{"id":7009,"date":"2024-08-06T18:07:01","date_gmt":"2024-08-06T18:07:01","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=7009"},"modified":"2024-08-06T18:07:01","modified_gmt":"2024-08-06T18:07:01","slug":"lotus-flowers","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/hu\/lotus-flowers\/","title":{"rendered":"A k\u00fcl\u00f6nb\u00f6z\u0151 eredet\u0171 l\u00f3tuszvir\u00e1gok min\u0151s\u00e9g\u00e9nek \u00e9rt\u00e9kel\u00e9se a k\u00e9miai mintafelismer\u0151 m\u00f3dszerrel kombin\u00e1lt ujjlenyomat-spektrum alapj\u00e1n"},"content":{"rendered":"

A k\u00fcl\u00f6nb\u00f6z\u0151 eredet\u0171 l\u00f3tuszvir\u00e1gok min\u0151s\u00e9g\u00e9nek \u00e9rt\u00e9kel\u00e9se a k\u00e9miai mintafelismer\u0151 m\u00f3dszerrel kombin\u00e1lt ujjlenyomat-spektrum alapj\u00e1n
\nAz alv\u00f3 l\u00f3tusz a Nymphaeaceae csal\u00e1dba tartoz\u00f3 \u00e9vel\u0151, v\u00edzi l\u00e1gysz\u00e1r\u00fa n\u00f6v\u00e9ny, amely Ili, Altay, Bohu \u00e9s Hszincsiang m\u00e1s ter\u00fcletein honos. Az alv\u00f3 l\u00f3tusz sz\u00e1r\u00edtott vir\u00e1gbimb\u00f3it, mint az ujgur gy\u00f3gy\u00e1szatban \u00e1ltal\u00e1nosan haszn\u00e1lt gy\u00f3gyn\u00f6v\u00e9nyt, felvett\u00e9k a K\u00ednai N\u00e9pk\u00f6zt\u00e1rsas\u00e1g Eg\u00e9szs\u00e9g\u00fcgyi Miniszt\u00e9rium\u00e1nak Gy\u00f3gyszerk\u00f6nyve ujgur gy\u00f3gyszerk\u00f6nyv\u00e9be. L\u00e1zcsillap\u00edt\u00f3, k\u00f6h\u00f6g\u00e9scsillap\u00edt\u00f3, h\u0151tiszt\u00edt\u00f3 \u00e9s m\u00e9regtelen\u00edt\u0151, nyugtat\u00f3 hat\u00e1s\u00fa, valamint fejf\u00e1j\u00e1s, l\u00e1zas k\u00f6h\u00f6g\u00e9s, gyermekekn\u00e9l a l\u00e1z okozta akut \u00e9s kr\u00f3nikus sz\u00e9lsokk kezel\u00e9s\u00e9re haszn\u00e1lhat\u00f3. Az alv\u00f3 l\u00f3tusz f\u0151k\u00e9nt flavonoidokat \u00e9s fenolos vegy\u00fcleteket tartalmaz. A modern farmakol\u00f3giai vizsg\u00e1latok kimutatt\u00e1k, hogy k\u00fcl\u00f6nb\u00f6z\u0151 biol\u00f3giai tev\u00e9kenys\u00e9gekkel rendelkezik, mint p\u00e9ld\u00e1ul antioxid\u00e1ns, gyullad\u00e1scs\u00f6kkent\u0151, hipoglik\u00e9mi\u00e1s, antivir\u00e1lis stb. A k\u00ednai gy\u00f3gyn\u00f6v\u00e9ny bels\u0151 min\u0151s\u00e9g\u00e9nek jobb ellen\u0151rz\u00e9se \u00e9rdek\u00e9ben ez a tanulm\u00e1ny a k\u00e9miai mintafelismer\u00e9si m\u00f3dszerrel kombin\u00e1lt ujjlenyomat-spektrumot k\u00edv\u00e1n haszn\u00e1lni a t\u00f6bbkomponens\u0171 tartalom meghat\u00e1roz\u00e1s\u00e1hoz, \u00e1tfog\u00f3an elemezni a k\u00ednai gy\u00f3gyn\u00f6v\u00e9nyek \u00f6sszet\u00e9tel\u00e9nek k\u00fcl\u00f6nbs\u00e9geit a k\u00fcl\u00f6nb\u00f6z\u0151 termel\u00e9si ter\u00fcleteken, tiszt\u00e1zni a termel\u00e9si ter\u00fcletek \u00e9s a jellemz\u0151 \u00f6sszetev\u0151k k\u00f6z\u00f6tti k\u00fcl\u00f6nbs\u00e9geket, referenci\u00e1t ny\u00fajtani a k\u00ednai gy\u00f3gyn\u00f6v\u00e9nyek azonos\u00edt\u00e1s\u00e1hoz \u00e9s min\u0151s\u00e9gi szabv\u00e1nyok megfogalmaz\u00e1s\u00e1hoz, \u00e9s megalapozni a tov\u00e1bbi fejleszt\u00e9st \u00e9s felhaszn\u00e1l\u00e1st.<\/p>\n

\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"<\/p>\n

Az extrakci\u00f3s old\u00f3szer kiv\u00e1laszt\u00e1sa. A Sleeping Lotus gy\u00f3gyn\u00f6v\u00e9nyben sz\u00e1mos k\u00e9miai \u00f6sszetev\u0151 \u00e9s k\u00fcl\u00f6nb\u00f6z\u0151 tulajdons\u00e1g tal\u00e1lhat\u00f3. Annak \u00e9rdek\u00e9ben, hogy a gy\u00f3gyn\u00f6v\u00e9nyben tal\u00e1lhat\u00f3 k\u00e9miai komponensek inform\u00e1ci\u00f3i jobban t\u00fckr\u00f6z\u0151djenek a kromatogramban, ez a cikk nagysz\u00e1m\u00fa szakirodalmat tanulm\u00e1nyozott, \u00e9s ebben a k\u00eds\u00e9rletben a metanol, 50% metanol \u00e9s etanol extrakci\u00f3s old\u00f3szereket v\u00e1lasztotta. Az 50% metanol \u00e9s az etanol extrakci\u00f3s old\u00f3szerk\u00e9nt val\u00f3 alkalmaz\u00e1sakor a 15 t\u00e9tel Sleeping Lotus ujjlenyomat-spektrum\u00e1nak elemz\u00e9se miatt meg\u00e1llap\u00edtott\u00e1k, hogy 273 nm hull\u00e1mhosszon 23 k\u00f6z\u00f6s cs\u00facs volt. Az 1. cs\u00facson j\u00f3l elk\u00fcl\u00f6n\u00fclt a galluszsav \u00e9s nagy volt a cs\u00facsfel\u00fclet, ez\u00e9rt ezt haszn\u00e1lt\u00e1k referenciacs\u00facsk\u00e9nt. A 350nm-en 17 k\u00f6z\u00f6s cs\u00facs tal\u00e1lhat\u00f3, amelyek k\u00f6z\u00fcl 4 komponenst azonos\u00edtottak, nevezetesen a csersavat, a t\u0171zij\u00e1t\u00e9k glikozidot, a lila felh\u0151 glikozidot \u00e9s a kvercetint. Ek\u00f6zben 15 t\u00e9telnyi minta ujjlenyomat-spektrum\u00e1nak hasonl\u00f3s\u00e1g\u00e1t \u00e9rt\u00e9kelt\u00fck, \u00e9s az eredm\u00e9nyek j\u00f3 hasonl\u00f3s\u00e1got mutattak a kontroll ujjlenyomat-spektrummal. A cs\u00facsok kis sz\u00e1ma hi\u00e1nyos extrakci\u00f3hoz vezet. A k\u00fcl\u00f6nb\u00f6z\u0151 t\u00e9nyez\u0151k \u00e1tfog\u00f3 elemz\u00e9s\u00e9vel meghat\u00e1rozt\u00e1k az optim\u00e1lis extrakci\u00f3s m\u00f3dszert, amely metanolt haszn\u00e1l old\u00f3szerk\u00e9nt.<\/p>\n

Az \u00e9rz\u00e9kel\u00e9si hull\u00e1mhossz kiv\u00e1laszt\u00e1sa. Ebben a tanulm\u00e1nyban di\u00f3dasoros detektort haszn\u00e1ltak a l\u00f3tuszvir\u00e1g ujjlenyomat-spektrum\u00e1nak teljes hull\u00e1mhossz\u00fa (190-800 nm) p\u00e1szt\u00e1z\u00e1s\u00e1hoz. A bemutatott 3D spektrum alapj\u00e1n l\u00e1that\u00f3, hogy a kromatogr\u00e1fi\u00e1s cs\u00facsok elv\u00e1laszt\u00e1sa magas, a cs\u00facs szimmetri\u00e1ja j\u00f3, az alapvonal stabil, \u00e9s a cs\u00facskapacit\u00e1s nagy a 273nm \u00e9s 350nm hull\u00e1mhossz\u00fas\u00e1g\u00fa k\u00f6r\u00fclm\u00e9nyek k\u00f6z\u00f6tt. A vizsg\u00e1lati oldat kapott spektruma gazdagabb inform\u00e1ci\u00f3t mutathat. Ez\u00e9rt a 273nm \u00e9s 350nm hull\u00e1mhosszakat v\u00e1lasztottuk ki a m\u00e9r\u00e9si hull\u00e1mhossznak.<\/p>\n

A kromatogr\u00e1fi\u00e1s felt\u00e9telek kiv\u00e1laszt\u00e1sa. Megvizsg\u00e1ltuk a metanol \u00e9s az acetonitril mint mobil f\u00e1zisok hat\u00e1s\u00e1t a l\u00f3tuszvir\u00e1gok ujjlenyomat-spektrum\u00e1ra. A metanol \u00e9s az acetonitril mozg\u00f3f\u00e1zisk\u00e9nt val\u00f3 alkalmaz\u00e1sakor izokratikus anal\u00edzis eset\u00e9n az elv\u00e1laszt\u00e1s hat\u00e9konys\u00e1ga gyenge. Az egyes kromatogr\u00e1fi\u00e1s cs\u00facsok jobb elv\u00e1laszt\u00e1s\u00e1nak el\u00e9r\u00e9se \u00e9rdek\u00e9ben gradiens el\u00faci\u00f3t v\u00e1lasztottak. A metanol-acetonitril mint mobil f\u00e1zishoz k\u00e9pest t\u00f6bb kromatogr\u00e1fi\u00e1s cs\u00facsot lehet kapni az ujjlenyomat kromatogramban. A k\u00e9t k\u00fcl\u00f6nb\u00f6z\u0151 mobilf\u00e1zisb\u00f3l kapott kromatogramok \u00f6sszehasonl\u00edt\u00e1s\u00e1val meg\u00e1llap\u00edtott\u00e1k, hogy az acetonitril haszn\u00e1lat\u00e1val az elv\u00e1laszt\u00e1si teljes\u00edtm\u00e9ny jobb, mint a metanol\u00e9val. Ez\u00e9rt ebben a vizsg\u00e1latban az acetonitrilt v\u00e1lasztottuk mobilf\u00e1zisnak.
\nAz analitikai m\u00f3dszerek \u00e9rt\u00e9kel\u00e9se. Az alv\u00f3 l\u00f3tuszvir\u00e1g 15 t\u00e9tel\u00e9nek ujjlenyomat-spektrum\u00e1t elemezve meg\u00e1llap\u00edtott\u00e1k, hogy 273 nm hull\u00e1mhosszon 23 k\u00f6z\u00f6s cs\u00facs volt. Az 1. cs\u00facson j\u00f3l elk\u00fcl\u00f6n\u00fclt a galluszsav \u00e9s nagy volt a cs\u00facsfel\u00fclet, ez\u00e9rt ezt haszn\u00e1lt\u00e1k referenciacs\u00facsk\u00e9nt. A 350nm-en 17 k\u00f6z\u00f6s cs\u00facs tal\u00e1lhat\u00f3, amelyek k\u00f6z\u00fcl 4 komponenst azonos\u00edtottak, nevezetesen a csersavat, a t\u0171zij\u00e1t\u00e9k glikozidot, a lila felh\u0151 glikozidot \u00e9s a kvercetint. Ek\u00f6zben 15 t\u00e9telnyi minta ujjlenyomat-spektrum\u00e1nak hasonl\u00f3s\u00e1g\u00e1t \u00e9rt\u00e9kelt\u00fck, \u00e9s az eredm\u00e9nyek j\u00f3 hasonl\u00f3s\u00e1got mutattak a kontroll ujjlenyomat-spektrummal. A HPLC-ujjlenyomat \u00e9s a klaszterelemz\u00e9s, a PCA, az OPLS-DA kombin\u00e1ci\u00f3ja felhaszn\u00e1lhat\u00f3 a k\u00fcl\u00f6nb\u00f6z\u0151 eredet\u0171 \u00e9s t\u00e9telekb\u0151l sz\u00e1rmaz\u00f3 gy\u00f3gy\u00e1szati anyagok klaszterez\u00e9s\u00e9hez \u00e9s komponensek megk\u00fcl\u00f6nb\u00f6ztet\u0151 elemz\u00e9s\u00e9hez. Ez a tanulm\u00e1ny k\u00e9miai mintafelismer\u00e9si m\u00f3dszereket alkalmazott a Nelumbo nucifera gy\u00f3gyn\u00f6v\u00e9ny klaszterelemz\u00e9s, PCA \u00e9s OPLS-DA oszt\u00e1lyoz\u00e1si mint\u00e1k elv\u00e9gz\u00e9s\u00e9re. A h\u00e1rom m\u00f3dszer elemz\u00e9si eredm\u00e9nyei \u00f6sszhangban voltak. A Nelumbo nucifera tizen\u00f6t t\u00e9tel\u00e9t 273 nm-en n\u00e9gy kateg\u00f3ri\u00e1ba sorolt\u00e1k, a k\u00fcl\u00f6nbs\u00e9gek els\u0151sorban a 17, 1, 4, 15, 20, 23, 3, 6, 2 \u00e9s 21-es cs\u00facsokban t\u00fckr\u00f6z\u0151dtek. Ezek k\u00f6z\u00fcl az 1. cs\u00facs a galluszsav, a 15. cs\u00facs a puerarin, a 20. cs\u00facs pedig a kvercetin volt. A 350 nm-en h\u00e1rom kateg\u00f3ri\u00e1ba sorolhat\u00f3, a k\u00fcl\u00f6nbs\u00e9gek els\u0151sorban a 14, 1, 13, 7, 8, 11, 10 \u00e9s 2 cs\u00facsok sz\u0171r\u00e9s\u00e9ben t\u00fckr\u00f6z\u0151dtek. Ezek k\u00f6z\u00fcl a 14-es cs\u00facs a kvercetin, a 7-es cs\u00facs a t\u0171zij\u00e1t\u00e9k glikozid, a 8-as cs\u00facs pedig a lila felh\u0151 glikozid. Ezek a komponensek szolg\u00e1lhatnak a k\u00fcl\u00f6nb\u00f6z\u0151 term\u0151ter\u00fcletekr\u0151l sz\u00e1rmaz\u00f3 l\u00f3tusz gy\u00f3gy\u00e1szati anyagok min\u0151s\u00e9g\u00e9nek megk\u00fcl\u00f6nb\u00f6ztet\u00e9s\u00e9re \u00e9s azonos\u00edt\u00e1s\u00e1ra szolg\u00e1l\u00f3 f\u0151 jellemz\u0151 komponensekk\u00e9nt. Kutat\u00f3csoportunk folytatja az ismeretlen differenci\u00e1lkomponensek m\u00e9lyrehat\u00f3 vizsg\u00e1lat\u00e1t, hogy \u00e1tfog\u00f3bb referenci\u00e1t ny\u00fajtson a l\u00f3tusz gy\u00f3gy\u00e1szati anyagok min\u0151s\u00e9g\u00e9nek \u00e9rt\u00e9kel\u00e9s\u00e9hez.<\/p>","protected":false},"excerpt":{"rendered":"

Evaluation of the quality of lotus flowers from different origins based on fingerprint spectrum combined with chemical pattern recognition method Sleeping lotus is a perennial aquatic herbaceous plant in the family Nymphaeaceae, native to Ili, Altay, Bohu, and other areas in Xinjiang. The dried flower buds of the Sleeping Lotus, as a commonly used medicinal […]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"\nEvaluation of the quality of lotus flowers from different origins based on fingerprint spectrum combined with chemical pattern recognition method - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/hu\/lotus-flowers\/\" \/>\n<meta property=\"og:locale\" content=\"hu_HU\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Evaluation of the quality of lotus flowers from different origins based on fingerprint spectrum combined with chemical pattern recognition method - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"Evaluation of the quality of lotus flowers from different origins based on fingerprint spectrum combined with chemical pattern recognition method Sleeping lotus is a perennial aquatic herbaceous plant in the family Nymphaeaceae, native to Ili, Altay, Bohu, and other areas in Xinjiang. 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