{"id":7760,"date":"2024-08-08T11:06:18","date_gmt":"2024-08-08T11:06:18","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=7760"},"modified":"2024-08-08T11:06:18","modified_gmt":"2024-08-08T11:06:18","slug":"amylase","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/hu\/amylase\/","title":{"rendered":"Mi a k\u00fcl\u00f6nbs\u00e9g az alfa-amil\u00e1z, a b\u00e9ta-amil\u00e1z \u00e9s a glikozil\u00e1lt amil\u00e1z k\u00f6z\u00f6tt?"},"content":{"rendered":"<p>Szerepe szerint az \u03b1-amil\u00e1z, \u03b2-amil\u00e1z \u00e9s szacharifik\u00e1ci\u00f3s amil\u00e1z diagram k\u00f6nnyen hagyja, hogy az emberek t\u00e9vesen azt gondolj\u00e1k, hogy a szerepe a h\u00e1rom szubsztr\u00e1t kell lennie a kem\u00e9ny\u00edt\u0151, dextrin \u00e9s a malt\u00f3z, illetve, vannak gyerekek egyszer megk\u00e9rdezte nekem szacharifik\u00e1ci\u00f3s amil\u00e1z nem malt\u00f3z enzim. Ez\u00e9rt \u00fagy gondolom, hogy sz\u00fcks\u00e9ges egy kis elemz\u00e9st v\u00e9gezni a h\u00e1rom szerep\u00e9r\u0151l.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-7761\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-331.png\" alt=\"\" width=\"438\" height=\"55\" \/><\/p>\n<p>A k\u00fcl\u00f6nb\u00f6z\u0151 t\u00edpus\u00fa amil\u00e1zok szerep\u00e9nek meg\u00e9rt\u00e9s\u00e9nek megk\u00f6nny\u00edt\u00e9se \u00e9rdek\u00e9ben el\u0151sz\u00f6r k\u00e9t fogalomcsoportot kell azonos\u00edtani: <strong>egyenes l\u00e1nc\u00fa versus el\u00e1gaz\u00f3 kem\u00e9ny\u00edt\u0151, valamint \u03b1-1,4-glikozidos \u00e9s \u03b1-1,6-glikozidos k\u00f6t\u00e9sek.<\/strong><\/p>\n<p>A kem\u00e9ny\u00edt\u0151nek k\u00e9t t\u00edpusa van: az egyenes l\u00e1nc\u00fa \u00e9s az el\u00e1gaz\u00f3 l\u00e1nc\u00fa kem\u00e9ny\u00edt\u0151. Az egyenes l\u00e1nc\u00fa amilopektin el\u00e1gaz\u00e1s n\u00e9lk\u00fcli, spir\u00e1lis szerkezet\u0171, amelyet csak a fej\u00e9t\u0151l a v\u00e9g\u00e9ig \u03b1-1,4-glikozidos k\u00f6t\u00e9sekkel \u00f6sszekapcsolt gl\u00fck\u00f3zmaradv\u00e1nyok alkotnak; az el\u00e1gaz\u00f3 l\u00e1nc\u00fa amilopektint a gl\u00fck\u00f3zmaradv\u00e1nyok alkotj\u00e1k, amelyek minden l\u00e1ncban fej\u00e9t\u0151l a v\u00e9g\u00e9ig \u03b1-1,4-glikozidos k\u00f6t\u00e9sekkel kapcsol\u00f3dnak, de az el\u00e1gaz\u00e1sokn\u00e1l \u03b1-1,6-glikozidos k\u00f6t\u00e9sekkel.<\/p>\n<p><strong>\u03b1-Amil\u00e1z \u00e9s szerepe<\/strong><\/p>\n<p>Az \u03b1-amil\u00e1z, m\u00e1s n\u00e9ven endoamil\u00e1z, egy metallohidrol\u00e1z, amelynek aktivit\u00e1s\u00e1hoz kalciumionokra van sz\u00fcks\u00e9ge, amelyek az enzimfeh\u00e9rj\u00e9hez k\u00f6t\u0151dnek, \u00e9s amelyek a kel\u00e1tk\u00e9pz\u0151 szerrel (EDTA) t\u00f6rt\u00e9n\u0151 kezel\u00e9ssel elvesz\u00edthet\u0151k.<\/p>\n<p><strong>Az \u03b1-amil\u00e1z k\u00e9pes v\u00e9letlenszer\u0171en hidroliz\u00e1lni a kem\u00e9ny\u00edt\u0151n bel\u00fcli \u03b1-1,4-glikozidos k\u00f6t\u00e9st, de nem k\u00e9pes hidroliz\u00e1lni az \u03b1-1,6-glikozidos k\u00f6t\u00e9st.<\/strong><\/p>\n<p><strong>Ha a szubsztr\u00e1t egyenes l\u00e1nc\u00fa kem\u00e9ny\u00edt\u0151, a hidrol\u00edzis sor\u00e1n gl\u00fck\u00f3z, malt\u00f3z \u00e9s maltotri\u00f3z keletkezik; ha a hat\u00f3 szubsztr\u00e1t el\u00e1gaz\u00f3 l\u00e1nc\u00fa kem\u00e9ny\u00edt\u0151, a hidrol\u00edzis term\u00e9kei gl\u00fck\u00f3z, malt\u00f3z, maltotri\u00f3z \u00e9s \u03b1-dextrin, amely h\u00e1romn\u00e1l t\u00f6bb gl\u00fck\u00f3zmarad\u00e9kot tartalmaz\u00f3 \u03b1-1,6-glikozidos k\u00f6t\u00e9seket tartalmaz.<\/strong><\/p>\n<p>Azt is meg kell jegyezni, hogy az \u03b1 \u00e9s \u03b2 az \u03b1-amil\u00e1zban \u00e9s a \u03b2-amil\u00e1zban nem jelez semmilyen konform\u00e1ci\u00f3s kapcsolatot, hanem egyszer\u0171en csak sz\u00e1moz\u00e1ssal van jel\u00f6lve.<\/p>\n<p>A term\u00e9szetben az \u03b1-amil\u00e1z el\u0151\u00e1ll\u00edt\u00e1s\u00e1nak sz\u00e1mos m\u00f3dja l\u00e9tezik, \u00e9s p\u00e9ld\u00e1ul a mikrobi\u00e1lis ferment\u00e1ci\u00f3 az egyik legelterjedtebb m\u00f3dszer. Ezenk\u00edv\u00fcl ez az enzim n\u00f6v\u00e9nyekb\u0151l vagy \u00e1llatokb\u00f3l is kivonhat\u00f3, \u00e9s a k\u00fcl\u00f6nb\u00f6z\u0151 m\u00f3don nyert amil\u00e1z tulajdons\u00e1gai elt\u00e9r\u0151ek. Az ipari gy\u00e1rt\u00e1si folyamatban az \u03b1-amil\u00e1z ir\u00e1nti nagy ig\u00e9ny miatt \u00e1ltal\u00e1ban gomb\u00e1k \u00e9s bakt\u00e9riumok ferment\u00e1ci\u00f3j\u00e1val \u00e1ll\u00edtj\u00e1k el\u0151. A Bacillus subtilis, a Bacillus sphaericus \u00e9s a Streptomyces sphaericus k\u00e9pes \u03b1-amil\u00e1z el\u0151\u00e1ll\u00edt\u00e1s\u00e1ra.<\/p>\n<p><strong>\u03b2-Amil\u00e1z \u00e9s funkci\u00f3ja<\/strong><\/p>\n<p>A \u03b2-amil\u00e1z, m\u00e1s n\u00e9ven exoamil\u00e1z egy szulfhidrilcsoportokat tartalmaz\u00f3 hidrol\u00e1z. Az enzim a kem\u00e9ny\u00edt\u0151 nem reduk\u00e1l\u00f3 v\u00e9g\u00e9r\u0151l indul, \u00e9s az \u03b1-1,4-glikozidos k\u00f6t\u00e9st egym\u00e1s ut\u00e1n k\u00e9t gl\u00fck\u00f3zmaradv\u00e1nnyal hidroliz\u00e1lja, \u00edgy malt\u00f3z keletkezik, amely nem tudja hidroliz\u00e1lni az \u03b1-1,6-glikozidos k\u00f6t\u00e9st, \u00e9s az el\u00e1gaz\u00e1si pontot sem tudja \u00e1tl\u00e9pni, \u00edgy egy nagyon hossz\u00fa dextrin, azaz \u03b2-dextrin marad.<\/p>\n<p><strong>Ez\u00e9rt amikor a \u03b2-amil\u00e1z egyenes l\u00e1nc\u00fa kem\u00e9ny\u00edt\u0151kre hat, szinte kiz\u00e1r\u00f3lag malt\u00f3zt termel; amikor el\u00e1gaz\u00f3 l\u00e1nc\u00fa kem\u00e9ny\u00edt\u0151kre alkalmazz\u00e1k, a term\u00e9kek malt\u00f3z \u00e9s \u03b2-dextrin.<\/strong><\/p>\n<p><strong>R-enzim \u00e9s hat\u00e1sa<\/strong><\/p>\n<p>Az R-enzimek, m\u00e1s n\u00e9ven debranch\u00e1l\u00f3 enzimek, az \u03b1-1,6-glikozidos k\u00f6t\u00e9sre hatnak. A debranching enzimek jelenl\u00e9t\u00e9ben az \u03b1-dextrin \u00e9s \u03b2-dextrin fajok \u03b1-1,6-glikozidos k\u00f6t\u00e9se hidroliz\u00e1l\u00f3dik, \u00e9s az el\u00e1gaz\u00f3 el\u00e1gaz\u00f3 l\u00e1ncok elt\u00e1vol\u00edt\u00e1sra ker\u00fclnek.<\/p>\n<p>A fennmarad\u00f3 egyenes l\u00e1ncokat az \u03b1-amil\u00e1z \u00e9s a \u03b2-amil\u00e1z hidroliz\u00e1lja, \u00edgy malt\u00f3z \u00e9s gl\u00fck\u00f3z keletkezik. Az R-amil\u00e1zok azonban nem k\u00e9pesek k\u00f6zvetlen\u00fcl hidroliz\u00e1lni az \u03b1-1,6-glikozidos k\u00f6t\u00e9seket az el\u00e1gaz\u00f3 kem\u00e9ny\u00edt\u0151kben.<\/p>\n<p><strong>Glikozil\u00e1l\u00f3 amil\u00e1z \u00e9s hat\u00e1sa<\/strong><\/p>\n<p>A glikozil\u00e1l\u00f3 amil\u00e1z vagy gl\u00fckoamil\u00e1z olyan enzim, amely a kem\u00e9ny\u00edt\u0151t gl\u00fck\u00f3zz\u00e1 hidroliz\u00e1lja, ahol a hidrol\u00edzis sz\u00e1zal\u00e9kos ar\u00e1nya el\u00e9rheti az 100%-t, \u00e9s gyakran haszn\u00e1lj\u00e1k a kem\u00e9ny\u00edt\u0151 szaharos\u00edt\u00f3k\u00e9nt. A gl\u00fckoamil\u00e1z nem t\u00fal specifikus \u00e9s sok anyagra alkalmazhat\u00f3, nemcsak a kem\u00e9ny\u00edt\u0151molekula nem reduk\u00e1l\u00f3 v\u00e9g\u00e9r\u0151l k\u00e9pes elv\u00e1gni az \u03b1-1,4 glikozidos k\u00f6t\u00e9st, hanem az \u03b1-1,6 glikozidos k\u00f6t\u00e9st is, csakhogy az \u03b1-1,4 glikozidos k\u00f6t\u00e9s hidrol\u00edzise kicsit gyorsabb. Az is lehet <strong>hidroliz\u00e1lja a dextrint, a malt\u00f3zt \u00e9s a glikog\u00e9nt<\/strong>. A hidrol\u00edzis a szubsztr\u00e1tmolekula v\u00e9g\u00e9r\u0151l indul, \u00e9s exonukle\u00e1z.<\/p>\n<p>Az \u03b1-amil\u00e1z, a \u03b2-amil\u00e1z \u00e9s a glikozil\u00e1lt amil\u00e1z el\u00e1gaz\u00f3 l\u00e1nc\u00fa kem\u00e9ny\u00edt\u0151re gyakorolt hat\u00e1s\u00e1nak eredm\u00e9nyei a k\u00f6vetkez\u0151 \u00e1br\u00e1n \u00e1br\u00e1zolhat\u00f3k:<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-7762\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/2-33.jpg\" alt=\"\" width=\"534\" height=\"309\" \/><\/p>","protected":false},"excerpt":{"rendered":"<p>According to the role of \u03b1-amylase, \u03b2-amylase and saccharification amylase diagram is easy to let people mistakenly think that the role of the three substrates must be starch, dextrin and maltose, respectively, there are children once asked me saccharification amylase is not maltose enzyme. Therefore, I think it is necessary to do a little analysis [&hellip;]<\/p>","protected":false},"author":4,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[46],"tags":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.0 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>What is the difference between alpha-amylase, beta-amylase, and glycosylated amylase? - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/hu\/amylase\/\" \/>\n<meta property=\"og:locale\" content=\"hu_HU\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"What is the difference between alpha-amylase, beta-amylase, and glycosylated amylase? - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"According to the role of \u03b1-amylase, \u03b2-amylase and saccharification amylase diagram is easy to let people mistakenly think that the role of the three substrates must be starch, dextrin and maltose, respectively, there are children once asked me saccharification amylase is not maltose enzyme. 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