{"id":5686,"date":"2024-08-04T20:41:54","date_gmt":"2024-08-04T20:41:54","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=5686"},"modified":"2024-08-04T20:41:54","modified_gmt":"2024-08-04T20:41:54","slug":"dosage-values-of-bu-zha-ye","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/hu\/dosage-values-of-bu-zha-ye\/","title":{"rendered":"Tanulm\u00e1ny a Bu Zha Ye f\u0151zetdarabok \u00e9s standard f\u0151zetek d\u00f3zis\u00e9rt\u00e9keinek \u00e1tad\u00e1s\u00e1r\u00f3l UPLC ujjlenyomat-spektrum \u00e9s t\u00f6bbkomponens\u0171 mennyis\u00e9gi meghat\u00e1roz\u00e1s alapj\u00e1n"},"content":{"rendered":"

Tanulm\u00e1ny a Bu Zha Ye f\u0151zetdarabok \u00e9s standard f\u0151zetek d\u00f3zis\u00e9rt\u00e9keinek \u00e1tad\u00e1s\u00e1r\u00f3l UPLC ujjlenyomat-spektrum \u00e9s t\u00f6bbkomponens\u0171 mennyis\u00e9gi meghat\u00e1roz\u00e1s alapj\u00e1n
\nA Bu Zha Ye egy jellegzetes gy\u00f3gyn\u00f6v\u00e9ny Lingnanban, amely f\u0151k\u00e9nt flavonoidokb\u00f3l, alkaloidokb\u00f3l, terp\u00e9nekb\u0151l, szerves savakb\u00f3l stb. \u00e1ll. A gyomor-b\u00e9lrendszeri perisztaltika \u00e9s a gyomornedv kiv\u00e1laszt\u00e1s\u00e1nak el\u0151seg\u00edt\u00e9se, a v\u00e9rzs\u00edrok cs\u00f6kkent\u00e9se, a s\u00e1rgas\u00e1g cs\u00f6kkent\u00e9se, gyullad\u00e1scs\u00f6kkent\u0151, f\u00e1jdalomcsillap\u00edt\u00f3, sz\u00edv- \u00e9s \u00e9rrendszeri v\u00e9delem, \u00f6reged\u00e9sg\u00e1tl\u00f3 stb. hat\u00e1sai. Ez egy kett\u0151s c\u00e9l\u00fa k\u00ednai gy\u00f3gyszer mind a gy\u00f3gy\u00e1szatban, mind az \u00e9lelmiszerekben. Az ut\u00f3bbi \u00e9vekben megn\u0151tt a Bu Zha Ye ir\u00e1nti figyelem, \u00e9s a vele kapcsolatos kutat\u00e1sok is egyre kiterjedtebb\u00e9 v\u00e1ltak.
\nA hagyom\u00e1nyos k\u00ednai orvosl\u00e1s f\u0151leg f\u0151zeteket haszn\u00e1l f\u0151 gy\u00f3gyszerformak\u00e9nt, amelyek a k\u00ednai orvosl\u00e1s hat\u00e9konys\u00e1g\u00e1nak anyagi alapj\u00e1t k\u00e9pezik. T\u00f6bb ezer \u00e9ves klinikai gyakorlat ut\u00e1n a k\u00ednai orvosl\u00e1s standard f\u0151zeteire vonatkoz\u00f3 min\u0151s\u00e9gi szabv\u00e1nyok meg\u00e1llap\u00edt\u00e1sa fontos hivatkoz\u00e1si alapot ny\u00fajthat a modern k\u00ednai gy\u00f3gyszerk\u00e9sz\u00edtm\u00e9nyek, p\u00e9ld\u00e1ul a recept\u00faraszer\u0171 granul\u00e1tumok min\u0151s\u00e9gi szabv\u00e1nyainak meg\u00e1llap\u00edt\u00e1s\u00e1hoz. Jelenleg nincsenek kutat\u00e1si jelent\u00e9sek a Bu Zha Ye standard f\u0151zet\u00e9r\u0151l. C\u00e9g\u00fcnk a Bu Zha Ye formula granul\u00e1tum nemzeti szabv\u00e1ny\u00e1ra vonatkoz\u00f3 kutat\u00e1s\u00e1ban a \"A hagyom\u00e1nyos k\u00ednai orvosl\u00e1s formula granul\u00e1tumainak min\u0151s\u00e9gellen\u0151rz\u00e9s\u00e9re \u00e9s szabv\u00e1nyfejleszt\u00e9s\u00e9re vonatkoz\u00f3 m\u0171szaki k\u00f6vetelm\u00e9nyek\" (a tov\u00e1bbiakban: m\u0171szaki k\u00f6vetelm\u00e9nyek) szerint tanulm\u00e1nyoztuk a standard f\u0151zet kulcsfontoss\u00e1g\u00fa min\u0151s\u00e9gi mutat\u00f3it, bele\u00e9rtve a paszta hozam\u00e1t, a kivonatot, az indik\u00e1tor \u00f6sszetev\u0151k tartalm\u00e1t \u00e9s \u00e1tviteli ar\u00e1ny\u00e1t, az ujjlenyomat spektrum\u00e1t, hogy megvizsg\u00e1ljuk a Bu Zha Ye f\u0151zetdarabokb\u00f3l a standard f\u0151zetbe t\u00f6rt\u00e9n\u0151 mennyis\u00e9gi \u00e9rt\u00e9kek \u00e1tviteli t\u00f6rv\u00e9ny\u00e9t, referenci\u00e1t biztos\u00edtva a Bu Zha Ye formula granul\u00e1tumokra \u00e9s a kapcsol\u00f3d\u00f3 k\u00e9sz\u00edtm\u00e9nyekre vonatkoz\u00f3 min\u0151s\u00e9gi szabv\u00e1nyok kidolgoz\u00e1s\u00e1hoz.<\/p>\n

\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"A Bu Zha Ye min\u0151s\u00e9ge viszonylag k\u00f6nny\u0171, de k\u00e9miai \u00f6sszet\u00e9tele \u00f6sszetett. A f\u0151z\u00e9si folyamat sor\u00e1n nagy mennyis\u00e9g\u0171 \u00f6sszetev\u0151 \u00f6mlik \u00e1t, ami t\u00f6bb szennyez\u0151d\u00e9st eredm\u00e9nyez a standard f\u0151zetben, ami befoly\u00e1solja a hat\u00e9kony \u00f6sszetev\u0151k kromatogr\u00e1fi\u00e1s elv\u00e1laszt\u00e1s\u00e1t \u00e9s elemz\u00e9s\u00e9t. Ha nagy polarit\u00e1s\u00fa old\u00f3szereket, p\u00e9ld\u00e1ul metanolt \u00e9s etanolt haszn\u00e1lunk a standard f\u0151zet kezel\u00e9s\u00e9hez, az ujjlenyomat spektrum alapvonala instabil, a kromatogr\u00e1fi\u00e1s cs\u00facsok elv\u00e1laszt\u00e1sa gyenge, a kromatogr\u00e1fi\u00e1s cs\u00facsok rendezetlenek, \u00e9s sok kis cs\u00facs van. Ez\u00e9rt a standard f\u0151zet vizsg\u00e1lati mint\u00e1k el\u0151kezel\u00e9si m\u00f3dszer\u00e9ben az etil-acet\u00e1t extrakci\u00f3 elv\u00e1laszt\u00e1si, tiszt\u00edt\u00e1si \u00e9s d\u00fas\u00edt\u00e1si m\u00f3dszer\u00e9t vizsg\u00e1lt\u00e1k a szennyez\u0151d\u00e9sek interferenci\u00e1j\u00e1nak cs\u00f6kkent\u00e9se \u00e9rdek\u00e9ben, \u00e9s \u00f6sszehasonl\u00edtott\u00e1k a hagyom\u00e1nyos kezel\u00e9si m\u00f3dszerekkel. Az eredm\u00e9nyek azt mutatt\u00e1k, hogy etil-acet\u00e1tot haszn\u00e1lva extrakci\u00f3s old\u00f3szerk\u00e9nt jelent\u0151sen jav\u00edthat\u00f3 a szennyez\u0151d\u00e9s-cs\u00facsok hat\u00e1sa, biztos\u00edthat\u00f3 a f\u0151 kromatogr\u00e1fi\u00e1s cs\u00facsok tisztas\u00e1ga \u00e9s elv\u00e1laszt\u00e1sa, \u00e9s megoldhat\u00f3 az instabil kromatogr\u00e1fi\u00e1s alapvonal \u00e9s egyes kromatogr\u00e1fi\u00e1s cs\u00facsok kis abszorpci\u00f3j\u00e1nak probl\u00e9m\u00e1ja. A kromatogr\u00e1fi\u00e1s felt\u00e9telek vizsg\u00e1lata sor\u00e1n k\u00fcl\u00f6nb\u00f6z\u0151 mobilf\u00e1zis\u00fa el\u00faci\u00f3s rendszereket vizsg\u00e1ltunk, mint p\u00e9ld\u00e1ul acetonitril-0,1% foszforsav oldat, acetonitril-0,1% ecetsav oldat, metanol-0,1% hangyasav oldat, metanol-0,1% ecetsav oldat stb. Az eredm\u00e9nyek azt mutatt\u00e1k, hogy a metanol-0,1%-ecetsavoldat el\u00faci\u00f3s rendszerk\u00e9nt val\u00f3 alkalmaz\u00e1sa nagyobb sz\u00e1m\u00fa kromatogr\u00e1fi\u00e1s cs\u00facsot \u00e9s jobb \u00e1ltal\u00e1nos elv\u00e1laszt\u00e1st eredm\u00e9nyezett. Tekintettel az ultranagy teljes\u00edtm\u00e9ny\u0171 folyad\u00e9kkromatogr\u00e1fi\u00e1s ujjlenyomat-spektrumok l\u00e9trehoz\u00e1s\u00e1ra, a kromatogr\u00e1fi\u00e1s oszlopok kiv\u00e1laszt\u00e1sa kulcsfontoss\u00e1g\u00fa. Ez\u00e9rt ebben a k\u00eds\u00e9rletben k\u00fcl\u00f6nb\u00f6z\u0151 szemcsem\u00e9ret\u0171 \u00e9s m\u00e1rk\u00e1j\u00fa kromatogr\u00e1fi\u00e1s oszlopokat vizsg\u00e1ltunk, \u00e9s v\u00e9g\u00fcl az Agilent ZORBAX SB C18 kromatogr\u00e1fi\u00e1s oszlopot (2,1 mm \u00d7 150 mm, 1,8 \u03bc m) v\u00e1lasztottuk, amely a legjobb cs\u00facsform\u00e1t \u00e9s felbont\u00e1st biztos\u00edtotta az egyes k\u00f6z\u00f6s ujjlenyomat-cs\u00facsok sz\u00e1m\u00e1ra.
\nA kutat\u00e1sok kimutatt\u00e1k, hogy a Bu Zha levelei sz\u00e1mos flavonoidot tartalmaznak. Xiao \u00e9s munkat\u00e1rsai sikeresen azonos\u00edtottak 31 vegy\u00fcletet a Bu Zha Ye-b\u00f3l UPLC-Q-TOF-MS\/MS technol\u00f3gi\u00e1val, k\u00f6zt\u00fck 28 flavonoidot \u00e9s 3 fenolsavat. Farmakol\u00f3giai vizsg\u00e1latok kimutatt\u00e1k, hogy a flavonoidok fontos anyagi alapj\u00e1t k\u00e9pezik a Bu Zha Ye em\u00e9szt\u00e9st, em\u00e9szt\u00e9st, h\u0151tiszt\u00edt\u00e1st \u00e9s nedvess\u00e9get enyh\u00edt\u0151 hat\u00e1s\u00e1nak. Az olyan reprezentat\u00edv \u00f6sszetev\u0151k, mint a vitexin, az izovitexin \u00e9s a narcissin magas tartalommal rendelkeznek a Bu Zha Ye-ban, \u00e9s bizonyos v\u00edzben val\u00f3 oldhat\u00f3s\u00e1ggal. Ez\u00e9rt a Bu Zha Ye standard f\u0151zet a vitexint, az izovitexint \u00e9s a n\u00e1rcissint haszn\u00e1lja tartalom-meghat\u00e1roz\u00e1si mutat\u00f3k\u00e9nt, \u00e9s a Bu Zha Ye f\u0151zetdarabok tartalom-meghat\u00e1roz\u00e1si m\u00f3dszer\u00e9t a f\u0151zet alapj\u00e1n \u00e1llap\u00edtja meg, hogy ir\u00e1ny\u00edtsa a standard f\u0151zet\u00e9rt\u00e9kek \u00e1tad\u00e1s\u00e1nak tanulm\u00e1nyoz\u00e1s\u00e1t.
\nA Bu Zha Ye gy\u00f3gyn\u00f6v\u00e9nyeket els\u0151sorban Guangdongban, Guangxiban \u00e9s Hainanban terjesztik, \u00e9s a Guangdongban termesztett hiteles gy\u00f3gyn\u00f6v\u00e9nyek k\u00f6z\u00e9 tartoznak. A Bu Zha Ye gy\u00f3gyn\u00f6v\u00e9nyek 15 t\u00e9tel\u00e9t gy\u0171jt\u00f6tt\u00e9k be a h\u00e1rom fent eml\u00edtett term\u0151ter\u00fcletr\u0151l, ami kell\u0151 reprezentativit\u00e1st mutat. A 15 t\u00e9tel standard f\u0151zetkivonat, kivonat \u00e9s 15 t\u00e9tel Bu Zha Ye f\u0151zetdarab h\u00e1rom flavonoidra vonatkoz\u00f3 meghat\u00e1roz\u00e1si eredm\u00e9nyei alapj\u00e1n l\u00e1that\u00f3, hogy a k\u00fcl\u00f6nb\u00f6z\u0151 t\u00e9telekb\u0151l sz\u00e1rmaz\u00f3 mint\u00e1k k\u00f6z\u00f6tt bizonyos k\u00fcl\u00f6nbs\u00e9gek vannak, a maxim\u00e1lis \u00e9s minim\u00e1lis \u00e9rt\u00e9kek k\u00f6r\u00fclbel\u00fcl k\u00e9tszeresen k\u00fcl\u00f6nb\u00f6znek. Ez a k\u00fcl\u00f6nbs\u00e9g maguknak a gy\u00f3gy\u00e1szati anyagoknak a min\u0151s\u00e9g\u00e9vel f\u00fcgg \u00f6ssze, \u00e9s megfelel a Technikai K\u00f6vetelm\u00e9nyek k\u00f6vetelm\u00e9nyeinek. Nincsenek nyilv\u00e1nval\u00f3an rendellenes \u00e9rt\u00e9kek.
\nAz \u00e1tviteli sebess\u00e9g meghat\u00e1roz\u00e1s\u00e1nak eredm\u00e9nyei alapj\u00e1n a h\u00e1rom flavonoid \u00e1tlagos \u00e1tviteli sebess\u00e9ge 40% \u00e9s 60% k\u00f6z\u00f6tt mozgott, a vitexin\u00e9 volt a legmagasabb, amelyet a narcissin \u00e9s az izovitexin k\u00f6vetett, \u00e9s az \u00e1ltal\u00e1nos \u00e1tviteli sebess\u00e9g viszonylag stabil volt. Az ujjlenyomat-spektrum kutat\u00e1si eredm\u00e9nyek azt mutatt\u00e1k, hogy a Bu Zha Ye f\u0151zetdarabok \u00e9s a standard f\u0151zet k\u00f6z\u00f6tt bizonyos v\u00e1ltoz\u00e1s volt a cs\u00facsok ar\u00e1ny\u00e1ban. Az OPLS-DA elemz\u00e9s \u00f6t szignifik\u00e1nsan elt\u00e9r\u0151 kromatogr\u00e1fi\u00e1s cs\u00facsot azonos\u00edtott, nevezetesen az 1., 2., 3., 4. \u00e9s 6. cs\u00facsot. A 3. cs\u00facs kiv\u00e9tel\u00e9vel, amelyet nem azonos\u00edtottak, az \u00f6sszes t\u00f6bbi kromatogr\u00e1fi\u00e1s cs\u00facs ismert volt. Ezek k\u00f6z\u00fcl az 1., 2. \u00e9s 4. cs\u00facsok fenolos savkomponensek voltak. Ez\u00e9rt a 3. cs\u00facsr\u00f3l felt\u00e9telezhet\u0151, hogy fenolsav. A fenolsavkomponensek k\u00fcl\u00f6nleges jelenl\u00e9tet mutatnak a n\u00f6v\u00e9nyekben, amelyek m\u00e1s vegy\u00fcletek szint\u00e9zis\u00e9nek prekurzorak\u00e9nt szolg\u00e1lhatnak, \u00e9s k\u00fcl\u00f6nb\u00f6z\u0151 vegy\u00fcletekb\u0151l hidroliz\u00e1lhat\u00f3k is. Ez\u00e9rt a Bu Zha Ye standard f\u0151zet\u00e9ben a fenolsav komponensek relat\u00edv cs\u00facster\u00fclet\u00e9nek jelent\u0151s n\u00f6veked\u00e9se a f\u0151z\u00e9si folyamat sor\u00e1n m\u00e1s komponensek hidrol\u00edzis\u00e9nek tulajdon\u00edthat\u00f3. A 6. cs\u00facs izovitexin szerkezete hasonl\u00f3 az S cs\u00facs izovitexin\u00e9hez, polarit\u00e1sban alig k\u00fcl\u00f6nb\u00f6zik, de az \u00e1tad\u00e1si sebess\u00e9gben jelent\u0151s k\u00fcl\u00f6nbs\u00e9g van. \u00dagy tekinthet\u0151, hogy az izovitexin \u00e9s az izovitexin k\u00f6z\u00f6tt bizonyos fok\u00fa interkonverzi\u00f3 zajlik a standard f\u0151zet dekokci\u00f3s folyamata sor\u00e1n, \u00e9s tov\u00e1bbi kutat\u00e1sokra van sz\u00fcks\u00e9g.
\nEz a tanulm\u00e1ny a Bu Zha Ye standard f\u0151zet\u00e9nek kulcsfontoss\u00e1g\u00fa min\u0151s\u00e9gi mutat\u00f3in alapul, \u00e9s m\u00e9lyrehat\u00f3 kutat\u00e1st \u00e9s elemz\u00e9st v\u00e9gez a Bu Zha Ye f\u0151zet standard f\u0151zet\u00e9nek \u00e9rt\u00e9k\u00e1tvitel\u00e9r\u0151l, fontos hivatkoz\u00e1sokat ny\u00fajtva a Bu Zha Ye granul\u00e1tumokra \u00e9s a kapcsol\u00f3d\u00f3 k\u00e9sz\u00edtm\u00e9nyekre vonatkoz\u00f3 min\u0151s\u00e9gi szabv\u00e1nyok megfogalmaz\u00e1s\u00e1hoz.<\/p>","protected":false},"excerpt":{"rendered":"

Study on the transfer of dosage values of Bu Zha Ye decoction pieces and standard decoctions based on UPLC fingerprint spectrum and multi-component quantification Bu Zha Ye is a characteristic medicinal herb in Lingnan, mainly composed of flavonoids, alkaloids, terpenes, organic acids, etc. It has the effects of promoting gastrointestinal peristalsis and gastric juice secretion, […]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"\nStudy on the transfer of dosage values of Bu Zha Ye decoction pieces and standard decoctions based on UPLC fingerprint spectrum and multi-component quantification - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/hu\/dosage-values-of-bu-zha-ye\/\" \/>\n<meta property=\"og:locale\" content=\"hu_HU\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Study on the transfer of dosage values of Bu Zha Ye decoction pieces and standard decoctions based on UPLC fingerprint spectrum and multi-component quantification - 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