{"id":6879,"date":"2024-08-06T14:59:46","date_gmt":"2024-08-06T14:59:46","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=6879"},"modified":"2024-08-06T14:59:46","modified_gmt":"2024-08-06T14:59:46","slug":"turnip-neutral-polysaccharides","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/hu\/feherrepa-semleges-poliszacharidok\/","title":{"rendered":"El\u0151zetes tanulm\u00e1ny a feh\u00e9rr\u00e9pa semleges poliszacharidok v\u00e9d\u0151mechanizmus\u00e1r\u00f3l a PC12 sejtek oxidat\u00edv k\u00e1rosod\u00e1s\u00e1val szemben"},"content":{"rendered":"

El\u0151zetes tanulm\u00e1ny a feh\u00e9rr\u00e9pa semleges poliszacharidok v\u00e9d\u0151mechanizmus\u00e1r\u00f3l a PC12 sejtek oxidat\u00edv k\u00e1rosod\u00e1s\u00e1val szemben
\nA r\u00e9pa (Brassica rapa L.), m\u00e1s n\u00e9ven Chagu vagy Manjing, a Brassicaceae csal\u00e1dba \u00e9s a Brassica nemzets\u00e9gbe tartozik. Gy\u00f6ker\u00e9t gy\u00f3gyn\u00f6v\u00e9nyk\u00e9nt haszn\u00e1lj\u00e1k, \u00e9s t\u00e1panyagokban gazdag. Hossz\u00fa ideig fogyaszthat\u00f3, \u00e9s \"hossz\u00fa \u00e9let\u0171 gy\u00fcm\u00f6lcsk\u00e9nt\" ismert. \u00c9tv\u00e1gyjav\u00edt\u00f3 \u00e9s em\u00e9szt\u00e9st el\u0151seg\u00edt\u0151, a qi kering\u00e9s\u00e9t el\u0151seg\u00edt\u0151, k\u00f6h\u00f6g\u00e9st \u00e9s asztm\u00e1t enyh\u00edt\u0151, vizelethajt\u00e1st \u00e9s m\u00e9regtelen\u00edt\u00e9st el\u0151seg\u00edt\u0151 hat\u00e1sa van. A modern farmakol\u00f3giai kutat\u00e1sok meg\u00e1llap\u00edtott\u00e1k, hogy a feh\u00e9rr\u00e9pa olyan farmakol\u00f3giai tev\u00e9kenys\u00e9gekkel rendelkezik, mint a v\u00e9rcukorszint cs\u00f6kkent\u00e9se, antioxid\u00e1ci\u00f3, \u00f6reged\u00e9sg\u00e1tl\u00e1s, daganatellenes, immunit\u00e1sszab\u00e1lyoz\u00f3 \u00e9s gyullad\u00e1scs\u00f6kkent\u0151. Az el\u0151zetes kutat\u00e1s sor\u00e1n a kutat\u00f3csoport m\u00e9r\u00e9se szerint a feh\u00e9rr\u00e9pa poliszacharid tartalma k\u00f6r\u00fclbel\u00fcl 11,53% volt. Zs\u00edrtalan\u00edt\u00e1s \u00e9s sz\u00edntelen\u00edt\u0151 tiszt\u00edt\u00e1s ut\u00e1n a v\u00edzben old\u00f3d\u00f3 feh\u00e9rr\u00e9pa poliszacharid BRP-t nyert\u00e9k. Ezt tov\u00e1bb izol\u00e1lt\u00e1k \u00e9s tiszt\u00edtott\u00e1k, hogy semleges poliszacharidokat nyerjenek a Brassica rapa L.-b\u0151l (BRNP) \u00e9s savas poliszacharidokat a Brassica rapa L.-b\u0151l (BRAP), \u00e9s szerkezet\u00fcket azonos\u00edtott\u00e1k. Az el\u0151zetes k\u00eds\u00e9rletek azt mutatt\u00e1k, hogy a r\u00e9pa poliszacharidok j\u00f3 antioxid\u00e1ns aktivit\u00e1ssal rendelkeznek, ami megalapozza a r\u00e9pa poliszacharidok antioxid\u00e1ns \u00e9s \u00f6reged\u00e9sg\u00e1tl\u00f3 kutat\u00e1sokban val\u00f3 alkalmaz\u00e1s\u00e1t. Ez\u00e9rt ez a tanulm\u00e1ny H2O2 \u00e1ltal induk\u00e1lt PC12 sejtek korai \u00f6reged\u00e9si modellj\u00e9t hozta l\u00e9tre, hogy megvizsg\u00e1lja a Bax, Bcl-2 \u00e9s Caspase-3 feh\u00e9rj\u00e9k expresszi\u00f3s szintj\u00e9t PC12 sejtekben a H2O2 \u00e1ltal induk\u00e1lt s\u00e9r\u00fcl\u00e9st k\u00f6vet\u0151en BRNP-vel, \u00e9s tov\u00e1bb tiszt\u00e1zza a BRNP \u00f6reged\u00e9sg\u00e1tl\u00f3 mechanizmus\u00e1t.<\/p>\n

\"\"\"\"\"\"\"\"\"\"\"\"\"\"
\nA H2O2 egy reakt\u00edv oxig\u00e9nfajok molekula, amely n\u00f6velheti az intracellul\u00e1ris reakt\u00edv oxig\u00e9nfajok tartalm\u00e1t \u00e9s k\u00e1ros\u00edthatja a sejteket. Sz\u00e9les k\u00f6rben haszn\u00e1lj\u00e1k a sejtek \u00f6reged\u00e9si modelljeinek r\u00f6vid id\u0151n bel\u00fcli el\u0151\u00e1ll\u00edt\u00e1s\u00e1ra. A sejtprolifer\u00e1ci\u00f3s aktivit\u00e1s fontos indik\u00e1tor annak meghat\u00e1roz\u00e1s\u00e1ra, hogy a teny\u00e9sztett sejtek k\u00e9pesek-e norm\u00e1lisan szaporodni bizonyos k\u00f6r\u00fclm\u00e9nyek k\u00f6z\u00f6tt. A CCK-8 k\u00eds\u00e9rlet eredm\u00e9nyei azt mutatt\u00e1k, hogy a PC12 sejtek t\u00fal\u00e9l\u00e9si ar\u00e1nya jelent\u0151sen cs\u00f6kkent a H2O2 koncentr\u00e1ci\u00f3 n\u00f6veked\u00e9s\u00e9vel, \u00e9s d\u00f3zisf\u00fcgg\u0151 hat\u00e1st mutatott. Amikor 300 \u03bc mol\/L H2O2-vel kezelt\u00e9k 4 \u00f3r\u00e1n kereszt\u00fcl, a sejtek t\u00fal\u00e9l\u00e9si ar\u00e1nya 60,64% volt. Ekkor a PC12 sejtek bizonyos m\u00e9rt\u00e9kben k\u00e1rosodtak, de m\u00e9g nem \u00e9rt\u00e9k el a visszaford\u00edthatatlan \u00e1llapotot. Ez\u00e9rt a 300 \u03bc mol\/L-t v\u00e1lasztottuk a PC12 sejtek oxidat\u00edv k\u00e1rosod\u00e1s\u00e1nak optim\u00e1lis koncentr\u00e1ci\u00f3jak\u00e9nt \u00e9s idejek\u00e9nt 4 \u00f3r\u00e1n kereszt\u00fcl a k\u00e9s\u0151bbi k\u00eds\u00e9rletek sor\u00e1n.<\/p>\n

Annak ellen\u0151rz\u00e9s\u00e9re, hogy a BRNP-nek van-e citotoxikus hat\u00e1sa a PC12 sejtekre, a BRNP-t \u00f6nmag\u00e1ban haszn\u00e1lt\u00e1k a PC12 sejtek H2O2-k\u00e1rosod\u00e1s n\u00e9lk\u00fcli kezel\u00e9s\u00e9re. A k\u00eds\u00e9rleti eredm\u00e9nyek azt mutatt\u00e1k, hogy a PC12 sejtek t\u00fal\u00e9l\u00e9si ar\u00e1nya nem v\u00e1ltozott jelent\u0151sen a BRNP k\u00fcl\u00f6nb\u00f6z\u0151 koncentr\u00e1ci\u00f3ival (7,8-2000 \u03bc g\/ml) t\u00f6rt\u00e9n\u0151 kezel\u00e9s ut\u00e1n (P>0,05), ami azt jelzi, hogy a BRNP-nek nincs jelent\u0151s citotoxicit\u00e1sa a PC12 sejtek \u00e9letk\u00e9pess\u00e9g\u00e9re. A BRNP hat\u00e1s\u00e1nak vizsg\u00e1latakor a H2O2 \u00e1ltal kiv\u00e1ltott k\u00e1rosod\u00e1sra a PC12 sejtekben az eredm\u00e9nyek azt mutatt\u00e1k, hogy a modellcsoporttal \u00f6sszehasonl\u00edtva a sejtek t\u00fal\u00e9l\u00e9si ar\u00e1nya fokozatosan n\u0151tt a BRNP koncentr\u00e1ci\u00f3j\u00e1nak n\u00f6veked\u00e9s\u00e9vel (P<0,05), \u00e9s a sejtek t\u00fal\u00e9l\u00e9si ar\u00e1nya jelent\u0151sen n\u0151tt, amikor a BRNP koncentr\u00e1ci\u00f3ja el\u00e9rte a 250 \u03bc g\/mL-t. A k\u00eds\u00e9rleti eredm\u00e9nyek alapj\u00e1n a BRNP bizonyos v\u00e9d\u0151hat\u00e1ssal rendelkezik a H2O2 \u00e1ltal kiv\u00e1ltott k\u00e1rosod\u00e1sra a PC12 sejtekben.
\nA lakt\u00e1t-dehidrogen\u00e1z (LDH) a sejtek belsej\u00e9ben l\u00e9tezik, \u00e9s k\u00e9pes kataliz\u00e1lni a lakt\u00e1t el\u0151\u00e1ll\u00edt\u00e1s\u00e1t a piruv\u00e1tb\u00f3l. A sejtek k\u00e1rosod\u00e1sakor az LDH nagy mennyis\u00e9gben szabadul fel, ami az extracellul\u00e1ris LDH-szint jelent\u0151s emelked\u00e9s\u00e9hez vezet. A felszabadul\u00f3 LDH mennyis\u00e9ge ar\u00e1nyos a k\u00e1rosod\u00e1s m\u00e9rt\u00e9k\u00e9vel, \u00edgy az LDH-sziv\u00e1rg\u00e1s m\u00e9rt\u00e9ke t\u00fckr\u00f6zheti a sejtk\u00e1rosod\u00e1s m\u00e9rt\u00e9k\u00e9t. A k\u00eds\u00e9rlet eredm\u00e9nyei arra utalnak, hogy a BRNP megakad\u00e1lyozhatja vagy lelass\u00edthatja a sejtmembr\u00e1n k\u00e1rosod\u00e1s\u00e1nak m\u00e9rt\u00e9k\u00e9t \u00e9s megakad\u00e1lyozhatja az intracellul\u00e1ris LDH sziv\u00e1rg\u00e1s\u00e1t.
\nA sejtben a mitokondriumok bels\u0151 membr\u00e1nj\u00e1nak mindk\u00e9t oldal\u00e1n nem egyenletes a protoneloszl\u00e1s, ami egy elektrok\u00e9miai gradienst, az \u00fagynevezett mitokondri\u00e1lis membr\u00e1npotenci\u00e1lt k\u00e9pez. Sz\u00e1mos tanulm\u00e1ny kimutatta, hogy amikor b\u00e1rmely apopt\u00f3zist induk\u00e1l\u00f3 faktor serkenti a sejt apopt\u00f3zis\u00e1t, a mitokondri\u00e1lis membr\u00e1npotenci\u00e1l cs\u00f6kken\u00e9se k\u00f6vetkezik be, \u00e9s ez a membr\u00e1npotenci\u00e1l-cs\u00f6kken\u00e9s hamarabb bek\u00f6vetkezik, mint a sejtmorfol\u00f3giai v\u00e1ltoz\u00e1sok. A mitokondri\u00e1lis membr\u00e1npotenci\u00e1l jelent\u0151s cs\u00f6kken\u00e9se a sejtek irreverzibilis apopt\u00f3zis\u00e1hoz vezet, ez\u00e9rt a mitokondri\u00e1lis membr\u00e1npotenci\u00e1l cs\u00f6kken\u00e9se a sejt apopt\u00f3zis korai szakasz\u00e1nak klasszikus jelens\u00e9g\u00e9nek tekinthet\u0151. Jelenleg a kutat\u00e1sok meger\u0151s\u00edtett\u00e9k a mitokondri\u00e1lis diszfunkci\u00f3 \u00e9s az \u00f6reged\u00e9si folyamat k\u00f6z\u00f6tti szoros kapcsolatot. P\u00e9ld\u00e1ul a mitokondri\u00e1lis energia (MMP \u00e9s ATP) el\u00e9gtelen ell\u00e1t\u00e1sa cs\u00f6kkentheti a szervezet anyagcsere-kapacit\u00e1s\u00e1t, ami egy sor \u00f6reged\u00e9si elv\u00e1ltoz\u00e1shoz vezet. Ezek k\u00f6z\u00fcl a mitokondri\u00e1lis membr\u00e1npotenci\u00e1l cs\u00f6kken\u00e9se a sejt apopt\u00f3zis egyik specifikus markere. A kutat\u00e1si eredm\u00e9nyek azt mutatt\u00e1k, hogy a PC12 sejtek mitokondri\u00e1lis membr\u00e1npotenci\u00e1lja jelent\u0151sen cs\u00f6kkent H2O2 kezel\u00e9s ut\u00e1n, \u00e9s a BRNP el\u0151kezel\u00e9ssel meg lehetett el\u0151zni a H2O2 \u00e1ltal okozott membr\u00e1npotenci\u00e1l cs\u00f6kken\u00e9st.
\nAz apopt\u00f3zis egy programozott sejthal\u00e1l folyamat, amely mag\u00e1ban foglalja az exog\u00e9n Fas\/FasL k\u00f6zvet\u00edtette hal\u00e1lreceptor \u00fatvonalakat, valamint az endog\u00e9n mitokondri\u00e1lis \u00e9s endoplazmatikus retikulum \u00fatvonalakat. Ezek k\u00f6z\u00fcl a mitokondri\u00e1lis k\u00f6zvet\u00edt\u00e9s\u0171 endog\u00e9n apopt\u00f3zis \u00fatvonal a programozott sejthal\u00e1l f\u0151 \u00fatvonala az eml\u0151ssejtekben. A pro-apoptotikus feh\u00e9rj\u00e9k \u00e1ltal k\u00e9pviselt Bax \u00e9s az antiapoptotikus feh\u00e9rj\u00e9k \u00e1ltal k\u00e9pviselt Bcl-2 a mitokondri\u00e1lis apopt\u00f3zisfaktorok felszabadul\u00e1s\u00e1t szab\u00e1lyoz\u00f3 f\u0151 feh\u00e9rj\u00e9k. A kaszp\u00e1z-prote\u00e1z az eml\u0151s\u00f6kben az apopt\u00f3zis egyik kulcsfontoss\u00e1g\u00fa effektorja, amelyek k\u00f6z\u00fcl a kaszp\u00e1z-3 a kaszp\u00e1z csal\u00e1d egyik legfontosabb prote\u00e1za, amely aktiv\u00e1lja a k\u00fcl\u00f6nb\u00f6z\u0151 apopt\u00f3zist serkent\u0151 faktorokat, \u00e9s a sejtek apopt\u00f3zis\u00e1nak fontos v\u00e9grehajt\u00f3jak\u00e9nt ismert. A BRNP bizonyos m\u00e9rt\u00e9kben cs\u00f6kkenti a kaszp\u00e1z-3 expresszi\u00f3j\u00e1t a H2O2 oxid\u00e1ci\u00f3val k\u00e1rosodott PC12 sejtekben. Felt\u00e9telezhet\u0151, hogy a BRNP cs\u00f6kkentheti a kaszp\u00e1z-3 expresszi\u00f3j\u00e1t, cs\u00f6kkentheti a sejt apopt\u00f3zist, \u00e9s \u00edgy k\u00e9sleltetheti a szervezet \u00f6reged\u00e9s\u00e9t. A BRNP-vel t\u00f6rt\u00e9n\u0151 beavatkoz\u00e1s ut\u00e1n a Bcl-2 expresszi\u00f3s szintje n\u0151tt az oxidat\u00edv k\u00e1rosod\u00e1ssal j\u00e1r\u00f3 PC12 sejtekben, m\u00edg a Bax \u00e9s a BRNP expresszi\u00f3s szintje k\u00fcl\u00f6nb\u00f6z\u0151 m\u00e9rt\u00e9kben cs\u00f6kkent. Ezek a kutat\u00e1si eredm\u00e9nyek arra utalhatnak, hogy a BRNP seg\u00edti a sejteket abban, hogy ellen\u00e1lljanak a mitokondriumokat \u00e9s a DNS-t \u00e9r\u0151 oxidat\u00edv stressz okozta k\u00e1rosod\u00e1snak, cs\u00f6kkenti a sejtek oxidat\u00edv k\u00e1rosod\u00e1s\u00e1t, \u00e9s fokozza a szervezet antioxid\u00e1ns stressz szab\u00e1lyoz\u00e1s\u00e1ra val\u00f3 k\u00e9pess\u00e9g\u00e9t.<\/p>","protected":false},"excerpt":{"rendered":"

Preliminary study on the protective mechanism of turnip neutral polysaccharides against oxidative damage in PC12 cells Turnip (Brassica rapa L.), also known as Chagu or Manjing, belongs to the Brassicaceae family and the Brassica genus. It is used as a medicinal herb with its roots and is rich in nutrients. It can be consumed for […]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"\nPreliminary study on the protective mechanism of turnip neutral polysaccharides against oxidative damage in PC12 cells - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/hu\/feherrepa-semleges-poliszacharidok\/\" \/>\n<meta property=\"og:locale\" content=\"hu_HU\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Preliminary study on the protective mechanism of turnip neutral polysaccharides against oxidative damage in PC12 cells - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"Preliminary study on the protective mechanism of turnip neutral polysaccharides against oxidative damage in PC12 cells Turnip (Brassica rapa L.), also known as Chagu or Manjing, belongs to the Brassicaceae family and the Brassica genus. It is used as a medicinal herb with its roots and is rich in nutrients. 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