{"id":5636,"date":"2024-08-04T20:17:48","date_gmt":"2024-08-04T20:17:48","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=5636"},"modified":"2024-08-04T20:17:48","modified_gmt":"2024-08-04T20:17:48","slug":"hibiscus-mutabilis","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/hu\/hibiscus-mutabilis\/","title":{"rendered":"Az etil-acet\u00e1t antioxid\u00e1ns \u00e9s gyullad\u00e1scs\u00f6kkent\u0151 hat\u00e1s\u00e1nak vizsg\u00e1lata hibiszkuszlevelekben \u00e9s UPLC-Q-Orbitrap HRMS anal\u00edzis"},"content":{"rendered":"

Az etil-acet\u00e1t antioxid\u00e1ns \u00e9s gyullad\u00e1scs\u00f6kkent\u0151 hat\u00e1s\u00e1nak vizsg\u00e1lata hibiszkuszlevelekben \u00e9s UPLC-Q-Orbitrap HRMS anal\u00edzis
\nA Hibiscus mutabilis levelek a Malvaceae csal\u00e1dba tartoz\u00f3 Hibiscus mutabilis L. n\u00f6v\u00e9ny sz\u00e1r\u00edtott levelei. A k\u00ednai gy\u00f3gyszerk\u00f6nyv 2020-as kiad\u00e1s\u00e1ban szerepel, \u00e9s f\u0151k\u00e9nt Szecsu\u00e1nban, Hunanban, Guangdongban, Yunnanban \u00e9s K\u00edna m\u00e1s r\u00e9gi\u00f3iban termesztik. A Qi enyhe, \u00edze cs\u00edp\u0151s \u00e9s lapos, hat\u00e1sa a v\u00e9r h\u0171t\u00e9se, m\u00e9regtelen\u00edt\u00e9s, duzzanatcs\u00f6kkent\u00e9s \u00e9s f\u00e1jdalomcsillap\u00edt\u00e1s. A \"Shennong Bencao Jing\" feljegyz\u00e9sek: \"A k\u00e9s\u0151 ny\u00e1ri \u00e9s kora \u0151szi qi-vel cs\u00edp\u0151s \u00edz\u0171, \u00e9s a Jin Hua kateg\u00f3ri\u00e1j\u00e1ba tartozik, amely k\u00e9pes megtiszt\u00edtani a t\u00fcd\u0151t. A qi nyugodt \u00e9s h\u0171v\u00f6s, ami k\u00e9pes leh\u0171teni a v\u00e9rt, elvezetni a h\u0151t, m\u00e9regtelen\u00edteni, \u00e9s kezelni mindenf\u00e9le karbunkulust, duzzanatot, m\u00e9rget \u00e9s fek\u00e9lyt, valamint enyh\u00edteni a gennyet \u00e9s a f\u00e1jdalmat, \u00e9s a gyermekek alult\u00e1pl\u00e1lts\u00e1g\u00e1t.\". A modern farmakol\u00f3giai kutat\u00e1sok kimutatt\u00e1k, hogy a Mufu Rong Ye j\u00f3 antibakteri\u00e1lis, fert\u0151z\u00e9sg\u00e1tl\u00f3 \u00e9s v\u00edrusellenes hat\u00e1ssal rendelkezik, valamint v\u00e9d\u0151hat\u00e1ssal b\u00edr a vese iszk\u00e9mi\u00e1s-reperf\u00fazi\u00f3s s\u00e9r\u00fcl\u00e9se \u00e9s a kr\u00f3nikus m\u00e1jk\u00e1rosod\u00e1s eset\u00e9n. A hibiszkuszlevelek f\u0151 k\u00e9miai \u00f6sszetev\u0151i k\u00f6z\u00e9 tartoznak a flavonoidok, szerves savak, fenolok, aminosavak, tanninok, cukrok, szteroidok \u00e9s ill\u00f3olajok.
\nA kutat\u00f3csoport el\u0151zetesen meg\u00e1llap\u00edtotta, hogy a hibiszkuszlevelek etil-acet\u00e1t frakci\u00f3ja j\u00f3 antioxid\u00e1ns aktivit\u00e1ssal rendelkezik a DPPH \u00e9s az ABTS szabadgy\u00f6k\u00f6kkel szemben m\u00e9rt kiold\u00e1si ar\u00e1nyok alapj\u00e1n. A hibiszkuszlevelek egyik farmakol\u00f3giai hat\u00f3anyaga a flavonoidok lehetnek. Az in vitro k\u00e9miai m\u00f3dszerek azonban nem k\u00e9pesek pontosan t\u00fckr\u00f6zni a hat\u00f3anyagok val\u00f3di szerep\u00e9t a szervezetekben. Ez\u00e9rt ebben a tanulm\u00e1nyban H2O2 \u00e1ltal induk\u00e1lt oxidat\u00edv stresszk\u00e1rosod\u00e1si modellt \u00e9p\u00edtett\u00fcnk HaCaT sejtekben, m\u00e9rt\u00fck a reakt\u00edv oxig\u00e9nfajok (ROS) termel\u0151d\u00e9s\u00e9t, felt\u00e1rtuk a hibiszkusz levelek k\u00fcl\u00f6nb\u00f6z\u0151 pol\u00e1ris r\u00e9szeinek v\u00e9d\u0151 hat\u00e1s\u00e1t az oxidat\u00edv stresszk\u00e1rosod\u00e1sra HaCaT sejtekben, LPS \u00e1ltal induk\u00e1lt RAW 264.7 sejt gyullad\u00e1sos modellt hoztunk l\u00e9tre, m\u00e9rt\u00fck a sejtek gyullad\u00e1sos faktor expresszi\u00f3j\u00e1nak g\u00e1tl\u00e1s\u00e1nak m\u00e9rt\u00e9k\u00e9t az etil-acet\u00e1t r\u00e9sz \u00e1ltal, \u00e9s tov\u00e1bb vizsg\u00e1ltuk az antioxid\u00e1ns \u00e9s gyullad\u00e1scs\u00f6kkent\u0151 hat\u00e1s\u00e1t sejtszinten. Az UPLC-Q-Orbitrap HRMS technol\u00f3gia alapj\u00e1n azonos\u00edtott\u00e1k a Hibiszkusz levelek etil-acet\u00e1t r\u00e9sz\u00e9nek k\u00e9miai \u00f6sszet\u00e9tel\u00e9t, \u00e9s az akt\u00edv r\u00e9szek pontos elemz\u00e9se kimutatta, hogy az etil-acet\u00e1t r\u00e9sz f\u0151 \u00f6sszetev\u0151i flavonoidok \u00e9s glikozidjaik voltak. E tanulm\u00e1ny c\u00e9lja, hogy \u00e1tfog\u00f3an megmagyar\u00e1zza a Hibiszkusz levelek farmakol\u00f3giai anyagalapj\u00e1t. Tudom\u00e1nyos alapot ny\u00fajt a fejleszt\u00e9s\u00e9hez \u00e9s felhaszn\u00e1l\u00e1s\u00e1hoz.<\/p>\n

\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"<\/p>\n

A ROS a sejtek oxidat\u00edv anyagcser\u00e9j\u00e9nek term\u00e9ke, \u00e9s t\u00falzott n\u00f6veked\u00e9se vagy tart\u00f3s jelenl\u00e9te sejtoxidat\u00edv k\u00e1rosod\u00e1st okozhat. A H2O2 k\u00e9pes a t\u00falzott ROS-termel\u00e9s induk\u00e1l\u00e1s\u00e1ra, \u00edgy ide\u00e1lis induktor az oxidat\u00edv stressz okozta k\u00e1rosod\u00e1si modellekben. A HaCaT sejtek k\u00f6nnyen beszerezhet\u0151k, alkalmazkod\u00f3k\u00e9pesek \u00e9s stabilak. A H2O2 haszn\u00e1lata HaCaT sejtek oxidat\u00edv stressz okozta k\u00e1rosod\u00e1si modellek induk\u00e1l\u00e1s\u00e1ra az antioxid\u00e1ns aktivit\u00e1s \u00e9rt\u00e9kel\u00e9s\u00e9nek gyakori sejtmodellje. A TNF - \u03b1, az IL-6, az iNOS \u00e9s a COX-2 tipikus gyullad\u00e1sos faktorok, az LPS egy gyakori gyullad\u00e1skelt\u0151 faktor, a makrof\u00e1gok pedig a gyullad\u00e1sos v\u00e1lasz fontos r\u00e9sztvev\u0151i, amelyek a gyullad\u00e1s sor\u00e1n k\u00fcl\u00f6nb\u00f6z\u0151 immunpatol\u00f3giai folyamatok kialakul\u00e1s\u00e1t k\u00f6zvet\u00edtik. A RAW 264.7 sejtek LPS indukci\u00f3ja a gyullad\u00e1sos faktorok tanulm\u00e1nyoz\u00e1s\u00e1nak klasszikus sejtmodellje. Jelenleg ezt a k\u00e9t m\u00f3dszert a kozmetikumok r\u00e1nctalan\u00edt\u00f3 \u00e9s nyugtat\u00f3 hat\u00e1sainak megnevez\u00e9s\u00e9t al\u00e1t\u00e1maszt\u00f3 bizony\u00edt\u00e9kk\u00e9nt ismerik el a k\u00f6zz\u00e9tett csoportnorm\u00e1kban. Ez a tanulm\u00e1ny HaCaT sejtek oxidat\u00edv k\u00e1rosod\u00e1si modellj\u00e9t hozta l\u00e9tre, \u00e9s meg\u00e1llap\u00edtotta, hogy a hibiszkuszlevelek k\u00fcl\u00f6nb\u00f6z\u0151 pol\u00e1ris r\u00e9szeinek etil-acet\u00e1t frakci\u00f3ja er\u0151s antioxid\u00e1ns aktivit\u00e1ssal rendelkezik. A 25-200 \u03bc g\/ml koncentr\u00e1ci\u00f3tartom\u00e1nyban a hibiszkuszlevelek etil-acet\u00e1t frakci\u00f3ja jelent\u0151sen cs\u00f6kkentette a ROS (P<0,001) tartalm\u00e1t, enyh\u00edtette az oxidat\u00edv stresszt, \u00e9s j\u00f3 v\u00e9d\u0151hat\u00e1st gyakorolt a sejtek oxidat\u00edv k\u00e1rosod\u00e1si modellj\u00e9re; A Hibiscus syringae levelek etil-acet\u00e1t kivonat\u00e1nak gyullad\u00e1scs\u00f6kkent\u0151 hat\u00e1s\u00e1nak tov\u00e1bbi felt\u00e1r\u00e1s\u00e1t v\u00e9gezt\u00fck, \u00e9s RAW264.7 sejt gyullad\u00e1sos modellt \u00e9p\u00edtett\u00fcnk. Amikor az etil-acet\u00e1t kivonat koncentr\u00e1ci\u00f3ja 200 \u03bc g\/ml volt, jelent\u0151sen g\u00e1tolta a TNF - \u03b1, IL-6, iNOS \u00e9s COX-2 mRNS expresszi\u00f3j\u00e1t a RAW 264.7 sejtekben (P<0,001), ami j\u00f3 gyullad\u00e1scs\u00f6kkent\u0151 aktivit\u00e1st mutat. Ez tov\u00e1bb tiszt\u00e1zta a Hibiscus syringae levelek antioxid\u00e1ns \u00e9s gyullad\u00e1scs\u00f6kkent\u0151 akt\u00edv helyeit, elm\u00e9leti alapot biztos\u00edtva a farmakol\u00f3giai aktivit\u00e1s tov\u00e1bbi kutat\u00e1s\u00e1hoz \u00e9s a kozmetikai alkalmaz\u00e1sok fejleszt\u00e9s\u00e9hez.<\/p>\n

Az ultra-nagy teljes\u00edtm\u00e9ny\u0171 folyad\u00e9kkromatogr\u00e1fi\u00e1s kvadrupolos elektrosztatikus mez\u0151 orbit\u00e1lis csapda nagyfelbont\u00e1s\u00fa t\u00f6megspektrometri\u00e1s technol\u00f3gia alkalmas a hagyom\u00e1nyos k\u00ednai gy\u00f3gy\u00e1szatban tal\u00e1lhat\u00f3 \u00f6sszetett t\u00f6bbkomponens\u0171 rendszerek elemz\u00e9s\u00e9re, \u00e9s jellemz\u0151i a nagy felbont\u00e1s, a nagy min\u0151s\u00e9gi pontoss\u00e1g \u00e9s a nagy detekt\u00e1l\u00e1si hat\u00e9konys\u00e1g. Ebben a tanulm\u00e1nyban el\u0151sz\u00f6r alkalmazt\u00e1k az UPLC-Q-Orbitrap HRMS technol\u00f3gi\u00e1t a hibiszkuszlevelek etil-acet\u00e1t hat\u00e1sos r\u00e9szeiben l\u00e9v\u0151 komplex komponensek \u00e1tfog\u00f3 min\u0151s\u00e9gi elemz\u00e9s\u00e9re. 28 k\u00e9miai komponenst azonos\u00edtottak, k\u00f6zt\u00fck 10 flavonoidot, f\u0151k\u00e9nt rutint, naringint, izoquercitrint stb.; 9 szerves savkomponens van, f\u0151k\u00e9nt ferulasav, k\u00e1v\u00e9sav, szalicilsav stb.; Vannak tov\u00e1bb\u00e1 kumarinok, fenolok, nukleozidok, egyenk\u00e9nt 2 \u00e9s 3 egy\u00e9b komponens, amelyek \u00f6sszhangban vannak azokkal a jelent\u00e9sekkel, amelyek szerint a hibiszkuszlevelek gazdagok flavonoidokban \u00e9s fenolsavakban. A flavonoidok fenolos hidrog\u00e9nmolekul\u00e1kat tartalmaznak, amelyek hidrog\u00e9ndonork\u00e9nt m\u0171k\u00f6dhetnek, \u00edgy er\u0151s antioxid\u00e1nsok, kiv\u00e1l\u00f3 gyullad\u00e1scs\u00f6kkent\u0151 hat\u00e1ssal. A rutin n\u00f6velheti a katal\u00e1z (CAT) \u00e9s a szuperoxid-dizmut\u00e1z (SOD) aktivit\u00e1s\u00e1t Parkinson-k\u00f3r (PD) modellekben; az ez\u00fcsth\u00e1rs g\u00e1tolhatja a prosztaglandin E2 (PGE2) termel\u00e9s\u00e9t \u00e9s az NF - \u03ba B \u00e9s MAPK jel\u00e1tviteli utak aktiv\u00e1l\u00f3d\u00e1s\u00e1t; A kvercetin g\u00e1tolhatja a ROS \u00e1ltal k\u00f6zvet\u00edtett m\u00e1jr\u00e1kot az olyan enzimek, mint a SOD \u00e9s a CAT, valamint a nem enzimatikus antioxid\u00e1ns v\u00e9delmi rendszerek, mint a glutation (GSH) felszab\u00e1lyoz\u00e1s\u00e1val; Az izokvercetin n\u00f6velheti a SOD \u00e9s cs\u00f6kkentheti az MDA-szintet, g\u00e1tolhatja a MEK\/ERK jel\u00e1tviteli \u00fatvonalat, \u00e9s elnyomhatja az oxidat\u00edv stresszv\u00e1laszt. Az azelainsav kiv\u00e9tel\u00e9vel az \u00f6sszes szerves savkomponens fenolsav. Sz\u00e1mos tanulm\u00e1ny kimutatta, hogy a fenolsavak er\u0151s reduk\u00e1l\u00f3 tulajdons\u00e1gokkal rendelkeznek a molekulaszerkezet\u00fckben tal\u00e1lhat\u00f3 polifenolos hidroxilcsoportok miatt, \u00edgy a szabadgy\u00f6k-lebont\u00f3 hat\u00e1s\u00fa vegy\u00fcletek tipikus oszt\u00e1ly\u00e1ba tartoznak. P\u00e9ld\u00e1ul a ferulasav fokozhatja az eg\u00e9rsz\u00e9rum antioxid\u00e1ns kapacit\u00e1s\u00e1t, \u00e9s jav\u00edthatja az antibiotikumok b\u00e9lanyagcser\u00e9re \u00e9s gyullad\u00e1sos v\u00e1laszra gyakorolt negat\u00edv hat\u00e1sait egerekben; a szalicilsav az antioxid\u00e1ns enzimaktivit\u00e1s javul\u00e1s\u00e1t id\u00e9zheti el\u0151, \u00e9s enyh\u00edtheti a reakt\u00edv oxig\u00e9nfajok biol\u00f3giai oxid\u00e1ci\u00f3j\u00e1t. Ez a tanulm\u00e1ny meg\u00e1llap\u00edtotta, hogy ez a k\u00e9tf\u00e9le komponens a hibiszkuszlevelek etil-acet\u00e1t r\u00e9sz\u00e9nek f\u0151 k\u00e9miai \u00f6sszetev\u0151je, ami tov\u00e1bb mutatja, hogy a hibiszkuszlevelek antioxid\u00e1ns \u00e9s gyullad\u00e1scs\u00f6kkent\u0151 hat\u00e1s\u00fa farmakol\u00f3giai anyagalapja f\u0151k\u00e9nt flavonoidokb\u00f3l \u00e9s szerves savakb\u00f3l \u00e1ll. Ugyanakkor a tanulm\u00e1ny egy \u00faj, eddig nem ismertetett flavonoidkomponenst is felfedezett a Hibiscus syringae leveleiben, amely a trifoli\u00e1t-gl\u00fckozid, k\u00e9t \u00faj fenolos komponens a 4-nitrofenol \u00e9s a 4-nitrokatechol, egy \u00faj nukleozidkomponens az adenin, \u00e9s k\u00e9t \u00faj aldehidkomponens a 2,5-dihidroxibenzaldehid \u00e9s a vanillin, gazdag\u00edtva a Hibiscus syringae leveleinek k\u00e9miai \u00f6sszet\u00e9teli k\u00f6nyvt\u00e1r\u00e1t.<\/p>\n

\u00d6sszefoglalva, a Hibiscus syringae leveleinek etil-acet\u00e1t kivonata nagym\u00e9rt\u00e9kben enyh\u00edtheti a sejtek oxidat\u00edv k\u00e1rosod\u00e1s\u00e1t, ellen\u00e1llhat az oxidat\u00edv stresszv\u00e1lasznak, \u00e9s g\u00e1tolhatja a gyullad\u00e1sos faktorok kifejez\u0151d\u00e9s\u00e9t. Farmakol\u00f3giai anyagainak alapj\u00e1t a flavonoidok \u00e9s a szerves savak k\u00e9pezhetik, ami elm\u00e9leti alapot biztos\u00edt a Hibiscus syringae leveleinek antioxid\u00e1ns \u00e9s gyullad\u00e1scs\u00f6kkent\u0151 mechanizmusainak tov\u00e1bbi kutat\u00e1s\u00e1hoz, valamint a term\u00e9szetes term\u00e9kek fejleszt\u00e9s\u00e9hez \u00e9s felhaszn\u00e1l\u00e1s\u00e1hoz.<\/p>","protected":false},"excerpt":{"rendered":"

Study on the antioxidant and anti-inflammatory effects of ethyl acetate in hibiscus leaves and UPLC-Q-Orbitrap HRMS analysis Hibiscus mutabilis leaves are dried leaves of Hibiscus mutabilis L., a plant in the Malvaceae family. They are included in the 2020 edition of the Chinese Pharmacopoeia and are mainly produced in Sichuan, Hunan, Guangdong, Yunnan, and other […]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"\nStudy on the antioxidant and anti-inflammatory effects of ethyl acetate in hibiscus leaves and UPLC-Q-Orbitrap HRMS analysis - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/hu\/hibiscus-mutabilis\/\" \/>\n<meta property=\"og:locale\" content=\"hu_HU\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Study on the antioxidant and anti-inflammatory effects of ethyl acetate in hibiscus leaves and UPLC-Q-Orbitrap HRMS analysis - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"Study on the antioxidant and anti-inflammatory effects of ethyl acetate in hibiscus leaves and UPLC-Q-Orbitrap HRMS analysis Hibiscus mutabilis leaves are dried leaves of Hibiscus mutabilis L., a plant in the Malvaceae family. 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