{"id":8490,"date":"2024-08-14T15:23:39","date_gmt":"2024-08-14T15:23:39","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=8490"},"modified":"2024-08-14T15:23:39","modified_gmt":"2024-08-14T15:23:39","slug":"green-mulberry","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/hu\/zold-eperfa\/","title":{"rendered":"Tanulm\u00e1ny a z\u00f6ld eperf\u00e1b\u00f3l sz\u00e1rmaz\u00f3 antoci\u00e1nok elv\u00e1laszt\u00e1s\u00e1r\u00f3l \u00e9s el\u0151\u00e1ll\u00edt\u00e1s\u00e1r\u00f3l nagy sebess\u00e9g\u0171 ellen\u00e1ram\u00fa kromatogr\u00e1fi\u00e1val, valamint antioxid\u00e1ns aktivit\u00e1sukr\u00f3l"},"content":{"rendered":"

Tanulm\u00e1ny a z\u00f6ld eperf\u00e1b\u00f3l sz\u00e1rmaz\u00f3 antoci\u00e1nok elv\u00e1laszt\u00e1s\u00e1r\u00f3l \u00e9s el\u0151\u00e1ll\u00edt\u00e1s\u00e1r\u00f3l nagy sebess\u00e9g\u0171 ellen\u00e1ram\u00fa kromatogr\u00e1fi\u00e1val, valamint antioxid\u00e1ns aktivit\u00e1sukr\u00f3l
\nAz eperfa, mint az eperfaf\u00e9l\u00e9k csal\u00e1dj\u00e1ba tartoz\u00f3 eperfa gy\u00fcm\u00f6lcse, t\u00e1panyagokban gazdag, \u00e9s k\u00fcl\u00f6nb\u00f6z\u0151 hat\u00e1sai vannak, p\u00e9ld\u00e1ul r\u00e1kellenes, antioxid\u00e1ns, f\u00e1jdalomcsillap\u00edt\u00f3 \u00e9s gyullad\u00e1scs\u00f6kkent\u0151, valamint a fekete haj sz\u00e9ps\u00e9ge. A z\u00f6ld eperfa, m\u00e1s n\u00e9ven s\u00e1rga eperfa, \u00e9retlen eperf\u00e1ra utal. A hagyom\u00e1nyos k\u00ednai gy\u00f3gy\u00e1szatban a z\u00f6ld eperf\u00e1t k\u00f6nnyebb szedni, sz\u00e1ll\u00edtani \u00e9s t\u00e1rolni, mint az \u00e9rett eperf\u00e1t, \u00e9s antoci\u00e1n-, katechin- \u00e9s epikatekin-tartalma sokkal magasabb, mint az \u00e9rett eperf\u00e1\u00e9. Hazai \u00e9s nemzetk\u00f6zi tanulm\u00e1nyok folynak az antoci\u00e1nok kivon\u00e1s\u00e1r\u00f3l \u00e9s izol\u00e1l\u00e1s\u00e1r\u00f3l k\u00fcl\u00f6nb\u00f6z\u0151 n\u00f6v\u00e9nyekb\u0151l, p\u00e9ld\u00e1ul sz\u0151l\u0151magb\u00f3l, r\u00f3zs\u00e1b\u00f3l, kukorica selyemb\u0151l, \u00e1fony\u00e1b\u00f3l, kaka\u00f3babb\u00f3l, stb. Liu \u00e9s munkat\u00e1rsai LSA-10 gyant\u00e1t haszn\u00e1ltak a galagony\u00e1b\u00f3l sz\u00e1rmaz\u00f3 antoci\u00e1nok d\u00fas\u00edt\u00e1s\u00e1ra \u00e9s tiszt\u00edt\u00e1s\u00e1ra; Chen Wenliang \u00e9s munkat\u00e1rsai membr\u00e1nszepar\u00e1ci\u00f3t \u00e9s sz\u0171r\u00e9st haszn\u00e1ltak az oligomer antoci\u00e1nok sz\u0151l\u0151magb\u00f3l t\u00f6rt\u00e9n\u0151 elv\u00e1laszt\u00e1s\u00e1ra, ami 65% oligomer antoci\u00e1n tartalm\u00fa antoci\u00e1nokat eredm\u00e9nyezett, de nem kaptak monomer elv\u00e1laszt\u00e1s\u00fa komponenseket; Glavnik \u00e9s munkat\u00e1rsai. nagy teljes\u00edtm\u00e9ny\u0171 v\u00e9konyr\u00e9teg-kromatogr\u00e1fi\u00e1val elemezte a jap\u00e1n tigriskan\u00e1l antoci\u00e1njait, \u00e9s a nyers kivonat\u00e1b\u00f3l monomereket, dekamereket \u00e9s B-t\u00edpus\u00fa antoci\u00e1nokat azonos\u00edtott. Ezt a m\u00f3dszert azonban neh\u00e9z elk\u00e9sz\u00edteni; Sun \u00e9s munkat\u00e1rsai f\u00e9lpreparat\u00edv nagy teljes\u00edtm\u00e9ny\u0171 folyad\u00e9kkromatogr\u00e1fi\u00e1t haszn\u00e1ltak az antoci\u00e1nok sz\u0151l\u0151magb\u00f3l t\u00f6rt\u00e9n\u0151 elv\u00e1laszt\u00e1s\u00e1ra, de az el\u0151kezel\u00e9s bonyolult volt, \u00e9s az elv\u00e1laszt\u00e1si hozam alacsony volt; Ez a cikk a nagy sebess\u00e9g\u0171 ellen\u00e1ram\u00fa kromatogr\u00e1fi\u00e1t (HSCCC) v\u00e1lasztja, amely nagy elv\u00e1laszt\u00e1si kapacit\u00e1ssal rendelkezik, t\u00e9teles el\u0151k\u00e9sz\u00edt\u00e9sre \u00e9s elv\u00e1laszt\u00e1sra haszn\u00e1lhat\u00f3, k\u00f6nnyen kezelhet\u0151 \u00e9s nagy elv\u00e1laszt\u00e1si hat\u00e9konys\u00e1ggal rendelkezik.
\nA procianidinek (PC), mint egyfajta polifenolos vegy\u00fclet, sz\u00e9les k\u00f6rben jelen vannak a n\u00f6v\u00e9nyekben. F\u0151leg katechinekb\u0151l, epikatekinb\u0151l (EC), epigallokatechinekb\u0151l (EGC) \u00e9s epikatekin-gall\u00e1tokb\u00f3l (ECG) \u00e1llnak, \u00e9s er\u0151s antioxid\u00e1ns hat\u00e1ssal rendelkeznek. E munka c\u00e9lja, hogy nagy sebess\u00e9g\u0171 ellen\u00e1ram\u00fa kromatogr\u00e1fi\u00e1t alkalmazzon a z\u00f6ld eperf\u00e1b\u00f3l sz\u00e1rmaz\u00f3 antoci\u00e1nok elk\u00fcl\u00f6n\u00edt\u00e9s\u00e9re \u00e9s tiszt\u00edt\u00e1s\u00e1ra. Nagy teljes\u00edtm\u00e9ny\u0171 folyad\u00e9kkromatogr\u00e1fi\u00e1t (HPLC) fogunk haszn\u00e1lni az egyes komponensek k\u00e9miai \u00f6sszetev\u0151inek azonos\u00edt\u00e1s\u00e1ra \u00e9s tisztas\u00e1guk elemz\u00e9s\u00e9re; a DPPH-gy\u00f6k\u00f6k, ABTS+gy\u00f6k\u00f6k \u00e9s hidroxilgy\u00f6k\u00f6k elnyel\u0151 k\u00e9pess\u00e9g\u00e9nek vizsg\u00e1lat\u00e1val az egyes komponensek antioxid\u00e1ns kapacit\u00e1s\u00e1nak er\u0151ss\u00e9g\u00e9t lehet t\u00fckr\u00f6zni. Ez a munka kiterjeszti az eperfa m\u00e9lyrehat\u00f3 feldolgoz\u00e1s\u00e1nak \u00e9s felhaszn\u00e1l\u00e1s\u00e1nak k\u00f6r\u00e9t, ir\u00e1nyt ad a z\u00f6ld eperfa antoci\u00e1njainak ipari el\u0151\u00e1ll\u00edt\u00e1s\u00e1hoz, \u00e9s bizonyos referencia\u00e9rt\u00e9kkel b\u00edr az eperfa mint gy\u00f3gy\u00e1szati er\u0151forr\u00e1s fejleszt\u00e9s\u00e9hez \u00e9s felhaszn\u00e1l\u00e1s\u00e1hoz.<\/p>\n

\u200d<\/p>\n

A z\u00f6ld eperf\u00e1t nyersanyagk\u00e9nt haszn\u00e1lva a nyers kivonatot extrakci\u00f3val, zs\u00edrtalan\u00edt\u00e1ssal, szennyez\u0151d\u00e9sek elt\u00e1vol\u00edt\u00e1s\u00e1val \u00e9s extrakci\u00f3s d\u00fas\u00edt\u00e1ssal nyert\u00e9k a k\u00eds\u00e9rleti k\u00f6r\u00fclm\u00e9nyek k\u00f6z\u00f6tt. A HSCCC elv\u00e1laszt\u00e1s\u00e1hoz \u00e9s tiszt\u00edt\u00e1s\u00e1hoz az optim\u00e1lis old\u00f3szerrendszer n-hex\u00e1n-etil-acet\u00e1t-v\u00edz (1:20:20, V\/V\/V) volt. 200 mg z\u00f6ld eperfa antoci\u00e1n nyers kivonatot HSCCC seg\u00edts\u00e9g\u00e9vel \u00f6t komponensre v\u00e1lasztottunk sz\u00e9t, nevezetesen F1 (28,5 mg), F2 (10,43 mg), F3 (6,85 mg), F4 (10,65 mg) \u00e9s F5 (13,65 mg). Minden komponens tartalmazott katekineket, antoci\u00e1n B2-t, antoci\u00e1n A1-et stb. K\u00f6z\u00fcl\u00fck az F4 epigallokatechin-gall\u00e1tot tartalmazott, 95,05% tisztas\u00e1ggal; az F5 katechint tartalmazott 73,75% tisztas\u00e1ggal. Az elk\u00fcl\u00f6n\u00edtett \u00f6sszetev\u0151k mindegyike rendelkezik bizonyos antioxid\u00e1ns aktivit\u00e1ssal. A Vc-t kontrollk\u00e9nt haszn\u00e1lva a DPPH szabad gy\u00f6kfog\u00f3 k\u00e9pess\u00e9g a k\u00f6vetkez\u0151: Vc>F5>F4>F2>F3>F1>z\u00f6ld eperfa nyers kivonata; ABTS+szabadgy\u00f6kfog\u00f3 k\u00e9pess\u00e9g: Vc>F5>F4>F2>F3>F1> z\u00f6ld eperfa nyers kivonata; hidroxil gy\u00f6kfog\u00f3 k\u00e9pess\u00e9g: F1>F5>Vc>F4>F2>z\u00f6ld eperfa nyers kivonata>F3. A HSCCC nagy el\u0151k\u00e9sz\u00edt\u00e9si kapacit\u00e1ssal rendelkezik, \u00e9s nincs csomagol\u00f3anyag a kromatogr\u00e1fi\u00e1s oszlopban. Ebben a k\u00eds\u00e9rletben a nyers kivonat egyszer elv\u00e1laszthat\u00f3, hogy \u00f6t komponenst kapjunk, \u00e9s egyes komponensek nagy tisztas\u00e1g\u00fa monomer anyagokkal \u00e9s er\u0151s antioxid\u00e1ns aktivit\u00e1ssal rendelkeznek. A cikk kutat\u00e1si tartalma \u00faj \u00f6tleteket nyitott az antoci\u00e1nok el\u0151\u00e1ll\u00edt\u00e1s\u00e1ra, \u00e9s elm\u00e9leti t\u00e1mogat\u00e1st ny\u00fajtott a z\u00f6ld eperf\u00e1ban l\u00e9v\u0151 antoci\u00e1nok gy\u00f3gyhat\u00e1s\u00e1nak tanulm\u00e1nyoz\u00e1s\u00e1hoz.<\/p>","protected":false},"excerpt":{"rendered":"

Study on the Separation and Preparation of Anthocyanins from Green Mulberry by High Speed Counter Current Chromatography and Their Antioxidant Activity Mulberry, as the fruit of mulberry trees in the mulberry family, is rich in nutrients and has various effects such as anti-cancer, antioxidant, analgesic and anti-inflammatory, and beauty of black hair. Green mulberries, also […]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"\nStudy on the Separation and Preparation of Anthocyanins from Green Mulberry by High Speed Counter Current Chromatography and Their Antioxidant Activity - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/hu\/zold-eperfa\/\" \/>\n<meta property=\"og:locale\" content=\"hu_HU\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Study on the Separation and Preparation of Anthocyanins from Green Mulberry by High Speed Counter Current Chromatography and Their Antioxidant Activity - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"Study on the Separation and Preparation of Anthocyanins from Green Mulberry by High Speed Counter Current Chromatography and Their Antioxidant Activity Mulberry, as the fruit of mulberry trees in the mulberry family, is rich in nutrients and has various effects such as anti-cancer, antioxidant, analgesic and anti-inflammatory, and beauty of black hair. 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Green mulberries, also […]","og_url":"https:\/\/longchangextracts.com\/hu\/zold-eperfa\/","og_site_name":"China Chemical Manufacturer","article_published_time":"2024-08-14T15:23:39+00:00","author":"longcha9","twitter_card":"summary_large_image","twitter_misc":{"Szerz\u0151:":"longcha9","Becs\u00fclt olvas\u00e1si id\u0151":"3 perc"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/longchangextracts.com\/green-mulberry\/","url":"https:\/\/longchangextracts.com\/green-mulberry\/","name":"Tanulm\u00e1ny az antoci\u00e1nok elv\u00e1laszt\u00e1sa \u00e9s el\u0151\u00e1ll\u00edt\u00e1sa a z\u00f6ld eperf\u00e1b\u00f3l nagy sebess\u00e9g\u0171 ellen\u00e1ram\u00fa kromatogr\u00e1fi\u00e1val \u00e9s antioxid\u00e1ns aktivit\u00e1suk - K\u00edna K\u00e9miai Gy\u00e1rt\u00f3","isPartOf":{"@id":"https:\/\/longchangextracts.com\/#website"},"datePublished":"2024-08-14T15:23:39+00:00","dateModified":"2024-08-14T15:23:39+00:00","author":{"@id":"https:\/\/longchangextracts.com\/#\/schema\/person\/80933ac6926ad63fea74ec33f8d04869"},"breadcrumb":{"@id":"https:\/\/longchangextracts.com\/green-mulberry\/#breadcrumb"},"inLanguage":"hu","potentialAction":[{"@type":"ReadAction","target":["https:\/\/longchangextracts.com\/green-mulberry\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/longchangextracts.com\/green-mulberry\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/longchangextracts.com\/"},{"@type":"ListItem","position":2,"name":"Study on the Separation and Preparation of Anthocyanins from Green Mulberry by High Speed Counter Current Chromatography and Their Antioxidant Activity"}]},{"@type":"WebSite","@id":"https:\/\/longchangextracts.com\/#website","url":"https:\/\/longchangextracts.com\/","name":"K\u00edna Vegyi Gy\u00e1rt\u00f3","description":"","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/longchangextracts.com\/?s={search_term_string}"},"query-input":"required name=search_term_string"}],"inLanguage":"hu"},{"@type":"Person","@id":"https:\/\/longchangextracts.com\/#\/schema\/person\/80933ac6926ad63fea74ec33f8d04869","name":"longcha9","image":{"@type":"ImageObject","inLanguage":"hu","@id":"https:\/\/longchangextracts.com\/#\/schema\/person\/image\/","url":"https:\/\/secure.gravatar.com\/avatar\/c1e84ee0759373263198c58e8f338f80?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/c1e84ee0759373263198c58e8f338f80?s=96&d=mm&r=g","caption":"longcha9"}}]}},"_links":{"self":[{"href":"https:\/\/longchangextracts.com\/hu\/wp-json\/wp\/v2\/posts\/8490"}],"collection":[{"href":"https:\/\/longchangextracts.com\/hu\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/longchangextracts.com\/hu\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/longchangextracts.com\/hu\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/longchangextracts.com\/hu\/wp-json\/wp\/v2\/comments?post=8490"}],"version-history":[{"count":1,"href":"https:\/\/longchangextracts.com\/hu\/wp-json\/wp\/v2\/posts\/8490\/revisions"}],"predecessor-version":[{"id":8491,"href":"https:\/\/longchangextracts.com\/hu\/wp-json\/wp\/v2\/posts\/8490\/revisions\/8491"}],"wp:attachment":[{"href":"https:\/\/longchangextracts.com\/hu\/wp-json\/wp\/v2\/media?parent=8490"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/longchangextracts.com\/hu\/wp-json\/wp\/v2\/categories?post=8490"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/longchangextracts.com\/hu\/wp-json\/wp\/v2\/tags?post=8490"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}