{"id":5716,"date":"2024-08-04T20:50:17","date_gmt":"2024-08-04T20:50:17","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=5716"},"modified":"2024-08-04T20:50:17","modified_gmt":"2024-08-04T20:50:17","slug":"bombyx-mori-medicinal-materials","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/hu\/bombyx-mori-medicinal-materials\/","title":{"rendered":"Feh\u00e9rjeoszt\u00e1lyoz\u00e1si ujjlenyomat-t\u00e9rk\u00e9p l\u00e9trehoz\u00e1sa \u00e9s molekul\u00e1ris vizsg\u00e1lat a Bombyx mori gy\u00f3gy\u00e1szati anyagok eredet\u00e9nek azonos\u00edt\u00e1s\u00e1ra"},"content":{"rendered":"

Feh\u00e9rjeoszt\u00e1lyoz\u00e1si ujjlenyomat-t\u00e9rk\u00e9p l\u00e9trehoz\u00e1sa \u00e9s molekul\u00e1ris vizsg\u00e1lat a Bombyx mori gy\u00f3gy\u00e1szati anyagok eredet\u00e9nek azonos\u00edt\u00e1s\u00e1ra
\nA Bombyx Batryticatus a Beauveria bassiana (Bals.) Vuillart selyemherny\u00f3 l\u00e1rv\u00e1k teny\u00e9szt\u00e9s k\u00f6zbeni fert\u0151z\u00e9se (vagy mesters\u00e9ges beolt\u00e1sa) sor\u00e1n keletkez\u0151 mell\u00e9kterm\u00e9k, amely elhal\u00e1st okoz. Sz\u00e9l- \u00e9s g\u00f6rcsold\u00f3, sz\u00e9l- \u00e9s f\u00e1jdalomcsillap\u00edt\u00f3, valamint flegma- \u00e9s csom\u00f3old\u00f3 hat\u00e1s\u00fa. Az \"eperfa keletr\u0151l nyugatra t\u00f6rt\u00e9n\u0151 \u00e1thelyez\u00e9se\" ipari elrendez\u00e9s\u00e9nek K\u00edn\u00e1ban t\u00f6rt\u00e9n\u0151 megval\u00f3s\u00edt\u00e1sa miatt Szecsu\u00e1n \u00e9s J\u00fcnnan v\u00e1ltak a sarjadz\u00f3ipar f\u0151 tartom\u00e1nyaiv\u00e1, \u00e9s \u00edgy a selyemherny\u00f3 gy\u00f3gy\u00e1szati anyagainak f\u0151 term\u0151ter\u00fcleteiv\u00e9. Mivel a Szecsu\u00e1nban termelt selyemherny\u00f3k jobb morfol\u00f3giai jellemz\u0151kkel rendelkeznek, mint a Junanban termelt selyemherny\u00f3k, amelyek megfelelnek a kor\u00e1bbi k\u00ednai gy\u00f3gyn\u00f6v\u00e9nyk\u00f6nyvek \u00e9s gy\u00f3gyszerk\u00f6nyvek 63. \u00e9s 77. kiad\u00e1s\u00e1ban le\u00edrt \"egyenes \u00e9s vastag test, szil\u00e1rd text\u00fara, feh\u00e9r sz\u00edn \u00e9s vil\u00e1gos keresztmetszet\" min\u0151s\u00e9gi selyemherny\u00f3-szabv\u00e1nyoknak, piaci elismerts\u00e9g\u00fck nagyobb, \u00e9s elad\u00e1si \u00e1ruk is magasabb. A piackutat\u00e1s sor\u00e1n meg\u00e1llap\u00edtott\u00e1k, hogy a keresked\u0151k gyakran sz\u00e1nd\u00e9kosan \u00f6sszekeverik a k\u00e9t term\u00e9k eredet\u00e9t, hogy hasznot h\u00fazzanak az elad\u00e1saikb\u00f3l. A klinikai gy\u00f3gyszerek biztons\u00e1g\u00e1nak \u00e9s hat\u00e9konys\u00e1g\u00e1nak jav\u00edt\u00e1sa \u00e9rdek\u00e9ben kutat\u00e1st kell v\u00e9gezni a selyemherny\u00f3k eredet\u00e9nek azonos\u00edt\u00e1si m\u00f3dszer\u00e9r\u0151l.<\/p>\n

Mint ismeretes, a k\u00ednai gy\u00f3gy\u00e1szati anyagok hat\u00e9kony \u00f6sszetev\u0151inek kialakul\u00e1sa \u00e9s felhalmoz\u00f3d\u00e1sa szorosan \u00f6sszef\u00fcgg azok term\u00e9szetes n\u00f6veked\u00e9si k\u00f6r\u00fclm\u00e9nyeivel. Ez\u00e9rt az eredet az egyik fontos t\u00e9nyez\u0151, amely befoly\u00e1solja a k\u00ednai gy\u00f3gy\u00e1szati anyagok min\u0151s\u00e9g\u00e9t. Az eredetazonos\u00edt\u00e1si \u00e9s nyomonk\u00f6vethet\u0151s\u00e9gi m\u00f3dszerek kidolgoz\u00e1sa fontos szerepet j\u00e1tszik a fogyaszt\u00f3k term\u00e9kinform\u00e1ci\u00f3k meg\u00e9rt\u00e9s\u00e9re vonatkoz\u00f3 ig\u00e9nyeinek kiel\u00e9g\u00edt\u00e9s\u00e9ben, a termel\u0151, feldolgoz\u00f3 \u00e9s \u00e9rt\u00e9kes\u00edt\u0151 v\u00e1llalkoz\u00e1sok jogainak \u00e9s \u00e9rdekeinek v\u00e9delm\u00e9ben, valamint a k\u00ednai gy\u00f3gy\u00e1szati anyagok versenyk\u00e9pess\u00e9g\u00e9nek fokoz\u00e1s\u00e1ban a nemzetk\u00f6zi piacon. Liao Baosheng \u00e9s m\u00e1sok azt javasolt\u00e1k, hogy a k\u00ednai gy\u00f3gy\u00e1szati anyagok eredetazonos\u00edt\u00e1sa \u00e9s nyomon k\u00f6vethet\u0151s\u00e9ge a molekul\u00e1ris biol\u00f3giai technol\u00f3gia, a hagyom\u00e1nyos k\u00ednai gy\u00f3gy\u00e1szati ujjlenyomat-technol\u00f3gia, az izot\u00f3pos nyomk\u00f6vet\u0151 technol\u00f3gia, a vezet\u00e9k n\u00e9lk\u00fcli r\u00e1di\u00f3frekvenci\u00e1s azonos\u00edt\u00f3 nyomon k\u00f6vet\u00e9si technol\u00f3gia \u00e9s a vonalk\u00f3d-technol\u00f3gia alapj\u00e1n v\u00e9gezhet\u0151 el. A feh\u00e9rjeelektrofor\u00e9zis a Nemzetk\u00f6zi Vet\u0151mag-ellen\u0151rz\u00e9si Sz\u00f6vets\u00e9g \u00e1ltal elfogadott szabv\u00e1nyos fajtaazonos\u00edt\u00e1si m\u00f3dszer, de ennek a m\u00f3dszernek m\u00e9g mindig h\u00e1tr\u00e1nya az alacsony felbont\u00e1s \u00e9s a fajon bel\u00fcli azonos\u00edt\u00e1s neh\u00e9zs\u00e9ge, ami megnehez\u00edti a hagyom\u00e1nyos k\u00ednai gy\u00f3gy\u00e1szati anyagok eredetazonos\u00edt\u00e1s\u00e1t \u00e9s nyomon k\u00f6vethet\u0151s\u00e9g\u00e9t. Ennek f\u00e9ny\u00e9ben ez a cikk a hierarchikus kicsap\u00e1si \u00e9s feh\u00e9rjeelektrofor\u00e9zis technik\u00e1k kombin\u00e1ci\u00f3j\u00e1nak megval\u00f3s\u00edthat\u00f3s\u00e1g\u00e1t k\u00edv\u00e1nja megvizsg\u00e1lni egy hierarchikus ujjlenyomat-technol\u00f3gia l\u00e9trehoz\u00e1sa \u00e9rdek\u00e9ben, hogy elemezze a Yunnan \u00e9s Sichuan selyemherny\u00f3k proteomj\u00e1nak k\u00fcl\u00f6nbs\u00e9geit, azonos\u00edtsa a kapott differenci\u00e1lis molekul\u00e1kat, \u00e9s bioinformatikai elemz\u00e9st v\u00e9gezzen az eredetazonos\u00edt\u00e1s szempontj\u00e1b\u00f3l relev\u00e1ns molekul\u00e1k felt\u00e1r\u00e1s\u00e1ra, k\u00eds\u00e9rleti alapot biztos\u00edtva egy egyszer\u0171, megval\u00f3s\u00edthat\u00f3 \u00e9s tudom\u00e1nyosan pontos eredetazonos\u00edt\u00e1si m\u00f3dszer l\u00e9trehoz\u00e1s\u00e1hoz.<\/p>\n

\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"Ebben a cikkben Yunnan \u00e9s Sichuan selyemherny\u00f3ib\u00f3l savban, l\u00fagban, v\u00edzben \u00e9s alkoholban old\u00f3d\u00f3 feh\u00e9rj\u00e9ket vontak ki, \u00e9s megvizsg\u00e1lt\u00e1k az \u00f6sszes feh\u00e9rje \u00e9s az oszt\u00e1lyozott ujjlenyomat spektrum\u00e1t. Az eredm\u00e9nyek azt mutatt\u00e1k, hogy a k\u00e9t selyemherny\u00f3 teljes feh\u00e9rje ujjlenyomat spektruma nagyon hasonl\u00f3, ami megnehez\u00edti a sz\u00e1rmaz\u00e1s azonos\u00edt\u00e1s\u00e1ra val\u00f3 felhaszn\u00e1l\u00e1sukat; A k\u00e9t selyemherny\u00f3 faj k\u00f6z\u00f6tt jelent\u0151s k\u00fcl\u00f6nbs\u00e9g van az oszt\u00e1lyozott ujjlenyomat spektrum\u00e1ban, ami v\u00e1rhat\u00f3an felhaszn\u00e1lhat\u00f3 a sz\u00e1rmaz\u00e1s azonos\u00edt\u00e1s\u00e1ra; A hierarchikus ujjlenyomat spektrum \u00e1ltal ny\u00fajtott inform\u00e1ci\u00f3 gazdags\u00e1ga az alkoholban old\u00f3d\u00f3 feh\u00e9rj\u00e9k>savban old\u00f3d\u00f3 feh\u00e9rj\u00e9k>v\u00edzben old\u00f3d\u00f3 feh\u00e9rj\u00e9k>l\u00fagban old\u00f3d\u00f3 feh\u00e9rj\u00e9k>l\u00fagban old\u00f3d\u00f3 feh\u00e9rj\u00e9k sorrendj\u00e9ben van. A hierarchikus ujjlenyomat-t\u00e9rk\u00e9pek k\u00fcl\u00f6nbs\u00e9gei alapj\u00e1n \u00f6sszesen 273 jellegzetes feh\u00e9rjemolekul\u00e1t fedeztek fel, k\u00f6zt\u00fck \u03b2-gl\u00fckozid\u00e1z, anti-kimotripszin, anti-tripszin \u00e9s lip\u00e1z dom\u00e9n feh\u00e9rj\u00e9ket. Ezek a molekul\u00e1k olyan aktivit\u00e1sokkal rendelkeznek, mint a transzfer\u00e1z, katal\u00e1z \u00e9s tioredoxin-peroxid\u00e1z, \u00e9s r\u00e9szt vesznek k\u00fcl\u00f6nb\u00f6z\u0151 sejtfolyamatokban, anyagcsere-folyamatokban, ingerekre adott v\u00e1laszokban, biol\u00f3giai szab\u00e1lyoz\u00e1sban \u00e9s fejl\u0151d\u00e9si folyamatokban. Olyan \u00fatvonalakat vonnak be, mint a m\u00e1sodlagos metabolitok szint\u00e9zise, a mikrobi\u00e1lis anyagcsere k\u00fcl\u00f6nb\u00f6z\u0151 k\u00f6rnyezetben, az antibiotikum-bioszint\u00e9zis \u00e9s a biol\u00f3giai v\u00e9dekez\u00e9si mechanizmusok, ami t\u00fckr\u00f6zi a k\u00fcl\u00f6nb\u00f6z\u0151 \u00e9l\u0151helyi t\u00e9nyez\u0151k fontos befoly\u00e1s\u00e1t a selyemherny\u00f3k \u00e9s a Beauveria bassiana k\u00f6z\u00f6tti k\u00f6lcs\u00f6nhat\u00e1sra \u00e9s a Beauveria bassiana kialakul\u00e1s\u00e1ra. Ezek potenci\u00e1lis \u00e9rt\u00e9kkel b\u00edrnak a Beauveria bassiana eredet\u00e9nek azonos\u00edt\u00e1s\u00e1ban.
\nAz autentikus gy\u00f3gyn\u00f6v\u00e9nyek a hagyom\u00e1nyos k\u00ednai gy\u00f3gy\u00e1szatban a gy\u00f3gyn\u00f6v\u00e9nyek min\u0151s\u00e9g\u00e9nek ellen\u0151rz\u00e9s\u00e9re szolg\u00e1l\u00f3 egyed\u00fcl\u00e1ll\u00f3 \u00e1tfog\u00f3 krit\u00e9riumot jelentenek, amely m\u00e9lyen t\u00fckr\u00f6zi a k\u00ednai gy\u00f3gyn\u00f6v\u00e9nyek min\u0151s\u00e9ge \u00e9s sz\u00e1rmaz\u00e1si hely\u00fck k\u00f6z\u00f6tti szoros kapcsolatot. Az eredetazonos\u00edt\u00e1s tudom\u00e1nyos m\u00f3dszer\u00e9nek kidolgoz\u00e1sa fontos technikai t\u00e1mogat\u00e1st ny\u00fajthat a k\u00ednai gy\u00f3gyn\u00f6v\u00e9nypiac fel\u00fcgyelet\u00e9hez. Yang Jian, Zhang Hongkun, Song Jun \u00e9s m\u00e1sok a Dendrobium officinale, Anemarrhena chinensis \u00e9s Auricularia auricula eredetazonos\u00edt\u00e1si vizsg\u00e1latokat v\u00e9geztek stabil izot\u00f3pok, \u00e1sv\u00e1nyi elemek ujjlenyomatai \u00e9s DNS-vonalk\u00f3dok alapj\u00e1n. A molekul\u00e1ris farmakogn\u00f3ziai vizsg\u00e1latok kimutatt\u00e1k, hogy a sz\u00e1rmaz\u00e1si hely hat\u00e1sa a hagyom\u00e1nyos k\u00ednai gy\u00f3gy\u00e1szat min\u0151s\u00e9g\u00e9re az \u00e9l\u0151helyi t\u00e9nyez\u0151k - p\u00e9ld\u00e1ul a h\u0151m\u00e9rs\u00e9klet, a p\u00e1ratartalom \u00e9s a f\u00e9ny - v\u00e1ltoz\u00e1sain kereszt\u00fcl \u00e9rv\u00e9nyes\u00fcl, amelyek befoly\u00e1solj\u00e1k a kapcsol\u00f3d\u00f3 g\u00e9nek kifejez\u0151d\u00e9s\u00e9t. A feh\u00e9rj\u00e9k a g\u00e9nexpresszi\u00f3 term\u00e9kei, \u00e9s az \u00e9l\u0151helyi t\u00e9nyez\u0151k v\u00e1ltoz\u00e1sai elker\u00fclhetetlen\u00fcl befoly\u00e1solj\u00e1k az expresszi\u00f3s t\u00edpusukat \u00e9s a mennyis\u00e9g\u00fcket. Ez\u00e9rt \u00e9rdemes figyelmet ford\u00edtani az eredetazonos\u00edt\u00e1sban val\u00f3 alkalmaz\u00e1suk kil\u00e1t\u00e1saira. E tanulm\u00e1ny eredm\u00e9nyei azt mutatj\u00e1k, hogy a k\u00fcl\u00f6nb\u00f6z\u0151 \u00e9l\u0151helyi t\u00e9nyez\u0151k Yunnanban \u00e9s Sichuanban jelent\u0151s hat\u00e1ssal vannak a k\u00e9t r\u00e9gi\u00f3ban termelt selyemherny\u00f3k feh\u00e9rjeexpresszi\u00f3j\u00e1ra, \u00e9s az ennek megfelel\u0151 k\u00fcl\u00f6nbs\u00e9gek hierarchikus ujjlenyomatok seg\u00edts\u00e9g\u00e9vel kimutathat\u00f3k. Az SDS-PAGE \u00e9s a k\u00e9tdimenzi\u00f3s g\u00e9lelektrofor\u00e9zissel \u00f6sszehasonl\u00edtva a hierarchikus ujjlenyomat-technol\u00f3gia el\u0151sz\u00f6r acetonnal kezeli a mint\u00e1kat, hogy hidrof\u00f3bs\u00e1guk szerint oszt\u00e1lyozza \u0151ket, majd \u00f6sszehasonl\u00edtja a hasonl\u00f3 mint\u00e1kat ugyanazon a g\u00e9len. El\u0151nye az egyszer\u0171 m\u0171k\u00f6d\u00e9s, a nagy felbont\u00e1s, a pontos eredm\u00e9nyek \u00e9s a nagy reproduk\u00e1lhat\u00f3s\u00e1g, ami referenci\u00e1t biztos\u00edt m\u00e1s k\u00ednai gy\u00f3gy\u00e1szati anyagok eredet\u00e9nek azonos\u00edt\u00e1s\u00e1hoz. Ezenk\u00edv\u00fcl a Bombyx mori alkoholban old\u00f3d\u00f3 feh\u00e9rj\u00e9iben nincsenek jelent\u0151sen nagy abundanci\u00e1j\u00fa molekul\u00e1k. A hierarchikus ujjlenyomat fel\u00e9p\u00edt\u00e9sekor elker\u00fclhet\u0151 a s\u00e1vok k\u00f6z\u00f6tti k\u00f6lcs\u00f6n\u00f6s interferencia \u00e9s lefedetts\u00e9g, \u00edgy gazdagabb ujjlenyomatinform\u00e1ci\u00f3t \u00e9s jobb felbont\u00e1st biztos\u00edt, mint m\u00e1s t\u00edpus\u00fa feh\u00e9rj\u00e9k. A fenti eredm\u00e9nyek m\u00e1s k\u00ednai gy\u00f3gy\u00e1szati anyagok eredet\u00e9nek azonos\u00edt\u00e1s\u00e1hoz kapcsol\u00f3d\u00f3 molekul\u00e1k kiv\u00e1laszt\u00e1s\u00e1hoz is \u00f6tleteket adnak.<\/p>","protected":false},"excerpt":{"rendered":"

Establishment of Protein Grading Fingerprint Map and Molecular Study on Origin Identification of Bombyx mori Medicinal Materials Bombyx Batryticatus is a byproduct formed by the infection (or artificial inoculation) of Beauveria bassiana (Bals.) Vuillart in silkworm larvae during breeding, which causes death. It has the effects of relieving wind and spasms, dispelling wind and pain, […]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"\nEstablishment of Protein Grading Fingerprint Map and Molecular Study on Origin Identification of Bombyx mori Medicinal Materials - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/hu\/bombyx-mori-medicinal-materials\/\" \/>\n<meta property=\"og:locale\" content=\"hu_HU\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Establishment of Protein Grading Fingerprint Map and Molecular Study on Origin Identification of Bombyx mori Medicinal Materials - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"Establishment of Protein Grading Fingerprint Map and Molecular Study on Origin Identification of Bombyx mori Medicinal Materials Bombyx Batryticatus is a byproduct formed by the infection (or artificial inoculation) of Beauveria bassiana (Bals.) Vuillart in silkworm larvae during breeding, which causes death. 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