{"id":5512,"date":"2024-08-04T19:31:38","date_gmt":"2024-08-04T19:31:38","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=5512"},"modified":"2024-08-04T19:31:38","modified_gmt":"2024-08-04T19:31:38","slug":"uplc-q-orbitrap-hrms","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/hu\/uplc-q-orbitrap-hrms\/","title":{"rendered":"UPLC-Q-Orbitrap HRMS ujjlenyomat \u00e9s a Jinlongdeng antibakteri\u00e1lis aktivit\u00e1s\u00e1nak spektrum-hat\u00e1s kapcsolata"},"content":{"rendered":"

UPLC-Q-Orbitrap HRMS ujjlenyomat \u00e9s a Jinlongdeng antibakteri\u00e1lis aktivit\u00e1s\u00e1nak spektrum-hat\u00e1s kapcsolata
\nA Jin Deng Deng, m\u00e1s n\u00e9ven Suanjiang vagy Red Maiden, a Solanaceae csal\u00e1dba tartoz\u00f3 Physalis alkekengi L. var. franchetii (Mast.) Makino sz\u00e1r\u00edtott vagy term\u0151 kehelye. Savany\u00fa \u00e9s keser\u0171 \u00edz\u0171, hideg term\u00e9szet\u0171, a t\u00fcd\u0151 \u00e9s a l\u00e9p meridi\u00e1nokhoz tartozik. H\u0151tiszt\u00edt\u00f3 \u00e9s m\u00e9regtelen\u00edt\u0151, a torok m\u0171k\u00f6d\u00e9s\u00e9t el\u0151seg\u00edt\u0151 \u00e9s a v\u00e1lad\u00e9kot felold\u00f3, vizelethajt\u00f3 \u00e9s a nyirokrendszert tiszt\u00edt\u00f3 hat\u00e1s\u00fa. Klinikailag torokf\u00e1j\u00e1s, t\u00fcd\u0151h\u0151 k\u00f6h\u00f6g\u00e9s \u00e9s vizelettart\u00e1si zavarok kezel\u00e9s\u00e9re haszn\u00e1lj\u00e1k. A Jinlongdeng k\u00e9miai \u00f6sszet\u00e9tele v\u00e1ltozatos, els\u0151sorban olyan vegy\u00fcleteket tartalmaz, mint keser\u0171anyagok, flavonoidok, szachar\u00f3z-\u00e9szterek, fenilpropanoidok, alkaloidok stb. A modern farmakol\u00f3giai vizsg\u00e1latok kimutatt\u00e1k, hogy a Jinlongdeng alkoholos kivonata \u00e9s dikl\u00f3rmet\u00e1n kivonata j\u00f3 g\u00e1tl\u00f3 hat\u00e1ssal rendelkezik a Gram-pozit\u00edv bakt\u00e9riumokkal, Gram-negat\u00edv bakt\u00e9riumokkal \u00e9s a Candida albicans-szal szemben. A savany\u00fa keser\u0171anyagok egyes \u00f6sszetev\u0151inek jelent\u0151s antibakteri\u00e1lis hat\u00e1sa is bizony\u00edtott. Jelenleg sz\u00e1mos tanulm\u00e1ny foglalkozik a Jinlongdeng antibakteri\u00e1lis hat\u00e1s\u00e1val mind hazai, mind nemzetk\u00f6zi szinten, de az anyagi alapja m\u00e9g nem teljesen vil\u00e1gos.<\/p>\n

A hagyom\u00e1nyos k\u00ednai orvosl\u00e1s maga is \u00f6sszetett \u00e9s v\u00e1ltozatos \u00f6sszetev\u0151ket tartalmaz, \u00e9s ter\u00e1pi\u00e1s hat\u00e1sainak kifejt\u00e9sekor a t\u00f6bbc\u00e9l\u00fa \u00e9s \u00e1tfog\u00f3 szab\u00e1lyoz\u00e1s jellemz\u0151ivel rendelkezik. A hagyom\u00e1nyos k\u00ednai gy\u00f3gy\u00e1szat spektruma \u00e9s hat\u00e9konys\u00e1ga k\u00f6z\u00f6tti kapcsolat a hagyom\u00e1nyos k\u00ednai gy\u00f3gy\u00e1szat ujjlenyomat-spektrum\u00e1nak tanulm\u00e1nyoz\u00e1s\u00e1n, az ujjlenyomat-spektrum \u00e9s a farmakol\u00f3giai hat\u00e1sok \u00f6sszekapcsol\u00e1s\u00e1n, a spektrum-hat\u00e9konys\u00e1gi kapcsolat kutat\u00e1s\u00e1n, valamint a farmakol\u00f3giai hat\u00e1sok kifejt\u00e9s\u00e9nek anyagi alapj\u00e1nak tov\u00e1bbi tiszt\u00e1z\u00e1s\u00e1n alapul. Jelenleg viszonylag kev\u00e9s kutat\u00e1s van a Jinlongdeng ujjlenyomat spektrum\u00e1nak \u00e9s spektr\u00e1lis hat\u00e9konys\u00e1g\u00e1nak kapcsolat\u00e1r\u00f3l. Ez\u00e9rt e tanulm\u00e1ny c\u00e9lja, hogy felt\u00e1rja a Jinlongdeng antibakteri\u00e1lis aktivit\u00e1s\u00e1nak anyagi alapj\u00e1t a spektrumhat\u00e1s \u00f6sszef\u00fcgg\u00e9s\u00e9b\u0151l. H\u00e1rom k\u00fcl\u00f6nb\u00f6z\u0151 eredet\u0171 Jinlongeng l\u00e1mp\u00e1s 16 t\u00e9tel\u00e9nek t\u00f6megspektrometri\u00e1s ujjlenyomat-spektrum\u00e1nak l\u00e9trehoz\u00e1s\u00e1val \u00e9s a Staphylococcus aureus \u00e9s Escherichia coli elleni antibakteri\u00e1lis aktivit\u00e1suk meghat\u00e1roz\u00e1s\u00e1val sz\u00fcrke korrel\u00e1ci\u00f3s anal\u00edzist alkalmaztunk a jellemz\u0151 cs\u00facsok \u00e9s az antibakteri\u00e1lis aktivit\u00e1suk k\u00f6z\u00f6tti \u00f6sszef\u00fcgg\u00e9s felt\u00e1r\u00e1s\u00e1ra, hogy tiszt\u00e1zzuk az antibakteri\u00e1lis aktivit\u00e1suk anyagi alapj\u00e1t, \u00e9s alapot biztos\u00edtsunk a Jinlongeng l\u00e1mp\u00e1sok tov\u00e1bbi fejleszt\u00e9s\u00e9hez, felhaszn\u00e1l\u00e1s\u00e1hoz \u00e9s min\u0151s\u00e9gellen\u0151rz\u00e9si kutat\u00e1s\u00e1hoz.<\/p>\n

\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"A k\u00eds\u00e9rlet korai szakasz\u00e1ban a k\u00fcl\u00f6nb\u00f6z\u0151 metanol-koncentr\u00e1ci\u00f3k (50%, 70%, 100%) hat\u00e1s\u00e1t vizsg\u00e1lt\u00e1k a Jinlongdeng gy\u00f3gy\u00e1szati anyagok extrakci\u00f3s hat\u00e9konys\u00e1g\u00e1ra, \u00e9s meg\u00e1llap\u00edtott\u00e1k, hogy a cs\u00facs a 70% metanol-v\u00edz extrakci\u00f3 sor\u00e1n volt a legmagasabb. Ek\u00f6zben a t\u00f6megspektrometri\u00e1s k\u00f6r\u00fclm\u00e9nyek vizsg\u00e1lata sor\u00e1n meg\u00e1llap\u00edtott\u00e1k, hogy a negat\u00edv ion m\u00f3dban a teljes ionkromatogram stabilabb volt, \u00e9s a cs\u00facs alakja vonz\u00f3bb volt. Ez\u00e9rt a k\u00eds\u00e9rletet negat\u00edv ionp\u00e1szt\u00e1z\u00f3 \u00fczemm\u00f3dban v\u00e9gezt\u00fck.<\/p>\n

Ez a tanulm\u00e1ny 16 t\u00e9tel Jinlongdeng gy\u00f3gyn\u00f6v\u00e9ny ujjlenyomat spektrum\u00e1t \u00e1ll\u00edtotta \u00f6ssze, 38 k\u00f6z\u00f6s cs\u00facsot extrah\u00e1lt, \u00e9s a hasonl\u00f3s\u00e1g 0,95 felett volt, ami azt jelzi, hogy a k\u00fcl\u00f6nb\u00f6z\u0151 eredet\u0171 Jinlongdeng gy\u00f3gyn\u00f6v\u00e9nyek min\u0151s\u00e9ge viszonylag stabil; mind a klaszterelemz\u00e9s, mind a PCA elemz\u00e9s k\u00e9pes a mint\u00e1kat k\u00fcl\u00f6nb\u00f6z\u0151 eredet szerint csoportos\u00edtani, ami azt jelzi, hogy a gy\u00f3gy\u00e1szati anyagok n\u00f6veked\u00e9si k\u00f6rnyezete a min\u0151s\u00e9gi k\u00fcl\u00f6nbs\u00e9geket okoz\u00f3 f\u0151 t\u00e9nyez\u0151.<\/p>\n

Ugyanakkor ez a tanulm\u00e1ny meghat\u00e1rozta a Jin Deng l\u00e1mp\u00e1s kivonatok 16 t\u00e9tel\u00e9nek antibakteri\u00e1lis aktivit\u00e1s\u00e1t Staphylococcus aureus \u00e9s Escherichia coli ellen. Els\u0151 alkalommal kombin\u00e1lt\u00e1k az ujjlenyomat-spektrumokat a farmakol\u00f3giai eredm\u00e9nyekkel, \u00e9s sz\u00fcrke korrel\u00e1ci\u00f3s anal\u00edzist alkalmaztak a Jin Deng l\u00e1mp\u00e1s spektr\u00e1lis hat\u00e9konys\u00e1gi kapcsolat\u00e1nak tanulm\u00e1nyoz\u00e1s\u00e1ra. Az eredm\u00e9nyek azt mutatt\u00e1k, hogy a Jinlongdeng antibakteri\u00e1lis aktivit\u00e1sa t\u00f6bb komponens egy\u00fcttes hat\u00e1s\u00e1nak eredm\u00e9nye lehet. K\u00f6z\u00fcl\u00fck a jellegzetes cs\u00facsok, amelyek jelent\u0151sen hozz\u00e1j\u00e1rulnak az antibakteri\u00e1lis aktivit\u00e1shoz (korrel\u00e1ci\u00f3 \u2265 0,85), mind szachar\u00f3z-zs\u00edrsav-\u00e9szterekb\u0151l \u00e1llnak, ami azt jelzi, hogy ezek a komponensek jelent\u0151sen hozz\u00e1j\u00e1rulnak a Jinlongdeng antibakteri\u00e1lis aktivit\u00e1s\u00e1hoz.<\/p>\n

A m\u00faltban a tud\u00f3sok Jinlongdenggel kapcsolatos kutat\u00e1sai els\u0151sorban a savas keser\u0171 vegy\u00fcletek \u00f6sszetett k\u00e9miai szerkezet\u00e9re \u00e9s jelent\u0151s biol\u00f3giai aktivit\u00e1s\u00e1ra \u00f6sszpontos\u00edtottak, mik\u00f6zben elhanyagolt\u00e1k a szachar\u00f3z zs\u00edrsav\u00e9szterek tanulm\u00e1nyoz\u00e1s\u00e1t. A szachar\u00f3z zs\u00edrsav\u00e9szter komponensek szerkezeti jellemz\u0151i az, hogy a szachar\u00f3z szolg\u00e1l az anyamagk\u00e9nt, \u00e9s egy vagy t\u00f6bb zs\u00edrsavl\u00e1nc-t\u00f6red\u00e9khez kapcsol\u00f3dik \u00e9szterk\u00f6t\u00e9seken kereszt\u00fcl. A modern farmakol\u00f3giai kutat\u00e1sok kimutatt\u00e1k, hogy az ilyen t\u00edpus\u00fa vegy\u00fcletek jelent\u0151s antibakteri\u00e1lis, gyullad\u00e1scs\u00f6kkent\u0151, hipoglik\u00e9mi\u00e1s, daganatellenes, rovar\u00f6l\u0151 \u00e9s egy\u00e9b tev\u00e9kenys\u00e9ggel rendelkeznek. Ugyanakkor a szachar\u00f3z-zs\u00edrsav\u00e9szterek j\u00f3 biol\u00f3giai lebonthat\u00f3s\u00e1ggal \u00e9s toxikol\u00f3giai tulajdons\u00e1gokkal rendelkeznek, \u00edgy alkalmaz\u00e1si lehet\u0151s\u00e9geik sz\u00e9lesk\u00f6r\u0171ek \u00e9s figyelmet \u00e9rdemelnek.<\/p>\n

\u00d6sszefoglalva, ez a tanulm\u00e1ny sikeresen meghat\u00e1rozta a Lonicera japonica kivonat\u00e1nak ujjlenyomat-spektrum\u00e1t, \u00e9s els\u0151 alkalommal tanulm\u00e1nyozta annak spektr\u00e1lis hat\u00e1s-\u00f6sszet\u00e9tel\u00e9t. Az eredm\u00e9nyek azt mutatt\u00e1k, hogy a szachar\u00f3z-zs\u00edrsav\u00e9szter komponensek nagym\u00e9rt\u00e9kben korrel\u00e1ltak a Lonicera japonica antibakteri\u00e1lis aktivit\u00e1s\u00e1val. Egyedi szerkezete miatt azonban ebben a k\u00eds\u00e9rletben nem volt lehets\u00e9ges a szerkezet\u00e9nek m\u00e9lyrehat\u00f3 elemz\u00e9se t\u00f6megspektrometri\u00e1val. A j\u00f6v\u0151ben a Jin Dengben tal\u00e1lhat\u00f3 ilyen t\u00edpus\u00fa komponenst izol\u00e1lni \u00e9s tiszt\u00edtani fogj\u00e1k, hogy ellen\u0151rizz\u00e9k antibakteri\u00e1lis aktivit\u00e1s\u00e1t. Ennek alapj\u00e1n a szerkezet-aktivit\u00e1s \u00f6sszef\u00fcgg\u00e9st \u00f6sszefoglaljuk, hogy referenci\u00e1t ny\u00fajtsunk a Jin Deng antibakteri\u00e1lis aktivit\u00e1s\u00e1nak \u00e9s hat\u00e1smechanizmus\u00e1nak tov\u00e1bbi kutat\u00e1s\u00e1hoz.<\/p>","protected":false},"excerpt":{"rendered":"

UPLC-Q-Orbitrap HRMS fingerprint and spectrum effect relationship of antibacterial activity of Jinlongdeng Jin Deng Deng, also known as Suanjiang or Red Maiden, is the dried or fruiting calyx of Physalis alkekengi L. var. franchetii (Mast.) Makino, a plant in the Solanaceae family. Sour and bitter in taste, cold in nature, belongs to the lung and […]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"\nUPLC-Q-Orbitrap HRMS fingerprint and spectrum effect relationship of antibacterial activity of Jinlongdeng - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/hu\/uplc-q-orbitrap-hrms\/\" \/>\n<meta property=\"og:locale\" content=\"hu_HU\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"UPLC-Q-Orbitrap HRMS fingerprint and spectrum effect relationship of antibacterial activity of Jinlongdeng - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"UPLC-Q-Orbitrap HRMS fingerprint and spectrum effect relationship of antibacterial activity of Jinlongdeng Jin Deng Deng, also known as Suanjiang or Red Maiden, is the dried or fruiting calyx of Physalis alkekengi L. var. franchetii (Mast.) Makino, a plant in the Solanaceae family. 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Makino, a plant in the Solanaceae family. 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