{"id":7543,"date":"2024-08-06T20:24:12","date_gmt":"2024-08-06T20:24:12","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=7543"},"modified":"2024-08-06T20:24:12","modified_gmt":"2024-08-06T20:24:12","slug":"mulberry-branches","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/hu\/mulberry-branches\/","title":{"rendered":"\u00d6t flavonoid komponens egyidej\u0171 meghat\u00e1roz\u00e1sa k\u00fcl\u00f6nb\u00f6z\u0151 t\u00edpus\u00fa eperfa \u00e1gakban UPLC m\u00f3dszerrel"},"content":{"rendered":"

\u00d6t flavonoid komponens egyidej\u0171 meghat\u00e1roz\u00e1sa k\u00fcl\u00f6nb\u00f6z\u0151 t\u00edpus\u00fa eperfa \u00e1gakban UPLC m\u00f3dszerrel
\nAz eperfa \u00e1gai, m\u00e1s n\u00e9ven eperfacs\u00edkok, az eperfaf\u00e9l\u00e9k csal\u00e1dj\u00e1ba tartoz\u00f3 Morus alba L. \u00e9s Morus alba M. n\u00f6v\u00e9nyek Australis Poir. \u00e9s Huasan M Sz\u00e1raz zsenge \u00e1gai cathayana Hemsl. Az eperfa \u00e1gak lapos jelleg\u0171ek, enyh\u00e9n keserny\u00e9s \u00edz\u0171ek, \u00e9s a m\u00e1jmeridi\u00e1nhoz tartoznak. Hagyom\u00e1nyos k\u00ednai gy\u00f3gyn\u00f6v\u00e9ny, amely a sz\u00e9l \u00e9s a nedvess\u00e9g el\u0171z\u00e9s\u00e9vel, az \u00edz\u00fcletek eg\u00e9szs\u00e9g\u00e9nek el\u0151seg\u00edt\u00e9s\u00e9vel hat, \u00e9s els\u0151sorban olyan t\u00fcnetek kezel\u00e9s\u00e9re haszn\u00e1lj\u00e1k, mint a reuma, a b\u00e9nul\u00e1s, valamint a karok \u00e9s v\u00e1llak \u00edz\u00fcleti zsibbad\u00e1sa \u00e9s f\u00e1jdalma. Az \u0151si k\u00f6nyvek feljegyezt\u00e9k, hogy az eperfa \u00e1gak \"kezelik a sz\u00e1jsz\u00e1razs\u00e1got, t\u00e1pl\u00e1lj\u00e1k a testnedveket \u00e9s t\u00e1pl\u00e1lj\u00e1k a vesevizet\", \u00e9s ter\u00e1pi\u00e1s hat\u00e1suk a nedvess\u00e9g kisz\u00e1r\u00edt\u00e1sa, a v\u00e9rkering\u00e9s \u00e9s a meridi\u00e1nok kering\u00e9s\u00e9nek el\u0151seg\u00edt\u00e9se. K\u00edn\u00e1ban a hagyom\u00e1nyos k\u00ednai orvosl\u00e1sban r\u00e9g\u00f3ta alkalmazz\u00e1k klinikai alkalmaz\u00e1sukat. Az eperfa \u00e1gak k\u00fcl\u00f6nb\u00f6z\u0151 hat\u00e9kony hat\u00f3anyagokat tartalmaznak, mint p\u00e9ld\u00e1ul flavonoidokat, alkaloidokat, aminosavakat stb. A modern farmakol\u00f3giai kutat\u00e1sok szerint az eperfa \u00e1gaknak t\u00f6bbf\u00e9le hat\u00e1suk van, t\u00f6bbek k\u00f6z\u00f6tt gyullad\u00e1scs\u00f6kkent\u0151, antioxid\u00e1ns, hipoglik\u00e9mi\u00e1s, lipidcs\u00f6kkent\u0151, v\u00e9rnyom\u00e1scs\u00f6kkent\u0151, antibakteri\u00e1lis, v\u00edrusellenes \u00e9s daganatellenes. Az eperfa \u00e1g flavonoidjai j\u00f3 hat\u00e1ssal vannak a v\u00e9rzs\u00edrok cs\u00f6kkent\u00e9s\u00e9re, a v\u00e9rcukorszint cs\u00f6kkent\u00e9s\u00e9re, valamint antibakteri\u00e1lis \u00e9s antioxid\u00e1ns tulajdons\u00e1gokkal rendelkeznek.
\nEbben a tanulm\u00e1nyban k\u00eds\u00e9rleti mintak\u00e9nt 18 t\u00e9tel eperfa \u00e1gat haszn\u00e1ltak fel, amelyek 9 k\u00fcl\u00f6nb\u00f6z\u0151 termel\u00e9si ter\u00fcletr\u0151l \u00e9s 3 k\u00fcl\u00f6nb\u00f6z\u0151 t\u00edpusb\u00f3l sz\u00e1rmaznak Chongqingb\u00f3l. Ultrahangos extrakci\u00f3t v\u00e9gezt\u00fcnk, \u00e9s ultrateljes\u00edtm\u00e9ny\u0171 folyad\u00e9kkromatogr\u00e1fi\u00e1t (UPLC) haszn\u00e1ltunk \u00f6t flavonoid, k\u00f6zt\u00fck a rutin, az eperfa pigment, a kaempferol, az izoquercitrin \u00e9s az eperfa kvercetin tartalm\u00e1nak meghat\u00e1roz\u00e1s\u00e1ra \u00e9s elemz\u00e9s\u00e9re ebben a 18 t\u00e9telben, 9 k\u00fcl\u00f6nb\u00f6z\u0151 termel\u00e9si ter\u00fcletr\u0151l \u00e9s 3 k\u00fcl\u00f6nb\u00f6z\u0151 t\u00edpusb\u00f3l sz\u00e1rmaz\u00f3 eperfa \u00e1gakban. \u00d6sszehasonl\u00edtottuk a flavonoid-tartalom k\u00fcl\u00f6nbs\u00e9geit a 18 t\u00e9tel eperfa-\u00e1g k\u00f6z\u00f6tt, ami adatt\u00e1mogat\u00e1st \u00e9s hivatkoz\u00e1si alapot szolg\u00e1ltatott a k\u00e9s\u0151bbi eperfa-\u00e1gak kutat\u00e1s\u00e1hoz \u00e9s fejleszt\u00e9s\u00e9hez.<\/p>\n

\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"
\nAz extrakci\u00f3s felt\u00e9telek kiv\u00e1laszt\u00e1sa. Ez a tanulm\u00e1ny a metanolos f\u0171t\u00e9si reflux extrakci\u00f3s m\u00f3dszert, a metanolos ultrahangos extrakci\u00f3s m\u00f3dszert, az etanolos f\u0171t\u00e9si reflux extrakci\u00f3s m\u00f3dszert \u00e9s az etanolos ultrahangos extrakci\u00f3s m\u00f3dszert vizsg\u00e1lta. Meg\u00e1llap\u00edt\u00e1st nyert, hogy a metanol mint extrakci\u00f3s old\u00f3szer haszn\u00e1lata jobb eredm\u00e9nyeket hozott. A f\u0171t\u00e9si reflux extrakci\u00f3s m\u00f3dszer \u00e9s az ultrahangos extrakci\u00f3s m\u00f3dszer extrakci\u00f3s hat\u00e1sai nem k\u00fcl\u00f6nb\u00f6ztek jelent\u0151sen. Tekintettel az ultrahangos extrakci\u00f3s m\u00f3dszer egyszer\u0171 m\u0171k\u00f6d\u00e9s\u00e9re, ebben a tanulm\u00e1nyban a metanolos ultrahangos extrakci\u00f3s m\u00f3dszert v\u00e1lasztott\u00e1k. Ez a tanulm\u00e1ny egyidej\u0171leg figyelembe vette az extrakci\u00f3s h\u0151m\u00e9rs\u00e9klet, az extrakci\u00f3s id\u0151 \u00e9s az extrakci\u00f3s id\u0151k hat\u00e1s\u00e1t az extrakci\u00f3s hat\u00e9konys\u00e1gra. A v\u00e9gs\u0151 k\u00eds\u00e9rleti eredm\u00e9nyek azt mutatt\u00e1k, hogy az \u00e1ltal\u00e1nos extrakci\u00f3s hat\u00e1s optim\u00e1lis volt, amikor az extrakci\u00f3s h\u0151m\u00e9rs\u00e9klet 25 \u2103 volt, az extrakci\u00f3s id\u0151 30 perc volt, \u00e9s az extrakci\u00f3s id\u0151k 2-szer voltak. Ez\u00e9rt a k\u00eds\u00e9rlet v\u00e9gs\u0151 extrakci\u00f3s k\u00f6r\u00fclm\u00e9nyeit \u00fagy hat\u00e1roztuk meg, hogy metanolos oldatot \u00e9s ultrahangos extrakci\u00f3s m\u00f3dszert haszn\u00e1lunk, az extrakci\u00f3s h\u0151m\u00e9rs\u00e9klet 25 \u2103, az extrakci\u00f3s id\u0151 30 perc \u00e9s az extrakci\u00f3s id\u0151 2-szeres.
\nA mobil f\u00e1zis kiv\u00e1laszt\u00e1sa. Ez a vizsg\u00e1lat n\u00e9gy mobilf\u00e1zis-\u00f6sszet\u00e9tel (0,2% hangyasavoldat+metanol, 0,2% hangyasavoldat+acetonitril, 0,2% foszforsavoldat+metanol, 0,2% foszforsavoldat+acetonitril) cs\u00facsk\u00e9pz\u0151d\u00e9sre gyakorolt hat\u00e1s\u00e1nak k\u00fcl\u00f6nbs\u00e9geit vizsg\u00e1lta. A k\u00eds\u00e9rleti eredm\u00e9nyek azt mutatt\u00e1k, hogy amikor a mozg\u00f3f\u00e1zis 0,2% hangyasavoldat+metanol \u00e9s 0,2% foszforsavoldat+metanol volt, a cs\u00facsk\u00e9pz\u0151d\u00e9s ideje k\u00e9s\u0151bb \u00e9s az elemz\u00e9si id\u0151 hosszabb volt, k\u00fcl\u00f6n\u00f6sen a kvercetin eset\u00e9ben. Amikor a mozg\u00f3f\u00e1zis metanol volt, a cs\u00facsk\u00e9pz\u0151d\u00e9si id\u0151 k\u00e9s\u0151bb k\u00f6vetkezett be, ami befoly\u00e1solta a teljes elemz\u00e9si id\u0151t. Amikor a mozg\u00f3f\u00e1zis 0,2% foszforsavoldat+acetonitril volt, az elemz\u00e9si id\u0151 r\u00f6videbb volt, a cs\u00facs alakja \u00e9s az elv\u00e1laszt\u00e1si hat\u00e1s jobb volt. Ez\u00e9rt ebben a tanulm\u00e1nyban a 0,2% foszforsavoldat+acetonitrilt v\u00e1lasztottuk mobilf\u00e1zisnak. Ugyanakkor a k\u00fcl\u00f6nb\u00f6z\u0151 oszloph\u0151m\u00e9rs\u00e9kletek (30, 35, 40 \u2103) hat\u00e1s\u00e1t is megvizsg\u00e1ltuk a k\u00eds\u00e9rleti eredm\u00e9nyekre. A k\u00eds\u00e9rleti eredm\u00e9nyek azt mutatt\u00e1k, hogy az elv\u00e1laszt\u00e1si hat\u00e1s akkor volt optim\u00e1lis, amikor az oszlop h\u0151m\u00e9rs\u00e9klete 35 \u2103 volt.
\nAz eperfa \u00e1gak flavonoidtartalma. A kutat\u00e1si eredm\u00e9nyek azt mutatt\u00e1k, hogy a rutin, az izoquercitrin, az eperfa pigment, a kaempferol \u00e9s a kaempferol mind a 18 t\u00e9tel eperfa\u00e1g-mint\u00e1ban kimutathat\u00f3 volt, de a tartalmukban jelent\u0151s k\u00fcl\u00f6nbs\u00e9gek voltak. A legmagasabb kaempferol-tartalm\u00fa \u00e1tlagtartalom 135,154 \u03bc g\/g, a legalacsonyabb kaempferol-tartalm\u00fa \u00e1tlagtartalom 6,929 \u03bc g\/g volt. Ez arra utal, hogy az eperfa \u00e1gak (k\u00fcl\u00f6n\u00f6sen a Huangjueya v\u00e1rosban, Nan'an k\u00f6rzetben tal\u00e1lhat\u00f3 Huasang) forr\u00e1sanyagk\u00e9nt haszn\u00e1lhat\u00f3k a kaempferol kivon\u00e1s\u00e1ra, valamint tov\u00e1bbi fejleszt\u00e9s\u00e9re \u00e9s felhaszn\u00e1l\u00e1s\u00e1ra. Az eperfa \u00e1gak ezen \u00f6t flavonoid tartalm\u00e1t k\u00fcl\u00f6nb\u00f6z\u0151 t\u00e9nyez\u0151k befoly\u00e1solj\u00e1k, mint p\u00e9ld\u00e1ul az \u00e1gak betakar\u00edt\u00e1si ideje, az eperfa t\u00edpusa, az \u00e1gak r\u00e9szei (el\u00fcls\u0151, k\u00f6z\u00e9ps\u0151 \u00e9s h\u00e1ts\u00f3), az \u00e1gak eredete stb. A Sangxinsu AIDS-v\u00edrus \u00e9s daganatellenes hat\u00e1ssal rendelkezik. Az eperfa pigmentek \u00e9s az izoquercitrin egyar\u00e1nt rendelkeznek antioxid\u00e1ns aktivit\u00e1ssal \u00e9s k\u00e9pesek a DPPH szabadgy\u00f6k-aktivit\u00e1s elnyel\u00e9s\u00e9re. A rutin fokozhatja a kapill\u00e1risok rugalmass\u00e1g\u00e1t, v\u00e9dheti a k\u00fcl\u00f6nb\u00f6z\u0151 sz\u00f6veteket \u00e9s szerveket, \u00e9s kiv\u00e1lthatja a sejtek apopt\u00f3zis\u00e1t. A kaempferol j\u00f3 g\u00e1tl\u00f3 hat\u00e1ssal van a r\u00e1kos sejtekre. A rutinhoz hasonl\u00f3an apopt\u00f3zist induk\u00e1l a sejthal\u00e1l folyamata sor\u00e1n, \u00e9s r\u00e1kellenes hat\u00e1s\u00fa. \u00d6sszefoglalva, az eperfa kivonat, az eperfa pigment, az izoquercitrin, a rutin \u00e9s a kaempferol mind j\u00f3 gy\u00f3gy\u00e1szati \u00e9rt\u00e9kkel b\u00edrnak, \u00e9s nagy jelent\u0151s\u00e9ggel b\u00edrnak a klinikai gy\u00f3gy\u00e1szatban. Az eperfa-forr\u00e1sok sz\u00e9les \u00fcltet\u00e9si ter\u00fclettel rendelkeznek a H\u00e1rom-szurdok-t\u00e1roz\u00f3 ter\u00fclet\u00e9n, k\u00f6nnyen beszerezhet\u0151k \u00e9s alacsony nyersanyagbeszerz\u00e9si k\u00f6lts\u00e9gekkel rendelkeznek. Az eperfa \u00e1gak nyersanyagk\u00e9nt val\u00f3 felhaszn\u00e1l\u00e1sa a flavonoidok, p\u00e9ld\u00e1ul a kvercetin, az eperfa pigment, az izoquercitrin, a rutin \u00e9s a kaempferol kivon\u00e1s\u00e1ra az eperfa \u00e1gak tov\u00e1bbi fejleszt\u00e9s\u00e9nek \u00e9s felhaszn\u00e1l\u00e1s\u00e1nak \u00faj trendje lesz.<\/p>","protected":false},"excerpt":{"rendered":"

\u00d6t flavonoid komponens egyidej\u0171 meghat\u00e1roz\u00e1sa k\u00fcl\u00f6nb\u00f6z\u0151 t\u00edpus\u00fa eperfa\u00e1gakban UPLC m\u00f3dszerrel Az eperfa\u00e1gak, m\u00e1s n\u00e9ven eperfacs\u00edkok, az eperfaf\u00e9l\u00e9k csal\u00e1dj\u00e1ba tartoz\u00f3 Morus alba L. \u00e9s Morus alba M. n\u00f6v\u00e9nyek Australis Poir. \u00e9s Huasan M Sz\u00e1raz zsenge \u00e1gai cathayana Hemsl. Az eperfa \u00e1gai lapos jelleg\u0171ek, kiss\u00e9 [...]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"\nSimultaneous determination of five flavonoid components in different types of mulberry branches using UPLC method - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/hu\/mulberry-branches\/\" \/>\n<meta property=\"og:locale\" content=\"hu_HU\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Simultaneous determination of five flavonoid components in different types of mulberry branches using UPLC method - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"Simultaneous determination of five flavonoid components in different types of mulberry branches using UPLC method Mulberry branches, also known as mulberry strips, are the Morus alba L. and Morus alba M. plants in the mulberry family Australis Poir. and Huasan M Dry tender branches of cathayana Hemsl. 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