{"id":4246,"date":"2024-08-04T08:58:38","date_gmt":"2024-08-04T08:58:38","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=4246"},"modified":"2024-08-04T08:58:38","modified_gmt":"2024-08-04T08:58:38","slug":"chinese-rose","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/hu\/chinese-rose\/","title":{"rendered":"UPLC ujjlenyomat \u00e9s t\u00f6bb komponens mennyis\u00e9gi elemz\u00e9se a r\u00f3zsa standard f\u0151zet\u00e9ben"},"content":{"rendered":"

UPLC ujjlenyomat \u00e9s t\u00f6bb komponens mennyis\u00e9gi elemz\u00e9se a r\u00f3zsa standard f\u0151zet\u00e9ben
\nA r\u00f3zsavir\u00e1g, m\u00e1s n\u00e9ven r\u00f3zsav\u00f6r\u00f6s, csangcsunv\u00f6r\u00f6s stb. a r\u00f3zsaf\u00e9l\u00e9k csal\u00e1dj\u00e1ba tartoz\u00f3 Rosa chinensis Jacq. sz\u00e1r\u00edtott vir\u00e1ga. K\u00edna k\u00fcl\u00f6nb\u00f6z\u0151 r\u00e9gi\u00f3iban \u00fcltetik \u0151ket, \u00e9s eg\u00e9sz \u00e9vben sz\u00fcretelhet\u0151k. Akkor szedik, amikor a vir\u00e1gok kiss\u00e9 kiny\u00edltak, \u00e9s \u00e1rny\u00e9kban vagy alacsony h\u0151m\u00e9rs\u00e9kleten sz\u00e1r\u00edtj\u00e1k. A r\u00f3zsavir\u00e1gok gy\u00f3gy\u00e1szati \u00e9rt\u00e9k\u00e9t el\u0151sz\u00f6r Li Shizhen \"Compendium of Materia Medica\" c\u00edm\u0171 m\u0171v\u00e9ben jegyezt\u00e9k fel. \u00cdze \u00e9des, term\u00e9szete meleg, \u00e9s a m\u00e1j meridi\u00e1nj\u00e1hoz tartozik; a v\u00e9rkering\u00e9st el\u0151seg\u00edt\u0151, a menstru\u00e1ci\u00f3t szab\u00e1lyoz\u00f3 \u00e9s a m\u00e1jat nyugtat\u00f3, depresszi\u00f3t enyh\u00edt\u0151 hat\u00e1sa van. Els\u0151sorban k\u00fcl\u00f6nb\u00f6z\u0151 betegs\u00e9gek kezel\u00e9s\u00e9re haszn\u00e1lj\u00e1k, mint p\u00e9ld\u00e1ul a qi stagn\u00e1l\u00e1sa \u00e9s a v\u00e9r pang\u00e1sa, menstru\u00e1ci\u00f3s zavarok, mellkasi \u00e9s bordat\u00e1gulat \u00e9s f\u00e1jdalom. A r\u00f3zsavir\u00e1g k\u00fcl\u00f6nb\u00f6z\u0151 k\u00e9miai \u00f6sszetev\u0151ket tartalmaz, t\u00f6bbek k\u00f6z\u00f6tt flavonoidokat, fenolsavakat, arom\u00e1s olajokat, tanninokat \u00e9s pigmenteket, amelyek k\u00f6z\u00fcl a flavonoidokat \u00e9s a fenolsavakat tekintik a r\u00f3zsavir\u00e1g f\u0151 gy\u00f3gyhat\u00e1s\u00fa \u00f6sszetev\u0151inek. A flavonoidok a f\u0151 \u00f6sszetev\u0151k, bele\u00e9rtve a kvercetin, a kvercetin, a kaempferol-3-O-ramnoside, a kaempferol, a borosty\u00e1nk\u0151sav, a galluszsav, az etil-gall\u00e1t, a hiperozid, a hesperidin, az izokvercitrin stb. Ezek biol\u00f3giai aktivit\u00e1sukkal rendelkeznek, mint p\u00e9ld\u00e1ul v\u00edrusellenes, antioxid\u00e1ns, immuner\u0151s\u00edt\u0151 \u00e9s r\u00e1kellenes hat\u00e1suk.
\nA hagyom\u00e1nyos k\u00ednai orvosl\u00e1s \u00f6sszet\u00e9tele \u00f6sszetett, \u00e9s farmakol\u00f3giai hat\u00e1sai t\u00f6bb hat\u00f3anyag t\u00f6bb c\u00e9lpontra, \u00fatvonalra \u00e9s \u00e1ltal\u00e1nos rendszerre gyakorolt szinergikus hat\u00e1s\u00e1n alapulnak, amelyek szab\u00e1lyoz\u00f3 hat\u00e1st gyakorolnak a szervezetre. A hagyom\u00e1nyos k\u00ednai orvosl\u00e1s ujjlenyomata a hagyom\u00e1nyos k\u00ednai orvosl\u00e1s k\u00e9miai \u00f6sszetev\u0151inek szisztematikus vizsg\u00e1lat\u00e1n alapul. \u00c1tfog\u00f3an megjelen\u00edti a hagyom\u00e1nyos k\u00ednai gy\u00f3gy\u00e1szat \u00f6sszetev\u0151inek inform\u00e1ci\u00f3it az anyagcsoportok szempontj\u00e1b\u00f3l, t\u00fckr\u00f6zve a hagyom\u00e1nyos k\u00ednai gy\u00f3gy\u00e1szat k\u00e9miai \u00f6sszetev\u0151inek \u00e1ltal\u00e1nos helyzet\u00e9t. Ez egy \u00e1tfog\u00f3, sz\u00e1mszer\u0171s\u00edthet\u0151 \u00e9s hat\u00e9kony technikai eszk\u00f6z. Ez a tanulm\u00e1ny a r\u00f3zsavir\u00e1g standard f\u0151zet 20 t\u00e9tel\u00e9nek ujjlenyomat-spektrum\u00e1t UPLC-m\u00f3dszerrel \u00e9s k\u00e9miai mintafelismer\u00e9si technol\u00f3gi\u00e1val kombin\u00e1lt UPLC-m\u00f3dszerrel \u00e1llap\u00edtotta meg, elemezte \u00e9s felt\u00e1rta a r\u00f3zsavir\u00e1g standard f\u0151zet\u00e9t befoly\u00e1sol\u00f3 f\u0151 jellemz\u0151 komponenseket, \u00e9s t\u00f6bb indik\u00e1tor komponens mennyis\u00e9gi elemz\u00e9s\u00e9t v\u00e9gezte el, annak \u00e9rdek\u00e9ben, hogy referenci\u00e1t ny\u00fajtson a r\u00f3zsavir\u00e1g standard f\u0151zet\u00e9vel kapcsolatos k\u00e9sz\u00edtm\u00e9nyek fejleszt\u00e9s\u00e9hez \u00e9s a min\u0151s\u00e9gi szabv\u00e1nyok kutat\u00e1s\u00e1hoz.<\/p>\n

 <\/p>\n

\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"\"A szabv\u00e1nyos f\u0151zet reprezentativit\u00e1s\u00e1nak biztos\u00edt\u00e1sa \u00e9rdek\u00e9ben ez a tanulm\u00e1ny felm\u00e9r\u00e9st v\u00e9gzett a r\u00f3zsavir\u00e1g nyersanyagok f\u0151 termel\u00e9si ter\u00fcleteinek inform\u00e1ci\u00f3ir\u00f3l. \u00d6sszesen 20 t\u00e9tel mint\u00e1t gy\u0171jt\u00f6ttek, bele\u00e9rtve a helysz\u00edni mint\u00e1kat a f\u0151bb \u00e9s hiteles termel\u00e9si ter\u00fcletekr\u0151l, mint p\u00e9ld\u00e1ul Baoding v\u00e1rosb\u00f3l (Hebei tartom\u00e1ny), Zhoukou v\u00e1rosb\u00f3l (Henan tartom\u00e1ny), Heze v\u00e1rosb\u00f3l (Shandong tartom\u00e1ny), Linyi v\u00e1rosb\u00f3l (Shandong tartom\u00e1ny) \u00e9s Dali prefekt\u00far\u00e1b\u00f3l (Yunnan tartom\u00e1ny). A \"Hagyom\u00e1nyos k\u00ednai gy\u00f3gyszerk\u00e9sz\u00edtm\u00e9nyek min\u0151s\u00e9gellen\u0151rz\u00e9s\u00e9nek \u00e9s szabv\u00e1nyos fejleszt\u00e9s\u00e9nek m\u0171szaki k\u00f6vetelm\u00e9nyei\" \u00e9s az \"Orvosi int\u00e9zm\u00e9nyekben a hagyom\u00e1nyos k\u00ednai gy\u00f3gyszerk\u00e9sz\u00edtm\u00e9nyek f\u0151z\u0151helyis\u00e9g\u00e9nek ir\u00e1ny\u00edt\u00e1si el\u0151\u00edr\u00e1sai\" vez\u00e9relvei szerint a szabv\u00e1nyos f\u0151zet el\u0151kezel\u00e9si m\u00f3dszereit (f\u0151z\u00e9si id\u0151k, v\u00edz hozz\u00e1ad\u00e1sa, f\u0151z\u00e9si id\u0151 \u00e9s egy\u00e9b mutat\u00f3k \u00e9s param\u00e9terek) vizsg\u00e1lj\u00e1k az optim\u00e1lis elk\u00e9sz\u00edt\u00e9si folyamat meghat\u00e1roz\u00e1sa \u00e9rdek\u00e9ben; alacsony h\u0151m\u00e9rs\u00e9kleten t\u00f6rt\u00e9n\u0151 koncentr\u00e1l\u00e1s \u00e9s fagyasztva sz\u00e1r\u00edt\u00e1s seg\u00edts\u00e9g\u00e9vel szabv\u00e1nyos r\u00f3zsasz\u00edn f\u0151zet fagyasztva sz\u00e1r\u00edtott por k\u00e9sz\u00fcl.
\nAz ujjlenyomat-m\u00f3dszer kialak\u00edt\u00e1sa sor\u00e1n vizsg\u00e1lt\u00e1k az extrakci\u00f3s old\u00f3szerek (metanol, 70% metanol, 50% metanol, etanol, 70% etanol, h\u00edg etanol), az extrakci\u00f3s m\u00f3dszerek (ultrahang, reflux) \u00e9s az extrakci\u00f3s id\u0151k (10, 30, 45, 60 perc) hat\u00e1s\u00e1t az ujjlenyomatra. Az extrakci\u00f3 megfelel\u0151s\u00e9g\u00e9t, a stabilit\u00e1st \u00e9s a k\u00f6nny\u0171 kezelhet\u0151s\u00e9get figyelembe v\u00e9ve v\u00e9g\u00fcl az 50% metanolos ultrahangos kezel\u00e9sre esett a v\u00e1laszt\u00e1s 30 percig. Egy di\u00f3dasoros detektorral teljes hull\u00e1mhossz\u00fa (200-400 nm) p\u00e1szt\u00e1z\u00e1st v\u00e9gezt\u00fcnk a standard r\u00f3zsaleves mint\u00e1kon, \u00e9s \u00f6sszehasonl\u00edtottuk a k\u00fcl\u00f6nb\u00f6z\u0151 hull\u00e1mhossz\u00fas\u00e1g\u00fa kromatogramok cs\u00facssz\u00e1m\u00e1t, v\u00e1lasz\u00e9rt\u00e9keit \u00e9s felbont\u00e1s\u00e1t. Az eredm\u00e9nyek azt mutatt\u00e1k, hogy a 254 nm-es detekt\u00e1l\u00e1si hull\u00e1mhossz haszn\u00e1lata nagyobb kromatogr\u00e1fi\u00e1s cs\u00facsok sz\u00e1m\u00e1t \u00e9s nagyobb v\u00e1lasz\u00e9rt\u00e9keket, stabil alapvonalat \u00e9s kevesebb interferenci\u00e1t eredm\u00e9nyezett. Ugyanakkor megvizsg\u00e1ltuk a mobil f\u00e1zis v\u00edzf\u00e1zis\u00e1nak, az oszlop h\u0151m\u00e9rs\u00e9klet\u00e9nek \u00e9s \u00e1raml\u00e1si sebess\u00e9g\u00e9nek kromatogr\u00e1fi\u00e1s felt\u00e9teleit, \u00e9s v\u00e9g\u00fcl meg\u00e1llap\u00edtottuk, hogy 0,2% foszforsavoldatot haszn\u00e1lunk mobil f\u00e1zis v\u00edzf\u00e1zisk\u00e9nt, 0,4 ml\/perc \u00e1raml\u00e1si sebess\u00e9ggel \u00e9s 35 \u2103 oszloph\u0151m\u00e9rs\u00e9klettel.
\nAz ujjlenyomat-spektrumot a k\u00e9miai mintafelismer\u0151 technol\u00f3gi\u00e1val kombin\u00e1lva haszn\u00e1lt\u00e1k a k\u00fcl\u00f6nb\u00f6z\u0151 eredet\u0171 r\u00f3zsavir\u00e1gok standard f\u0151zet\u00e9nek elemz\u00e9s\u00e9re \u00e9s \u00f6sszehasonl\u00edt\u00e1s\u00e1ra. Az eredm\u00e9nyek azt mutatt\u00e1k, hogy a fel\u00fcgyelet n\u00e9lk\u00fcli HCA \u00e9s PCA elemz\u00e9s kimutatta, hogy a k\u00fcl\u00f6nb\u00f6z\u0151 eredet\u0171 mint\u00e1k n\u00e9gy kateg\u00f3ri\u00e1ba sorolhat\u00f3k. Az OPLS-DA elemz\u00e9s \u00f6sszesen 11 k\u00fcl\u00f6nb\u00f6z\u0151 biomarkert azonos\u00edtott, f\u0151k\u00e9nt flavonoidokat \u00e9s fenolsav komponenseket, amelyek fontos szerepet j\u00e1tszottak a k\u00fcl\u00f6nb\u00f6z\u0151 eredet\u0171 r\u00f3zsa standard f\u0151zetek megk\u00fcl\u00f6nb\u00f6ztet\u00e9s\u00e9ben. Ezek min\u0151s\u00e9gellen\u0151rz\u00e9si indik\u00e1tork\u00e9nt szolg\u00e1lhatnak a r\u00f3zsa standard f\u0151zetekre, \u00e9s \u00e1tfog\u00f3bb referenci\u00e1t biztos\u00edtanak a r\u00f3zsaszer\u0171 granul\u00e1tumok nyersanyagforr\u00e1saihoz.
\nEbben a tanulm\u00e1nyban ujjlenyomat-spektrumokat haszn\u00e1ltak klaszterez\u00e9ssel, f\u0151komponens-elemz\u00e9ssel, ortogon\u00e1lis r\u00e9szleges legkisebb n\u00e9gyzetek diszkriminancia-anal\u00edzissel \u00e9s t\u00f6bb indexkomponens-tartalom meghat\u00e1roz\u00e1s\u00e1val kombin\u00e1lva, hogy el\u0151zetes vizsg\u00e1latot v\u00e9gezzenek 20 t\u00e9tel r\u00f3zsa standard f\u0151zet\u00e9nek min\u0151s\u00e9g\u00e9r\u0151l. Ezzel a m\u00f3dszerrel gyorsan ki lehet sz\u0171rni azokat a kulcsfontoss\u00e1g\u00fa kromatogr\u00e1fi\u00e1s cs\u00facsokat, amelyek befoly\u00e1solj\u00e1k a k\u00fcl\u00f6nb\u00f6z\u0151 gy\u00f3gy\u00e1szati anyagt\u00e9telek min\u0151s\u00e9g\u00e9t. K\u00f6z\u00fcl\u00fck a gallikusav, a csersav, a hiperozid, az izoquercitrin, az asztragalozid, a kvercitrin \u00e9s a kvercetin nagy felbont\u00e1s\u00fa \u00e9s referenciaanyag spektr\u00e1lis \u00f6sszehasonl\u00edt\u00e1s\u00e1val azonos\u00edtott\u00e1k. Az eredm\u00e9nyek azt mutatt\u00e1k, hogy a magas fenolsav- \u00e9s flavonoidtartalm\u00fa mint\u00e1kat Henan tartom\u00e1nyb\u00f3l gy\u0171jt\u00f6tt\u00e9k, \u00e9s a HCA- \u00e9s PCA-anal\u00edzisben az S11-S13 kateg\u00f3ri\u00e1kba tartoztak. A legalacsonyabb tartalm\u00fa mint\u00e1kat Yunnan tartom\u00e1nyb\u00f3l gy\u0171jt\u00f6tt\u00e9k, az S1-S6 els\u0151 kateg\u00f3ri\u00e1ba tartoztak. Ez alapul szolg\u00e1lhat a k\u00e9s\u0151bbi r\u00f3zsasz\u00edn standard f\u0151zethez sz\u00fcks\u00e9ges nyersanyaggy\u0171jt\u00e9s eredet\u00e9nek kiv\u00e1laszt\u00e1s\u00e1hoz. Jelenleg a kutat\u00e1sok azt mutatj\u00e1k, hogy a r\u00f3zsavir\u00e1gok f\u0151k\u00e9nt flavonoidokat, flavonoid glikozidokat \u00e9s fenolsavvegy\u00fcleteket tartalmaznak, amelyek biol\u00f3giai aktivit\u00e1suk r\u00e9v\u00e9n antioxid\u00e1ns, v\u00edrusellenes, gombaellenes, r\u00e1kellenes, daganatellenes \u00e9s \u00f6reged\u00e9sg\u00e1tl\u00f3 hat\u00e1s\u00faak. A k\u00e9s\u0151bbi szakaszban m\u00e9lyrehat\u00f3 kutat\u00e1sokat fognak v\u00e9gezni a gy\u00f3gyszerhat\u00e9konys\u00e1g alapj\u00e1n, hogy referenci\u00e1t biztos\u00edtsanak a r\u00f3zsavir\u00e1g standard f\u0151zetek \u00e9s a kapcsol\u00f3d\u00f3 k\u00e9sz\u00edtm\u00e9nyek min\u0151s\u00e9gellen\u0151rz\u00e9s\u00e9hez.<\/p>","protected":false},"excerpt":{"rendered":"

UPLC fingerprint and quantitative analysis of multiple components in rose standard decoction Rose flowers, also known as rose red, Changchun red, etc., are the dried flowers of Rosa chinensis Jacq. in the Rosaceae family. They are planted in various regions of China and can be harvested throughout the year. They are picked when the flowers […]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"\nUPLC fingerprint and quantitative analysis of multiple components in rose standard decoction - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/hu\/chinese-rose\/\" \/>\n<meta property=\"og:locale\" content=\"hu_HU\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"UPLC fingerprint and quantitative analysis of multiple components in rose standard decoction - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"UPLC fingerprint and quantitative analysis of multiple components in rose standard decoction Rose flowers, also known as rose red, Changchun red, etc., are the dried flowers of Rosa chinensis Jacq. in the Rosaceae family. They are planted in various regions of China and can be harvested throughout the year. 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