{"id":6627,"date":"2024-08-06T12:47:33","date_gmt":"2024-08-06T12:47:33","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=6627"},"modified":"2024-08-06T12:47:33","modified_gmt":"2024-08-06T12:47:33","slug":"xihuangcao-medicinal-herbs","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/hu\/xihuangcao-medicinal-herbs\/","title":{"rendered":"A Xihuangcao gy\u00f3gyn\u00f6v\u00e9nyek n\u00e9gy eredeti n\u00f6v\u00e9ny\u00e9nek UPLC jellegzetes spektrum\u00e1nak azonos\u00edt\u00e1sa"},"content":{"rendered":"<p>A Xihuangcao gy\u00f3gyn\u00f6v\u00e9nyek n\u00e9gy eredeti n\u00f6v\u00e9ny\u00e9nek UPLC jellegzetes spektrum\u00e1nak azonos\u00edt\u00e1sa<br \/>\nA Xihuang Grass az \u00d6t Hegyh\u00e1t d\u00e9li r\u00e9sz\u00e9n tal\u00e1lhat\u00f3 egyed\u00fcl\u00e1ll\u00f3 k\u00ednai gy\u00f3gyn\u00f6v\u00e9ny, amelynek hat\u00e1sa a h\u0151tiszt\u00edt\u00e1s \u00e9s a nedvess\u00e9g elt\u00e1vol\u00edt\u00e1sa, a v\u00e9r h\u0171t\u00e9se \u00e9s a pang\u00e1s megsz\u00fcntet\u00e9se. Nedves h\u0151s\u00e9gs\u00e1rgas\u00e1g, hasi puffad\u00e1s \u00e9s hipochondri\u00e1s f\u00e1jdalom, nedves h\u0151s\u00e9g hasmen\u00e9s, h\u0151toxin hasmen\u00e9s \u00e9s traum\u00e1s s\u00e9r\u00fcl\u00e9sek eset\u00e9n alkalmazz\u00e1k. Az \u00d6t Hegyh\u00e1t d\u00e9li r\u00e9sz\u00e9n gyakran haszn\u00e1lt klinikai gy\u00f3gyszer, \u00e9s a h\u00edres hagyom\u00e1nyos k\u00ednai szabadalmi gy\u00f3gyszerek \u00e9s egyszer\u0171 k\u00e9sz\u00edtm\u00e9nyek nagy v\u00e1laszt\u00e9k\u00fa \"gyullad\u00e1scs\u00f6kkent\u0151 \u00e9s epehajt\u00f3 tablett\u00e1k\" receptjeinek nyersanyaga is. A K\u00ednai Gy\u00f3gyszerk\u00f6nyv (2020-as kiad\u00e1s, 4. k\u00f6tet) a Rabdosia lophanthoides (Buch. - Ham. ex D. DON) H. Hara vagy Rabdosia serora (Maxim.) H. Hrar, a Labiatae csal\u00e1dba \u00e9s a Rabdosia nemzets\u00e9gbe tartoz\u00f3 n\u00f6v\u00e9ny sz\u00e1r\u00edtott f\u00f6ld feletti r\u00e9szek\u00e9nt tartja nyilv\u00e1n. A Guangdong tartom\u00e1nyi k\u00ednai gy\u00f3gyn\u00f6v\u00e9ny-gy\u00f3gy\u00e1szati szabv\u00e1nyokban szerepl\u0151 eredeti n\u00f6v\u00e9ny a Rabdosia lophanthoides R serra\u3001 Fonalas mint\u00e1s illatos tea \u00e9s z\u00f6lds\u00e9g R Lophanthoides \u00e9s rostos vir\u00e1g\u00fa v\u00e1ltozata R lophanthoides var. graciliflora(Benth.) H. Hrar\u3002 A Xihuangcao gy\u00f3gy\u00e1szati anyagok cs\u00edraplazmaforr\u00e1sainak kutat\u00e1sa \u00e9s rendszerez\u00e9se sor\u00e1n kutat\u00f3csoportunk tagjai meg\u00e1llap\u00edtott\u00e1k, hogy az ugyanahhoz a nemzets\u00e9ghez tartoz\u00f3 Rabdosia stracheyi (Benth. ex Hook. f.) Hara n\u00f6v\u00e9nyt a n\u00e9pi kult\u00far\u00e1ban is gyakran haszn\u00e1lj\u00e1k Xihuangcao gy\u00f3gy\u00e1szati anyagk\u00e9nt. E n\u00f6v\u00e9nyek hasonl\u00f3 morfol\u00f3giai jellemz\u0151i miatt neh\u00e9z megk\u00fcl\u00f6nb\u00f6ztetni a sz\u00e1raz gy\u00f3gy\u00e1szati anyagokat megjelen\u00e9s\u00fck alapj\u00e1n, \u00e9s mindig is fenn\u00e1llt a z\u0171rzavar jelens\u00e9ge a Xihuangcao kereskedelmi gy\u00f3gy\u00e1szati anyagok fajtak\u00e9szleteiben. Ez\u00e9rt nagy jelent\u0151s\u00e9ggel b\u00edr a k\u00fcl\u00f6nb\u00f6z\u0151 Huangcao-t\u00edpusok azonos\u00edt\u00e1si m\u00f3dszereinek kidolgoz\u00e1sa a klinikai gy\u00f3gyszerbiztons\u00e1g \u00e9s hat\u00e9konys\u00e1g biztos\u00edt\u00e1sa \u00e9s jav\u00edt\u00e1sa \u00e9rdek\u00e9ben.<\/p>\n<p>A Xihuangcao gy\u00f3gy\u00e1szati anyagok k\u00fcl\u00f6nb\u00f6z\u0151 eredeti n\u00f6v\u00e9nyeinek azonos\u00edt\u00e1s\u00e1t hagyom\u00e1nyosan a morfol\u00f3gia \u00e9s a mikroszk\u00f3pia szempontjai alapj\u00e1n tanulm\u00e1nyozt\u00e1k. Mivel azonban a sz\u00e1raz gy\u00f3gy\u00e1szati anyagok gyakran gy\u0171r\u00f6ttek \u00e9s t\u00f6r\u00f6ttek, neh\u00e9z a pontos \u00e9s gyors azonos\u00edt\u00e1suk. Az elm\u00falt \u00e9vekben a tud\u00f3sok a DNS-molekul\u00e1k \u00e9s a k\u00e9miai \u00f6sszetev\u0151k szintj\u00e9n v\u00e9geztek azonos\u00edt\u00e1si vizsg\u00e1latokat az eredeti n\u00f6v\u00e9nyeken. A molekul\u00e1ris azonos\u00edt\u00e1s azonban csak fajszinten k\u00e9pes k\u00fcl\u00f6nbs\u00e9get tenni a Rabdosia serora \u00e9s a Lycaena angustifolia k\u00f6z\u00f6tt, \u00edgy neh\u00e9z megk\u00fcl\u00f6nb\u00f6ztetni a Lycaena angustifolia eredeti \u00e9s v\u00e1ltozatos fajt\u00e1it. Ami a k\u00e9miai \u00f6sszet\u00e9tel kutat\u00e1s\u00e1t illeti, egyes tud\u00f3sok \u00fagy v\u00e9lik, hogy a Fourier-transzform\u00e1ci\u00f3s infrav\u00f6r\u00f6s spektroszk\u00f3pia \u00e9s a k\u00e9tdimenzi\u00f3s korrel\u00e1ci\u00f3s infrav\u00f6r\u00f6s spektroszk\u00f3pia k\u00e9pes el\u00e9rni a Xihuangcao, a b\u00e9lelt illatos te\u00e1s z\u00f6lds\u00e9g \u00e9s a sz\u00e1las vir\u00e1g\u00fa \u00e9s keskeny alapon term\u0151 fajt\u00e1k azonos\u00edt\u00e1s\u00e1t. N\u00e9h\u00e1ny tud\u00f3s a k\u00e9miai \u00f6sszet\u00e9tel-tartalom \u00e9s az ujjlenyomat-spektrumok azonos\u00edt\u00e1s\u00e1ra is v\u00e9gzett kutat\u00e1sokat, de a k\u00eds\u00e9rleti eredm\u00e9nyek nem tudj\u00e1k j\u00f3l megk\u00fcl\u00f6nb\u00f6ztetni a b\u00e9lelt illatos te\u00e1s z\u00f6lds\u00e9g eredeti fajt\u00e1j\u00e1t a v\u00e1ltozatait\u00f3l. Az ut\u00f3bbi \u00e9vekben a kemometriai m\u00f3dszereket egyre sz\u00e9lesebb k\u00f6rben haszn\u00e1lj\u00e1k a hagyom\u00e1nyos k\u00ednai gy\u00f3gy\u00e1szat kromatogr\u00e1fi\u00e1s elemz\u00e9s\u00e9ben. A kromatogr\u00e1fi\u00e1s elemz\u00e9s \u00e1ltal gener\u00e1lt nagy mennyis\u00e9g\u0171 adat tov\u00e1bbi elemz\u00e9s\u00e9re \u00e9s feldolgoz\u00e1s\u00e1ra alkalmasak, \u00e9s a kromatogr\u00e1fi\u00e1s elemz\u00e9ssel kombin\u00e1lva egyre fontosabb szerepet j\u00e1tszanak a hagyom\u00e1nyos k\u00ednai gy\u00f3gy\u00e1szat min\u0151s\u00e9gellen\u0151rz\u00e9s\u00e9ben. Az ultra nagy teljes\u00edtm\u00e9ny\u0171 folyad\u00e9kkromatogr\u00e1fia (UPLC) el\u0151nye a r\u00f6vid elemz\u00e9si id\u0151, a j\u00f3 elv\u00e1laszt\u00e1si hat\u00e1s \u00e9s a nagy \u00e9rz\u00e9kenys\u00e9g. Ez\u00e9rt ez a tanulm\u00e1ny az UPLC-t haszn\u00e1lta a n\u00e9gy k\u00fcl\u00f6nb\u00f6z\u0151 alapn\u00f6v\u00e9nyb\u0151l, nevezetesen a Xihuangcao, a Xianwen Xiangchacai, a Xianxiang Xiangchacai \u00e9s a Longye Xiangchacai 32 t\u00e9tel \u00f6sszegy\u0171jt\u00f6tt gy\u00f3gy\u00e1szati anyag\u00e1nak jellemz\u0151 spektrum\u00e1nak vizsg\u00e1lat\u00e1ra, \u00e9s kemometriai m\u00f3dszerekkel, p\u00e9ld\u00e1ul klaszterelemz\u00e9ssel, f\u0151komponens-elemz\u00e9ssel \u00e9s r\u00e9szleges legkisebb n\u00e9gyzetek diszkriminancia-elemz\u00e9ssel kombin\u00e1lva, hogy megk\u00fcl\u00f6nb\u00f6ztesse \u00e9s azonos\u00edtsa ezt a n\u00e9gy alapn\u00f6v\u00e9nyt, annak \u00e9rdek\u00e9ben, hogy \u00e9rt\u00e9kes betekint\u00e9st ny\u00fajtson a Xixi sz\u00e1m\u00e1ra. Referenci\u00e1t ny\u00fajt a Huangcao gy\u00f3gy\u00e1szati anyagok azonos\u00edt\u00e1s\u00e1hoz, valamint min\u0151s\u00e9g\u00fck elemz\u00e9s\u00e9hez \u00e9s \u00e9rt\u00e9kel\u00e9s\u00e9hez.<\/p>\n<p>&nbsp;<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-6628\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-244.png\" alt=\"\" width=\"365\" height=\"672\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-6629\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/2-204.png\" alt=\"\" width=\"787\" height=\"231\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/2-204.png 787w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/2-204-768x225.png 768w\" sizes=\"(max-width: 787px) 100vw, 787px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-6630\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/3-191.png\" alt=\"\" width=\"783\" height=\"368\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/3-191.png 783w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/3-191-768x361.png 768w\" sizes=\"(max-width: 783px) 100vw, 783px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-6631\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/4-171.png\" alt=\"\" width=\"784\" height=\"781\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/4-171.png 784w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/4-171-768x765.png 768w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/4-171-723x720.png 723w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/4-171-200x200.png 200w\" sizes=\"(max-width: 784px) 100vw, 784px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-6632\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/5-154.png\" alt=\"\" width=\"361\" height=\"787\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/5-154.png 361w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/5-154-330x720.png 330w\" sizes=\"(max-width: 361px) 100vw, 361px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-6633\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/6-140.png\" alt=\"\" width=\"787\" height=\"186\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/6-140.png 787w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/6-140-768x182.png 768w\" sizes=\"(max-width: 787px) 100vw, 787px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-6634\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/7-128.png\" alt=\"\" width=\"775\" height=\"538\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/7-128.png 775w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/7-128-768x533.png 768w\" sizes=\"(max-width: 775px) 100vw, 775px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-6635\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/8-116.png\" alt=\"\" width=\"781\" height=\"486\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/8-116.png 781w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/8-116-768x478.png 768w\" sizes=\"(max-width: 781px) 100vw, 781px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-6636\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/9-108.png\" alt=\"\" width=\"784\" height=\"381\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/9-108.png 784w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/9-108-768x373.png 768w\" sizes=\"(max-width: 784px) 100vw, 784px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-6637\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/10-86.png\" alt=\"\" width=\"783\" height=\"370\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/10-86.png 783w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/10-86-768x363.png 768w\" sizes=\"(max-width: 783px) 100vw, 783px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-6638\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/11-78.png\" alt=\"\" width=\"776\" height=\"235\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/11-78.png 776w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/11-78-768x233.png 768w\" sizes=\"(max-width: 776px) 100vw, 776px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-6639\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/12-61.png\" alt=\"\" width=\"781\" height=\"258\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/12-61.png 781w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/12-61-768x254.png 768w\" sizes=\"(max-width: 781px) 100vw, 781px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-6640\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/13-54.png\" alt=\"\" width=\"780\" height=\"288\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/13-54.png 780w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/13-54-768x284.png 768w\" sizes=\"(max-width: 780px) 100vw, 780px\" \/><\/p>\n<p>Ebben a tanulm\u00e1nyban a vizsg\u00e1lati oldat el\u0151\u00e1ll\u00edt\u00e1si m\u00f3dszereit \u00e9s kromatogr\u00e1fi\u00e1s k\u00f6r\u00fclm\u00e9nyeit vizsg\u00e1lt\u00e1k \u00e9s optimaliz\u00e1lt\u00e1k. Az extrakci\u00f3s old\u00f3szerek (metanol, 80% metanol, 60% metanol) vizsg\u00e1lati eredm\u00e9nyei azt mutatt\u00e1k, hogy amikor metanolt haszn\u00e1ltak extrakci\u00f3s old\u00f3szerk\u00e9nt, a kapott minta kromatogramja t\u00f6bb kromatogr\u00e1fi\u00e1s cs\u00facsot, viszonylag nagyobb cs\u00facster\u00fcleteket \u00e9s stabil alapvonalat mutatott, ami jobban t\u00fckr\u00f6zte a Xihuangcao gy\u00f3gy\u00e1szati anyag k\u00e9miai \u00f6sszet\u00e9tel\u00e9t. Ez\u00e9rt a Xihuangcao gy\u00f3gy\u00e1szati anyag jellegzetes spektrum\u00e1hoz a metanolt v\u00e1lasztott\u00e1k extrakci\u00f3s old\u00f3szerk\u00e9nt; A hull\u00e1mhosszok (250, 254, 260, 280 nm) m\u00e9r\u00e9si eredm\u00e9nyei azt mutatt\u00e1k, hogy 254 nm-en sok kromatogr\u00e1fi\u00e1s cs\u00facs volt, \u00e9s az egyes cs\u00facsok m\u00e9rete egyenletes volt. Ez\u00e9rt a 254nm-t v\u00e1lasztottuk detekt\u00e1l\u00e1si hull\u00e1mhossznak; A mobil f\u00e1zisok (metanol -0,1% hangyasav v\u00edz, acetonitril -0,1% hangyasav v\u00edz, 0,1% hangyasav metanol -0,1% hangyasav v\u00edz \u00e9s 0,1% hangyasav acetonitril -0,1% hangyasav v\u00edz) vizsg\u00e1lati eredm\u00e9nyei azt mutatt\u00e1k, hogy metanol -0.1% hangyasav-vizet haszn\u00e1ltunk mobilf\u00e1zisk\u00e9nt az el\u00faci\u00f3hoz, az alapvonal lapos volt, \u00e9s az elv\u00e1laszt\u00e1si fok \u00e9s a cs\u00facs alakja j\u00f3 volt; Az oszloph\u0151m\u00e9rs\u00e9klet \u00e9s az \u00e1raml\u00e1si sebess\u00e9g vizsg\u00e1lat\u00e1nak eredm\u00e9nyei azt mutatt\u00e1k, hogy az egyes kromatogr\u00e1fi\u00e1s m\u00f3dszerek elv\u00e1laszt\u00e1si hat\u00e9konys\u00e1ga akkor volt a legjobb, amikor az oszlop h\u0151m\u00e9rs\u00e9klete 30 \u2103 \u00e9s az \u00e1raml\u00e1si sebess\u00e9g 0,3mL\/min volt.<\/p>\n<p>Ebben a tanulm\u00e1nyban az UPLC-t haszn\u00e1lt\u00e1k a Xihuangcao gy\u00f3gyn\u00f6v\u00e9ny n\u00e9gy alapn\u00f6v\u00e9ny\u00e9nek azonos\u00edt\u00e1s\u00e1ra alkalmazhat\u00f3 jellegzetes t\u00e9rk\u00e9p l\u00e9trehoz\u00e1s\u00e1ra. Az alapvonal lapos volt, az elv\u00e1laszt\u00e1si fok j\u00f3 volt, \u00e9s a k\u00f6z\u00f6s cs\u00facsok sz\u00e1ma nagy volt. A Xihuangcao, a Xianwen Xiangchacai, a Xianxiang Xiangchacai \u00e9s a Longye Xiangchacai meg\u00e1llap\u00edtott kontrollt\u00e9rk\u00e9p\u00e9n 19, 24, 26 \u00e9s 21 k\u00f6z\u00f6s cs\u00facsot kalibr\u00e1ltak. A k\u00f6z\u00f6s cs\u00facsok k\u00fcl\u00f6nbs\u00e9gei alapj\u00e1n a n\u00e9gy alapn\u00f6v\u00e9nyt el\u0151zetesen meg lehet k\u00fcl\u00f6nb\u00f6ztetni. A Xianwen Xiangchacai \u00e9s a Longye Xiangchacai begy\u0171jt\u00f6tt t\u00e9teleinek kis sz\u00e1ma miatt, a kutat\u00e1si folyamat hib\u00e1inak elker\u00fcl\u00e9se \u00e9rdek\u00e9ben a k\u00f6z\u00f6s cs\u00facsok vizsg\u00e1latakor a magasabb v\u00e1lasz\u00e9rt\u00e9kekkel, jobb elv\u00e1laszt\u00e1si fokkal \u00e9s cs\u00facsform\u00e1val rendelkez\u0151 kromatogr\u00e1fi\u00e1s cs\u00facsokat v\u00e1lasztottuk ki a kalibr\u00e1l\u00e1shoz. A hasonl\u00f3s\u00e1g\u00e9rt\u00e9kel\u00e9s eredm\u00e9nyei azt mutatj\u00e1k, hogy a sz\u00e1rmaz\u00e1si helybeli k\u00fcl\u00f6nbs\u00e9gek miatt bizonyos k\u00fcl\u00f6nbs\u00e9gek vannak az azonos eredeti gy\u00f3gy\u00e1szati anyagok k\u00f6z\u00f6tt; a k\u00fcl\u00f6nb\u00f6z\u0151 alapgy\u00f3gy\u00e1szati anyagok k\u00f6z\u00f6tt is vannak k\u00fcl\u00f6nbs\u00e9gek, amelyek k\u00f6z\u00fcl a Xihuangcao \u00e9s a m\u00e1sik h\u00e1rom alapgy\u00f3gy\u00e1szati anyag k\u00f6z\u00f6tti hasonl\u00f3s\u00e1g viszonylag alacsony \u00e9s egy\u00e9rtelm\u0171en megk\u00fcl\u00f6nb\u00f6ztethet\u0151; a Xianhua illatos te\u00e1s z\u00f6lds\u00e9g \u00e9s a Xianwen illatos te\u00e1s z\u00f6lds\u00e9g k\u00f6z\u00f6tti hasonl\u00f3s\u00e1g viszonylag magas, \u00e9s a Changye illatos te\u00e1s z\u00f6lds\u00e9g \u00e9s a Xianhua illatos te\u00e1s z\u00f6lds\u00e9g k\u00f6z\u00f6tti hasonl\u00f3s\u00e1g szint\u00e9n viszonylag magas. Ennek oka az lehet, hogy a Xianhua illatos tean\u00f6v\u00e9ny, a Xianwen illatos tean\u00f6v\u00e9ny \u00e9s a Changye illatos tean\u00f6v\u00e9ny mind a r\u00e1ncos level\u0171 illatos tean\u00f6v\u00e9nyek csoportj\u00e1ba tartoz\u00f3 n\u00f6v\u00e9nyek, hasonl\u00f3 k\u00e9miai \u00f6sszet\u00e9tel\u0171 t\u00edpusokkal. A megl\u00e9v\u0151 kutat\u00e1sok kimutatt\u00e1k, hogy a Xihuangcao gy\u00f3gy\u00e1szati anyagok eredeti n\u00f6v\u00e9nyeiben tal\u00e1lhat\u00f3 k\u00e9miai \u00f6sszetev\u0151k t\u00edpusai k\u00f6z\u00f6tt kev\u00e9s k\u00fcl\u00f6nbs\u00e9g van, amelyek f\u0151k\u00e9nt terp\u00e9neket, flavonoidokat, fenolsavakat \u00e9s szterolokat tartalmaznak. Ezek k\u00f6z\u00fcl a diterp\u00e9nek a Xihuangcao gy\u00f3gyn\u00f6v\u00e9ny fontos \u00f6sszetev\u0151i. Az eredeti Xihuangcao n\u00f6v\u00e9nyben tal\u00e1lhat\u00f3 diterp\u00e9nek f\u0151 t\u00edpusa az enantiomer kaempferol t\u00edpus, m\u00edg a line\u00e1ris illatos tean\u00f6v\u00e9ny \u00e9s annak rostos vir\u00e1g\u00fa v\u00e1ltozata, valamint a hossz\u00fa level\u0171 illatos tean\u00f6v\u00e9ny f\u0151k\u00e9nt a gyanta t\u00edpus\u00fa \u00e9s az enantiomer gyanta t\u00edpus\u00fa diterp\u00e9neket tartalmazza. Felt\u00e9telezhet\u0151, hogy a Xihuangcao \u00e9s a line\u00e1ris illatos tean\u00f6v\u00e9ny, a rostos illatos tean\u00f6v\u00e9ny, valamint a hossz\u00fa level\u0171 illatos tean\u00f6v\u00e9ny k\u00f6z\u00f6tti alacsony hasonl\u00f3s\u00e1g a diterp\u00e9nek t\u00edpusainak k\u00fcl\u00f6nbs\u00e9g\u00e9vel f\u00fcgghet \u00f6ssze, m\u00edg a line\u00e1ris illatos tean\u00f6v\u00e9ny, a rostos illatos tean\u00f6v\u00e9ny \u00e9s a hossz\u00fa level\u0171 illatos tean\u00f6v\u00e9ny k\u00f6z\u00f6tti k\u00fcl\u00f6nbs\u00e9gek a megfelel\u0151 \u00f6sszetev\u0151k tartalm\u00e1val \u00e9s t\u00edpus\u00e1val f\u00fcgghetnek \u00f6ssze.<br \/>\nA f\u0151komponens-elemz\u00e9s kimutatta, hogy a Xihuangcao az egyes alapelemek f\u0151komponens-pontsz\u00e1m\u00e1nak k\u00fcl\u00f6nbs\u00e9ge alapj\u00e1n tov\u00e1bb k\u00fcl\u00f6nb\u00f6ztethet\u0151 meg a b\u00e9lelt illatos te\u00e1s z\u00f6lds\u00e9gt\u0151l, az illatos te\u00e1s z\u00f6lds\u00e9gt\u0151l \u00e9s a hossz\u00fa level\u0171 illatos te\u00e1s z\u00f6lds\u00e9gt\u0151l. A r\u00e9szleges legkisebb n\u00e9gyzetek diszkriminanciaanal\u00edzise h\u00e1romf\u00e9le \u0151sn\u00f6v\u00e9nyt, nevezetesen a vonalas mint\u00e1zat\u00fa illatos tean\u00f6v\u00e9nyt, a sz\u00e1las illatos tean\u00f6v\u00e9nyt \u00e9s a hossz\u00fa level\u0171 illatos tean\u00f6v\u00e9nyt k\u00e9pes megk\u00fcl\u00f6nb\u00f6ztetni, jelent\u0151s oszt\u00e1lyoz\u00e1si hat\u00e1sokkal. A kapott 15 oszt\u00e1lyoz\u00e1si marker a f\u0151 jellemz\u0151 komponensek, amelyek k\u00fcl\u00f6nbs\u00e9get okoznak a h\u00e1rom k\u00f6z\u00f6tt, \u00e9s amelyek nagy jelent\u0151s\u00e9ggel b\u00edrnak e h\u00e1rom k\u00fcl\u00f6nb\u00f6z\u0151 primit\u00edv n\u00f6v\u00e9ny megk\u00fcl\u00f6nb\u00f6ztet\u00e9s\u00e9ben. Ennek a 15 kromatogr\u00e1fi\u00e1s cs\u00facsnak az azonos\u00edt\u00e1sa m\u00e9g tov\u00e1bbi kutat\u00e1sokat \u00e9s azonos\u00edt\u00e1st ig\u00e9nyel.<br \/>\n\u00d6sszefoglalva, a Xihuangcao n\u00e9gy alapn\u00f6v\u00e9ny\u00e9nek UPLC jellegzetes t\u00e9rk\u00e9pei, amelyeket ebben a tanulm\u00e1nyban \u00e1ll\u00edtottak fel, a jellegzetes cs\u00facsok kalibr\u00e1l\u00e1sa \u00e9s a hasonl\u00f3s\u00e1g \u00e9rt\u00e9kel\u00e9se r\u00e9v\u00e9n el\u0151zetesen megk\u00fcl\u00f6nb\u00f6ztethetik a n\u00e9gy k\u00fcl\u00f6nb\u00f6z\u0151 alapn\u00f6v\u00e9nyt. Az eredeti n\u00f6v\u00e9nyek n\u00e9gy t\u00edpus\u00e1nak jobb megk\u00fcl\u00f6nb\u00f6ztet\u00e9se \u00e9s a differenci\u00e1lis komponensek keres\u00e9se \u00e9rdek\u00e9ben ez a tanulm\u00e1ny a tov\u00e1bbiakban klaszterelemz\u00e9st, f\u0151komponens-elemz\u00e9st \u00e9s r\u00e9szleges legkisebb n\u00e9gyzetek diszkriminancia-elemz\u00e9st alkalmazott a n\u00e9gy Xihuangcao-i eredeti n\u00f6v\u00e9nyt\u00edpus teljes megk\u00fcl\u00f6nb\u00f6ztet\u00e9s\u00e9re. A kutat\u00e1si eredm\u00e9nyek tudom\u00e1nyos m\u00f3dszert \u00e9s referenci\u00e1t ny\u00fajtanak a Xihuangcao gy\u00f3gy\u00e1szati anyagokban tal\u00e1lhat\u00f3 eredeti n\u00f6v\u00e9nyek azonos\u00edt\u00e1s\u00e1hoz, min\u0151s\u00e9gelemz\u00e9s\u00e9hez \u00e9s \u00e9rt\u00e9kel\u00e9s\u00e9hez.<\/p>","protected":false},"excerpt":{"rendered":"<p>Identification of UPLC characteristic spectra of four original plants of Xihuangcao medicinal herbs Xihuang Grass is a unique Chinese herbal medicine in the south of the Five Ridges, which has the effect of clearing heat and removing dampness, cooling blood and removing stasis. 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