{"id":3512,"date":"2024-08-02T14:18:37","date_gmt":"2024-08-02T14:18:37","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=3512"},"modified":"2024-08-02T14:18:37","modified_gmt":"2024-08-02T14:18:37","slug":"chamagu-isothiocyanate","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/tr\/samagu-i\u0307zoti\u0307osi\u0307yanat\/","title":{"rendered":"Chamagu izotiyosiyanat\u0131n enzimatik haz\u0131rlanmas\u0131 ve anti servikal kanser aktivitesi"},"content":{"rendered":"

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Chamagu izotiyosiyanat\u0131n enzimatik haz\u0131rlanmas\u0131 ve anti servikal kanser aktivitesi<\/p>\n

Son y\u0131llarda \u00c7in'de kanser g\u00f6r\u00fclme oran\u0131 h\u0131zla artmaktad\u0131r ve kanserin \u00f6nlenmesi ve tedavisi acildir. Ara\u015ft\u0131rmalar, turpgil sebzelerin al\u0131m\u0131n\u0131n art\u0131r\u0131lmas\u0131n\u0131n kanser g\u00f6r\u00fclme oran\u0131n\u0131 etkili bir \u015fekilde azaltabilece\u011fini g\u00f6stermektedir. Brassicaceae sebzeleri, glukozinolatlar (GL'ler) olarak da bilinen bol miktarda glukozinolat i\u00e7erir. Bitkiler mekanik olarak zarar g\u00f6rd\u00fc\u011f\u00fcnde, GL'ler kendi myrosinazlar\u0131 taraf\u0131ndan hidrolize edilerek izotiyosiyanatlar \u00fcretilebilir. \u0130zotiyosiyanatlar, R-N=C=S molek\u00fcler form\u00fcl\u00fcne sahip konjuge \u00e7ift ba\u011f i\u00e7eren bir bile\u015fik s\u0131n\u0131f\u0131d\u0131r (R, farkl\u0131 alkil gruplar\u0131n\u0131 temsil eder). Son y\u0131llarda izotiyosiyanatlar, ola\u011fan\u00fcst\u00fc biyolojik aktiviteleri nedeniyle ara\u015ft\u0131rmalar\u0131n odak noktas\u0131 haline gelmi\u015ftir. Ara\u015ft\u0131rmalar, izotiyosiyanatlar\u0131n anti-t\u00fcm\u00f6r, antibakteriyel, serbest radikal s\u00fcp\u00fcrme ve patojen direnci gibi \u00e7e\u015fitli biyolojik aktivitelere sahip oldu\u011funu g\u00f6stermi\u015ftir. Sa\u011fl\u0131k, g\u0131da ve tar\u0131m gibi bir\u00e7ok alanda geli\u015ftirilmi\u015f ve kullan\u0131lm\u0131\u015ft\u0131r.<\/p>\n

Bilimsel ad\u0131 \"\u015falgam\" olan Brassica rapa L., Brassicaceae familyas\u0131na ait iki y\u0131ll\u0131k otsu bir bitkidir. Esas olarak Sincan'daki Tianshan Da\u011flar\u0131'n\u0131n g\u00fcneybat\u0131s\u0131nda ve Tarim'in kuzeybat\u0131s\u0131nda da\u011f\u0131l\u0131m g\u00f6sterir ve Sincan'daki t\u00fcm etnik gruplardan insanlar taraf\u0131ndan yayg\u0131n olarak t\u00fcketilen yerel bir \u00f6zel sebzedir. Ara\u015ft\u0131rmalar Chamagu k\u00f6klerinin bol miktarda GL i\u00e7erdi\u011fini ortaya koymu\u015ftur. Zhuang ve arkada\u015flar\u0131, Sincan \u015falgam\u0131n\u0131n farkl\u0131 geli\u015fim a\u015famalar\u0131nda ve b\u00fcy\u00fcme d\u00f6nemlerinde (k\u00f6kler, g\u00f6vdeler, yapraklar) GL'lerin t\u00fcrlerini ve i\u00e7eriklerini analiz etmek i\u00e7in ultra y\u00fcksek s\u0131v\u0131 kromatografi teknolojisini kullanm\u0131\u015ft\u0131r. Besinsel b\u00fcy\u00fcmenin farkl\u0131 geli\u015fim a\u015famalar\u0131nda glukozinolatlar\u0131n t\u00fcrleri ve k\u00fctle fraksiyonlar\u0131nda belirli farkl\u0131l\u0131klar oldu\u011funu bulmu\u015flard\u0131r. Bitkilerdeki GL'lerin t\u00fcrleri ve i\u00e7eriklerindeki farkl\u0131l\u0131klar, izotiyosiyanatlar\u0131n t\u00fcrleri ve i\u00e7eriklerinde de\u011fi\u015fikliklere yol a\u00e7arak farkl\u0131 biyolojik aktivitelere neden olacakt\u0131r. Sun, Qiamagu tohumlar\u0131n\u0131n d\u00f6rt tip izotiyosiyanat i\u00e7erdi\u011fini bildirmi\u015ftir: fenetil izotiyosiyanat, tert butil izotiyosiyanat, izopropil izotiyosiyanat ve allil izotiyosiyanat.<\/p>\n

\u015eu anda, izotiyosiyanatlar\u0131n haz\u0131rlanmas\u0131 esas olarak kimyasal sentez ve enzimatik hidrolizi i\u00e7ermektedir. Bunlar aras\u0131nda kimyasal sentez y\u00f6ntemi karma\u015f\u0131k ad\u0131mlara, bir\u00e7ok ara \u00fcr\u00fcne sahiptir, \u00e7evre dostu de\u011fildir ve d\u00fc\u015f\u00fck sentez oran\u0131na sahiptir; Enzimatik hidroliz, basit i\u015flem ve nispeten y\u00fcksek verim avantajlar\u0131na sahip olan izotiyosiyanatlar\u0131 \u00e7\u0131karmak i\u00e7in turpgillerdeki glukozinolat mirosinaz sistemini kullan\u0131r. Enzimatik hidroliz, endojen mirosinaz bozunma y\u00f6ntemi ve eksojen mirosinaz bozunma y\u00f6ntemi olarak ikiye ayr\u0131l\u0131r. \u0130zotiyosiyanatlar\u0131 haz\u0131rlamak i\u00e7in endojen enzimatik hidrolizi kullanmak, i\u015flem basit olmas\u0131na ra\u011fmen, bitkilerdeki endojen mirosinaz tamamen serbest b\u0131rak\u0131lamaz, bu da uzun enzimatik hidroliz s\u00fcresine ve d\u00fc\u015f\u00fck verime neden olur. Ding, kolza tohumunda endojen ve eksojen enzimler taraf\u0131ndan GL'lerin par\u00e7alanmas\u0131yla \u00fcretilen izotiyosiyanat miktar\u0131n\u0131 kar\u015f\u0131la\u015ft\u0131rm\u0131\u015f ve eksojen mirosinaz taraf\u0131ndan \u00fcretilen izotiyosiyanat miktar\u0131n\u0131n endojen mirosinaz\u0131n 7,6 kat\u0131 oldu\u011funu bulmu\u015ftur. GC-MS tan\u0131mlama ve analizi, eksojen mirosinaz\u0131n \u00e7e\u015fitli bozunma \u00fcr\u00fcnlerine, nispeten y\u00fcksek i\u00e7eri\u011fe ve daha az safs\u0131zl\u0131\u011fa sahip oldu\u011funu g\u00f6stermi\u015ftir.<\/p>\n

Bu \u00e7al\u0131\u015fmada hammadde olarak Chamagu k\u00f6k\u00fc kullan\u0131lm\u0131\u015f ve Chamagu izotiyosiyanat (BRICe) haz\u0131rlamak i\u00e7in eksojen mirosinaz par\u00e7alama y\u00f6ntemi kullan\u0131lm\u0131\u015ft\u0131r. Tek fakt\u00f6rl\u00fc tarama temelinde, enzim hidroliz s\u00fcresi, s\u0131cakl\u0131k, pH ve askorbik asit konsantrasyonu \u00f6nemli etki eden fakt\u00f6rler olarak belirlenmi\u015ftir. Ekstraksiyon s\u00fcrecini optimize etmek i\u00e7in d\u00f6rt fakt\u00f6rl\u00fc \u00fc\u00e7 seviyeli ortogonal deney kullan\u0131lm\u0131\u015ft\u0131r. Son olarak, kimyasal bile\u015fimi gaz kromatografisi-k\u00fctle spektrometresi (GC-MS) ile analiz edilmi\u015f ve izotiyosiyanat\u0131n anti-kanser etkisi incelenmi\u015ftir.<\/p>\n

Kanser, insan ya\u015fam g\u00fcvenli\u011fini tehdit eden k\u00f6t\u00fc huylu hastal\u0131klardan biridir. \u00c7ok say\u0131da epidemiyolojik \u00e7al\u0131\u015fma, daha fazla turpgil sebze t\u00fcketmenin kanser riskini etkili bir \u015fekilde azaltabilece\u011fini ortaya koymu\u015ftur. \u0130zotiyosiyanat, turpgil sebzelerdeki kendi kendine enzimatik hidroliz \u00fcr\u00fcnlerinden biridir. Ara\u015ft\u0131rmalar, izotiyosiyanatlar\u0131n insan v\u00fccudundaki faz II enzimlerini aktive ederek faz I reaksiyonlar\u0131n\u0131n \u00fcr\u00fcnlerinin glukuronik asit ve glutatyon ile ba\u011flanmas\u0131na izin verdi\u011fini ve b\u00f6ylece kanserojenlerin v\u00fccuttan h\u0131zla at\u0131lmas\u0131n\u0131 sa\u011flad\u0131\u011f\u0131n\u0131 g\u00f6stermi\u015ftir. Bu nedenle, izotiyosiyanatlar t\u0131pta bir kanser inhibit\u00f6r\u00fc olarak kullan\u0131labilir. Qiamagu, Sincan'da glukozinolatlar, flavonoidler, izotiyosiyanatlar ve polisakkaritler gibi \u00e7e\u015fitli aktif bile\u015fenler i\u00e7eren t\u0131bbi ve yenilebilir bir bitkidir. Ancak, Qiamagu izotiyosiyanatlar\u0131n\u0131n geli\u015ftirilmesi ve kullan\u0131m\u0131 \u015fu anda yetersizdir. Enzimatik hidroliz \u015fu anda izotiyosiyanatlar\u0131n \u00e7\u0131kar\u0131lmas\u0131 i\u00e7in \u00e7evre dostu bir y\u00f6ntemdir. Enzimatik hidroliz ko\u015fullar\u0131n\u0131n optimize edilmesiyle, yaln\u0131zca di\u011fer ikincil metabolitlerin \u00fcretimi azalt\u0131lmakla kalmaz, ayn\u0131 zamanda hedef \u00fcr\u00fcn\u00fcn \u00fcretim h\u0131z\u0131 da h\u0131zland\u0131r\u0131labilir ve b\u00f6ylece verim art\u0131r\u0131labilir. Jang, Brassicaceae familyas\u0131nda endojen mirosinaz kullanarak izotiyosiyanatlar haz\u0131rlam\u0131\u015ft\u0131r. Endojen enzim y\u00f6ntemi basit bir s\u00fcrece sahip olsa da, bitkilerdeki mirosinaz tamamen serbest b\u0131rak\u0131lamaz, bu da uzun enzimatik hidroliz s\u00fcresine ve d\u00fc\u015f\u00fck verime neden olur. Endojen enzimlerle kar\u015f\u0131la\u015ft\u0131r\u0131ld\u0131\u011f\u0131nda, izotiyosiyanatlar\u0131 haz\u0131rlamak i\u00e7in eksojen enzimlerin kullan\u0131lmas\u0131, eksojen enzimlerin enzimatik hidroliz ko\u015fullar\u0131n\u0131 kontrol etmek daha kolayd\u0131r, bu da enzimatik hidroliz \u00fcr\u00fcnlerinin t\u00fcrlerindeki de\u011fi\u015fiklikleri y\u00f6nlendirmek ve \u00fcr\u00fcn miktar\u0131n\u0131 art\u0131rmak i\u00e7in faydal\u0131d\u0131r. Bu nedenle, harici enzimatik hidroliz ko\u015fullar\u0131n\u0131n optimize edilmesi, izotiyosiyanatlar\u0131n haz\u0131rlanmas\u0131n\u0131n anahtar\u0131d\u0131r.<\/p>\n

Enzimatik hidroliz \u00fcr\u00fcnlerinin olu\u015fumu sadece glukozinolat t\u00fcr\u00fcyle ilgili de\u011fildir, ayn\u0131 zamanda hidroliz s\u00fcresi, hidroliz s\u0131cakl\u0131\u011f\u0131, pH, bas\u0131n\u00e7 ve Fe2+ varl\u0131\u011f\u0131n\u0131 da i\u00e7erir. Latt \u00e9, \u00e7\u00f6zeltinin pH'\u0131 n\u00f6tr oldu\u011funda, glukozinolatlar\u0131n izotiyosiyanat olu\u015fturmas\u0131n\u0131n uygun oldu\u011funu bulmu\u015ftur. Bununla birlikte, pH asidik\/alkali oldu\u011funda veya siklik s\u00fclf\u00fcr karakteristik proteinler veya Fe2+ mevcut oldu\u011funda, enzimatik hidroliz \u00fcr\u00fcnleri b\u00fcy\u00fck miktarda nitril olu\u015fturacakt\u0131r. Yang, pH, EDTA ve askorbik asit fakt\u00f6rlerini analiz etmek i\u00e7in yan\u0131t y\u00fczeyi metodolojisini kullanm\u0131\u015f ve bu \u00fc\u00e7 fakt\u00f6r\u00fcn izotiyosiyanat\u0131n ekstraksiyon miktar\u0131 \u00fczerinde \u00f6nemli bir etkisi oldu\u011funu bulmu\u015ftur. Burmeister, askorbik asidin mirosinaz\u0131n aktif b\u00f6lgesindeki katalitik baz fonksiyonunun yerini alarak mirosinaz\u0131n katalitik \u00f6zelliklerini sergiledi\u011fini bulmu\u015ftur. Taze brokoliye uygun miktarda askorbik asit eklenmesi, mirosinaz aktivitesini \u00f6nemli \u00f6l\u00e7\u00fcde art\u0131rabilir ve b\u00f6ylece izotiyosiyanat \u00fcretimini art\u0131rabilir. Bu \u00e7al\u0131\u015fmada BRIe haz\u0131rlamak i\u00e7in eksojen mirosinaz (beyaz hardal tohumlar\u0131ndan elde edilen) par\u00e7alama y\u00f6ntemi kullan\u0131lm\u0131\u015ft\u0131r. Enzimatik hidroliz ko\u015fullar\u0131 tek fakt\u00f6rl\u00fc ve ortogonal deneylerle optimize edilmi\u015f ve BRIe'nin ekstraksiyonu \u00fczerinde \u00f6nemli etkisi olan d\u00f6rt fakt\u00f6r taranm\u0131\u015ft\u0131r: enzimatik hidroliz s\u00fcresi, enzimatik hidroliz s\u0131cakl\u0131\u011f\u0131, askorbik asit konsantrasyonu ve pH. BRIe i\u00e7in en uygun enzimatik hidroliz ko\u015fullar\u0131 \u015fu \u015fekilde belirlenmi\u015ftir: 20 \u03bc L mirosinaz ilavesi, 70 \u2103 reaksiyon s\u0131cakl\u0131\u011f\u0131, 3 saat reaksiyon s\u00fcresi, 6,5 pH, 0,1 mg\/g askorbik asit konsantrasyonu ve 2 g GLs dondurularak kurutulmu\u015f toz. Bu \u00e7al\u0131\u015fma i\u00e7in optimum enzimatik hidroliz s\u0131cakl\u0131\u011f\u0131n\u0131n 70 \u2103 olmas\u0131 ilgin\u00e7tir. Brokoliden bitki izotiyosiyanatlar\u0131n\u0131n enzimatik hidrolizi i\u00e7in s\u0131cakl\u0131k aral\u0131\u011f\u0131n\u0131n 20-60 \u2103 oldu\u011fu ve optimum enzimatik hidroliz s\u0131cakl\u0131\u011f\u0131n\u0131n 40 \u2103 oldu\u011fu; Wasabi k\u00f6klerinden izotiyosiyanatlar\u0131n \u00e7\u0131kar\u0131lmas\u0131 i\u00e7in s\u0131cakl\u0131k aral\u0131\u011f\u0131n\u0131n 30-90 \u2103 oldu\u011fu ve optimum enzimatik hidroliz s\u0131cakl\u0131\u011f\u0131n\u0131n 70 \u2103 oldu\u011fu ara\u015ft\u0131rma raporlar\u0131 vard\u0131r. Bu nedenle, mirosinaz i\u00e7in optimum s\u0131cakl\u0131k, men\u015fe t\u00fcr\u00fcne ba\u011fl\u0131 olarak de\u011fi\u015fir.<\/p>\n

Genellikle, olu\u015fan izotiyosiyanat t\u00fcrleri enzimatik hidroliz ko\u015fullar\u0131na ba\u011fl\u0131 olarak de\u011fi\u015fir. Chen ve ark. brokolideki enzimatik hidroliz \u00fcr\u00fcnlerini allil izotiyosiyanat, izob\u00fctil izotiyosiyanat, 1-butenil izotiyosiyanat, 4-metiltiyonitril olarak tan\u0131mlamak i\u00e7in endojen enzimatik hidrolizi kullanm\u0131\u015ft\u0131r, 5-metiltiyopentonitril, 3-(metiltiyo) propil izotiyosiyanat, b\u00fctil izotiyosiyanat, turp tiyosiyanat ve turp s\u00fclforafan\u0131 GC-MS kullanarak, en y\u00fcksek i\u00e7eri\u011fe sahip olan turp s\u00fclforafan\u0131. Yang, brokolideki enzimatik hidroliz \u00fcr\u00fcnlerini alil izotiyosiyanat, fenetil izotiyosiyanat, siklopentil izotiyosiyanat, fenilpropanonitril, 2,1-feniloksazolidinon ve tiroid uyar\u0131c\u0131 hormon olarak tan\u0131mlamak i\u00e7in eksojen enzimatik hidroliz kullanm\u0131\u015ft\u0131r. Bu \u00e7al\u0131\u015fma, GC-MS kullanarak BRIe i\u00e7in optimal enzimatik hidroliz ko\u015fullar\u0131n\u0131n bile\u015fimini analiz etmi\u015f ve 3-amino-2-oksazolidinon, 2-oksazolidinetiyon olmak \u00fczere enzimatik hidrolizle ilgili yedi \u00fcr\u00fcn tan\u0131mlam\u0131\u015ft\u0131r, 3-buten-1-ilizotiyosiyanat (BITC), fenil izotiyosiyanat (PEITC), 1-izotiyosiyanat propil ester, izotiyosiyanat izopropil ester ve 2-fenilizotiyosiyanat (2-PITC), bunlardan 4'\u00fc izotiyosiyanat t\u00fcrleriydi.<\/p>\n

Bir\u00e7ok \u00e7al\u0131\u015fma izotiyosiyanatlar\u0131n \u00e7e\u015fitli kanserlerin olu\u015fumunu \u00f6nleyebilece\u011fini g\u00f6stermi\u015ftir. Ara\u015ft\u0131rmalar PEITC'nin fare osteosarkom h\u00fccre hatt\u0131 K7M2'nin proliferasyonunu doza ba\u011fl\u0131 olarak inhibe etti\u011fini ve G2\/M h\u00fccre d\u00f6ng\u00fcs\u00fcn\u00fcn durmas\u0131na ve apoptoza neden olabilece\u011fini; BITC'nin gastrik adenokarsinom h\u00fccresi AGS'nin h\u00fccre canl\u0131l\u0131\u011f\u0131n\u0131 \u00f6nemli \u00f6l\u00e7\u00fcde azaltabilece\u011fini ve apoptoz ve otofajiyi ind\u00fckleyebilece\u011fini bulmu\u015ftur. Bununla birlikte, izotiyosiyanatlar\u0131n serviks kanseri \u00fczerindeki etkileri ve mekanizmalar\u0131 hakk\u0131nda \u00e7ok az rapor bulunmaktad\u0131r. Bu \u00e7al\u0131\u015fma, in vitro h\u00fccre modeli arac\u0131l\u0131\u011f\u0131yla BRIe'nin servikal kanser h\u00fccreleri Hela, Siha ve U14'\u00fcn proliferasyonunu zamana ve doza ba\u011fl\u0131 bir \u015fekilde \u00f6nemli \u00f6l\u00e7\u00fcde inhibe etti\u011fini bulmu\u015ftur. Bu \u00fc\u00e7 h\u00fccre tipinin 24 saatlik IC50 de\u011ferleri kar\u015f\u0131la\u015ft\u0131r\u0131ld\u0131\u011f\u0131nda, Hela h\u00fccrelerinin BRIe'ye en duyarl\u0131 h\u00fccreler oldu\u011fu sonucuna var\u0131lm\u0131\u015ft\u0131r. Kontrols\u00fcz h\u00fccre \u00e7o\u011falmas\u0131, h\u00fccre d\u00f6ng\u00fcs\u00fcn\u00fcn bozulmas\u0131na ba\u011fl\u0131 olan kanserin temel nedenidir. Ak\u0131\u015f sitometrisi analizi BRIe'nin Hela h\u00fccre d\u00f6ng\u00fcs\u00fcn\u00fc S ve G2\/M a\u015famalar\u0131nda durdurabildi\u011fini ortaya koymu\u015ftur. Kanser h\u00fccrelerinin metastaz\u0131 her zaman bir ara\u015ft\u0131rma noktas\u0131 ve klinik tedavi zorlu\u011fu olmu\u015ftur. \u00c7izme deneyleri sayesinde, BRIe'nin Hela h\u00fccrelerinin g\u00f6\u00e7\u00fcn\u00fc etkili bir \u015fekilde engelleyebildi\u011fi bulunmu\u015ftur. Hochest 33258 boyamas\u0131, BRIe'nin Hela h\u00fccre \u00e7ekirdeklerinde DNA yo\u011funla\u015fmas\u0131n\u0131 ind\u00fckleyebilece\u011fini ve \u00e7ekirdeklerde g\u00f6r\u00fcn\u00fcr gran\u00fcler floresan\u0131n g\u00f6zlenebilece\u011fini ve bunun apoptotik bir cisim oldu\u011funu d\u00fc\u015f\u00fcnd\u00fcrd\u00fc\u011f\u00fcn\u00fc ortaya koydu. Annexin V-FITC \u00e7ift boyama kullan\u0131larak yap\u0131lan ileri analiz, BRIe ile muamele edilen Hela h\u00fccrelerinin apoptoz oran\u0131n\u0131n 24 saat sonra artan konsantrasyonla artt\u0131\u011f\u0131n\u0131 ortaya koymu\u015ftur, bu da BRIe'nin Hela h\u00fccrelerinde apoptozu ind\u00fckledi\u011fini g\u00f6stermektedir. \u00d6zetle, BRIe \u00f6nemli bir anti servikal kanser aktivitesi sergilemektedir. GC-MS verilerine dayanarak, BITC, PEITC, izopropil izotiyosiyanat ve 2-PITC'nin, in vitro ve in vivo deneylerle daha fazla do\u011frulanmas\u0131 gereken ana aktif bile\u015fenleri olabilece\u011fi tahmin edilmektedir.<\/p>\n

\u00d6zetle, bu \u00e7al\u0131\u015fma ilk kez Chamagu'da izotiyosiyanatlar\u0131n eksojen enzimatik hidrolizi i\u00e7in en uygun haz\u0131rlama s\u00fcrecini belirlemi\u015ftir. GC-MS analizi yoluyla enzimatik hidrolizle ilgili yedi \u00fcr\u00fcn tan\u0131mlanm\u0131\u015ft\u0131r ve elde edilen enzimatik hidroliz \u00fcr\u00fcn\u00fc BRIe, servikal kanser h\u00fccrelerinin \u00e7o\u011falmas\u0131n\u0131 inhibe etme, h\u00fccre g\u00f6\u00e7\u00fcn\u00fc bask\u0131lama, h\u00fccre d\u00f6ng\u00fcs\u00fcn\u00fc durdurma ve apoptozu ind\u00fckleme yetene\u011fine sahiptir. Ara\u015ft\u0131rma, Chamagu bitki kaynaklar\u0131n\u0131n daha da geli\u015ftirilmesi ve kullan\u0131lmas\u0131 i\u00e7in bir referans temeli sa\u011flamaktad\u0131r. Ara\u015ft\u0131rma sonu\u00e7lar\u0131 2024 y\u0131l\u0131nda Natural Product Research and Development dergisinin 6. say\u0131s\u0131nda yay\u0131mlanm\u0131\u015ft\u0131r.<\/p>","protected":false},"excerpt":{"rendered":"

Enzymatic preparation and anti cervical cancer activity of Chamagu isothiocyanate In recent years, the incidence rate of cancer is increasing rapidly in China, and the prevention and treatment of cancer is urgent. Research shows that increasing the intake of cruciferous vegetables can effectively reduce the incidence rate of cancer. Brassicaceae vegetables contain abundant glucosinolates, also […]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"yoast_head":"\nEnzymatic preparation and anti cervical cancer activity of Chamagu isothiocyanate - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/tr\/samagu-i\u0307zoti\u0307osi\u0307yanat\/\" \/>\n<meta property=\"og:locale\" content=\"tr_TR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Enzymatic preparation and anti cervical cancer activity of Chamagu isothiocyanate - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"Enzymatic preparation and anti cervical cancer activity of Chamagu isothiocyanate In recent years, the incidence rate of cancer is increasing rapidly in China, and the prevention and treatment of cancer is urgent. 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