{"id":6803,"date":"2024-08-06T14:44:15","date_gmt":"2024-08-06T14:44:15","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=6803"},"modified":"2024-08-06T14:44:15","modified_gmt":"2024-08-06T14:44:15","slug":"free-amino-acids-in-mulberry-leaves","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/hu\/free-amino-acids-in-mulberry-leaves\/","title":{"rendered":"F\u0151komponens-elemz\u00e9s \u00e9s a szabad aminosavak \u00e1tfog\u00f3 \u00e9rt\u00e9kel\u00e9se k\u00fcl\u00f6nb\u00f6z\u0151 eredet\u0171 \u00e9s fajt\u00e1j\u00fa eperfa levelekben"},"content":{"rendered":"

F\u0151komponens-elemz\u00e9s \u00e9s a szabad aminosavak \u00e1tfog\u00f3 \u00e9rt\u00e9kel\u00e9se k\u00fcl\u00f6nb\u00f6z\u0151 eredet\u0171 \u00e9s fajt\u00e1j\u00fa eperfa levelekben
\nAz eperfa leveleket el\u0151sz\u00f6r a \"Shennong Bencao Jing\"-ben jegyezt\u00e9k fel, \u00e9s az eperfa n\u00f6v\u00e9nyek sz\u00e1r\u00edtott leveleib\u0151l sz\u00e1rmazik. K\u00edna legt\u00f6bb r\u00e9sz\u00e9n elterjedt. Az eperfa levelei kiegyens\u00falyozott t\u00e1p\u00e9rt\u00e9kkel rendelkeznek, \u00e9s \"halhatatlan levelek\" n\u00e9ven ismertek. Jellegzetes gy\u00f3gy\u00e1szati \u00e9s ehet\u0151 forr\u00e1s is, amely gazdag t\u00e1panyagokat tartalmaz k\u00fcl\u00f6nleges \u00e9lettani aktivit\u00e1ssal, mint p\u00e9ld\u00e1ul feh\u00e9rj\u00e9t, szabad aminosavakat, alkaloidokat, flavonoidokat stb. Term\u00e9szetes nyersanyagk\u00e9nt szolg\u00e1lnak gy\u00f3gyszerek vagy funkcion\u00e1lis \u00e9lelmiszerek kifejleszt\u00e9s\u00e9hez. A jelent\u00e9sek szerint a szabad aminosavak (FAA-k) az eperfa levelekben a teljes sz\u00e1razanyag 0,2%-2,0%-j\u00e9t tehetik ki, \u00e9s a FAA-k \u00f6sszet\u00e9tele \u00e9s tartalma jelent\u0151s hat\u00e1ssal van az eperfa levelek \u00edzmin\u0151s\u00e9g\u00e9re, k\u00f6zvetlen\u00fcl vagy k\u00f6zvetve befoly\u00e1solva az eperfa lev\u00e9lterm\u00e9kek min\u0151s\u00e9g\u00e9t. Ez\u00e9rt az eperfa lev\u00e9l FFA-anyagainak \u00e1tfog\u00f3 elemz\u00e9se \u00e9s \u00e9rt\u00e9kel\u00e9se nagy jelent\u0151s\u00e9ggel b\u00edr. Jelenleg k\u00fcl\u00f6nb\u00f6z\u0151 m\u00f3dszerek \u00e1llnak rendelkez\u00e9sre az FFA-k meghat\u00e1roz\u00e1s\u00e1ra, \u00e9s alkalmaz\u00e1suk kutat\u00e1si el\u0151rehalad\u00e1sa gyors. Vannak kutat\u00e1si jelent\u00e9sek a spektrofotometria alkalmaz\u00e1s\u00e1r\u00f3l a tea, gy\u00f3gy\u00e1szati anyagok stb. aminosavtartalm\u00e1nak meghat\u00e1roz\u00e1s\u00e1ra. Xia \u00e9s munkat\u00e1rsai spektrofotometri\u00e1t haszn\u00e1ltak 23 eperfa fajta eperfa level\u00e9nek GABA-tartalm\u00e1nak meghat\u00e1roz\u00e1s\u00e1ra. A Huosang eperfa fajta friss eperfa leveleinek GABA-tartalma a legmagasabb, el\u00e9rte az 1,224 mg\/g-ot, ami 3,76-szorosa a legalacsonyabb tartalm\u00fa eperfa fajt\u00e1\u00e9hoz k\u00e9pest. Deng \u00e9s munkat\u00e1rsai az eperfa levelek teljes aminosavtartalm\u00e1t aminosav-analiz\u00e1torral 8,62-10,63g\/100g, m\u00edg Yang \u00e9s munkat\u00e1rsai az eperfa levelek esszenci\u00e1lis aminosavtartalm\u00e1t 29,11g\/100g nyersfeh\u00e9rj\u00e9ben hat\u00e1rozt\u00e1k meg. Zhen \u00e9s munkat\u00e1rsai HPLC-t haszn\u00e1ltak a szabad aminosavak tartalm\u00e1nak kimutat\u00e1s\u00e1ra az eperfa levelekben, amelyeket h\u00e1rom k\u00fcl\u00f6nb\u00f6z\u0151 \u00e9vszakban sz\u00fcretelt eperfa fajt\u00e1kb\u00f3l szedtek le. Azt tal\u00e1lt\u00e1k, hogy a szerin-tartalom, amely a tavaszi eperfa levelekben magasabb volt, cs\u00f6kkent az els\u0151 fagyszezonban, m\u00edg a treonin-tartalom, amely a tavaszi eperfa levelekben alacsonyabb volt, n\u0151tt az els\u0151 fagyszezonban. Lu \u00e9s munkat\u00e1rsai l\u00e9trehoztak egy m\u00f3dszert az aminosavak, nukleozidok \u00e9s alkaloidok egyidej\u0171 meghat\u00e1roz\u00e1s\u00e1ra eperfa lev\u00e9lmint\u00e1kban ultranagy teljes\u00edtm\u00e9ny\u0171 folyad\u00e9kkromatogr\u00e1fi\u00e1s tandem tripla kvadrupol t\u00f6megspektrometria seg\u00edts\u00e9g\u00e9vel. A spektrofotometri\u00e1s m\u00f3dszer egyszer\u0171 \u00e9s k\u00f6nnyen kivitelezhet\u0151, alkalmas az arom\u00e1s szabad aminosavak kimutat\u00e1s\u00e1ra, de a line\u00e1ris tartom\u00e1ny kicsi, csak egy nagys\u00e1grend; Az aminosav-analiz\u00e1toros m\u00f3dszer pontos, megb\u00edzhat\u00f3 \u00e9s j\u00f3 reproduk\u00e1lhat\u00f3s\u00e1ggal rendelkezik, \u00e9s k\u00e9pes a legt\u00f6bb t\u00edpus\u00fa aminosav \u00e9s homol\u00f3gjaik meghat\u00e1roz\u00e1s\u00e1ra. Ennek a m\u00f3dszernek azonban viszonylag hossz\u00fa a meghat\u00e1roz\u00e1si ideje, nagy az oszlop ut\u00e1ni diff\u00fazi\u00f3ja, ami a felbont\u00e1s cs\u00f6kken\u00e9s\u00e9t eredm\u00e9nyezi, \u00e9s a m\u0171szer fel\u00e9p\u00edt\u00e9se bonyolult \u00e9s viszonylag dr\u00e1ga; A nagy teljes\u00edtm\u00e9ny\u0171 folyad\u00e9kkromatogr\u00e1fia \u00e9s a nagy teljes\u00edtm\u00e9ny\u0171 folyad\u00e9kkromatogr\u00e1fia-t\u00f6megspektrometria el\u0151nye a nagy szelektivit\u00e1s, a nagy specificit\u00e1s, a nagy \u00e9rz\u00e9kenys\u00e9g \u00e9s a mint\u00e1k sz\u00e9les k\u00f6r\u0171 alkalmazhat\u00f3s\u00e1ga. Haszn\u00e1latukat azonban korl\u00e1tozza a magas m\u0171szer\u00e1r, a nagyteljes\u00edtm\u00e9ny\u0171 folyad\u00e9kkromatogr\u00e1fi\u00e1b\u00f3l sz\u00e1rmaz\u00f3 mell\u00e9kterm\u00e9kek \u00e9s a sz\u00e1rmaz\u00e9kok hidrol\u00edzise \u00e1ltal okozott interferencia. A nagy teljes\u00edtm\u00e9ny\u0171 folyad\u00e9kkromatogr\u00e1fia \u00e9s fluoreszcens detekt\u00e1l\u00e1s kombin\u00e1ci\u00f3ja (HPLC-FLD) m\u00f3dszer az aminosavtartalom meghat\u00e1roz\u00e1s\u00e1ra a nagy specificit\u00e1s, a nagy \u00e9rz\u00e9kenys\u00e9g, az egyszer\u0171s\u00edtett \u00e9s neh\u00e9zkes el\u0151kezel\u00e9si l\u00e9p\u00e9sek, a ler\u00f6vid\u00edtett el\u0151kezel\u00e9si id\u0151, valamint az er\u0151s b\u00e1zisok haszn\u00e1lat\u00e1nak \u00e9s a magas h\u0151m\u00e9rs\u00e9klettel val\u00f3 \u00e9rintkez\u00e9snek a sz\u00fcks\u00e9gtelens\u00e9ge, ami biztons\u00e1gosabb. Fang \u00e9s munkat\u00e1rsai HPLC-FLD m\u00f3dszert alkalmaztak 18 szabad aminosav c\u00e9lzott kimutat\u00e1s\u00e1ra friss \u00e9s sz\u00e1r\u00edtott tealevelekben, ami referenci\u00e1t szolg\u00e1ltatott ehhez a tanulm\u00e1nyhoz. Kor\u00e1bbi tanulm\u00e1nyok kimutatt\u00e1k, hogy az olyan t\u00e9nyez\u0151k, mint a k\u00fcl\u00f6nb\u00f6z\u0151 sz\u00e1rmaz\u00e1s, a fajt\u00e1k, a sz\u00e1r\u00edt\u00e1si m\u00f3dszerek \u00e9s a betakar\u00edt\u00e1si id\u0151szakok jelent\u0151s hat\u00e1ssal vannak az eperfa levelek aminosavtartalm\u00e1ra. Ez\u00e9rt ebben a tanulm\u00e1nyban 17 k\u00fcl\u00f6nb\u00f6z\u0151 eredet\u0171 eperfa levelet gy\u0171jt\u00f6tt\u00fcnk Chongqingban, bele\u00e9rtve h\u00e1rom fajt\u00e1t, novemberben (korai fagy), \u00e9s 45 \u2103-on sz\u00e1r\u00edtottuk \u0151ket \u00e1lland\u00f3 t\u00f6meg\u0171re. HPLC-FLD-t haszn\u00e1ltunk a 18 szabad aminosav felhalmoz\u00f3d\u00e1si k\u00fcl\u00f6nbs\u00e9geinek meghat\u00e1roz\u00e1s\u00e1ra a k\u00fcl\u00f6nb\u00f6z\u0151 eperfa lev\u00e9lmint\u00e1kban, \u00e9s TAV elemz\u00e9st \u00e9s SPSS szoftverrel kapcsolatos statisztikai elemz\u00e9si technik\u00e1kat haszn\u00e1ltunk a k\u00fcl\u00f6nb\u00f6z\u0151 eredet\u0171 Morus alba L. \u00e9s Huasang M. Cathayana Hemsl. \u00e9s Chicken Mulberry M A szabad aminosavak t\u00e1pl\u00e1lkoz\u00e1si \u00e9s \u00edzjellemz\u0151i h\u00e1rom eperfa lev\u00e9lben, Austalis Poi. Ez t\u00e1mpontot ny\u00fajt az eperfaforr\u00e1sok hasznos\u00edt\u00e1s\u00e1hoz \u00e9s min\u0151s\u00e9g\u00fck kialak\u00edt\u00e1s\u00e1hoz.<\/p>\n

\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"<\/p>\n

Az eperfa levelei gazdag t\u00e1panyagokat \u00e9s bioakt\u00edv anyagokat, valamint k\u00fcl\u00f6nb\u00f6z\u0151 aminosavakat tartalmaznak. Funkcion\u00e1lis \u00e9s biol\u00f3giai aktivit\u00e1sukat sz\u00e9les k\u00f6rben tanulm\u00e1nyozt\u00e1k. Zhang kutat\u00e1sai azt mutatj\u00e1k, hogy az eperfa levelek aminosavtartalma hasonl\u00f3 a repcepollen\u00e9hez, amely 8 esszenci\u00e1lis aminosavat tartalmaz, amelyekre az emberi szervezetnek sz\u00fcks\u00e9ge van. Az \u00fcltet\u00e9si er\u0151forr\u00e1sok b\u0151s\u00e9gesek, \u00edgy j\u00f3 er\u0151forr\u00e1s a funkcion\u00e1lis \u00e9lelmiszern\u00f6v\u00e9nyek fejleszt\u00e9s\u00e9hez; Deng \u00e9s t\u00e1rsai \u00e9rt\u00e9kelt\u00e9k a k\u00fcl\u00f6nb\u00f6z\u0151 r\u00e9gi\u00f3kb\u00f3l sz\u00e1rmaz\u00f3 eperfa levelek min\u0151s\u00e9g\u00e9t, \u00e9s meg\u00e1llap\u00edtott\u00e1k, hogy a k\u00fcl\u00f6nb\u00f6z\u0151 r\u00e9gi\u00f3kb\u00f3l sz\u00e1rmaz\u00f3 eperfa levelek k\u00e9miai t\u00edpusai k\u00f6z\u00f6tt nem voltak jelent\u0151s k\u00fcl\u00f6nbs\u00e9gek, de az \u00f6sszetev\u0151k tartalm\u00e1ban voltak k\u00fcl\u00f6nbs\u00e9gek, ami t\u00fckr\u00f6zi a k\u00fcl\u00f6nb\u00f6z\u0151 r\u00e9gi\u00f3kb\u00f3l sz\u00e1rmaz\u00f3 eperfa levelek k\u00e9miai \u00f6sszet\u00e9tel\u00e9nek jellemz\u0151it.
\nEbben a k\u00eds\u00e9rletben h\u00e1romf\u00e9le eredet\u0171 eperfa, k\u00ednai eperfa \u00e9s csirke eperfa lev\u00e9lmint\u00e1k FFA-\u00e9rt\u00e9keit vizsg\u00e1lt\u00e1k. A legmagasabb teljes aminosavtartalom el\u00e9rte az 1927,33 mg\/100 g-ot, magas esszenci\u00e1lis, f\u00e9lig esszenci\u00e1lis \u00e9s nem esszenci\u00e1lis aminosavakkal. Ez az \u00e9rt\u00e9k az aminosavakat tartalmaz\u00f3 funkcion\u00e1lis t\u00e1pl\u00e1lkoz\u00e1si \u00e9lelmiszerek fejleszt\u00e9s\u00e9nek \u00e9rt\u00e9ke. A TAV-\u00e9rt\u00e9k az \u00edzl\u00e9s biztos\u00edt\u00e1s\u00e1nak referencia mutat\u00f3jak\u00e9nt szolg\u00e1l. Az aminosav TAV-elemz\u00e9ssel meg\u00e1llap\u00edtott\u00e1k, hogy a h\u00e1rom fajta k\u00fcl\u00f6nb\u00f6z\u0151 r\u00e9gi\u00f3ib\u00f3l sz\u00e1rmaz\u00f3 eperfa levelekben kevesebb olyan aminosav van, amelynek TAV-\u00e9rt\u00e9ke nagyobb, mint 1. A h\u00e1rom fajt\u00e1b\u00f3l sz\u00e1rmaz\u00f3 eperfa levelekben kevesebb olyan aminosav van, amelynek TAV-\u00e9rt\u00e9ke 1-n\u00e9l nagyobb. B\u00e1r az \u00edzhez val\u00f3 hozz\u00e1j\u00e1rul\u00e1suk viszonylag kicsi, nem j\u00e1rulnak hozz\u00e1 az \u00edzhez. Az \u00edz \u00f6sszet\u00e9tele nem kiz\u00e1r\u00f3lag az 1-n\u00e9l nagyobb TAV-\u00e9rt\u00e9kkel rendelkez\u0151 \u00edzkomponensek t\u00e9nyez\u0151inek k\u00f6sz\u00f6nhet\u0151. Az eperfa levelekben tal\u00e1lhat\u00f3 sz\u00e1mos \u00edzkomponensnek t\u00f6bb \u00edzjellemz\u0151je van, \u00e9s tov\u00e1bbi kutat\u00e1sokra van sz\u00fcks\u00e9g az egyes aminosavkomponensek tov\u00e1bbi vizsg\u00e1lat\u00e1ra, hogy felt\u00e1rjuk a k\u00f6zt\u00fck l\u00e9v\u0151 k\u00f6lcs\u00f6nhat\u00e1sokat. A f\u0151komponens-elemz\u00e9ssel meg\u00e1llap\u00edtott\u00e1k, hogy a k\u00fcl\u00f6nb\u00f6z\u0151 r\u00e9gi\u00f3kb\u00f3l sz\u00e1rmaz\u00f3 h\u00e1romf\u00e9le eperfa lev\u00e9l k\u00f6z\u00f6tt jelent\u0151s k\u00fcl\u00f6nbs\u00e9gek vannak az aminosavak tekintet\u00e9ben, amelyek k\u00f6z\u00fcl a Chongqing-i Banan ker\u00fclet, Anlan v\u00e1ros\u00e1ban tal\u00e1lhat\u00f3 eperfa levelek aminosavtartalma \u00e9s min\u0151s\u00e9ge volt a legmagasabb. Ez\u00e9rt az eperfa levelek \u00fcltet\u00e9si alapjainak l\u00e9trehoz\u00e1sakor a csirkeeper \u00e9s a k\u00ednai eperfa fajt\u00e1k sz\u00e9les k\u00f6rben \u00fcltethet\u0151k a r\u00e9gi\u00f3 f\u00f6ldrajzi \u00e9s k\u00f6rnyezeti el\u0151nyei alapj\u00e1n. Az eperfa levelek ezen a ter\u00fcleten a tov\u00e1bbi fejleszt\u00e9s \u00e9s felhaszn\u00e1l\u00e1s szempontj\u00e1b\u00f3l el\u0151nyben r\u00e9szes\u00edtett fajt\u00e1k. Ez a tanulm\u00e1ny a k\u00fcl\u00f6nb\u00f6z\u0151 fajt\u00e1kb\u00f3l \u00e9s eredet\u0171 eperfa levelek aminosav-\u00f6sszet\u00e9tel\u00e9t vizsg\u00e1lta, referenci\u00e1t ny\u00fajtva az eperfa levelek term\u00e9kmin\u0151s\u00e9g\u00e9nek \u00e9rt\u00e9kel\u00e9s\u00e9hez \u00e9s a funkcion\u00e1lis term\u00e9kek fejleszt\u00e9s\u00e9hez \u00e9s felhaszn\u00e1l\u00e1s\u00e1hoz.<\/p>","protected":false},"excerpt":{"rendered":"

Principal component analysis and comprehensive evaluation of free amino acids in mulberry leaves from different origins and varieties Mulberry leaves were first recorded in the “Shennong Bencao Jing” and originated from the dried leaves of mulberry plants. They are distributed in most parts of China. Mulberry leaves have balanced nutritional value and are known as […]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"\nPrincipal component analysis and comprehensive evaluation of free amino acids in mulberry leaves from different origins and varieties - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/hu\/free-amino-acids-in-mulberry-leaves\/\" \/>\n<meta property=\"og:locale\" content=\"hu_HU\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Principal component analysis and comprehensive evaluation of free amino acids in mulberry leaves from different origins and varieties - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"Principal component analysis and comprehensive evaluation of free amino acids in mulberry leaves from different origins and varieties Mulberry leaves were first recorded in the “Shennong Bencao Jing” and originated from the dried leaves of mulberry plants. They are distributed in most parts of China. 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