{"id":4246,"date":"2024-08-04T08:58:38","date_gmt":"2024-08-04T08:58:38","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=4246"},"modified":"2024-08-04T08:58:38","modified_gmt":"2024-08-04T08:58:38","slug":"chinese-rose","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/tr\/chinese-rose\/","title":{"rendered":"G\u00fcl standart kaynatmas\u0131ndaki \u00e7oklu bile\u015fenlerin UPLC parmak izi ve kantitatif analizi"},"content":{"rendered":"<p>G\u00fcl standart kaynatmas\u0131ndaki \u00e7oklu bile\u015fenlerin UPLC parmak izi ve kantitatif analizi<br \/>\nG\u00fcl k\u0131rm\u0131z\u0131s\u0131, Changchun k\u0131rm\u0131z\u0131s\u0131 vb. olarak da bilinen g\u00fcl \u00e7i\u00e7ekleri, Rosaceae familyas\u0131ndan Rosa chinensis Jacq. t\u00fcr\u00fcn\u00fcn kurutulmu\u015f \u00e7i\u00e7ekleridir. \u00c7in'in \u00e7e\u015fitli b\u00f6lgelerinde ekilirler ve y\u0131l boyunca hasat edilebilirler. \u00c7i\u00e7ekler hafif a\u00e7\u0131kken toplan\u0131r ve g\u00f6lgede ya da d\u00fc\u015f\u00fck s\u0131cakl\u0131klarda kurutulur. G\u00fcl \u00e7i\u00e7eklerinin t\u0131bbi de\u011feri ilk olarak Li Shizhen'in \"Compendium of Materia Medica\" adl\u0131 eserinde kaydedilmi\u015ftir. Tad\u0131 tatl\u0131d\u0131r, do\u011fas\u0131 s\u0131cakt\u0131r ve karaci\u011fer meridyenine aittir; kan dola\u015f\u0131m\u0131n\u0131 te\u015fvik etme, menstr\u00fcasyonu d\u00fczenleme ve depresyonu hafifletmek i\u00e7in karaci\u011feri yat\u0131\u015ft\u0131rma etkilerine sahiptir. Esas olarak qi durgunlu\u011fu ve kan staz\u0131, adet bozukluklar\u0131, g\u00f6\u011f\u00fcs ve kaburga \u015fi\u015fkinli\u011fi ve a\u011fr\u0131s\u0131 gibi \u00e7e\u015fitli hastal\u0131klar\u0131n tedavisinde kullan\u0131l\u0131r. G\u00fcl \u00e7i\u00e7ekleri flavonoidler, fenolik asitler, aromatik ya\u011flar, tanenler ve pigmentler dahil olmak \u00fczere \u00e7e\u015fitli kimyasal bile\u015fenler i\u00e7erir; bunlar aras\u0131nda flavonoidler ve fenolik asitler g\u00fcl \u00e7i\u00e7eklerinin ana t\u0131bbi bile\u015fenleri olarak kabul edilir. Flavonoidler, kuersetin, kuersetin, kaempferol-3-O-rhamnosid, kaempferol, s\u00fcksinik asit, gallik asit, etil gallat, hiperosid, hesperidin, izoquercitrin vb. dahil olmak \u00fczere ana bile\u015fenlerdir. Antiviral, antioksidan, ba\u011f\u0131\u015f\u0131kl\u0131k g\u00fc\u00e7lendirici ve antikanser etkileri gibi biyolojik aktivitelere sahiptirler.<br \/>\nGeleneksel \u00c7in t\u0131bb\u0131n\u0131n bile\u015fimi karma\u015f\u0131kt\u0131r ve farmakolojik etkileri, v\u00fccut \u00fczerinde d\u00fczenleyici etkiler uygulayan \u00e7oklu hedefler, yollar ve genel sistemler \u00fczerindeki \u00e7oklu aktif bile\u015fenlerin sinerjik etkilerine dayanmaktad\u0131r. Geleneksel \u00c7in t\u0131bb\u0131n\u0131n parmak izi, geleneksel \u00c7in t\u0131bb\u0131n\u0131n kimyasal bile\u015fenlerinin sistematik olarak incelenmesine dayanmaktad\u0131r. Geleneksel \u00c7in t\u0131bb\u0131 bile\u015fenlerinin bilgilerini madde gruplar\u0131 perspektifinden kapsaml\u0131 bir \u015fekilde g\u00f6sterir ve geleneksel \u00c7in t\u0131bb\u0131 kimyasal bile\u015fenlerinin genel durumunu yans\u0131t\u0131r. Kapsaml\u0131, \u00f6l\u00e7\u00fclebilir ve etkili bir teknik ara\u00e7t\u0131r. Bu \u00e7al\u0131\u015fma, kimyasal \u00f6r\u00fcnt\u00fc tan\u0131ma teknolojisi ile birle\u015ftirilmi\u015f UPLC y\u00f6ntemini kullanarak 20 parti g\u00fcl \u00e7i\u00e7e\u011fi standart kaynatmas\u0131n\u0131n parmak izi spektrumlar\u0131n\u0131 olu\u015fturmu\u015f, g\u00fcl \u00e7i\u00e7e\u011fi standart kaynatmas\u0131n\u0131 etkileyen ana karakteristik bile\u015fenleri analiz etmi\u015f ve ara\u015ft\u0131rm\u0131\u015f ve g\u00fcl \u00e7i\u00e7e\u011fi standart kaynatmas\u0131yla ilgili preparatlar\u0131n ve kalite standard\u0131 ara\u015ft\u0131rmalar\u0131n\u0131n geli\u015ftirilmesi i\u00e7in referans sa\u011flamak amac\u0131yla \u00e7oklu g\u00f6sterge bile\u015fenlerinin nicel analizini ger\u00e7ekle\u015ftirmi\u015ftir.<\/p>\n<p>&nbsp;<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4247\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-42.png\" alt=\"\" width=\"619\" height=\"185\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4248\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/2-40.png\" alt=\"\" width=\"804\" height=\"539\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/2-40.png 804w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/2-40-325x217.png 325w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/2-40-768x515.png 768w\" sizes=\"(max-width: 804px) 100vw, 804px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4249\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/3-39.png\" alt=\"\" width=\"804\" height=\"745\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/3-39.png 804w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/3-39-768x712.png 768w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/3-39-777x720.png 777w\" sizes=\"(max-width: 804px) 100vw, 804px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4250\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/4-35.png\" alt=\"\" width=\"804\" height=\"537\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/4-35.png 804w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/4-35-650x433.png 650w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/4-35-325x217.png 325w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/4-35-768x513.png 768w\" sizes=\"(max-width: 804px) 100vw, 804px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4251\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/5-34.png\" alt=\"\" width=\"804\" height=\"325\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/5-34.png 804w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/5-34-768x310.png 768w\" sizes=\"(max-width: 804px) 100vw, 804px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4252\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/6-34.png\" alt=\"\" width=\"804\" height=\"409\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/6-34.png 804w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/6-34-768x391.png 768w\" sizes=\"(max-width: 804px) 100vw, 804px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4253\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/7-31.png\" alt=\"\" width=\"804\" height=\"341\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/7-31.png 804w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/7-31-768x326.png 768w\" sizes=\"(max-width: 804px) 100vw, 804px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4254\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/8-27.png\" alt=\"\" width=\"804\" height=\"692\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/8-27.png 804w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/8-27-768x661.png 768w\" sizes=\"(max-width: 804px) 100vw, 804px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4255\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/9-22.png\" alt=\"\" width=\"804\" height=\"342\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/9-22.png 804w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/9-22-768x327.png 768w\" sizes=\"(max-width: 804px) 100vw, 804px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4256\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/10-18.png\" alt=\"\" width=\"804\" height=\"305\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/10-18.png 804w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/10-18-768x291.png 768w\" sizes=\"(max-width: 804px) 100vw, 804px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4257\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/11.jpg\" alt=\"\" width=\"804\" height=\"649\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/11.jpg 804w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/11-768x620.jpg 768w\" sizes=\"(max-width: 804px) 100vw, 804px\" \/>Standart kaynatman\u0131n temsil kabiliyetini sa\u011flamak i\u00e7in, bu \u00e7al\u0131\u015fmada g\u00fcl \u00e7i\u00e7e\u011fi hammaddelerinin ana \u00fcretim alanlar\u0131 hakk\u0131nda bir ara\u015ft\u0131rma yap\u0131lm\u0131\u015ft\u0131r. Hebei Eyaletindeki Baoding \u015eehri, Henan Eyaletindeki Zhoukou \u015eehri, Shandong Eyaletindeki Heze \u015eehri, Shandong Eyaletindeki Linyi \u015eehri ve Yunnan Eyaletindeki Dali \u0130li gibi b\u00fcy\u00fck ve otantik \u00fcretim alanlar\u0131ndan yerinde numuneler de dahil olmak \u00fczere toplam 20 parti numune toplanm\u0131\u015ft\u0131r. \"Geleneksel \u00c7in T\u0131bb\u0131 Form\u00fcl Gran\u00fcllerinin Kalite Kontrol\u00fc ve Standart Geli\u015ftirilmesi i\u00e7in Teknik Gereklilikler\" k\u0131lavuz ilkelerine g\u00f6re ve \"T\u0131bbi Kurumlarda Geleneksel \u00c7in T\u0131bb\u0131 Kaynatma Odas\u0131 i\u00e7in Y\u00f6netim \u015eartnamesi\" referans al\u0131narak, standart kaynatma \u00f6n i\u015flem y\u00f6ntemleri (kaynatma s\u00fcreleri, su ilavesi, kaynatma s\u00fcresi ve di\u011fer g\u00f6stergeler ve parametreler) en uygun haz\u0131rlama s\u00fcrecini belirlemek i\u00e7in ara\u015ft\u0131r\u0131l\u0131r; D\u00fc\u015f\u00fck s\u0131cakl\u0131kta konsantrasyon ve dondurarak kurutma kullan\u0131larak, standart bir g\u00fcl kaynatma dondurularak kurutulmu\u015f toz yap\u0131l\u0131r.<br \/>\nParmak izi y\u00f6nteminin olu\u015fturulmas\u0131 s\u00fcrecinde, ekstraksiyon \u00e7\u00f6z\u00fcc\u00fclerinin (metanol, 70% metanol, 50% metanol, etanol, 70% etanol, seyreltik etanol), ekstraksiyon y\u00f6ntemlerinin (ultrason, refl\u00fc) ve ekstraksiyon s\u00fcrelerinin (10, 30, 45, 60 dakika) parmak izi \u00fczerindeki etkileri ara\u015ft\u0131r\u0131lm\u0131\u015ft\u0131r. Ekstraksiyonun yeterlili\u011fi, kararl\u0131l\u0131\u011f\u0131 ve kullan\u0131m kolayl\u0131\u011f\u0131 g\u00f6z \u00f6n\u00fcnde bulundurularak sonu\u00e7ta 30 dakika s\u00fcreyle 50% metanol ultrasonik i\u015flem se\u00e7ilmi\u015ftir. Standart g\u00fcl \u00e7orbas\u0131 \u00f6rnekleri \u00fczerinde tam dalga boyu taramas\u0131 (200-400nm) ger\u00e7ekle\u015ftirmek i\u00e7in bir diyot dizisi dedekt\u00f6r\u00fc kullan\u0131lm\u0131\u015f ve farkl\u0131 dalga boylar\u0131ndaki kromatogramlar\u0131n pik say\u0131lar\u0131, yan\u0131t de\u011ferleri ve \u00e7\u00f6z\u00fcn\u00fcrl\u00fckleri kar\u015f\u0131la\u015ft\u0131r\u0131lm\u0131\u015ft\u0131r. Sonu\u00e7lar, 254nm'nin tespit dalga boyu olarak kullan\u0131lmas\u0131n\u0131n daha fazla say\u0131da kromatografik pik ve daha b\u00fcy\u00fck yan\u0131t de\u011ferleri, kararl\u0131 bir taban \u00e7izgisi ve daha az giri\u015fim ile sonu\u00e7land\u0131\u011f\u0131n\u0131 g\u00f6stermi\u015ftir. Ayn\u0131 zamanda, mobil faz su faz\u0131, kolon s\u0131cakl\u0131\u011f\u0131 ve ak\u0131\u015f h\u0131z\u0131n\u0131n kromatografik ko\u015fullar\u0131 ara\u015ft\u0131r\u0131lm\u0131\u015f ve sonu\u00e7 olarak 0,4 mL\/dak ak\u0131\u015f h\u0131z\u0131 ve 35 \u2103 kolon s\u0131cakl\u0131\u011f\u0131 ile mobil faz su faz\u0131 olarak 0,2% fosforik asit \u00e7\u00f6zeltisi kullan\u0131lmas\u0131na karar verilmi\u015ftir.<br \/>\nFarkl\u0131 k\u00f6kenlerden gelen g\u00fcl \u00e7i\u00e7eklerinin standart kayna\u011f\u0131n\u0131 analiz etmek ve kar\u015f\u0131la\u015ft\u0131rmak i\u00e7in kimyasal \u00f6r\u00fcnt\u00fc tan\u0131ma teknolojisi ile birle\u015ftirilmi\u015f parmak izi spektrumu kullan\u0131lm\u0131\u015ft\u0131r. Sonu\u00e7lar, denetimsiz HCA ve PCA analizinin farkl\u0131 k\u00f6kenlerden gelen \u00f6rneklerin d\u00f6rt kategoride k\u00fcmelenebilece\u011fini ortaya koydu\u011funu g\u00f6stermi\u015ftir. OPLS-DA analizi, ba\u015fta flavonoidler ve fenolik asit bile\u015fenleri olmak \u00fczere, farkl\u0131 k\u00f6kenlerden gelen g\u00fcl standart kaynatmalar\u0131n\u0131n ay\u0131rt edilmesinde \u00f6nemli bir rol oynayan toplam 11 farkl\u0131 biyobelirte\u00e7 tan\u0131mlam\u0131\u015ft\u0131r. Bunlar, g\u00fcl standart dekoksiyonlar\u0131 i\u00e7in kalite kontrol g\u00f6stergeleri olarak hizmet edebilir ve g\u00fcl form\u00fcl\u00fc gran\u00fcllerinin hammadde kaynaklar\u0131 i\u00e7in daha kapsaml\u0131 bir referans sa\u011flayabilir.<br \/>\nBu \u00e7al\u0131\u015fmada, 20 parti g\u00fcl standart kaynatma \u00fcr\u00fcn\u00fcn\u00fcn kalitesi \u00fczerine bir \u00f6n \u00e7al\u0131\u015fma yapmak i\u00e7in k\u00fcmeleme, temel bile\u015fen analizi, ortogonal k\u0131smi en k\u00fc\u00e7\u00fck kareler diskriminant analizi ve \u00e7oklu indeks bile\u015fen i\u00e7eri\u011fi belirleme ile birlikte parmak izi spektrumlar\u0131 kullan\u0131lm\u0131\u015ft\u0131r. Bu y\u00f6ntem, farkl\u0131 t\u0131bbi malzeme gruplar\u0131n\u0131n kalitesini etkileyen \u00f6nemli kromatografik pikleri h\u0131zl\u0131 bir \u015fekilde tarayabilir. Bunlar aras\u0131nda gallik asit, tannik asit, hiperosid, izoquercitrin, astragalosid, quercitrin ve quercetin y\u00fcksek \u00e7\u00f6z\u00fcn\u00fcrl\u00fckl\u00fc ve referans madde spektral kar\u015f\u0131la\u015ft\u0131rmas\u0131 yoluyla tan\u0131mlanm\u0131\u015ft\u0131r. Sonu\u00e7lar, y\u00fcksek fenolik asit ve flavonoid i\u00e7eri\u011fine sahip \u00f6rneklerin Henan Eyaletinden topland\u0131\u011f\u0131n\u0131 ve HCA ve PCA analizinde S11-S13 kategorilerine ait oldu\u011funu g\u00f6stermi\u015ftir. En d\u00fc\u015f\u00fck i\u00e7eri\u011fe sahip \u00f6rnekler ise Yunnan Eyaletinden toplanm\u0131\u015f olup ilk kategori olan S1-S6'ya aittir. Bu, sonraki g\u00fcl standart kaynatma i\u00e7in hammadde koleksiyonunun k\u00f6kenini se\u00e7mek i\u00e7in bir temel sa\u011flayabilir. \u015eu anda, ara\u015ft\u0131rmalar g\u00fcl \u00e7i\u00e7eklerinin esas olarak antioksidan, antiviral, antifungal, antikanser, anti-t\u00fcm\u00f6r ve anti-aging gibi biyolojik aktivitelere sahip flavonoidler, flavonoid glikozitler ve fenolik asit bile\u015fikleri i\u00e7erdi\u011fini g\u00f6stermektedir. Daha sonraki a\u015famada, g\u00fcl \u00e7i\u00e7e\u011fi standart dekoksiyonlar\u0131n\u0131n ve ilgili preparatlar\u0131n kalite kontrol\u00fc i\u00e7in referans sa\u011flamak \u00fczere ila\u00e7 etkinli\u011fine dayal\u0131 derinlemesine ara\u015ft\u0131rmalar yap\u0131lacakt\u0131r.<\/p>","protected":false},"excerpt":{"rendered":"<p>UPLC fingerprint and quantitative analysis of multiple components in rose standard decoction Rose flowers, also known as rose red, Changchun red, etc., are the dried flowers of Rosa chinensis Jacq. in the Rosaceae family. They are planted in various regions of China and can be harvested throughout the year. They are picked when the flowers [&hellip;]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.0 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>UPLC fingerprint and quantitative analysis of multiple components in rose standard decoction - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/tr\/chinese-rose\/\" \/>\n<meta property=\"og:locale\" content=\"tr_TR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"UPLC fingerprint and quantitative analysis of multiple components in rose standard decoction - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"UPLC fingerprint and quantitative analysis of multiple components in rose standard decoction Rose flowers, also known as rose red, Changchun red, etc., are the dried flowers of Rosa chinensis Jacq. in the Rosaceae family. They are planted in various regions of China and can be harvested throughout the year. 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