{"id":4179,"date":"2024-08-04T08:48:04","date_gmt":"2024-08-04T08:48:04","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=4179"},"modified":"2024-08-04T08:48:04","modified_gmt":"2024-08-04T08:48:04","slug":"qianhu-medicinal-materials","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/tr\/qianhu-tibbi\u0307-malzemeler\/","title":{"rendered":"Houttuynia cordata t\u0131bbi malzemelerinin kalitesinin kimyasal \u00f6r\u00fcnt\u00fc tan\u0131ma ve \u00e7oklu indeks bile\u015feni kantitatif de\u011ferlendirmesi ile birle\u015ftirilmi\u015f HPLC parmak izine dayal\u0131"},"content":{"rendered":"

Houttuynia cordata t\u0131bbi malzemelerinin kalitesinin kimyasal \u00f6r\u00fcnt\u00fc tan\u0131ma ve \u00e7oklu indeks bile\u015feni kantitatif de\u011ferlendirmesi ile birle\u015ftirilmi\u015f HPLC parmak izine dayal\u0131
\nPeucedamum praeruptorum Dunn \u015fifal\u0131 otu, Umbelliferae familyas\u0131ndan beyaz \u00e7i\u00e7ekli Peucedamum praeruptorum Dunn bitkisinin kurutulmu\u015f k\u00f6klerinden elde edilir. \u0130lk olarak \"\u00dcnl\u00fc Doktorlar\u0131n Kay\u0131tlar\u0131 \"nda kaydedilmi\u015ftir ve r\u00fczgar \u0131s\u0131s\u0131n\u0131 da\u011f\u0131tma, qi'yi azaltma ve balgam\u0131 \u00e7\u00f6zme etkilerine sahiptir. Balgam \u0131s\u0131s\u0131 \u00f6ks\u00fcr\u00fc\u011f\u00fc ve ast\u0131m, sar\u0131 kal\u0131n balgam, r\u00fczgar \u0131s\u0131s\u0131 \u00f6ks\u00fcr\u00fc\u011f\u00fc ve a\u015f\u0131r\u0131 balgam gibi semptomlar i\u00e7in yayg\u0131n olarak kullan\u0131l\u0131r. Modern kimyasal bile\u015fim ve klinik uygulamalar a\u00e7\u0131s\u0131ndan, Peucedanum praeruptorum'un aktif bile\u015fenleri esas olarak balgam s\u00f6kt\u00fcr\u00fcc\u00fc, kalp yetmezli\u011fi \u00f6nleyici, kan bas\u0131nc\u0131n\u0131 d\u00fc\u015f\u00fcr\u00fcc\u00fc ve anti-enflamatuar gibi farmakolojik etkilere sahip Peucedanum praeruptorum A, Peucedanum praeruptorum B, Peucedanum praeruptorum Ding vb. dahil olmak \u00fczere boynuz tipi kumarinlerdir. Eski zamanlardan beri, Qianhu'nun \u015fifal\u0131 bitkisi \u00e7o\u011funlukla Anhui, Hunan, Jiangxi, Zhejiang, Jiangsu ve di\u011fer yerlerde da\u011f\u0131t\u0131lan yabani kaynaklar olmu\u015ftur. Son y\u0131llarda, kaynak talebindeki art\u0131\u015f nedeniyle, yapay ekim yava\u015f yava\u015f yabani ekimin yerini alm\u0131\u015ft\u0131r. Ba\u015fl\u0131ca ekim alanlar\u0131 Anhui'deki Ningguo, Hunan'daki Huarong, Guizhou'daki Zunyi, Chongqing'deki Fengjie ve di\u011fer yerlerdir. Beyaz \u00e7i\u00e7ekli Peucedanum praeruptorum'un yapay olarak yeti\u015ftirilmesinden sonra, do\u011fuda Zhejiang'dan bat\u0131da Yunnan ve Sichuan'a kadar \u00e7e\u015fitli \u00fcretim alanlar\u0131 bu bitkiyi tan\u0131tm\u0131\u015f ve ekmi\u015ftir. Bununla birlikte, \u00fcretim ortamlar\u0131ndaki b\u00fcy\u00fck farkl\u0131l\u0131klar nedeniyle, Peucedanum praeruptorum t\u0131bbi malzemelerinin kalitesinde, de\u011fi\u015fen kalite seviyeleriyle birlikte \u00f6nemli farkl\u0131l\u0131klar vard\u0131r. Buna ek olarak, beyaz \u00e7i\u00e7ekli Peucedanum praeruptorum'un yapay ekimi, Peucedanum praeruptorum t\u0131bbi malzemelerinin erken hasad\u0131n\u0131 zorlayan erken bolting gibi sorunlara e\u011filimlidir. Yetersiz b\u00fcy\u00fcme d\u00f6nemi, t\u0131bbi malzemelerin niteliksiz oran\u0131nda \u00f6nemli bir art\u0131\u015fa yol a\u00e7maktad\u0131r. \u00c7in Farmakopesi'nin 2020 bask\u0131s\u0131, \"Peucedanum praeruptorum A i\u00e7eri\u011finin 0,90%'den az olmamas\u0131n\u0131 ve Peucedanum praeruptorum B i\u00e7eri\u011finin 0,24%'den az olmamas\u0131n\u0131\" \u015fart ko\u015fmaktad\u0131r. Ancak, piyasadaki Peucedanum praeruptorum t\u0131bbi malzemelerinin kalite uygunluk oran\u0131 sadece 26%'dir ve kalifiye olmayan Peucedanum praeruptorum t\u0131bbi malzemeleri piyasay\u0131 doldurmaktad\u0131r. Y\u00fcksek kaliteli yeni \u00e7e\u015fitlerin yeti\u015ftirilmesi, uygun ekim tekniklerinin olu\u015fturulmas\u0131 ve \u00fcretim alanlar\u0131nda ilk i\u015flemlerin standartla\u015ft\u0131r\u0131lmas\u0131n\u0131n yan\u0131 s\u0131ra, objektif, bilimsel ve makul kalite de\u011ferlendirme y\u00f6ntemleri ve standartlar\u0131 da Qianhu t\u0131bbi malzemelerinin kalitesini sa\u011flaman\u0131n \u00f6nemli y\u00f6nleridir. \u015eu anda, Houttuynia cordata t\u0131bbi malzemelerinin kalitesini de\u011ferlendirmenin anahtar\u0131, beyaz \u00e7i\u00e7ekli Houttuynia cordata'daki berberin ve berberin i\u00e7eri\u011finde yatmaktad\u0131r. Ancak, bu genellikle Houttuynia cordata t\u0131bbi malzemelerinin i\u00e7sel kalitesini kapsaml\u0131 bir \u015fekilde de\u011ferlendiremez ve geleneksel \u00c7in t\u0131bb\u0131n\u0131n klinik uygulamas\u0131n\u0131 do\u011frudan etkiler.<\/p>\n

HPLC parmak izi, t\u0131bbi malzemelerin i\u00e7sel kalitesini kapsaml\u0131 bir \u015fekilde yans\u0131tabilir ve yayg\u0131n olarak tan\u0131nan bir kalite kontrol y\u00f6ntemidir. \u00c7oklu indeks i\u00e7erik belirleme ile birle\u015ftirildi\u011finde, geleneksel \u00c7in t\u0131bbi malzemeleri i\u00e7in kalite kontrol\u00fcn ana ak\u0131m\u0131 haline gelmi\u015ftir. Hiyerar\u015fik k\u00fcmeleme analizi (HCA) ve temel bile\u015fen analizi (PCA) gibi denetimsiz y\u00f6ntemlerin yan\u0131 s\u0131ra k\u0131smi en k\u00fc\u00e7\u00fck kareler (PLS) ve yapay sinir a\u011flar\u0131 gibi denetimli kimyasal modelleme y\u00f6ntemleri, t\u0131bbi malzemelerin i\u00e7sel kalitesini daha iyi yans\u0131tabilir ve son y\u0131llarda yayg\u0131n olarak kullan\u0131lan yeni y\u00f6ntemlerdir. Bu \u00e7al\u0131\u015fma, Houttuynia cordata'n\u0131n farkl\u0131 k\u00f6kenlerinden gelen t\u0131bbi malzemeleri kapsaml\u0131 bir \u015fekilde de\u011ferlendirmek i\u00e7in bir HPLC parmak izi olu\u015fturarak ve k\u00fcme analizi, temel bile\u015fen analizi ve k\u0131smi en k\u00fc\u00e7\u00fck kareler regresyon analizi gibi kimyasal modelleme y\u00f6ntemlerini birle\u015ftirerek \u00f6nceki temele dayanmaktad\u0131r; Ayn\u0131 zamanda, bergamot lakton, paeoniflorin, paeoniflorin ve paeoniflorin E olmak \u00fczere d\u00f6rt kumarin bile\u015feninin i\u00e7eri\u011fi, farkl\u0131 b\u00f6lgelerden gelen paeoniflorinin bile\u015fimsel farkl\u0131l\u0131klar\u0131n\u0131 a\u00e7\u0131kl\u0131\u011fa kavu\u015fturmak ve paeoniflorin i\u00e7in kalite standartlar\u0131n\u0131n geli\u015ftirilmesi i\u00e7in referans sa\u011flamak \u00fczere kantitatif olarak belirlenmi\u015ftir.<\/p>\n

\"\"\"\"
\nHouttuynia cordata'n\u0131n HPLC parmak izinin kromatografik ko\u015fullar\u0131n\u0131 optimize etmek i\u00e7in, bu \u00e7al\u0131\u015fma \u00f6nceki ara\u015ft\u0131rmalara dayanarak tespit dalga boyu, mobil faz ve ak\u0131\u015f h\u0131z\u0131n\u0131 optimize etmi\u015ftir. Analiz tam dalga boyu tarama y\u00f6ntemi kullan\u0131larak ger\u00e7ekle\u015ftirilmi\u015f ve sonu\u00e7lar 190-245nm ve 290-340nm dalga boylar\u0131nda absorpsiyon g\u00f6stermi\u015ftir. Tespit dalga boyu olarak 321nm'nin se\u00e7ilmesi b\u00fcy\u00fck miktarda bilgiye, iyi yan\u0131t de\u011ferine ve safs\u0131zl\u0131klardan daha az giri\u015fime sahiptir. Bu nedenle 321nm tespit dalga boyu olarak belirlenmi\u015ftir. \"Asetonitril su\" ve \"metanol su\" mobil fazlar\u0131n\u0131n incelenmesi, \"metanol su \"nun ay\u0131rma etkisinin daha iyi oldu\u011funu ve 0.5% formik asit suyun daha iyi pik \u015fekilleri elde edebilece\u011fini g\u00f6stermi\u015ftir. Bu nedenle, mobil faz olarak \"metanol-0.5% formik asit suyu\" belirlenmi\u015ftir. Ayr\u0131ca, ak\u0131\u015f \u00fczerinde de incelemeler yap\u0131lm\u0131\u015ft\u0131r \"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"1, 0,8 ve 0,5 mL\/dak ak\u0131\u015f h\u0131zlar\u0131nda \u00e7al\u0131\u015f\u0131lm\u0131\u015f ve 0,5 mL\/dak'n\u0131n daha iyi ay\u0131rma verimlili\u011fine sahip oldu\u011fu g\u00f6r\u00fclm\u00fc\u015ft\u00fcr. Bu nedenle, 0,5 mL\/dak bu y\u00f6ntem i\u00e7in ak\u0131\u015f h\u0131z\u0131 olarak belirlenmi\u015ftir.<\/p>\n

Bu \u00e7al\u0131\u015fmada Hunan, Anhui, Chongqing, Hubei, Guizhou, Yunnan ve di\u011fer \u00fcretim alanlar\u0131ndan \u00f6rnekleri analiz etmek i\u00e7in HPLC parmak izi kullan\u0131lm\u0131\u015ft\u0131r. \u00c7in \u015fifal\u0131 bitkilerinden olu\u015fan 16 partinin parmak izleri y\u00fcksek benzerlik g\u00f6stermi\u015f, toplam 21 ortak pik ve 7 bile\u015fen tan\u0131mlanm\u0131\u015ft\u0131r. Kimyasal desen analizinde, 16 parti numune \u00fc\u00e7 kategoriye ayr\u0131lm\u0131\u015ft\u0131r. Referans literat\u00fcr raporlar\u0131na dayanarak, bunlar \u00fc\u00e7 kategoriye ayr\u0131labilir: Anhui'deki Ningguo ve Bozhou gibi otantik \u00fcretim alanlar\u0131, \u00e7o\u011funlukla Hunan'daki Qianhu, Guizhou, Chongqing ve Yunnan'daki y\u00fcksek rak\u0131ml\u0131 \u00fcretim alanlar\u0131. Temel bile\u015fen ve PLS-DA analizi yoluyla, alt\u0131 temel bile\u015fenin k\u00fcm\u00fclatif varyans katk\u0131 oran\u0131 86.213% olmu\u015ftur. Daha ileri analizler, men\u015fei ay\u0131rt eden ve men\u015fein ana karakteristik bile\u015fenleri olarak kullan\u0131labilecek sekiz ortak tepe noktas\u0131 belirlemi\u015ftir. Y\u00fcksek rak\u0131ml\u0131 Yunnan \u00fcretim alanlar\u0131ndan al\u0131nan Houttuynia cordata \u00f6rneklerindeki berberin ve bergamot i\u00e7eri\u011fi di\u011fer \u00fcretim alanlar\u0131na g\u00f6re \u00f6nemli \u00f6l\u00e7\u00fcde daha y\u00fcksekken, berberin di\u011fer \u00fcretim alanlar\u0131na g\u00f6re biraz daha y\u00fcksektir. Y\u00fcksek rak\u0131m\u0131n Houttuynia cordata t\u0131bbi malzemelerinde bergamot lakton, berberin ve berberin birikimine elveri\u015fli oldu\u011funu tahmin ediyoruz. Yang ve arkada\u015flar\u0131 y\u00fcksek rak\u0131m\u0131n Peucedanum praeruptorum ve Peucedanum praeruptorum'un olu\u015fumu ve birikimi i\u00e7in elveri\u015fli oldu\u011funa inan\u0131rken, Luo ve arkada\u015flar\u0131 rak\u0131m\u0131n Peucedanum praeruptorum'un i\u00e7eri\u011fi \u00fczerinde Peucedanum praeruptorum'dan daha b\u00fcy\u00fck bir etkiye sahip oldu\u011funa inanmaktad\u0131r. Bu \u00e7al\u0131\u015fma \u00f6nceki ara\u015ft\u0131rmalardan farkl\u0131 olsa da, y\u00fcksek rak\u0131ml\u0131 ortamlar\u0131n Houttuynia cordata t\u0131bbi malzemelerinin kalitesinin olu\u015fumuna daha elveri\u015fli oldu\u011fu a\u00e7\u0131kl\u0131\u011fa kavu\u015fturulabilir.<\/p>\n

\u00c7oklu indeks bile\u015fen i\u00e7eri\u011fi belirleme analizinde, toplam 9 parti Peucedanum praeruptorum \u00f6rne\u011fi, 56.25% ile Peucedanum praeruptorum A i\u00e7in \u00c7in Farmakopesi gerekliliklerini kar\u015f\u0131lam\u0131\u015ft\u0131r. Bununla birlikte, hi\u00e7bir parti Peucedanum praeruptorum B i\u00e7in limit gerekliliklerini kar\u015f\u0131lamam\u0131\u015ft\u0131r ve en y\u00fcksek de\u011fer sadece 2,01 mg\/g'd\u0131r. Xu vd. ve Liu vd. ayr\u0131ca Houttuynia cordata t\u0131bbi bitkisinde, beyaz \u00e7i\u00e7ekli Houttuynia cordata A'n\u0131n standard\u0131 kar\u015f\u0131lama olas\u0131l\u0131\u011f\u0131n\u0131n daha y\u00fcksek oldu\u011funu, beyaz \u00e7i\u00e7ekli Houttuynia cordata B'nin standard\u0131 kar\u015f\u0131lamama olgusunun ise daha yayg\u0131n oldu\u011funu tespit etmi\u015ftir. Beyaz \u00e7i\u00e7ekli Houttuynia cordata bir kez \u00e7i\u00e7ek a\u00e7an \u00e7ok y\u0131ll\u0131k bir bitkidir. B\u00fcy\u00fck \u00f6l\u00e7ekli ekim, y\u0131ll\u0131k bir \u00e7e\u015fide d\u00f6n\u00fc\u015fmesine yol a\u00e7m\u0131\u015ft\u0131r ve yetersiz ya\u015f, Houttuynia cordata t\u0131bbi malzemelerinin kalitesindeki d\u00fc\u015f\u00fc\u015f\u00fcn \u00f6nemli bir nedenidir. Buna ek olarak, beyaz \u00e7i\u00e7ekli Houttuynia cordata erken boltinge e\u011filimlidir ve \"barut tohumlar\u0131\" (y\u0131ll\u0131k tohumlar) piyasay\u0131 doldurmaktad\u0131r. Uzun s\u00fcreli yinelemeli seleksiyon yoluyla, Houttuynia cordata t\u0131bbi malzemelerinin kalitesi yapay olarak se\u00e7ici bir \u015fekilde y\u0131ldan y\u0131la d\u00fc\u015fmektedir. Qianhu \u015fifal\u0131 bitkilerinin kalitesindeki yayg\u0131n d\u00fc\u015f\u00fc\u015f\u00fcn klinik etkinli\u011fi etkileyip etkilemedi\u011fini belirlemek i\u00e7in daha fazla ara\u015ft\u0131rmaya ihtiya\u00e7 vard\u0131r.<\/p>\n

Literat\u00fcr raporlar\u0131na g\u00f6re, Peucedanum praeruptorum A ve Peucedanum praeruptorum B i\u00e7eri\u011fi aras\u0131nda belirli bir derecede negatif korelasyon vard\u0131r. Bu \u00e7al\u0131\u015fma da bu e\u011filimi bulmu\u015ftur, ancak belirli nedenlerin daha fazla do\u011frulanmas\u0131 gerekmektedir. Piyasadaki \u00c7in \u015fifal\u0131 bitkilerinin kalitesizli\u011finin nedenleri, ekolojik \u00e7evre, genetik fakt\u00f6rler ve yapay m\u00fcdahalenin yan\u0131 s\u0131ra, mevcut kalite de\u011ferlendirmesiyle de yak\u0131ndan ilgili olabilir. Mevcut standartlarda, piyasadaki sat\u0131\u015f \u00f6ncesi Hu t\u0131bbi malzemelerinin yeterlilik oran\u0131, \u00f6zellikle de Qianhu t\u0131bbi malzemelerinin klinik kullan\u0131m\u0131 ve end\u00fcstriyel geli\u015fimi ile art\u0131k uyumlu olmayan Baihua Qianhu Yi'nin i\u00e7eri\u011finin uygunluk oran\u0131 son derece d\u00fc\u015f\u00fckt\u00fcr. Bu ba\u011flamda, Qiu ve arkada\u015flar\u0131 Peucedanum praeruptorum t\u0131bbi malzemelerinin kalitesini kapsaml\u0131 bir \u015fekilde de\u011ferlendirmek i\u00e7in Peucedanum praeruptorum A ve Peucedanum praeruptorum B oran\u0131n\u0131n veya toplam\u0131n\u0131n kullan\u0131m\u0131n\u0131 ara\u015ft\u0131rm\u0131\u015ft\u0131r. Liu ve di\u011ferleri, Xiao ve di\u011ferleri, Shi ve di\u011ferleri Peucedanum praeruptorum t\u0131bbi malzemelerinin parmak izi spektrumu \u00fczerinde farkl\u0131 a\u00e7\u0131lardan de\u011ferlendirme \u00e7al\u0131\u015fmalar\u0131 yapm\u0131\u015f ve iyi beklentiler elde etmi\u015flerdir.<\/p>\n

Bu \u00e7al\u0131\u015fmada t\u0131bbi malzemelerin kalitesini analiz etmek ve de\u011ferlendirmek i\u00e7in parmak izi analizi, \u00e7oklu indeks bile\u015fen analizi ve kimyasal model analizinin bir kombinasyonu kullan\u0131lm\u0131\u015ft\u0131r; bu da Qianhu t\u0131bbi malzemeleri i\u00e7in kalite de\u011ferlendirme standartlar\u0131n\u0131n form\u00fcle edilmesinde \u00f6nemli bir referans niteli\u011fi ta\u015f\u0131maktad\u0131r. Bununla birlikte, bu \u00e7al\u0131\u015fmada toplanan \u00f6rneklerin \u00e7o\u011funlukla pazarlardan, dikim \u00fcslerinden ve di\u011fer kaynaklardan elde edilmi\u015f olmas\u0131, germplazm, ekolojik \u00e7evre, dikim teknikleri ve di\u011fer bilgilerin ayr\u0131nt\u0131l\u0131 kay\u0131tlar\u0131n\u0131n bulunmamas\u0131 bir eksikliktir. \u00d6rnek tutarl\u0131l\u0131\u011f\u0131n\u0131n s\u0131n\u0131rland\u0131r\u0131lmas\u0131n\u0131n bu \u00e7al\u0131\u015fma \u00fczerinde baz\u0131 etkileri olabilir. Sonraki ara\u015ft\u0131rmalarda, ekolojik \u00e7evrenin Houttuynia cordata'n\u0131n kalitesi \u00fczerindeki etkisinin incelenmesini g\u00fc\u00e7lendirmek, beyaz \u00e7i\u00e7ekli Houttuynia cordata'n\u0131n erken boltinginin temel sorununu ele almak, kalite standard\u0131 ara\u015ft\u0131rmas\u0131n\u0131n \u00f6rneklem boyutunu art\u0131rmak ve pazar beklentilerini kar\u015f\u0131layan ve end\u00fcstrinin s\u00fcrd\u00fcr\u00fclebilir geli\u015fimini te\u015fvik eden kalite de\u011ferlendirme standartlar\u0131 geli\u015ftirmek i\u00e7in \u00e7aba g\u00f6sterilmelidir.<\/p>","protected":false},"excerpt":{"rendered":"

Houttuynia cordata t\u0131bbi malzemelerinin kalitesinin kimyasal \u00f6r\u00fcnt\u00fc tan\u0131ma ve \u00e7oklu indeks bile\u015feni kantitatif de\u011ferlendirmesi ile birlikte HPLC parmak izine dayanarak Peucedamum praeruptorum Dunn \u015fifal\u0131 otu, Umbelliferae familyas\u0131ndaki beyaz \u00e7i\u00e7ekli Peucedamum praeruptorum Dunn bitkisinin kurutulmu\u015f k\u00f6klerinden elde edilir. \u0130lk olarak \"Record [...]'da kaydedilmi\u015ftir.<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"\nBased on HPLC fingerprint combined with chemical pattern recognition and multi index component quantitative evaluation of the quality of Houttuynia cordata medicinal materials - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/tr\/qianhu-tibbi\u0307-malzemeler\/\" \/>\n<meta property=\"og:locale\" content=\"tr_TR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Based on HPLC fingerprint combined with chemical pattern recognition and multi index component quantitative evaluation of the quality of Houttuynia cordata medicinal materials - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"Based on HPLC fingerprint combined with chemical pattern recognition and multi index component quantitative evaluation of the quality of Houttuynia cordata medicinal materials The medicinal herb of Peucedamum praeruptorum Dunn is derived from the dried roots of the white flowered Peucedamum praeruptorum Dunn plant in the Umbelliferae family. 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