Research on the Chemical Composition and Antitumor Activity of Qiannian Jian
Homalomena occulta (Lour.) Schott As a member of the Araceae family, the genus Millennium Health has over 140 species, mainly distributed in Asia and the Americas, with Yunnan, Hainan, Guangxi, Guangdong and other regions in China being the main distribution areas. Qiannian Jian was included in the Chinese Pharmacopoeia in 2000 and is mainly used for the treatment of diseases such as wind cold and rheumatism. The Compendium of Materia Medica records: “It is shaped like a vine, several feet long, and has an extremely fragrant and strong aura. Traditionally, Qiannian Jian mainly uses its dried roots and stems as medicine, and is also commonly used by ethnic minorities. It has good therapeutic effects on spasms, numbness, rheumatism, rheumatoid arthritis, lower back and leg pain, gastrointestinal inflammation, and fractures. There are research reports that this plant contains volatile oils, sesquiterpenes, alkaloids, phenolic acids, and volatile oils, which have strong antioxidant, nematode killing, cytotoxic, and antibacterial activities, as well as a certain degree of anti-inflammatory effect. There are also studies showing that the sesquiterpenes contained in Millennium Health can promote the proliferation and differentiation of bone cells, indirectly inhibit the release of inflammatory factors such as nitric oxide, prostaglandin E2, and cyclooxygenase-2, and have potential anti osteoporosis effects. On the basis of previous research, this article further analyzes the chemical composition of Millennium Health, aiming to discover chemical components with anti-tumor physiological activity and lay the foundation for improving the medicinal value of Araceae plants.

This article mainly studies the chemical composition and anti-tumor effect of Qiannian Jian. Using medium pressure preparation liquid phase and Sephadex LH-20 10/11 separation methods, 10 compounds were isolated from Qiannian Jian alcohol extract solution. Except for compounds 7 and 8, all the other compounds isolated in this experiment were isolated from millennium plants for the first time.
The study found that compounds 2, 4, and 9 have strong toxicity to 4T1, A549, Hela, and HepG2 tumor cells. In vivo experiments on 4T1 tumor bearing mice, compounds 2, 4, and 9 showed strong effects on small tumor growth indicators (tumor volume, weight, etc.) compared to methotrexate treatment.
On the basis of previous research, this article focuses on the chemical composition, in vivo and in vitro anti-tumor effects, and other aspects of Qiannian Jian, aiming to discover the chemical components with anti-tumor physiological activity in Qiannian Jian, and further improve the medicinal value of Araceae plants.