{"id":8989,"date":"2024-08-15T15:47:29","date_gmt":"2024-08-15T15:47:29","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=8989"},"modified":"2024-08-15T15:47:29","modified_gmt":"2024-08-15T15:47:29","slug":"bn7-in-boju","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/hu\/bn7-boju-ban\/","title":{"rendered":"A BN7 endofita bakt\u00e9rium azonos\u00edt\u00e1sa Boju-ban, valamint biokontroll \u00e9s n\u00f6veked\u00e9sserkent\u0151 funkci\u00f3inak vizsg\u00e1lata"},"content":{"rendered":"<p>A BN7 endofita bakt\u00e9rium azonos\u00edt\u00e1sa Boju-ban, valamint biokontroll \u00e9s n\u00f6veked\u00e9sserkent\u0151 funkci\u00f3inak vizsg\u00e1lata<br \/>\nA n\u00f6v\u00e9nyi endofita bakt\u00e9riumok olyan mikroorganizmusok csoportj\u00e1t jelentik, amelyek eg\u00e9szs\u00e9ges n\u00f6v\u00e9nyi sz\u00f6vetekben vagy sejtekben \u00e9lnek \u00e9let\u00fck egy bizonyos szakasz\u00e1ban vagy teljes \u00e9letszakasz\u00e1ban, \u00e9s nem okoznak nyilv\u00e1nval\u00f3 betegs\u00e9gt\u00fcneteket a gazdaszervezetben. A n\u00f6v\u00e9nyek teljes n\u00f6veked\u00e9si folyamata a gazdan\u00f6v\u00e9ny \u00e9s az endofita k\u00f6z\u00f6ss\u00e9g \u00e9letfolyamata. Az endofit\u00e1k komplex \u00f6kol\u00f3giai kapcsolatokat alak\u00edtanak ki a gazdan\u00f6v\u00e9nyekkel \u00e9s az \u0151ket k\u00f6r\u00fclvev\u0151 \u00e9l\u0151 k\u00f6rnyezet\u00fckkel. Az endofita bakt\u00e9riumok k\u00e9pesek a gazdaszervezet\u00e9hez hasonl\u00f3 metabolitokat termelni, \u00e9s a n\u00f6v\u00e9nyeket felruh\u00e1zz\u00e1k a biotikus \u00e9s abiotikus stresszhat\u00e1soknak val\u00f3 ellen\u00e1ll\u00e1s k\u00e9pess\u00e9g\u00e9vel. Az endofita bakt\u00e9riumok \u00e9s a n\u00f6v\u00e9nyek k\u00f6z\u00f6tti hossz\u00fa t\u00e1v\u00fa k\u00f6lcs\u00f6nhat\u00e1s sor\u00e1n az endofita bakt\u00e9riumokra jellemz\u0151, hogy fokozz\u00e1k a gazdan\u00f6v\u00e9ny boml\u00e1s\u00e1t \u00e9s az \u00e1sv\u00e1nyi elemek, p\u00e9ld\u00e1ul a foszfor \u00e9s a k\u00e1lium felsz\u00edv\u00f3d\u00e1s\u00e1t. Az olyan multifunkcion\u00e1lis bakt\u00e9riumt\u00f6rzsek sz\u0171r\u00e9se, amelyek antagonista hat\u00e1ssal rendelkeznek a n\u00f6v\u00e9nyi k\u00f3rokoz\u00f3kra, valamint k\u00e9pesek a foszfor \u00e9s a k\u00e1lium hidroliz\u00e1l\u00e1s\u00e1ra \u00e9s a ny\u00falv\u00e1nytermel\u00e9sre, nagy lehet\u0151s\u00e9geket rejt mag\u00e1ban a mez\u0151gazdas\u00e1gban val\u00f3 alkalmaz\u00e1s szempontj\u00e1b\u00f3l.<\/p>\n<p>A krizant\u00e9mok h\u0151tiszt\u00edt\u00f3, h\u0151talan\u00edt\u00f3 \u00e9s antioxid\u00e1ci\u00f3s, valamint antibakteri\u00e1lis, gyullad\u00e1scs\u00f6kkent\u0151, fogy\u00f3k\u00far\u00e1s \u00e9s a magas h\u00fagysavtartalm\u00fa v\u00e9rrel kapcsolatos betegs\u00e9gek enyh\u00edt\u00e9s\u00e9re alkalmas hat\u00e1s\u00faak. Mint a n\u00e9gy f\u0151 gy\u00f3gy\u00e1szati krizant\u00e9m egyike, kutat\u00f3csoportunk el\u0151zetes vizsg\u00e1latokat v\u00e9gzett endofita gomb\u00e1ival kapcsolatban. Ebben a k\u00eds\u00e9rletben a Chrysanthemum morifoliumban l\u00e9v\u0151 BN7 endofita bakt\u00e9rium antibakteri\u00e1lis, foszf\u00e1told\u00f3, k\u00e1liumold\u00f3, n\u00f6veked\u00e9st el\u0151seg\u00edt\u0151, cellul\u00f3zbont\u00f3 \u00e9s antioxid\u00e1ns aktivit\u00e1s\u00e1t vizsg\u00e1ltuk, \u00e9s el\u0151zetesen azonos\u00edtottuk, megalapozva ezzel a biol\u00f3giai kontrollforr\u00e1sok gazdag\u00edt\u00e1s\u00e1t \u00e9s a biotr\u00e1gy\u00e1k fejleszt\u00e9s\u00e9t.<\/p>\n<p>Az 1970-es \u00e9vek v\u00e9g\u00e9n Dobereiner \u00e9s munkat\u00e1rsai felfedezt\u00e9k, hogy a nitrog\u00e9nk\u00f6t\u0151 csig\u00e1k k\u00e9pesek el\u0151seg\u00edteni a nem h\u00fcvelyes n\u00f6v\u00e9nyek n\u00f6veked\u00e9s\u00e9t; az 1980-as \u00e9vek v\u00e9g\u00e9n a Philom Bios, egy kanadai v\u00e1llalat elkezdte gy\u00e1rtani \u00e9s \u00e9rt\u00e9kes\u00edteni a foszf\u00e1told\u00f3 bakt\u00e9riumokat, nitrog\u00e9nk\u00f6t\u0151 bakt\u00e9riumokat, valamint a foszf\u00e1t- \u00e9s nitrog\u00e9nk\u00f6t\u0151 bakt\u00e9riumok kever\u00e9k\u00e9t, amelyek 10% \u00e9s 15% k\u00f6z\u00f6tti term\u00e9shozamot tudtak n\u00f6velni olyan n\u00f6v\u00e9nyek eset\u00e9ben, mint a b\u00faza, a repce \u00e9s a bors\u00f3. Ezt k\u00f6vet\u0151en a n\u00f6v\u00e9nyekben tal\u00e1lhat\u00f3 endofita bakt\u00e9riumok fokozatosan forr\u00f3 kutat\u00e1si t\u00e9m\u00e1v\u00e1 v\u00e1ltak mind hazai, mind nemzetk\u00f6zi szinten. 2009-ben Cheng \u00e9s munkat\u00e1rsai a Huazhong Buildingb\u0151l sz\u00e1rmaz\u00f3 PCE45 endofita bakt\u00e9riumot vizsg\u00e1lt\u00e1k, amely antibakteri\u00e1lis peptidet PCP-1-et termelt, amely g\u00e1tl\u00f3 hat\u00e1ssal volt k\u00fcl\u00f6nb\u00f6z\u0151 k\u00f3rokoz\u00f3kra, p\u00e9ld\u00e1ul a kukorica g\u00f6rb\u00fcleti boedre \u00e9s a rizs blast gomb\u00e1ra (Pyricularia oryzae) Cav. 2010-ben Bressan \u00e9s munkat\u00e1rsai hat endofita Bacillus-fajt izol\u00e1ltak, amelyek k\u00e9pesek kitin\u00e1zt termelni kukorica n\u00f6v\u00e9nyi sz\u00f6vetekb\u0151l, \u00e9s mindegyik\u00fck g\u00e1tl\u00f3 hat\u00e1st mutatott a Fusarium graminearumra. 2015-ben Zhao et al. 36 foszforszolubiliz\u00e1l\u00f3 endofita bakt\u00e9riumot sz\u0171rte sz\u00f3jacsom\u00f3kb\u00f3l, amelyek k\u00f6z\u00fcl a DD291 t\u00f6rzsnek ak\u00e1r 452 mg\/l oldhat\u00f3 foszfortartalma is volt etanolos oldatban, \u00e9s n\u00e9h\u00e1ny foszforszolubiliz\u00e1l\u00f3 t\u00f6rzsnek el\u0151seg\u00edt\u0151 hat\u00e1sa volt a sz\u00f3jabab n\u00f6veked\u00e9s\u00e9re. 2015-ben Kang \u00e9s munkat\u00e1rsai meg\u00e1llap\u00edtott\u00e1k, hogy a lucern\u00e1ban tal\u00e1lhat\u00f3 ASR16 \u00e9s ALR33 endofita bakt\u00e9riumok n\u00f6v\u00e9nyi n\u00f6veked\u00e9si hormont \u00e9s sziderofort termelnek, foszforszolubiliz\u00e1l\u00f3 aktivit\u00e1ssal rendelkeznek, \u00e9s jelent\u0151s el\u0151seg\u00edt\u0151 hat\u00e1ssal vannak a lucerna n\u00f6veked\u00e9s\u00e9re. 2018-ban Gao \u00e9s munkat\u00e1rsai 57 endofita bakt\u00e9riumot izol\u00e1ltak Niwala vadrizsb\u0151l, amelyek k\u00f6z\u00fcl 44 endofita nitrog\u00e9nk\u00f6t\u0151 bakt\u00e9rium k\u00e9pes volt lignin termel\u00e9s\u00e9re, 25 t\u00f6rzs pedig k\u00fcl\u00f6nb\u00f6z\u0151 m\u00e9rt\u00e9kben k\u00e1liumszolubiliz\u00e1l\u00f3 k\u00e9pess\u00e9ggel rendelkezett. 2018-ban Bai \u00e9s munkat\u00e1rsai vadon term\u0151 Polygonatum sibiricum gy\u00f6kerekb\u0151l izol\u00e1ltak egy endofita Bacillus HJ-1-et, amely jelent\u0151s antibakteri\u00e1lis aktivit\u00e1st mutatott Salmonella typhimurium, Pseudomonas aeruginosa \u00e9s Bacillus thuringiensis ellen.<\/p>\n<p>Jelenleg viszonylag kev\u00e9s kutat\u00e1s folyik a Chrysanthemum morifolium endofita bakt\u00e9riumair\u00f3l, \u00e9s kev\u00e9s jelent\u00e9s sz\u00f3l a Chrysanthemum morifolium endofita bakt\u00e9riumai \u00e1ltal t\u00f6rt\u00e9n\u0151 n\u00f6veked\u00e9s el\u0151seg\u00edt\u00e9s\u00e9r\u0151l. Ebben a vizsg\u00e1latban a Chrysanthemum morifolium BN7 endofitikus bakt\u00e9rium\u00e1nak antagonista hat\u00e1sa hat n\u00f6v\u00e9nyi k\u00f3rokoz\u00f3val szemben mutatkozott, k\u00f6zt\u00fck a kukorica g\u00f6rbe gomba CY1 ellen, a legnagyobb g\u00e1tl\u00e1si ar\u00e1nyt 68%-vel a b\u00faza Fusarium graminearum FM1 ellen mutatta. Ez a t\u00f6rzs bizonyos k\u00e9pess\u00e9gekkel rendelkezik a foszfor \u00e9s a k\u00e1lium hidrol\u00edzis\u00e9re, valamint az IAA termel\u00e9s\u00e9re is, 108,38, 32,03 \u00e9s 164,39 mg\/L koncentr\u00e1ci\u00f3val. A BN7 t\u00f6rzs k\u00e9pess\u00e9ge a szervetlen foszfor hidroliz\u00e1l\u00e1s\u00e1ra (68,71 mg\/l) k\u00f6r\u00fclbel\u00fcl h\u00e1romszorosa az endofita Bacillus subtilis ZS-3 (20,16 mg\/l) k\u00e1mforf\u00e1kban termelt endofita Bacillus subtilis ZS-3-nak, ami \u00f6sszef\u00fcgg\u00e9sben lehet az ut\u00f3bbi r\u00f6videbb teny\u00e9szt\u00e9si idej\u00e9vel (24 \u00f3ra); tov\u00e1bb\u00e1 a BN7 t\u00f6rzs hossz\u00fa hormonok termel\u00e9s\u00e9re val\u00f3 k\u00e9pess\u00e9ge n\u00e9gyszerese az N8 t\u00f6rzs\u00e9nek (41,66 mg\/l), amelyet Gao Chen \u00e9s t\u00e1rsai a leger\u0151sebb hossz\u00fa hormontermel\u0151 k\u00e9pess\u00e9ggel rendelkez\u0151 t\u00f6rzsnek v\u00e1lasztottak. A BN7 t\u00f6rzs a cellul\u00f3z lebont\u00e1s\u00e1ra is k\u00e9pes, a harmadik napon a CMCase \u00e9s FPase enzimaktivit\u00e1sa 168,78U\/ml, illetve 79,87U\/ml volt. Ezen k\u00edv\u00fcl ak\u00e1r 82,13% DPPH-szabadgy\u00f6kfog\u00f3 aktivit\u00e1ssal is rendelkezik, ami j\u00f3val magasabb, mint a kor\u00e1bban vizsg\u00e1lt BJF10 endofita gomba DPPH-gy\u00f6kfog\u00f3 aktivit\u00e1sa (67,7%). A morfol\u00f3giai jellemz\u0151k, fiziol\u00f3giai \u00e9s biok\u00e9miai k\u00eds\u00e9rletek, valamint a 16S rDNS-szekvencia elemz\u00e9se alapj\u00e1n a BN7 t\u00f6rzset Bacillus subtilis-k\u00e9nt azonos\u00edtottuk. A Bacillus subtilis BN7 teh\u00e1t egy endofita bakt\u00e9rium, amely k\u00e9pes a n\u00f6v\u00e9nyi k\u00f3rokoz\u00f3k antagoniz\u00e1l\u00e1s\u00e1ra, a foszfor \u00e9s a k\u00e1lium hidroliz\u00e1l\u00e1s\u00e1ra, valamint az IAA termel\u00e9s\u00e9re, \u00e9s potenci\u00e1lisan fejleszthet\u0151 biokontroll \u00e9s n\u00f6veked\u00e9sserkent\u0151 szerk\u00e9nt.<\/p>","protected":false},"excerpt":{"rendered":"<p>A BN7 endofita bakt\u00e9rium azonos\u00edt\u00e1sa Boju-ban, valamint biokontroll \u00e9s n\u00f6veked\u00e9sserkent\u0151 funkci\u00f3inak vizsg\u00e1lata A n\u00f6v\u00e9nyi endofita bakt\u00e9riumok olyan mikroorganizmusok csoportj\u00e1t jelentik, amelyek eg\u00e9szs\u00e9ges n\u00f6v\u00e9nyi sz\u00f6vetekben vagy sejtekben \u00e9lnek \u00e9let\u00fck egy bizonyos szakasz\u00e1ban vagy teljes \u00e9lettartamuk sor\u00e1n, \u00e9s nem okoznak nyilv\u00e1nval\u00f3 betegs\u00e9gt\u00fcneteket a gazdaszervezetben. 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