{"id":7966,"date":"2024-08-11T09:57:23","date_gmt":"2024-08-11T09:57:23","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=7966"},"modified":"2024-08-11T09:57:23","modified_gmt":"2024-08-11T09:57:23","slug":"gougu-leaves","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/hu\/gougu-levelek\/","title":{"rendered":"A lip\u00e1z g\u00e1tl\u00f3 akt\u00edv komponensek jellemz\u00e9se a Gougu levelekben v\u00e9konyr\u00e9teg-kromatogr\u00e1fi\u00e1s bioautogr\u00e1fi\u00e1s MSn m\u00f3dszerrel"},"content":{"rendered":"

A lip\u00e1z g\u00e1tl\u00f3 akt\u00edv komponensek jellemz\u00e9se a Gougu levelekben v\u00e9konyr\u00e9teg-kromatogr\u00e1fi\u00e1s bioautogr\u00e1fi\u00e1s MSn m\u00f3dszerrel
\nAz Ilex cornuta Lindl Ex Paxt az Ilex csal\u00e1dba tartoz\u00f3 Ilex nemzets\u00e9gbe tartoz\u00f3, K\u00edn\u00e1ban sz\u00e9les k\u00f6rben elterjedt n\u00f6v\u00e9ny. A Gougu sz\u00e1r\u00edtott levelei a Tang-dinasztia \u00f3ta sz\u00e1mos gy\u00f3gyn\u00f6v\u00e9nymonogr\u00e1fi\u00e1ban szerepelnek, \u00e9s t\u00f6bb mint ezer\u00e9ves gy\u00f3gy\u00e1szati m\u00faltra tekint vissza. A K\u00ednai N\u00e9pk\u00f6zt\u00e1rsas\u00e1g gy\u00f3gyszerk\u00f6nyve (2020-as kiad\u00e1s, 1. k\u00f6tet) szerint a Ligusticum barbarum leveleinek \u00edze keser\u0171, h\u0171v\u00f6s jelleg\u0171. A m\u00e1j- \u00e9s a vese meridi\u00e1nokhoz tartozik, \u00e9s funkci\u00f3i a h\u0151tiszt\u00edt\u00e1s \u00e9s a jin t\u00e1pl\u00e1l\u00e1sa, a ves\u00e9k jav\u00e1ra \u00e9s a m\u00e1j megnyugtat\u00e1sa. Haszn\u00e1lj\u00e1k tuberkul\u00f3zisos v\u00e9rz\u00e9scsillap\u00edt\u00e1s, csontg\u0151z\u00f6lg\u00e9s \u00e9s h\u0151hull\u00e1mok, sz\u00e9d\u00fcl\u00e9s \u00e9s sz\u00e9d\u00fcl\u00e9s stb. eset\u00e9n. A n\u00e9pi kult\u00far\u00e1ban a Gougu levelek h\u0151tiszt\u00edt\u00f3 hat\u00e1sa miatt zsenge leveleiket Kuding teak\u00e9nt f\u0151zik \u00e9s fogyasztj\u00e1k. A modern kutat\u00e1sok kimutatt\u00e1k, hogy a Cornus officinalis levelei gazdagok triterpenoidokban \u00e9s szaponinjaikban, amelyek a f\u0151 hat\u00f3anyagai. Ezen k\u00edv\u00fcl vannak k\u00fcl\u00f6nb\u00f6z\u0151 \u00f6sszetev\u0151i, mint p\u00e9ld\u00e1ul flavonoidok, polifenolok, szterolok stb., amelyeknek k\u00fcl\u00f6nb\u00f6z\u0151 hat\u00e1sai vannak, mint p\u00e9ld\u00e1ul a v\u00e9rzs\u00edrok cs\u00f6kkent\u00e9se, a sz\u00edvizom iszk\u00e9mi\u00e1ja elleni v\u00e9delem, antioxid\u00e1ci\u00f3, immunszuppresszi\u00f3 \u00e9s term\u00e9kenys\u00e9g elleni v\u00e9delem. A legt\u00f6bb fogy\u00f3k\u00far\u00e1s gy\u00f3gyszer jelent\u00e9ktelen hat\u00e9konys\u00e1ga \u00e9s jelent\u0151s mell\u00e9khat\u00e1sai miatt a Ligusticum barbarum levelek lipidcs\u00f6kkent\u0151 hat\u00e1sa fokozatosan felkeltette az emberek figyelm\u00e9t. A tud\u00f3sok itthon \u00e9s k\u00fclf\u00f6ld\u00f6n kiterjedt kutat\u00e1sokat v\u00e9geztek a Ligusticum barbarum levelek k\u00e9miai \u00f6sszetev\u0151ivel kapcsolatban, de a lipidcs\u00f6kkent\u0151 hat\u00f3anyagok keres\u00e9s\u00e9re hagyom\u00e1nyos k\u00e9miai elv\u00e1laszt\u00e1si m\u00f3dszereket haszn\u00e1ltak, amelyek h\u00e1tr\u00e1nyai k\u00f6z\u00e9 tartozik az id\u0151ig\u00e9nyess\u00e9g, a bonyolult m\u0171velet \u00e9s a hat\u00f3anyagok elveszt\u00e9se.
\nA v\u00e9konyr\u00e9teg-kromatogr\u00e1fi\u00e1s biolumineszcencia olyan gy\u00f3gyszer-sz\u0171r\u00e9si technika, amely integr\u00e1lja az azonos\u00edt\u00e1st, az elv\u00e1laszt\u00e1st \u00e9s az aktivit\u00e1s meghat\u00e1roz\u00e1s\u00e1t. Az elm\u00falt 20 \u00e9vben sz\u00e9les k\u00f6rben alkalmazt\u00e1k antioxid\u00e1nsok, kolin\u00e9szter\u00e1z-inhibitorok \u00e9s lip\u00e1z-inhibitorok gyors sz\u0171r\u00e9s\u00e9re. Ugyanakkor ennek a m\u00f3dszernek az egyszer\u0171 m\u0171k\u00f6d\u00e9s \u00e9s az alacsony k\u00eds\u00e9rleti k\u00f6lts\u00e9g a jellemz\u0151je. A k\u00f6zvetlen ioniz\u00e1ci\u00f3s technol\u00f3gia egy \u00faj ioniz\u00e1ci\u00f3s technik\u00e1ra utal, amelyet komplex m\u00e1trix\u00fa mint\u00e1k gyors t\u00f6megspektrometri\u00e1s elemz\u00e9s\u00e9re haszn\u00e1lnak el\u0151kezel\u00e9s n\u00e9lk\u00fcl, l\u00e9gk\u00f6ri nyom\u00e1son. Az elektrosztatikus mez\u0151 induk\u00e1lta permetioniz\u00e1ci\u00f3s t\u00f6bbl\u00e9pcs\u0151s t\u00f6megspektrometri\u00e1val (TLC-EFISI-MSn) p\u00e1ros\u00edtott v\u00e9konyr\u00e9teg-kromatogr\u00e1fia megval\u00f3s\u00edthatja az akt\u00edv komponensek in-situ szerkezeti jellemz\u00e9s\u00e9t a Lycium barbarum levelek v\u00e9konyr\u00e9teg-autoradiogr\u00e1fi\u00e1j\u00e1ban. Ez a k\u00f6zvetlen ioniz\u00e1ci\u00f3s technol\u00f3gia a v\u00e9konyr\u00e9teg-kromatogr\u00e1fi\u00e1s lemezeken l\u00e9v\u0151 vegy\u00fcleteket mikrocseppes extrakci\u00f3val, tov\u00e1bbi g\u00e1z- \u00e9s f\u0171t\u0151berendez\u00e9sek n\u00e9lk\u00fcl k\u00e9pes kivonni, \u00e9s k\u00f6zvetlen\u00fcl ioniz\u00e1lni \u0151ket egy elektrosztatikus mez\u0151 hat\u00e1s\u00e1ra, miel\u0151tt elemz\u00e9s c\u00e9lj\u00e1b\u00f3l bel\u00e9pn\u00e9nek a t\u00f6megspektrom\u00e9terbe; Ez egy olyan ioniz\u00e1ci\u00f3s m\u00f3dszer, amely k\u00f6nnyen sz\u00e9tszerelhet\u0151, m\u0171k\u00f6dtethet\u0151 \u00e9s gyorsan detekt\u00e1lhat\u00f3. Ez\u00e9rt ez a cikk a v\u00e9konyr\u00e9teg-kromatogr\u00e1fi\u00e1s biol\u00f3giai autoradiogr\u00e1fi\u00e1s t\u00f6megspektrometria m\u00f3dszer\u00e9t alkalmazza a Ligustrum lucidum leveleib\u0151l sz\u00e1rmaz\u00f3 lipidcs\u00f6kkent\u0151 hat\u00e1s\u00fa komponensek gyors azonos\u00edt\u00e1s\u00e1ra, ami alapot ny\u00fajt a Ligustrum lucidum leveleinek lipidcs\u00f6kkent\u0151 hat\u00e1s\u00e1nak anyagalapj\u00e1nak tiszt\u00e1z\u00e1s\u00e1hoz.<\/p>\n

\"\"\"\"\"\"\"\"\"\"\"\"
\nEz a cikk el\u0151sz\u00f6r a Ligustrum lucidum levelek vizsg\u00e1lati mint\u00e1j\u00e1t fejlesztette ki k\u00e9t rendszerben: kloroform-etil-acet\u00e1t-metanol-v\u00edz (1:3:1:0,3, V\/V\/V\/V\/V) \u00e9s etil-acet\u00e1t-metanol-v\u00edz (6:2:1, V\/V\/V\/V), hogy t\u00f6bb foltot kapjunk. V\u00e9konyr\u00e9teg-kromatogr\u00e1fi\u00e1s lip\u00e1zg\u00e1tl\u00f3 biolumineszcencia-vizsg\u00e1lattal 10 foltot sz\u0171rt\u00fcnk ki a Ligustrum lucidum levelekben lip\u00e1zg\u00e1tl\u00f3 aktivit\u00e1ssal, majd a TLC EFISI MSn technol\u00f3gi\u00e1t haszn\u00e1ltuk a fenti foltok gyors szerkezeti elemz\u00e9s\u00e9hez. A t\u00f6bbl\u00e9pcs\u0151s t\u00f6megspektrometri\u00e1s adatok alapj\u00e1n, irodalmi jelent\u00e9sekkel kombin\u00e1lva, a k\u00fcl\u00f6nb\u00f6z\u0151 vegy\u00fcletek lehets\u00e9ges fragment\u00e1ci\u00f3s \u00fatjait felt\u00e9telezt\u00e9k, \u00e9s 15 lehets\u00e9ges szerkezetet el\u0151zetesen jellemeztek, amelyek mindegyike triterpenoid szaponin, bele\u00e9rtve 9 arbutin-titerpenoidot, 1 olean\u00e1n-titerpenoidot \u00e9s 5 18,19-gy\u0171r\u0171s triterpenoidot. A referenciaanyaggal val\u00f3 \u00f6sszehasonl\u00edt\u00e1s ut\u00e1n 12 vegy\u00fcletet valid\u00e1ltak. Ezzel a m\u00f3dszerrel gyorsan sz\u0171rhet\u0151k \u00e9s jellemezhet\u0151k a Cornus officinalis leveleib\u0151l sz\u00e1rmaz\u00f3 lip\u00e1zg\u00e1tl\u00f3 hat\u00e1s\u00fa komponensek, ami alapot ny\u00fajt a Cornus officinalis leveleinek lipidcs\u00f6kkent\u0151 hat\u00e9konys\u00e1ga alapj\u00e1nak tiszt\u00e1z\u00e1s\u00e1hoz.<\/p>","protected":false},"excerpt":{"rendered":"

Characterization of Lipase Inhibiting Active Components in Gougu Leaves by Thin Layer Chromatography Bioautography MSn Method Ilex cornuta Lindl Ex Paxt is a plant of the genus Ilex in the family Ilex, widely distributed in China. The dried leaves of Gougu, a traditional Chinese medicine, have been included in many herbal monographs since the Tang […]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"\nCharacterization of Lipase Inhibiting Active Components in Gougu Leaves by Thin Layer Chromatography Bioautography MSn Method - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/hu\/gougu-levelek\/\" \/>\n<meta property=\"og:locale\" content=\"hu_HU\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Characterization of Lipase Inhibiting Active Components in Gougu Leaves by Thin Layer Chromatography Bioautography MSn Method - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"Characterization of Lipase Inhibiting Active Components in Gougu Leaves by Thin Layer Chromatography Bioautography MSn Method Ilex cornuta Lindl Ex Paxt is a plant of the genus Ilex in the family Ilex, widely distributed in China. 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