{"id":4459,"date":"2024-08-04T14:28:35","date_gmt":"2024-08-04T14:28:35","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=4459"},"modified":"2024-08-04T14:28:35","modified_gmt":"2024-08-04T14:28:35","slug":"natural-low-melting-point-solvent","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/hu\/natural-low-melting-point-solvent\/","title":{"rendered":"Az oleuropein cellul\u00e1z hidrol\u00edzis\u00e9nek term\u00e9szetes eutektikus old\u00f3szerekkel t\u00f6rt\u00e9n\u0151 el\u0151seg\u00edt\u00e9s\u00e9nek vizsg\u00e1lata"},"content":{"rendered":"

Az oleuropein cellul\u00e1z hidrol\u00edzis\u00e9nek term\u00e9szetes eutektikus old\u00f3szerekkel t\u00f6rt\u00e9n\u0151 el\u0151seg\u00edt\u00e9s\u00e9nek vizsg\u00e1lata
\nAz Olea europaea L. az olajfaf\u00e9l\u00e9k (Oleaceae) csal\u00e1dj\u00e1ba \u00e9s az olajfaf\u00e9l\u00e9k (Oleaceae) nemzets\u00e9g\u00e9be tartoz\u00f3 n\u00f6v\u00e9ny. A mediterr\u00e1n t\u00e9rs\u00e9gben fontos olajn\u00f6v\u00e9ny, K\u00edn\u00e1ban pedig Gansu \u00e9s Yunnan az olajbogy\u00f3 f\u0151 term\u0151ter\u00fclete. Az olajfalev\u00e9l az olajfa termeszt\u00e9s\u00e9nek olcs\u00f3 mell\u00e9kterm\u00e9ke. Minden olajf\u00e1r\u00f3l \u00e9vente k\u00f6r\u00fclbel\u00fcl 25 kg levelet kell lev\u00e1gni, \u00e9s a betakar\u00edtott levelek \u00f6sszt\u00f6mege az olajbogy\u00f3 \u00f6sszt\u00f6meg\u00e9nek 10% r\u00e9sz\u00e9t teszi ki. Az olajfalevelek k\u00fcl\u00f6nb\u00f6z\u0151 bioakt\u00edv vegy\u00fcleteket tartalmaznak. Ilyen p\u00e9ld\u00e1ul az oleanolsav, a galagonyasav, az ursolsav, az oleuropein, a hidroxitirozol stb.
\nA kutat\u00e1sok kimutatt\u00e1k, hogy az oleuropein az olajfa minden r\u00e9sz\u00e9ben megtal\u00e1lhat\u00f3, a legnagyobb mennyis\u00e9gben az olajfalevelekben. A sz\u00e1raz olajfalevelekben az oleuropein-tartalom el\u00e9rheti a 10% \u00e9s 17% k\u00f6z\u00f6tti \u00e9rt\u00e9ket. Amint az 1. \u00e1br\u00e1n l\u00e1that\u00f3, az oleuropein hidrol\u00edzissel hidroxitirozol, gl\u00fck\u00f3z \u00e9s oli\u00e9nsav keletkezik. Ezek k\u00f6z\u00fcl a hidroxitir\u00f3zol jobb biol\u00f3giai aktivit\u00e1st mutat, mint az oleuropein. Jelenleg az oleuropein hidrol\u00edzis\u00e9nek m\u00f3dszerei k\u00f6z\u00e9 els\u0151sorban a savas hidrol\u00edzis, a l\u00fagos hidrol\u00edzis \u00e9s az enzimatikus hidrol\u00edzis tartozik. A savas hidrol\u00edzis \u00e9s a l\u00fagos hidrol\u00edzis er\u0151s savakat, er\u0151s b\u00e1zisokat \u00e9s magas h\u0151m\u00e9rs\u00e9klet\u0171 k\u00f6rnyezetet ig\u00e9nyel, szigor\u00fa reakci\u00f3k\u00f6r\u00fclm\u00e9nyek mellett. Az ut\u00f3bbi \u00e9vekben nagy figyelmet kapott az enzimek kataliz\u00e1tork\u00e9nt val\u00f3 felhaszn\u00e1l\u00e1sa a mell\u00e9kterm\u00e9kek biotranszform\u00e1ci\u00f3j\u00e1ban. A hagyom\u00e1nyos sav-b\u00e1zis hidrol\u00edzis m\u00f3dszerekkel \u00f6sszehasonl\u00edtva az oleuropein enzimatikus hidrol\u00edzis\u00e9nek el\u0151nye az enyhe reakci\u00f3k\u00f6r\u00fclm\u00e9nyek \u00e9s a kisebb k\u00f6rnyezetszennyez\u00e9s. Liu \u00e9s munkat\u00e1rsai cellul\u00e1zt haszn\u00e1ltak \u00e9lelmiszer-adal\u00e9kanyagk\u00e9nt glikozidos k\u00f6t\u00e9sek felbont\u00e1s\u00e1ra \u00e9s az oleuropein hidrol\u00edzis\u00e9re, hogy hidroxitirozolt nyerjenek. A cellul\u00e1z termikus instabilit\u00e1sa miatt az enzim optim\u00e1lis h\u0151m\u00e9rs\u00e9klet\u00e9n nem k\u00e9pes folyamatosan magas aktivit\u00e1st fenntartani, ami alacsony enzimatikus hidrol\u00edzis hat\u00e9konys\u00e1got eredm\u00e9nyez. Ez\u00e9rt megpr\u00f3b\u00e1lunk egy \u00faj z\u00f6ld old\u00f3szert tal\u00e1lni a pufferoldat helyettes\u00edt\u00e9s\u00e9re, amely n\u00f6velheti az oleuropein oldhat\u00f3s\u00e1g\u00e1t \u00e9s jav\u00edthatja a cellul\u00e1z stabilit\u00e1s\u00e1t a hossz\u00fa t\u00e1v\u00fa reakci\u00f3kban, ez\u00e1ltal jav\u00edtva az oleuropein enzimatikus hidrol\u00edzis\u00e9nek hat\u00e9konys\u00e1g\u00e1t.
\nA m\u00e9ly eutektikus old\u00f3szerek (DES) homog\u00e9n \u00e9s \u00e1tl\u00e1tsz\u00f3 kever\u00e9kek, amelyek hidrog\u00e9nk\u00f6t\u00e9s-akceptorok (HBA) \u00e9s hidrog\u00e9nk\u00f6t\u00e9s-donorok (HBD) magas h\u0151m\u00e9rs\u00e9kleten t\u00f6rt\u00e9n\u0151 kever\u00e9s\u00e9vel j\u00f6nnek l\u00e9tre, \u00e9s az egyes komponensekhez k\u00e9pest alacsonyabb olvad\u00e1spontokkal rendelkeznek. Kiv\u00e1l\u00f3 fizikai \u00e9s k\u00e9miai tulajdons\u00e1gai, k\u00f6zt\u00fck az alacsony gy\u00fal\u00e9konys\u00e1g, az alacsony ill\u00e9konys\u00e1g, a k\u00f6nny\u0171 elk\u00e9sz\u00edthet\u0151s\u00e9g, a nagy oldhat\u00f3s\u00e1g \u00e9s az enzimekkel val\u00f3 nagyfok\u00fa kompatibilit\u00e1s miatt a DES-t egyre gyakrabban haszn\u00e1lj\u00e1k nem toxikus extrakci\u00f3s \u00e9s old\u00f3szerk\u00e9nt. A legfontosabb dolog az, hogy az enzimek hajlamosak a szerves old\u00f3szerekben val\u00f3 inaktiv\u00e1l\u00f3d\u00e1sra, \u00e9s a DES mint old\u00f3szer megakad\u00e1lyozhatja ezt a jelens\u00e9get. Ez lehet\u0151v\u00e9 teszi azt is, hogy a DES helyettes\u00edtse a szerves old\u00f3szereket \u00e9s inkub\u00e1lja a cellul\u00e1zokat. A term\u00e9szetes HBA-b\u00f3l \u00e9s HBD-b\u0151l \u00e1ll\u00f3 DES-t NADES-nek nevezik, amely biztons\u00e1gosabb \u00e9s z\u00f6ldebb, mint a hagyom\u00e1nyos DES. A NADES sz\u00e9les k\u00f6r\u0171 figyelmet kapott, mint z\u00f6ld old\u00f3szer az enzimkataliz\u00e1lt reakci\u00f3k ter\u00fclet\u00e9n. A NADES-t sikeresen alkalmazt\u00e1k specifikus enzimatikus reakci\u00f3kban, kedvez\u0151 hat\u00e1st gyakorolva mind az enzimaktivit\u00e1sra, mind a stabilit\u00e1sra. A fenti kutat\u00e1si eredm\u00e9nyek azt mutatj\u00e1k, hogy a NADES j\u00f3 old\u00f3szer lehet az oleuropein cellul\u00e1z hidrol\u00edzis\u00e9hez.
\nEbben a tanulm\u00e1nyban hatf\u00e9le NADES-t \u00e1ll\u00edtottak el\u0151 kolin-klorid vagy betain mint HBA \u00e9s k\u00fcl\u00f6nb\u00f6z\u0151 t\u00edpus\u00fa poliolok mint HBD felhaszn\u00e1l\u00e1s\u00e1val, \u00e9s vizsg\u00e1lt\u00e1k az oleuropein cellul\u00e1z hidrol\u00edzis\u00e9re gyakorolt hat\u00e1sukat. A k\u00fcl\u00f6nb\u00f6z\u0151 t\u00edpus\u00fa \u00e9s koncentr\u00e1ci\u00f3j\u00fa NADES-ek \u00e9s monomerjeik enzimatikus hidrol\u00edzissebess\u00e9gre gyakorolt hat\u00e1s\u00e1nak felt\u00e1r\u00e1s\u00e1val egy \u00faj, hat\u00e9kony \u00e9s z\u00f6ld m\u00f3dszert hoztunk l\u00e9tre az oleuropein cellul\u00e1z hidrol\u00edzis\u00e9re.<\/p>\n

\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"Ez a tanulm\u00e1ny a NADES hat\u00e1s\u00e1t vizsg\u00e1lta az oleuropein\u00e1z hidrol\u00edzis\u00e9nek sebess\u00e9g\u00e9re \u00fagy, hogy a pufferoldathoz k\u00fcl\u00f6nb\u00f6z\u0151 t\u00edpus\u00fa \u00e9s koncentr\u00e1ci\u00f3j\u00fa NADES-t adtak. Meg\u00e1llap\u00edtottuk, hogy a NADES megfelel\u0151 t\u00edpus\u00e1nak \u00e9s koncentr\u00e1ci\u00f3j\u00e1nak kiv\u00e1laszt\u00e1sa n\u00f6velheti a cellul\u00e1z stabilit\u00e1s\u00e1t \u00e9s el\u0151seg\u00edtheti az oleuropein\u00e1z hidrol\u00edzis\u00e9t. 50 \u2103 h\u0151m\u00e9rs\u00e9klet \u00e9s pH=5 k\u00f6r\u00fclm\u00e9nyek k\u00f6z\u00f6tt az oleuropein\u00e1z hidrol\u00edzis\u00e9nek sebess\u00e9ge 10% DES-5 (V\/V) old\u00f3szeres rendszerben 3 \u00f3ra hidrol\u00edzis ut\u00e1n 1,7-szerese volt a pufferoldat\u00e9nak; Az enzimatikus hidrol\u00edzis sebess\u00e9ge 36 \u00f3ra hidrol\u00edzis ut\u00e1n 1,5-szerese a pufferoldat\u00e9nak; Az enyhe, hat\u00e9kony \u00e9s k\u00f6rnyezetbar\u00e1t enzimatikus hidrol\u00edzis felt\u00e9telei alapj\u00e1n ez a m\u00f3dszer lehet\u0151v\u00e9 teszi, hogy a cellul\u00e1z hossz\u00fa ideig hat\u00e9konyan hidroliz\u00e1lja az oleuropeint. Ez a tanulm\u00e1ny kolin-kloridot vagy betaint haszn\u00e1lt HBA-k\u00e9nt \u00e9s poliolokat HBD-k\u00e9nt a NADES-ben a cellul\u00e1z stabilit\u00e1s\u00e1nak fokoz\u00e1s\u00e1ra \u00e9s az oleuropein\u00e1z hidrol\u00edzis\u00e9nek el\u0151seg\u00edt\u00e9s\u00e9re, tudom\u00e1nyos alapot biztos\u00edtva az oleuropein\u00e1z hidrol\u00edzis term\u00e9keinek hat\u00e9kony el\u0151\u00e1ll\u00edt\u00e1s\u00e1hoz.<\/p>","protected":false},"excerpt":{"rendered":"

Study on the promotion of cellulase hydrolysis of oleuropein by natural eutectic solvents Olea europaea L. is a plant belonging to the Oleaceae family and the Oleaceae genus. It is an important oil crop in the Mediterranean region, and Gansu and Yunnan are the main planting areas for olives in China. Olive leaves are a […]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"\nStudy on the promotion of cellulase hydrolysis of oleuropein by natural eutectic solvents - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/hu\/natural-low-melting-point-solvent\/\" \/>\n<meta property=\"og:locale\" content=\"hu_HU\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Study on the promotion of cellulase hydrolysis of oleuropein by natural eutectic solvents - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"Study on the promotion of cellulase hydrolysis of oleuropein by natural eutectic solvents Olea europaea L. is a plant belonging to the Oleaceae family and the Oleaceae genus. 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It is an important oil crop in the Mediterranean region, and Gansu and Yunnan are the main planting areas for olives in China. Olive leaves are a […]","og_url":"https:\/\/longchangextracts.com\/hu\/natural-low-melting-point-solvent\/","og_site_name":"China Chemical Manufacturer","article_published_time":"2024-08-04T14:28:35+00:00","og_image":[{"url":"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-64.png"}],"author":"longcha9","twitter_card":"summary_large_image","twitter_misc":{"Szerz\u0151:":"longcha9","Becs\u00fclt olvas\u00e1si id\u0151":"6 perc"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/longchangextracts.com\/natural-low-melting-point-solvent\/","url":"https:\/\/longchangextracts.com\/natural-low-melting-point-solvent\/","name":"Tanulm\u00e1ny az oleuropein cellul\u00e1z hidrol\u00edzis\u00e9nek el\u0151seg\u00edt\u00e9s\u00e9r\u0151l term\u00e9szetes eutektikus old\u00f3szerekkel - K\u00edna K\u00e9miai Gy\u00e1rt\u00f3","isPartOf":{"@id":"https:\/\/longchangextracts.com\/#website"},"primaryImageOfPage":{"@id":"https:\/\/longchangextracts.com\/natural-low-melting-point-solvent\/#primaryimage"},"image":{"@id":"https:\/\/longchangextracts.com\/natural-low-melting-point-solvent\/#primaryimage"},"thumbnailUrl":"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-64.png","datePublished":"2024-08-04T14:28:35+00:00","dateModified":"2024-08-04T14:28:35+00:00","author":{"@id":"https:\/\/longchangextracts.com\/#\/schema\/person\/80933ac6926ad63fea74ec33f8d04869"},"breadcrumb":{"@id":"https:\/\/longchangextracts.com\/natural-low-melting-point-solvent\/#breadcrumb"},"inLanguage":"hu","potentialAction":[{"@type":"ReadAction","target":["https:\/\/longchangextracts.com\/natural-low-melting-point-solvent\/"]}]},{"@type":"ImageObject","inLanguage":"hu","@id":"https:\/\/longchangextracts.com\/natural-low-melting-point-solvent\/#primaryimage","url":"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-64.png","contentUrl":"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/1-64.png"},{"@type":"BreadcrumbList","@id":"https:\/\/longchangextracts.com\/natural-low-melting-point-solvent\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/longchangextracts.com\/"},{"@type":"ListItem","position":2,"name":"Study on the promotion of cellulase hydrolysis of oleuropein by natural eutectic solvents"}]},{"@type":"WebSite","@id":"https:\/\/longchangextracts.com\/#website","url":"https:\/\/longchangextracts.com\/","name":"K\u00edna Vegyi Gy\u00e1rt\u00f3","description":"","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/longchangextracts.com\/?s={search_term_string}"},"query-input":"required name=search_term_string"}],"inLanguage":"hu"},{"@type":"Person","@id":"https:\/\/longchangextracts.com\/#\/schema\/person\/80933ac6926ad63fea74ec33f8d04869","name":"longcha9","image":{"@type":"ImageObject","inLanguage":"hu","@id":"https:\/\/longchangextracts.com\/#\/schema\/person\/image\/","url":"https:\/\/secure.gravatar.com\/avatar\/c1e84ee0759373263198c58e8f338f80?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/c1e84ee0759373263198c58e8f338f80?s=96&d=mm&r=g","caption":"longcha9"}}]}},"_links":{"self":[{"href":"https:\/\/longchangextracts.com\/hu\/wp-json\/wp\/v2\/posts\/4459"}],"collection":[{"href":"https:\/\/longchangextracts.com\/hu\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/longchangextracts.com\/hu\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/longchangextracts.com\/hu\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/longchangextracts.com\/hu\/wp-json\/wp\/v2\/comments?post=4459"}],"version-history":[{"count":1,"href":"https:\/\/longchangextracts.com\/hu\/wp-json\/wp\/v2\/posts\/4459\/revisions"}],"predecessor-version":[{"id":4473,"href":"https:\/\/longchangextracts.com\/hu\/wp-json\/wp\/v2\/posts\/4459\/revisions\/4473"}],"wp:attachment":[{"href":"https:\/\/longchangextracts.com\/hu\/wp-json\/wp\/v2\/media?parent=4459"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/longchangextracts.com\/hu\/wp-json\/wp\/v2\/categories?post=4459"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/longchangextracts.com\/hu\/wp-json\/wp\/v2\/tags?post=4459"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}