{"id":5358,"date":"2024-08-04T18:33:21","date_gmt":"2024-08-04T18:33:21","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=5358"},"modified":"2024-08-04T18:33:21","modified_gmt":"2024-08-04T18:33:21","slug":"gc-ms","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/hu\/gc-ms\/","title":{"rendered":"A Shanyinhua olaj teljes komponens jellemz\u00e9se \u00e9s in vitro gyullad\u00e1scs\u00f6kkent\u0151 \u00e9s antioxid\u00e1ns aktivit\u00e1sa GC-MS \u00e9s LC-MS seg\u00edts\u00e9g\u00e9vel"},"content":{"rendered":"

A Shanyinhua olaj teljes komponens jellemz\u00e9se \u00e9s in vitro gyullad\u00e1scs\u00f6kkent\u0151 \u00e9s antioxid\u00e1ns aktivit\u00e1sa GC-MS \u00e9s LC-MS seg\u00edts\u00e9g\u00e9vel
\nA hegyi ez\u00fcstvir\u00e1g a Lonicera csal\u00e1dba tartozik, a Lonicera macranthoides Hand- Mazz.\u3001 Lonicera hypoglauca Miq., Lonicera hypoglauca Miq., Lonicera hypoglauca Miq., Lonicera hypoglauca Miq., Lonicera hypoglauca Miq., Lonicera hypoglauca Miq., Lonicera hypoglauca Miq., Lonicera hypoglauca Miq. A Lonicera confusa DC vagy Lonicera fulvotontosa Hsu et S. sz\u00e1r\u00edtott vir\u00e1gbimb\u00f3i vagy vir\u00e1gai a frissen ny\u00edl\u00f3 vir\u00e1gokkal. C. Cheng D\u00e9l-K\u00edn\u00e1ban. A Shanyinhua-t els\u0151sorban a bakt\u00e9riumok \u00e9s v\u00edrusok \u00e1ltal kiv\u00e1ltott korai fert\u0151z\u00e9sek, sebek, t\u00e1lyogok, kel\u00e9sek \u00e9s duzzanatok kezel\u00e9s\u00e9re haszn\u00e1lj\u00e1k, amelyeket els\u0151sorban a k\u00fcls\u0151 sz\u00e9lh\u0151 vagy a j\u00e1rv\u00e1nyos l\u00e1z okozta h\u0151, b\u0151rp\u00edr, f\u00e1jdalom \u00e9s duzzanat jellemez. A lonc a hagyom\u00e1nyos k\u00ednai szabadalmi gy\u00f3gyszerek \u00e9s egyszer\u0171 k\u00e9sz\u00edtm\u00e9nyek f\u0151 \u00f6sszetev\u0151je C-vitamin Yinqiao tabletta, hideg k\u00f6h\u00f6g\u00e9s elleni szirup \u00e9s \u00f6sszetett Daqingye kever\u00e9k. Ezenk\u00edv\u00fcl a hegyi ez\u00fcstvir\u00e1got gyakran haszn\u00e1lj\u00e1k nyersanyagk\u00e9nt k\u00fcl\u00f6nb\u00f6z\u0151 eg\u00e9szs\u00e9g\u00fcgyi term\u00e9kek, p\u00e9ld\u00e1ul tea \u00e9s bor el\u0151\u00e1ll\u00edt\u00e1s\u00e1hoz.
\nSz\u00e1mos n\u00f6v\u00e9nyk\u00e9miai tanulm\u00e1ny kimutatta, hogy a Shanyinhua flavonoidokat, szerves savakat, iridoidokat, szaponinokat \u00e9s olajat tartalmaz. Az olaj k\u00e9miai \u00f6sszet\u00e9tel\u00e9nek \u00e9s biol\u00f3giai aktivit\u00e1s\u00e1nak m\u00e9lyrehat\u00f3 vizsg\u00e1lat\u00e1r\u00f3l azonban csak korl\u00e1tozott irodalom \u00e1ll rendelkez\u00e9sre. A kutat\u00e1si m\u00f3dszerek szempontj\u00e1b\u00f3l a k\u00f6z\u00f6lt irodalom f\u0151k\u00e9nt g\u00e1zkromatogr\u00e1fia-t\u00f6megspektrometri\u00e1s (GC-MS) \u00e9s g\u00e1zkromatogr\u00e1fia-t\u00f6megspektrometri\u00e1val kombin\u00e1lt szil\u00e1rd f\u00e1zis\u00fa fejt\u00e9r extrakci\u00f3t (HS-SPME-GC-MS) alkalmaz. Az extrakci\u00f3s m\u00f3dszerekb\u0151l meg\u00e1llap\u00edthat\u00f3, hogy els\u0151sorban ultrahangot, mikrohull\u00e1m\u00fa, Soxhlet extrakci\u00f3t \u00e9s g\u0151zdesztill\u00e1ci\u00f3t alkalmaznak. Az extrakci\u00f3s m\u00f3dszerek sz\u00e9les sk\u00e1l\u00e1j\u00e1t tekintve viszonylag kev\u00e9s kutat\u00e1s foglalkozik a CO2 szuperkritikus extrakci\u00f3val az olaj kinyer\u00e9s\u00e9re. A kimutat\u00e1si m\u00f3dszerek teljess\u00e9ge szempontj\u00e1b\u00f3l hi\u00e1nyoznak a k\u00f6zepes polarit\u00e1s\u00fa anyagok kimutat\u00e1sa az olajban. Ezenk\u00edv\u00fcl a Shanyinhua olaj j\u00f3 biol\u00f3giai aktivit\u00e1ssal rendelkezik, \u00e9s \u00e9rdemes tov\u00e1bbi fejleszt\u00e9sre \u00e9s kutat\u00e1sra.
\nEz\u00e9rt ez a tanulm\u00e1ny a hegyi ez\u00fcstvir\u00e1g olajat tekinti kutat\u00e1si t\u00e1rgynak, \u00e9s CO2 szuperkritikus extrakci\u00f3t alkalmaz a hegyi ez\u00fcstvir\u00e1g olaj kinyer\u00e9s\u00e9re; az olajban l\u00e9v\u0151 ill\u00e9kony \u00e9s kis pol\u00e1ris komponensek elemz\u00e9se a metil\u00e1l\u00e1s \u00e9s nem metil\u00e1l\u00e1s kombin\u00e1l\u00e1s\u00e1val GC-MS-szel; a k\u00f6zepes polarit\u00e1s\u00fa komponensek azonos\u00edt\u00e1sa az olajban ultra-nagy teljes\u00edtm\u00e9ny\u0171 folyad\u00e9kkromatogr\u00e1fia-t\u00f6megspektrometri\u00e1val (UHPLC-QE-MS) egy standard spektrumk\u00f6nyvt\u00e1rral kombin\u00e1lva. A Shanyinhua k\u00e9miai \u00f6sszet\u00e9tel\u00e9nek \u00e1tfog\u00f3 elemz\u00e9se \u00e9rdek\u00e9ben. Ugyanakkor ez a tanulm\u00e1ny in vitro antioxid\u00e1ns m\u00f3dszereket \u00e9s in vitro sejtgyullad\u00e1scs\u00f6kkent\u0151 k\u00eds\u00e9rleteket alkalmazott a Lonicera japonica olaj antioxid\u00e1ns \u00e9s gyullad\u00e1scs\u00f6kkent\u0151 hat\u00e1sainak \u00e9rt\u00e9kel\u00e9s\u00e9re, tudom\u00e1nyos \u00fatmutat\u00e1st ny\u00fajtva annak tov\u00e1bbi fejleszt\u00e9s\u00e9hez \u00e9s felhaszn\u00e1l\u00e1s\u00e1hoz.<\/p>\n

\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"
\nEbben a tanulm\u00e1nyban a Lonicera japonica olaj in vitro gyullad\u00e1scs\u00f6kkent\u0151 \u00e9s antioxid\u00e1ns hat\u00e1s\u00e1t vizsg\u00e1lt\u00e1k egy lipopoliszacharid (LPS) \u00e1ltal kiv\u00e1ltott sejtgyullad\u00e1sos modell seg\u00edts\u00e9g\u00e9vel, \u00e9s elv\u00e9gezt\u00e9k a Lonicera japonica olaj teljes komponensanal\u00edzis\u00e9t GC-MS \u00e9s UHPLC-QE-MS alapj\u00e1n. A Zhongshan Silver Flower Oil extrakci\u00f3ja ebben a cikkben a szuperkritikus CO2 extrakci\u00f3s m\u00f3dszert alkalmazza, amely j\u00f3 old\u00f3szeroldhat\u00f3s\u00e1ggal, alacsony extrakci\u00f3s h\u0151m\u00e9rs\u00e9klettel \u00e9s nagy biztons\u00e1ggal rendelkezik, \u00e9s hat\u00e9konyan megakad\u00e1lyozza a gy\u00f3gy\u00e1szati anyagok hat\u00f3anyagainak oxidat\u00edv boml\u00e1s\u00e1t. A GC-MS elemz\u00e9ssel \u00f6sszesen 66 vegy\u00fcletet azonos\u00edtottak a Shanyinhua olajban, k\u00f6zt\u00fck f\u0151k\u00e9nt zs\u00edrsavakat (metil-palmit\u00e1t, metil-linole\u00e1t stb.), szterolokat (\u03b3-szitoszterin, stigmaszterin stb.) \u00e9s alk\u00e1nokat (szkval\u00e1n, n-tetradecan stb.). Kutat\u00e1sok kimutatt\u00e1k, hogy a t\u00f6bbsz\u00f6r\u00f6sen tel\u00edtetlen zs\u00edrsavak, mint p\u00e9ld\u00e1ul a linolsav, k\u00e9pesek g\u00e1tolni a gyullad\u00e1st okoz\u00f3 medi\u00e1torok \u00e9s citokinek gyullad\u00e1skelt\u0151 hat\u00e1s\u00e1t, \u00e9s enyh\u00edteni az akut \u00e9s kr\u00f3nikus gyullad\u00e1ssal \u00f6sszef\u00fcgg\u0151 betegs\u00e9geket; a szterolvegy\u00fcletek, mint p\u00e9ld\u00e1ul a stigmasterol hat\u00e9konyan k\u00e9sleltetik a lipidperoxid\u00e1ci\u00f3s reakci\u00f3kat, g\u00e1tolj\u00e1k az olajsav\u00e9rt\u00e9k \u00e9s a peroxid\u00e9rt\u00e9k n\u00f6veked\u00e9s\u00e9t, \u00e9s jelent\u0151sen jobb antioxid\u00e1ns hat\u00e1ssal rendelkeznek, mint a dibutil-hidroxitoluol (BHT) \u00e9s a C-vitamin. Az UHPLC-QE-MS seg\u00edts\u00e9g\u00e9vel 142, illetve 83 vegy\u00fcletet azonos\u00edtottak pozit\u00edv, illetve negat\u00edv ionm\u00f3dban, amelyek k\u00f6z\u00f6tt f\u0151k\u00e9nt terp\u00e9nek, kumarinok \u00e9s sz\u00e1rmaz\u00e9kaik, fenilpropanoidok, alkaloidok, flavonoidok \u00e9s egy\u00e9b vegy\u00fcletek voltak. Az irodalmi jelent\u00e9sek szerint a terpenoidok, mint p\u00e9ld\u00e1ul a D2-vitamin \u00e9s az ursolsav, in vivo \u00e9s in vitro cs\u00f6kkenthetik az IL-6 \u00e9s TNF - \u03b1 expresszi\u00f3j\u00e1t, gyullad\u00e1scs\u00f6kkent\u0151 hat\u00e1st fejtenek ki, \u00e9s jelent\u0151s in vitro antioxid\u00e1ns kapacit\u00e1ssal rendelkeznek. Az olyan flavonoidok, mint a naringin \u00e9s a brazil hematoxin g\u00e1tolhatj\u00e1k az LPS \u00e1ltal induk\u00e1lt gyullad\u00e1sos faktorok, mint az NO, a PGE2 \u00e9s a TNF - \u03b1 felszabadul\u00e1s\u00e1t RAW 264.7 sejtekben, \u00e9s farmakol\u00f3giai hat\u00e1sokat fejthetnek ki, p\u00e9ld\u00e1ul gyullad\u00e1scs\u00f6kkent\u0151, antibakteri\u00e1lis, antioxid\u00e1ns \u00e9s tumorellenes hat\u00e1st. Tanulm\u00e1nyok kimutatt\u00e1k, hogy a fenilpropanoid vegy\u00fcletek, mint p\u00e9ld\u00e1ul a ferulasav, hat\u00e9konyan k\u00e9pesek a hidrog\u00e9n-peroxid, a peroxinitrit \u00e9s a szuperoxid gy\u00f6k\u00f6k elnyel\u00e9s\u00e9re. Ez\u00e9rt a fenti eredm\u00e9nyekb\u0151l arra lehet k\u00f6vetkeztetni, hogy a Shanyinhua olaj antioxid\u00e1ns \u00e9s gyullad\u00e1scs\u00f6kkent\u0151 hat\u00e1ssal rendelkezik.<\/p>\n

Zeng \u00e9s munkat\u00e1rsai meg\u00e1llap\u00edtott\u00e1k, hogy a lonc \u00e9s a hegyi ez\u00fcst vir\u00e1gainak kivonata jelent\u0151sen g\u00e1tolja a xilol \u00e1ltal kiv\u00e1ltott f\u00fclduzzanat m\u00e9rt\u00e9k\u00e9t egerekben, \u00e9s g\u00e1tolja az LPS \u00e1ltal kiv\u00e1ltott gyullad\u00e1sos citokinek felszabadul\u00e1s\u00e1t a RAW 264.7 sejtekben egy in vitro sejtmodellben. Mindkett\u0151 j\u00f3 gyullad\u00e1scs\u00f6kkent\u0151 hat\u00e1ssal rendelkezik in vitro \u00e9s in vivo. Zou \u00e9s munkat\u00e1rsai LPS induk\u00e1lt sejtgyullad\u00e1sos modellt haszn\u00e1ltak a Lonicera japonica k\u00fcl\u00f6nb\u00f6z\u0151 kivonatainak in vitro gyullad\u00e1scs\u00f6kkent\u0151 hat\u00e1s\u00e1nak \u00e9rt\u00e9kel\u00e9s\u00e9re. A Lonicera japonica k\u00fcl\u00f6nb\u00f6z\u0151 kivonatai k\u00fcl\u00f6nb\u00f6z\u0151 m\u00e9rt\u00e9k\u0171 gyullad\u00e1scs\u00f6kkent\u0151 hat\u00e1st mutattak. Ez a tanulm\u00e1ny a Lonicera japonica olaj gyullad\u00e1scs\u00f6kkent\u0151 hat\u00e1s\u00e1t \u00e9rt\u00e9kelte LPS induk\u00e1lt sejtgyullad\u00e1sos modell seg\u00edts\u00e9g\u00e9vel, ami azt jelzi, hogy a Lonicera japonica olaj jelent\u0151s gyullad\u00e1scs\u00f6kkent\u0151 hat\u00e1ssal rendelkezik, \u00e9s d\u00f3zisf\u00fcgg\u0151 mint\u00e1zatot mutat, ami hasonl\u00f3 a kor\u00e1bbi vizsg\u00e1latokhoz.<\/p>\n

Val\u00f3j\u00e1ban gyullad\u00e1scs\u00f6kkent\u0151 k\u00eds\u00e9rletek kimutatt\u00e1k, hogy a Shanyinhua olaj jelent\u0151sen g\u00e1tolja a gyullad\u00e1sos faktorok felszabadul\u00e1s\u00e1t \u00e9s gyullad\u00e1scs\u00f6kkent\u0151 hat\u00e1st fejt ki. A fenti eredm\u00e9nyek azt mutatj\u00e1k, hogy a Shanyinhua-olaj j\u00f3 gyullad\u00e1scs\u00f6kkent\u0151 hat\u00e1s\u00fa term\u00e9szetes hat\u00f3anyag, \u00e9s j\u00f3 fejleszt\u00e9si \u00e9s alkalmaz\u00e1si \u00e9rt\u00e9kkel rendelkezik. A vizsg\u00e1lat antioxid\u00e1ns eredm\u00e9nyei azt mutatj\u00e1k, hogy a Shanyinhua olaj ABTS \u00e9s DPPH szabadgy\u00f6k\u00f6k elnyel\u00e9s\u00e9re val\u00f3 k\u00e9pess\u00e9ge nem er\u0151s, ami az ezzel a m\u00f3dszerrel kivont olaj alacsony antioxid\u00e1ns anyagtartalm\u00e1nak tudhat\u00f3 be. Ez arra is utal, hogy \u00faj extrakci\u00f3s m\u00f3dszereket fejleszthet\u00fcnk tov\u00e1bb. Ez\u00e9rt e tanulm\u00e1ny \u00e1tfog\u00f3 k\u00e9miai elemz\u00e9si eredm\u00e9nyei \u00e9s el\u0151zetes aktivit\u00e1s\u00e9rt\u00e9kel\u00e9se alapot ny\u00fajthat a Shanyinhua \u00e1tfog\u00f3 fejleszt\u00e9s\u00e9hez mind a gy\u00f3gy\u00e1szati, mind az \u00e9tkez\u00e9si alkalmaz\u00e1sokban.<\/p>","protected":false},"excerpt":{"rendered":"

Full component characterization and in vitro anti-inflammatory and antioxidant activities of Shanyinhua oil by GC-MS and LC-MS Mountain Silver Flower is a member of the Lonicera family, with the plant Lonicera macranthoides Hand- Mazz.\u3001 Lonicera hypoglauca Miq., Lonicera hypoglauca Miq., Lonicera hypoglauca Miq., Lonicera hypoglauca Miq., Lonicera hypoglauca Miq., Lonicera hypoglauca Miq., Lonicera hypoglauca Miq […]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"\nFull component characterization and in vitro anti-inflammatory and antioxidant activities of Shanyinhua oil by GC-MS and LC-MS - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/hu\/gc-ms\/\" \/>\n<meta property=\"og:locale\" content=\"hu_HU\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Full component characterization and in vitro anti-inflammatory and antioxidant activities of Shanyinhua oil by GC-MS and LC-MS - 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