{"id":4507,"date":"2024-08-04T14:37:37","date_gmt":"2024-08-04T14:37:37","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=4507"},"modified":"2024-08-04T14:37:37","modified_gmt":"2024-08-04T14:37:37","slug":"rhizoma-drynariae","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/tr\/rhizoma-drynariae\/","title":{"rendered":"\u0130nsan osteosarkom h\u00fccresi MG63'te kemik fragman yamas\u0131n\u0131n UPLC parmak izi ile alkalin fosfataz aktivitesi aras\u0131ndaki spektral etki ili\u015fkisi \u00fczerine \u00e7al\u0131\u015fma"},"content":{"rendered":"<p>\u0130nsan osteosarkom h\u00fccresi MG63'te kemik fragman yamas\u0131n\u0131n UPLC parmak izi ile alkalin fosfataz aktivitesi aras\u0131ndaki spektral etki ili\u015fkisi \u00fczerine \u00e7al\u0131\u015fma<br \/>\nGu Duan Bu, bir su ejderi ortopedik bitkisi olan Drynaria fortunei (Kunze) J. Sm.'nin kurutulmu\u015f rizomudur. Do\u011fas\u0131 gere\u011fi s\u0131cak, tad\u0131 ac\u0131d\u0131r ve karaci\u011fer ve b\u00f6brek meridyenlerine aittir. \u0130yile\u015ftirici ve a\u011fr\u0131 giderici, b\u00f6brekleri g\u00fc\u00e7lendirici ve kemikleri g\u00fc\u00e7lendirici, r\u00fczg\u00e2r ve lekeleri giderici etkilere sahiptir. Modern farmakolojik ve klinik \u00e7al\u0131\u015fmalar, kemik par\u00e7as\u0131 toniklerinin osteoporozu \u00f6nleme, k\u0131r\u0131k iyile\u015fmesini destekleme, b\u00f6brekleri koruma, anti-enflamatuar ve di\u015f b\u00fcy\u00fcmesini destekleme gibi etkilere sahip oldu\u011funu g\u00f6stermi\u015ftir. Kimyasal bile\u015fim \u00e7al\u0131\u015fmalar\u0131, kemik k\u0131r\u0131\u011f\u0131 toniklerindeki ana aktif bile\u015fenlerin flavonoidler, triterpenoidler, fenilpropanoidler ve fenoller oldu\u011funu g\u00f6stermi\u015ftir. \u00c7ok say\u0131da \u00e7al\u0131\u015fma, kemik ili\u011fi toniklerinin kemikle ilgili hastal\u0131klar\u0131n tedavisinde \u00f6nemli terap\u00f6tik etkilere sahip oldu\u011funu ve in vitro deneyler kemik ili\u011fi toniklerinin osteoblastlar\u0131n osteojenik farkl\u0131la\u015fma yetene\u011fini destekleyebilece\u011fini g\u00f6stermi\u015ftir. Bununla birlikte, kemik ili\u011fi toniklerindeki spesifik aktif bile\u015fenler hakk\u0131nda herhangi bir rapor bulunmamaktad\u0131r. Alkalin fosfataz (ALP), osteoblastlar\u0131n farkl\u0131la\u015fmas\u0131 ve olgunla\u015fmas\u0131 i\u00e7in bir biyobelirte\u00e7tir ve ALP aktivite seviyelerindeki de\u011fi\u015fiklikler osteoblastlar\u0131n farkl\u0131la\u015fma yetene\u011fini belirlemek i\u00e7in kullan\u0131labilir. Bu nedenle, bu deneyde UPLC parmak izi ile insan osteosarkom MG63 h\u00fccrelerinin ALP aktivite spektrumu aras\u0131ndaki ili\u015fki analiz edilmi\u015f ve osteoblastlar i\u00e7in kullan\u0131lan kemik par\u00e7as\u0131 toniklerinin ana bile\u015fenleri ara\u015ft\u0131r\u0131lm\u0131\u015ft\u0131r.<br \/>\nGeleneksel \u00c7in t\u0131bb\u0131 spektrum etkinli\u011finin de\u011ferlendirme modu, kimyasal bile\u015fen parmak izini farmakolojik etkileriyle birle\u015ftirmektir, b\u00f6ylece parmak izindeki kimyasal bile\u015fenler kar\u015f\u0131l\u0131k gelen farmakolojik etkileri yans\u0131t\u0131r ve parmak izinin karakteristik zirveleri ile farmakolojik etkiler aras\u0131ndaki ili\u015fkiyi netle\u015ftirir, kar\u015f\u0131l\u0131k gelen farmakolojik madde temelini belirler ve b\u00f6ylece geleneksel \u00c7in t\u0131bb\u0131n\u0131n kalite kontrol\u00fcn\u00fc ve farmakolojik de\u011ferlendirmesini ger\u00e7ekle\u015ftirir.<br \/>\nBu \u00e7al\u0131\u015fma, ham ve s\u0131cak kemik par\u00e7as\u0131 takviyelerinin UPLC parmak izi spektrumlar\u0131n\u0131 olu\u015fturmu\u015f ve UPLC-Q-TOF-MS teknolojisini kullanarak kimyasal bile\u015fenlerini tan\u0131mlam\u0131\u015ft\u0131r. Gri korelasyon analizi, iki de\u011fi\u015fkenli korelasyon analizi ve k\u0131smi en k\u00fc\u00e7\u00fck kareler y\u00f6ntemi, kemik par\u00e7as\u0131 takviyelerinin parmak izi spektrumlar\u0131n\u0131 ve ALP aktivitesinin spektral etki ili\u015fkisini olu\u015fturmak i\u00e7in kullan\u0131ld\u0131 ve kemik par\u00e7as\u0131 takviyelerinin farmakolojik madde temelini ve kalite kontrol\u00fcn\u00fc ayd\u0131nlatmak i\u00e7in bir referans sa\u011flad\u0131.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4510\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-68.png\" alt=\"\" width=\"719\" height=\"341\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4511\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/2-58.png\" alt=\"\" width=\"805\" height=\"360\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/2-58.png 805w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/2-58-768x343.png 768w\" sizes=\"(max-width: 805px) 100vw, 805px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4512\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/3-55.png\" alt=\"\" width=\"805\" height=\"172\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/3-55.png 805w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/3-55-768x164.png 768w\" sizes=\"(max-width: 805px) 100vw, 805px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4513\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/4-47.png\" alt=\"\" width=\"805\" height=\"311\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/4-47.png 805w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/4-47-768x297.png 768w\" sizes=\"(max-width: 805px) 100vw, 805px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4514\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/5-46.png\" alt=\"\" width=\"805\" height=\"582\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/5-46.png 805w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/5-46-768x555.png 768w\" sizes=\"(max-width: 805px) 100vw, 805px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4515\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/6-3.jpg\" alt=\"\" width=\"605\" height=\"827\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/6-3.jpg 605w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/6-3-527x720.jpg 527w\" sizes=\"(max-width: 605px) 100vw, 605px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4516\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/7-39.png\" alt=\"\" width=\"606\" height=\"752\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/7-39.png 606w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/7-39-580x720.png 580w\" sizes=\"(max-width: 606px) 100vw, 606px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4517\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/8-34.png\" alt=\"\" width=\"606\" height=\"239\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4518\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/9-29.png\" alt=\"\" width=\"805\" height=\"344\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/9-29.png 805w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/9-29-768x328.png 768w\" sizes=\"(max-width: 805px) 100vw, 805px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4519\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/10-25.png\" alt=\"\" width=\"805\" height=\"435\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/10-25.png 805w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/10-25-768x415.png 768w\" sizes=\"(max-width: 805px) 100vw, 805px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4520\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/11-23.png\" alt=\"\" width=\"805\" height=\"444\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/11-23.png 805w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/11-23-768x424.png 768w\" sizes=\"(max-width: 805px) 100vw, 805px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4521\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/12-19.png\" alt=\"\" width=\"694\" height=\"335\" \/><br \/>\nBu \u00e7al\u0131\u015fmada, kemik par\u00e7as\u0131 takviyeleri ve s\u0131cak \u00fcr\u00fcnlerin parmak izi spektrumlar\u0131n\u0131 olu\u015fturmak i\u00e7in \u00e7oklu dalga boyu tespiti ile birle\u015ftirilmi\u015f UPLC y\u00f6ntemi kullan\u0131lm\u0131\u015ft\u0131r. Kemik par\u00e7as\u0131 takviyeleri ve s\u0131cak \u00fcr\u00fcnlerin farkl\u0131 partileri aras\u0131nda 12 ortak pik vard\u0131 ve benzerlik 0,9'dan b\u00fcy\u00fckt\u00fc, bu da ikisi aras\u0131nda y\u00fcksek benzerlik ve belirsiz farkl\u0131la\u015fma oldu\u011funu g\u00f6steriyordu. ALP, osteoblast farkl\u0131la\u015fmas\u0131 ve olgunla\u015fmas\u0131n\u0131n bir biyobelirtecidir ve kemik olu\u015fumunun biyokimyasal g\u00f6stergelerinden biridir. Osteoblastlar\u0131n farkl\u0131la\u015fma yetene\u011fi ALP aktivite seviyelerindeki de\u011fi\u015fikliklerle belirlenebilir. \u00d6nceki \u00e7al\u0131\u015fmalar kemik par\u00e7as\u0131 takviyesinin osteoblast farkl\u0131la\u015fmas\u0131n\u0131 destekleyebilece\u011fini g\u00f6stermi\u015ftir. Bu \u00e7al\u0131\u015fmada, kemik par\u00e7as\u0131 takviyeleri ve ha\u015flamalar, ALP aktivitesi \u00fczerindeki etkilerini analiz etmek i\u00e7in MG63 h\u00fccrelerine uygulanm\u0131\u015ft\u0131r. Sonu\u00e7lar, ayn\u0131 kemik par\u00e7as\u0131 grubunun kum ha\u015flamas\u0131ndan sonra MG63 h\u00fccreleri taraf\u0131ndan ALP salg\u0131lanmas\u0131n\u0131 \u00f6nemli \u00f6l\u00e7\u00fcde te\u015fvik edebilece\u011fini g\u00f6stermektedir. MG63 osteojenik farkl\u0131la\u015fmas\u0131n\u0131 te\u015fvik eden kemik par\u00e7alanmas\u0131 ve onar\u0131m\u0131ndaki aktif bile\u015fenleri ara\u015ft\u0131rmak i\u00e7in, ALP aktivitesi ile parmak izi spektrumlar\u0131 ile kalibre edilen karakteristik pikler aras\u0131nda spektral bir korelasyon analizi yap\u0131lm\u0131\u015ft\u0131r. Kemik par\u00e7as\u0131 yama parmak izi spektrumunun karakteristik pikleri ile ALP aktivitesi aras\u0131ndaki spektral etki ili\u015fkisini kapsaml\u0131 bir \u015fekilde analiz etmek i\u00e7in bu deneyde \u00fc\u00e7 y\u00f6ntem kullan\u0131lm\u0131\u015ft\u0131r: gri korelasyon analizi, iki de\u011fi\u015fkenli korelasyon analizi ve en k\u00fc\u00e7\u00fck kareler regresyon analizi. Pik 4, pik 5 ve pik 12'nin ALP aktivitesi ile y\u00fcksek korelasyona sahip oldu\u011fu ve pozitif ili\u015fkili oldu\u011fu, ALP aktivitesine en fazla katk\u0131da bulundu\u011fu ve ALP aktivitesini etkileyen \u00f6nemli de\u011fi\u015fkenler oldu\u011fu bulunmu\u015ftur.<br \/>\nUPLC-Q-TOF\/MS teknolojisi, kemik par\u00e7alar\u0131n\u0131n kimyasal bile\u015fenlerini daha kapsaml\u0131 ve h\u0131zl\u0131 bir \u015fekilde tan\u0131mlayabilen g\u00fc\u00e7l\u00fc se\u00e7icilik, y\u00fcksek hassasiyet, basit numune \u00f6n i\u015flemi, y\u00fcksek do\u011fruluk ve y\u00fcksek \u00e7\u00f6z\u00fcn\u00fcrl\u00fck \u00f6zelliklerine sahiptir. Bu \u00e7al\u0131\u015fmada, kemik par\u00e7as\u0131 toniklerindeki 40 bile\u015feni tan\u0131mlamak i\u00e7in negatif iyon modunda UPLC-Q-TOF\/MS kullan\u0131lm\u0131\u015ft\u0131r. ALP aktivitesine \u00f6nemli \u00f6l\u00e7\u00fcde katk\u0131da bulunan tan\u0131mlanm\u0131\u015f bile\u015fen pik 4 kafeik asit-4-O - \u03b2 - D-glukopiranozit; Pik 5 kate\u015finler 7-glukozit; Pik 12 naringin ve aktivitesi do\u011frulanm\u0131\u015ft\u0131r. Naringin flavonoid bir bile\u015fiktir ve modern farmakolojik ara\u015ft\u0131rmalar naringinin osteoporoz, antioksidan, anti-t\u00fcm\u00f6r ve miyokardiyal hasar\u0131n iyile\u015ftirilmesi gibi \u00e7e\u015fitli hastal\u0131klar\u0131 tedavi etmek veya \u00f6nlemek i\u00e7in kullan\u0131labilece\u011fini g\u00f6stermi\u015ftir. Mevcut ara\u015ft\u0131rmalar ayr\u0131ca naringinin kemik par\u00e7as\u0131 toniklerinde \u00f6nemli bir aktif bile\u015fen oldu\u011funu g\u00f6stermektedir. Kafeik asit-4-O - \u03b2 - D-glukopiranosid ve kate\u015fin 7-glukosidin farmakolojik aktiviteleri hen\u00fcz rapor edilmemi\u015ftir ve daha fazla ara\u015ft\u0131rma yap\u0131lmas\u0131 gerekmektedir. Bu \u00e7al\u0131\u015fman\u0131n sonu\u00e7lar\u0131, kemik par\u00e7as\u0131 toniklerinin kalite kontrol\u00fc ve farmakolojik ara\u015ft\u0131rmalar\u0131 i\u00e7in belirli referans \u00f6nemine sahiptir.<\/p>","protected":false},"excerpt":{"rendered":"<p>Study on the spectral effect relationship between UPLC fingerprint of bone fragment patch and alkaline phosphatase activity in human osteosarcoma cell MG63 Gu Duan Bu is the dried rhizome of Drynaria fortunei (Kunze) J. Sm., a water dragon orthopedic plant. It is warm in nature, bitter in taste, and belongs to the liver and kidney [&hellip;]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.0 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Study on the spectral effect relationship between UPLC fingerprint of bone fragment patch and alkaline phosphatase activity in human osteosarcoma cell MG63 - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/tr\/rhizoma-drynariae\/\" \/>\n<meta property=\"og:locale\" content=\"tr_TR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Study on the spectral effect relationship between UPLC fingerprint of bone fragment patch and alkaline phosphatase activity in human osteosarcoma cell MG63 - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"Study on the spectral effect relationship between UPLC fingerprint of bone fragment patch and alkaline phosphatase activity in human osteosarcoma cell MG63 Gu Duan Bu is the dried rhizome of Drynaria fortunei (Kunze) J. 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Sm., a water dragon orthopedic plant. 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