modeli olu\u015fturarak ve yaran\u0131n kanama s\u00fcresini \u00f6l\u00e7erek, sonu\u00e7lar a\u011fustos b\u00f6ce\u011fi molt kitosan\u0131n\u0131n karaci\u011fer yaras\u0131n\u0131n kanama s\u00fcresini \u00f6nemli \u00f6l\u00e7\u00fcde k\u0131saltabilece\u011fini ve ayn\u0131 dozda a\u011fustos b\u00f6ce\u011fi molt kitosan\u0131n\u0131n verilerinin Yunnan Baiyao pozitif kontrol\u00fcnden daha iyi oldu\u011funu g\u00f6stermi\u015ftir. A\u011fustos b\u00f6ce\u011fi molt kitosan\u0131, karaci\u011fer gibi v\u00fccutta zengin kan i\u00e7eri\u011fine sahip organlar\u0131n kanayan yaralar\u0131nda kan p\u0131ht\u0131la\u015fmas\u0131n\u0131 te\u015fvik etmede iyi bir etkiye sahiptir ve baz\u0131 organlar\u0131n cerrahi rezeksiyonu i\u00e7in hemostatik bir malzeme olarak geli\u015ftirilme potansiyeline sahiptir.<\/p>","protected":false},"excerpt":{"rendered":"<p>Optimization of extraction process of chitin from cicada molt and evaluation of its antioxidant activity and hemostatic effect Chitin, also known as chitosan, is a linear macromolecule biopolysaccharide composed of N-acetyl-2-amino-2-deoxy-D-glucopyranose units connected by \u03b2 -1,4-glycosidic bonds. It is a semi transparent sheet-like solid, and due to the hydrogen bonding between polysaccharide chains, chitin is [&hellip;]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.0 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Optimization of extraction process of chitin from cicada molt and evaluation of its antioxidant activity and hemostatic effect - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/tr\/kitin\/\" \/>\n<meta property=\"og:locale\" content=\"tr_TR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Optimization of extraction process of chitin from cicada molt and evaluation of its antioxidant activity and hemostatic effect - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"Optimization of extraction process of chitin from cicada molt and evaluation of its antioxidant activity and hemostatic effect Chitin, also known as chitosan, is a linear macromolecule biopolysaccharide composed of N-acetyl-2-amino-2-deoxy-D-glucopyranose units connected by \u03b2 -1,4-glycosidic bonds. 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