{"id":4459,"date":"2024-08-04T14:28:35","date_gmt":"2024-08-04T14:28:35","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=4459"},"modified":"2024-08-04T14:28:35","modified_gmt":"2024-08-04T14:28:35","slug":"natural-low-melting-point-solvent","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/tr\/dogal-dusuk-erime-noktali-cozucu\/","title":{"rendered":"Oleuropeinin sel\u00fclaz hidrolizinin do\u011fal \u00f6tektik \u00e7\u00f6z\u00fcc\u00fcler taraf\u0131ndan te\u015fvik edilmesi \u00fczerine \u00e7al\u0131\u015fma"},"content":{"rendered":"<p>Oleuropeinin sel\u00fclaz hidrolizinin do\u011fal \u00f6tektik \u00e7\u00f6z\u00fcc\u00fcler taraf\u0131ndan te\u015fvik edilmesi \u00fczerine \u00e7al\u0131\u015fma<br \/>\nOlea europaea L., Oleaceae familyas\u0131na ve Oleaceae cinsine ait bir bitkidir. Akdeniz b\u00f6lgesinde \u00f6nemli bir ya\u011f bitkisidir ve Gansu ve Yunnan \u00c7in'de zeytin i\u00e7in ana ekim alanlar\u0131d\u0131r. Zeytin yapraklar\u0131, zeytin a\u011fac\u0131 yeti\u015ftiricili\u011finin ucuz bir yan \u00fcr\u00fcn\u00fcd\u00fcr. Her zeytin a\u011fac\u0131n\u0131n her y\u0131l yakla\u015f\u0131k 25 kg yaprak kesmesi gerekir ve hasat edilen yapraklar\u0131n toplam a\u011f\u0131rl\u0131\u011f\u0131 zeytinin toplam a\u011f\u0131rl\u0131\u011f\u0131n\u0131n 10%'sini olu\u015fturur. Zeytin yapraklar\u0131 \u00e7e\u015fitli biyoaktif bile\u015fikler i\u00e7erir. \u00d6rne\u011fin oleanolik asit, al\u0131\u00e7 asidi, ursolik asit, oleuropein, hidroksitirosol vb.<br \/>\nAra\u015ft\u0131rmalar, oleuropeinin zeytin a\u011fa\u00e7lar\u0131n\u0131n t\u00fcm k\u0131s\u0131mlar\u0131na da\u011f\u0131ld\u0131\u011f\u0131n\u0131 ve en y\u00fcksek i\u00e7eri\u011fin zeytin yapraklar\u0131nda bulundu\u011funu g\u00f6stermi\u015ftir. Kuru zeytin yapraklar\u0131nda oleuropein i\u00e7eri\u011fi 10% ila 17%'ye ula\u015fabilir. \u015eekil 1'de g\u00f6sterildi\u011fi gibi, oleuropein hidrolize olarak hidroksitirosol, glikoz ve olenik asit \u00fcretir. Bunlar aras\u0131nda hidroksitirosol, oleuropeinden daha iyi biyolojik aktivite sergiler. \u015eu anda, oleuropeinin hidroliz y\u00f6ntemleri temel olarak asit hidrolizi, alkali hidroliz ve enzimatik hidrolizi i\u00e7ermektedir. Asit hidrolizi ve alkalin hidrolizi, kat\u0131 reaksiyon ko\u015fullar\u0131na sahip g\u00fc\u00e7l\u00fc asitler, g\u00fc\u00e7l\u00fc bazlar ve y\u00fcksek s\u0131cakl\u0131k ortamlar\u0131 gerektirir. Son y\u0131llarda, yan \u00fcr\u00fcnlerin biyotransformasyonu i\u00e7in kataliz\u00f6r olarak enzimlerin kullan\u0131m\u0131 dikkat \u00e7ekmektedir. Geleneksel asit-baz hidroliz y\u00f6ntemleriyle kar\u015f\u0131la\u015ft\u0131r\u0131ld\u0131\u011f\u0131nda, oleuropeinin enzimatik hidrolizi, hafif reaksiyon ko\u015fullar\u0131 ve daha az \u00e7evre kirlili\u011fi avantajlar\u0131na sahiptir. Liu ve arkada\u015flar\u0131, glikozidik ba\u011flar\u0131 k\u0131rmak ve oleuropeini hidrolize ederek hidroksitirosol elde etmek i\u00e7in bir g\u0131da katk\u0131 maddesi olarak sel\u00fclaz kullanm\u0131\u015ft\u0131r. Sel\u00fclaz\u0131n termal karars\u0131zl\u0131\u011f\u0131 nedeniyle, enzimin optimum s\u0131cakl\u0131\u011f\u0131nda s\u00fcrekli olarak y\u00fcksek aktiviteyi koruyamaz, bu da d\u00fc\u015f\u00fck enzimatik hidroliz verimlili\u011fine neden olur. Bu nedenle, oleuropeinin \u00e7\u00f6z\u00fcn\u00fcrl\u00fc\u011f\u00fcn\u00fc art\u0131rabilen ve uzun s\u00fcreli reaksiyonlarda sel\u00fclaz\u0131n stabilitesini art\u0131rabilen ve b\u00f6ylece oleuropeinin enzimatik hidroliz verimlili\u011fini art\u0131rabilen tampon \u00e7\u00f6zeltinin yerini alacak yeni bir ye\u015fil \u00e7\u00f6z\u00fcc\u00fc bulmaya \u00e7al\u0131\u015f\u0131yoruz.<br \/>\nDerin \u00f6tektik \u00e7\u00f6z\u00fcc\u00fcler (DES), hidrojen ba\u011f\u0131 al\u0131c\u0131lar\u0131 (HBA) ve hidrojen ba\u011f\u0131 don\u00f6rlerinin (HBD) y\u00fcksek s\u0131cakl\u0131kta kar\u0131\u015ft\u0131r\u0131lmas\u0131yla olu\u015fan, tek tek bile\u015fenlere k\u0131yasla daha d\u00fc\u015f\u00fck erime noktalar\u0131na sahip homojen ve \u015feffaf kar\u0131\u015f\u0131mlard\u0131r. D\u00fc\u015f\u00fck yan\u0131c\u0131l\u0131k, d\u00fc\u015f\u00fck u\u00e7uculuk, kolay haz\u0131rlama, y\u00fcksek \u00e7\u00f6z\u00fcn\u00fcrl\u00fck ve enzimlerle y\u00fcksek uyumluluk gibi m\u00fckemmel fiziksel ve kimyasal \u00f6zellikleri nedeniyle DES, toksik olmayan bir ekstraktant ve \u00e7\u00f6z\u00fcn\u00fcrle\u015ftirici olarak giderek daha fazla kullan\u0131lmaktad\u0131r. En \u00f6nemli \u015fey, enzimlerin organik \u00e7\u00f6z\u00fcc\u00fclerde deaktivasyona e\u011filimli olmas\u0131 ve DES'in bir \u00e7\u00f6z\u00fcc\u00fc olarak bu fenomenin meydana gelmesini \u00f6nleyebilmesidir. Bu ayn\u0131 zamanda DES'in organik \u00e7\u00f6z\u00fcc\u00fclerin yerini almas\u0131n\u0131 ve sel\u00fclazlar\u0131 ink\u00fcbe etmesini m\u00fcmk\u00fcn k\u0131lar. Do\u011fal HBA ve HBD'den olu\u015fan DES, geleneksel DES'ten daha g\u00fcvenli ve daha \u00e7evreci olan NADES olarak adland\u0131r\u0131l\u0131r. NADES, enzim katalizli reaksiyonlar alan\u0131nda ye\u015fil bir \u00e7\u00f6z\u00fcc\u00fc olarak yayg\u0131n ilgi g\u00f6rm\u00fc\u015ft\u00fcr. NADES, belirli enzimatik reaksiyonlarda ba\u015far\u0131yla uygulanm\u0131\u015f ve hem enzim aktivitesi hem de stabilitesi \u00fczerinde olumlu etkiler yaratm\u0131\u015ft\u0131r. Yukar\u0131daki ara\u015ft\u0131rma sonu\u00e7lar\u0131, NADES'in oleuropeinin sel\u00fclaz hidrolizi i\u00e7in iyi bir \u00e7\u00f6z\u00fcc\u00fc olma potansiyeline sahip oldu\u011funu g\u00f6stermektedir.<br \/>\nBu \u00e7al\u0131\u015fmada, HBA olarak kolin klor\u00fcr veya betain ve HBD olarak farkl\u0131 poliol t\u00fcrleri kullan\u0131larak alt\u0131 tip NADES haz\u0131rlanm\u0131\u015f ve bunlar\u0131n oleuropeinin sel\u00fclaz hidrolizi \u00fczerindeki etkileri ara\u015ft\u0131r\u0131lm\u0131\u015ft\u0131r. Farkl\u0131 NADES t\u00fcrlerinin ve konsantrasyonlar\u0131n\u0131n ve bunlar\u0131n monomerlerinin enzimatik hidroliz h\u0131z\u0131 \u00fczerindeki etkileri ara\u015ft\u0131r\u0131larak, oleuropeinin sel\u00fclaz hidrolizi i\u00e7in yeni, verimli ve ye\u015fil bir y\u00f6ntem olu\u015fturulmu\u015ftur.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4462\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-64.png\" alt=\"\" width=\"669\" height=\"179\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4463\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/2-54.png\" alt=\"\" width=\"673\" height=\"199\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4464\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/3-52.png\" alt=\"\" width=\"342\" height=\"343\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/3-52.png 342w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/3-52-200x200.png 200w\" sizes=\"(max-width: 342px) 100vw, 342px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4465\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/4-44.png\" alt=\"\" width=\"344\" height=\"239\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4466\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/5-43.png\" alt=\"\" width=\"672\" height=\"239\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4467\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/6-39.png\" alt=\"\" width=\"671\" height=\"329\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4468\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/7-37.png\" alt=\"\" width=\"685\" height=\"334\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4469\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/8-32.png\" alt=\"\" width=\"679\" height=\"506\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4470\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/9-2.jpg\" alt=\"\" width=\"651\" height=\"236\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4471\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/10-23.png\" alt=\"\" width=\"674\" height=\"234\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4472\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/11-21.png\" alt=\"\" width=\"680\" height=\"237\" \/>Bu \u00e7al\u0131\u015fmada, tampon \u00e7\u00f6zeltiye farkl\u0131 tip ve konsantrasyonlarda NADES eklenerek NADES'in oleuropeinaz\u0131n hidroliz h\u0131z\u0131 \u00fczerindeki etkisi ara\u015ft\u0131r\u0131lm\u0131\u015ft\u0131r. Uygun NADES t\u00fcr\u00fc ve konsantrasyonunun se\u00e7ilmesinin sel\u00fclaz\u0131n stabilitesini art\u0131rabilece\u011fi ve oleuropeinaz\u0131n hidrolizini te\u015fvik edebilece\u011fi bulunmu\u015ftur. 50\u00b0C s\u0131cakl\u0131k ve pH=5 ko\u015fullar\u0131 alt\u0131nda, 10% DES-5 (V\/V) \u00e7\u00f6z\u00fcc\u00fc sisteminde oleuropeinaz\u0131n hidroliz oran\u0131, 3 saat hidrolizden sonra tampon \u00e7\u00f6zeltinin 1,7 kat\u0131yd\u0131; 36 saatlik hidrolizden sonra enzimatik hidroliz oran\u0131 tampon \u00e7\u00f6zeltinin 1,5 kat\u0131d\u0131r; Hafif, verimli ve \u00e7evre dostu enzimatik hidroliz ko\u015fullar\u0131 temelinde, bu y\u00f6ntem sel\u00fclaz\u0131n oleuropeini uzun s\u00fcre verimli bir \u015fekilde hidrolize etmesini m\u00fcmk\u00fcn k\u0131lar. Bu \u00e7al\u0131\u015fmada, sel\u00fclaz\u0131n stabilitesini art\u0131rmak ve oleuropeinaz\u0131n hidrolizini te\u015fvik etmek i\u00e7in NADES'te HBA olarak kolin klor\u00fcr veya betain ve HBD olarak polioller kullan\u0131lm\u0131\u015f ve oleuropeinaz hidroliz \u00fcr\u00fcnlerinin verimli bir \u015fekilde haz\u0131rlanmas\u0131 i\u00e7in bilimsel bir temel sa\u011flanm\u0131\u015ft\u0131r.<\/p>","protected":false},"excerpt":{"rendered":"<p>Study on the promotion of cellulase hydrolysis of oleuropein by natural eutectic solvents Olea europaea L. is a plant belonging to the Oleaceae family and the Oleaceae genus. It is an important oil crop in the Mediterranean region, and Gansu and Yunnan are the main planting areas for olives in China. Olive leaves are a [&hellip;]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.0 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Study on the promotion of cellulase hydrolysis of oleuropein by natural eutectic solvents - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/tr\/dogal-dusuk-erime-noktali-cozucu\/\" \/>\n<meta property=\"og:locale\" content=\"tr_TR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Study on the promotion of cellulase hydrolysis of oleuropein by natural eutectic solvents - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"Study on the promotion of cellulase hydrolysis of oleuropein by natural eutectic solvents Olea europaea L. is a plant belonging to the Oleaceae family and the Oleaceae genus. It is an important oil crop in the Mediterranean region, and Gansu and Yunnan are the main planting areas for olives in China. 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It is an important oil crop in the Mediterranean region, and Gansu and Yunnan are the main planting areas for olives in China. 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