{"id":6545,"date":"2024-08-06T12:29:50","date_gmt":"2024-08-06T12:29:50","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=6545"},"modified":"2024-08-06T12:29:50","modified_gmt":"2024-08-06T12:29:50","slug":"tu-niu-knee","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/hu\/tu-niu-terd\/","title":{"rendered":"A Tu Niu t\u00e9rdb\u0151l sz\u00e1rmaz\u00f3 etanolos kivonat extrakci\u00f3s folyamat\u00e1nak optimaliz\u00e1l\u00e1sa \u00e9s in vitro hum\u00e1n t\u00fcd\u0151 adenokarcin\u00f3ma A549 sejtek elleni aktivit\u00e1s\u00e1nak optimaliz\u00e1l\u00e1sa"},"content":{"rendered":"<p>A Tu Niu t\u00e9rdb\u0151l sz\u00e1rmaz\u00f3 etanolos kivonat extrakci\u00f3s folyamat\u00e1nak optimaliz\u00e1l\u00e1sa \u00e9s in vitro hum\u00e1n t\u00fcd\u0151 adenokarcin\u00f3ma A549 sejtek elleni aktivit\u00e1s\u00e1nak optimaliz\u00e1l\u00e1sa<br \/>\nA k\u00ednai gy\u00f3gyn\u00f6v\u00e9nyk\u00e9nt is ismert Achyranthes aspera L. m\u00e9regtelen\u00edt\u0151, r\u00e1kellenes, kotr\u00e1si \u00e9s vizelethajt\u00f3 hat\u00e1s\u00fa. D\u00e9lnyugat-K\u00edna sz\u00e1mos etnikai kisebbs\u00e9ge, k\u00f6zt\u00fck a miao, yao, zhuang, dai \u00e9s yi etnikai csoportok \u00e1ltal\u00e1nosan haszn\u00e1lj\u00e1k. Az Achyranthes bidentata triterp\u00e9n szaponinokat tartalmaz, amelyek anyamagja az oleanolsav. A hidroliz\u00e1lt oleanolsav antioxid\u00e1ns, gyullad\u00e1scs\u00f6kkent\u0151, \u00e9relmeszesed\u00e9s elleni \u00e9s egy\u00e9b biol\u00f3giai aktivit\u00e1ssal rendelkezik. Funkcion\u00e1lis jellemz\u0151i, p\u00e9ld\u00e1ul a v\u00e9rcukorszint cs\u00f6kkent\u00e9se, antibakteri\u00e1lis, f\u00e1jdalomcsillap\u00edt\u00f3 \u00e9s gyullad\u00e1scs\u00f6kkent\u0151 tulajdons\u00e1gai miatt az Achyranthes bidentata egyed\u00fcl\u00e1ll\u00f3 alkalmaz\u00e1si \u00e9rt\u00e9kkel rendelkezik az orvostudom\u00e1nyban \u00e9s sz\u00e9les k\u00f6r\u0171 fejl\u0151d\u00e9si kil\u00e1t\u00e1sokkal. Ez\u00e9rt az Achyranthes bidentata hat\u00f3anyagainak \u00e9s funkcion\u00e1lis jellemz\u0151inek tanulm\u00e1nyoz\u00e1sa nagy jelent\u0151s\u00e9ggel b\u00edr.<\/p>\n<p>Kor\u00e1bbi kutat\u00f3k 58 fontos anyagot izol\u00e1ltak \u00e9s azonos\u00edtottak az Achyranthes bidentat\u00e1b\u00f3l, amelyek k\u00f6z\u00fcl a f\u0151 izol\u00e1lt \u00e9s nyert komponensek t\u00f6bbnyire poliszacharidok, flavonoidok, terp\u00e9nek, n\u00f6v\u00e9nyi szterolok, szaponinok stb. voltak. Ezek k\u00fcl\u00f6nb\u00f6z\u0151 gy\u00f3gy\u00e1szati tulajdons\u00e1gokkal rendelkeznek, p\u00e9ld\u00e1ul gyullad\u00e1scs\u00f6kkent\u0151, antioxid\u00e1ns, hipoglik\u00e9mi\u00e1s \u00e9s antibakteri\u00e1lis hat\u00e1s\u00faak, \u00edgy sz\u00e9lesk\u00f6r\u0171 fejl\u0151d\u00e9si lehet\u0151s\u00e9gekkel rendelkeznek az orvostudom\u00e1ny ter\u00fclet\u00e9n. Az Achyranthes bidentata vizes \u00e9s alkoholos kivonata egyar\u00e1nt j\u00f3 antibakteri\u00e1lis aktivit\u00e1ssal rendelkezik, de az in vitro r\u00e1kellenes aktivit\u00e1st \u00e9s a klinikai hat\u00e1sokat \u00e9s mechanizmusokat m\u00e9g nem vizsg\u00e1lt\u00e1k. Ez a tanulm\u00e1ny az Eucommia ulmoides etanolos kivonat\u00e1nak kivon\u00e1s\u00e1ra etanolos reflux m\u00f3dszerrel k\u00edv\u00e1nja alkalmazni az Eucommia ulmoidesb\u0151l. Egyetlen t\u00e9nyez\u0151 alapj\u00e1n a v\u00e1laszfel\u00fclet-optimaliz\u00e1l\u00e1si extrakci\u00f3s elj\u00e1r\u00e1st fogadjuk el a hat\u00e9kony \u00e9s minim\u00e1lisan rombol\u00f3 extrakci\u00f3s elj\u00e1r\u00e1s megtal\u00e1l\u00e1sa \u00e9rdek\u00e9ben. Az Eucommia ulmoides etanolos kivonat\u00e1nak az emberi t\u00fcd\u0151 adenokarcin\u00f3ma A549 sejtekre gyakorolt hat\u00e1s\u00e1t el\u0151zetesen tanulm\u00e1nyozzuk sejtszinten, hogy elm\u00e9leti alapot biztos\u00edtsunk az Eucommia ulmoides felhaszn\u00e1l\u00e1s\u00e1hoz \u00e9s az Eucommia ulmoides etanolos kivonat\u00e1nak r\u00e1kellenes funkci\u00f3j\u00e1nak fejleszt\u00e9s\u00e9hez.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-6546\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-237.png\" alt=\"\" width=\"330\" height=\"237\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-6547\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/2-198.png\" alt=\"\" width=\"315\" height=\"227\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-6548\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/3-186.png\" alt=\"\" width=\"332\" height=\"240\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-6549\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/4-165.png\" alt=\"\" width=\"316\" height=\"236\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-6550\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/5-149.png\" alt=\"\" width=\"331\" height=\"199\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-6551\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/6-135.png\" alt=\"\" width=\"328\" height=\"476\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-6552\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/7-123.png\" alt=\"\" width=\"633\" height=\"396\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-6553\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/8-111.png\" alt=\"\" width=\"607\" height=\"732\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/8-111.png 607w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/8-111-597x720.png 597w\" sizes=\"(max-width: 607px) 100vw, 607px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-6554\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/9-103.png\" alt=\"\" width=\"331\" height=\"255\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-6555\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/10-82.png\" alt=\"\" width=\"335\" height=\"194\" \/><br \/>\nA Radix Achyranthesb\u0151l sz\u00e1rmaz\u00f3 hat\u00f3anyagok kivon\u00e1s\u00e1ra etanolos refluxot alkalmaztak, \u00e9s a Radix Achyranthes etanolos kivonat\u00e1nak kivon\u00e1s\u00e1hoz sz\u00fcks\u00e9ges optim\u00e1lis folyamatfelt\u00e9teleket egyt\u00e9nyez\u0151s k\u00eds\u00e9rletekkel \u00e9s v\u00e1laszfel\u00fclet-optimaliz\u00e1l\u00e1ssal hat\u00e1rozt\u00e1k meg. A h\u0151m\u00e9rs\u00e9klet 85,84 \u2103, a folyad\u00e9k-anyag ar\u00e1ny 41,46:1, az extrakci\u00f3s id\u0151 pedig 2,14 \u00f3ra volt. A becs\u00fclt elm\u00e9leti extrakci\u00f3s sebess\u00e9g 9,36% volt. A t\u00e9nyleges m\u0171k\u00f6d\u00e9si felt\u00e9teleket figyelembe v\u00e9ve, 90 \u2103 h\u0151m\u00e9rs\u00e9kletet, 40:1 folyad\u00e9k-anyag ar\u00e1nyt \u00e9s 2 \u00f3ra extrakci\u00f3s id\u0151t alkalmazva, a talaj teh\u00e9ntej t\u00e9rd etanolos kivonat\u00e1nak ezen k\u00f6r\u00fclm\u00e9nyek k\u00f6z\u00f6tt kapott extrakci\u00f3s r\u00e1t\u00e1ja 9,23% volt, ami n\u00e9mileg elt\u00e9r a 9,36% el\u0151rejelzett \u00e9rt\u00e9kt\u0151l. A citotoxicit\u00e1si k\u00eds\u00e9rlet eredm\u00e9nyei azt mutatt\u00e1k, hogy az etanol-kivonat koncentr\u00e1ci\u00f3j\u00e1nak n\u00f6veked\u00e9s\u00e9vel az A549 sejtek t\u00fal\u00e9l\u00e9si ar\u00e1nya gyorsan cs\u00f6kkent. Amikor az etanol-kivonat koncentr\u00e1ci\u00f3ja 256 \u03bc g\/ml volt, az A549 sejtek g\u00e1tl\u00e1si ar\u00e1nya el\u00e9rte a 94,65%-t, \u00e9s az etanol-kivonat r\u00e1kos sejteken v\u00e9gzett karcol\u00e1s gy\u00f3gyul\u00e1si ar\u00e1nya jelent\u0151sen alacsonyabb volt, mint a vak kontrollcsoport\u00e9 (P&lt;0,001), ami azt jelzi, hogy a teh\u00e9nt\u00e9rd etanol-kivonat\u00e1nak van bizonyos r\u00e1kellenes aktivit\u00e1sa. Ezt k\u00f6vet\u0151en olyan technik\u00e1k, mint a g\u00e1zkromatogr\u00e1fia-t\u00f6megspektrometria (GC-MS) \u00e9s a nagy teljes\u00edtm\u00e9ny\u0171 folyad\u00e9kkromatogr\u00e1fia tandem kvadrupol\/time-of-flight t\u00f6megspektrometria (HPLC-Q-TOF\/MS) felhaszn\u00e1lhat\u00f3k a Radix Achyranthes etanol-kivonat\u00e1nak k\u00e9miai \u00f6sszet\u00e9tel\u00e9nek min\u0151s\u00e9gi \u00e9s mennyis\u00e9gi vizsg\u00e1lat\u00e1ra, megk\u00eds\u00e9relve egy teljes min\u0151s\u00e9gellen\u0151rz\u00e9si rendszer l\u00e9trehoz\u00e1s\u00e1t. Ugyanakkor a Radix Achyranthes kivonat hat\u00e1s\u00e1t az A549-es meztelen eg\u00e9r A549-es t\u00fcd\u0151r\u00e1ksejt-transzplant\u00e1ci\u00f3s tumormodellre is meg lehet vizsg\u00e1lni, hogy tov\u00e1bb vizsg\u00e1ljuk a r\u00e1kellenes mechanizmus\u00e1t.<\/p>","protected":false},"excerpt":{"rendered":"<p>Optimization of Extraction Process and In vitro Anti human Lung Adenocarcinoma A549 Cell Activity of Ethanol Extract from Tu Niu Knee Achyranthes aspera L., also known as Chinese medicinal herb, has the effects of detoxification, anti-cancer, dredging, and diuresis. It is commonly used by many ethnic minorities in southwestern China, including the Miao, Yao, Zhuang, [&hellip;]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.0 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Optimization of Extraction Process and In vitro Anti human Lung Adenocarcinoma A549 Cell Activity of Ethanol Extract from Tu Niu Knee - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/hu\/tu-niu-terd\/\" \/>\n<meta property=\"og:locale\" content=\"hu_HU\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Optimization of Extraction Process and In vitro Anti human Lung Adenocarcinoma A549 Cell Activity of Ethanol Extract from Tu Niu Knee - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"Optimization of Extraction Process and In vitro Anti human Lung Adenocarcinoma A549 Cell Activity of Ethanol Extract from Tu Niu Knee Achyranthes aspera L., also known as Chinese medicinal herb, has the effects of detoxification, anti-cancer, dredging, and diuresis. 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