{"id":3943,"date":"2024-08-03T14:20:47","date_gmt":"2024-08-03T14:20:47","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=3943"},"modified":"2024-08-03T14:20:47","modified_gmt":"2024-08-03T14:20:47","slug":"uplc-q-tof-ms-ms-and-network-pharmacology","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/hu\/uplc-q-tof-ms-ms-and-network-pharmacology\/","title":{"rendered":"A Taro acuminata mellr\u00e1kellenes mechanizmus\u00e1nak felt\u00e1r\u00e1sa az UPLC-Q-TOF-MS \/ MS \u00e9s a h\u00e1l\u00f3zati farmakol\u00f3gia alapj\u00e1n"},"content":{"rendered":"

A Taro acuminata mellr\u00e1kellenes mechanizmus\u00e1nak felt\u00e1r\u00e1sa az UPLC-Q-TOF-MS \/ MS \u00e9s a h\u00e1l\u00f3zati farmakol\u00f3gia alapj\u00e1n
\nVil\u00e1gviszonylatban az eml\u0151r\u00e1k az \u00f6sszes r\u00e1kos megbeteged\u00e9s el\u0151fordul\u00e1si ar\u00e1ny\u00e1nak 10%-\u00e9rt \u00e9s a n\u0151i daganatos hal\u00e1loz\u00e1s 15%-\u00e9rt felel\u0151s. Az Eg\u00e9szs\u00e9g\u00fcgyi Vil\u00e1gszervezet \u00e1ltal k\u00f6zz\u00e9tett legfrissebb, 2020-ig sz\u00f3l\u00f3 glob\u00e1lis r\u00e1kjelent\u00e9s szerint 2018-ban 2,1 milli\u00f3 \u00faj mellr\u00e1kos megbeteged\u00e9s t\u00f6rt\u00e9nt, \u00e9s 27000 ember halt meg mellr\u00e1kban, ami komoly vesz\u00e9lyt jelent az emberi eg\u00e9szs\u00e9gre. Ez\u00e9rt az eml\u0151r\u00e1k hat\u00e9kony kezel\u00e9se k\u00fcl\u00f6n\u00f6sen fontos. A szindr\u00f3m\u00e1k megk\u00fcl\u00f6nb\u00f6ztet\u00e9s\u00e9nek \u00e9s kezel\u00e9s\u00e9nek elm\u00e9lete szerint a hagyom\u00e1nyos k\u00ednai orvosl\u00e1s nyilv\u00e1nval\u00f3 gy\u00f3gy\u00edt\u00f3 hat\u00e1ssal b\u00edr a mellr\u00e1kra a test funkci\u00f3inak, p\u00e9ld\u00e1ul a jin, a jang, a qi, a v\u00e9r, a zsigerek \u00e9s a meridi\u00e1nok be\u00e1ll\u00edt\u00e1s\u00e1val. A hagyom\u00e1nyos k\u00ednai orvosl\u00e1s jav\u00edthatja a test anyagcser\u00e9j\u00e9t, szab\u00e1lyozhatja a beteg test\u00e9nek \u00e1ltal\u00e1nos m\u0171k\u00f6d\u00e9s\u00e9t, \u00e9s cs\u00f6kkentheti az eml\u0151r\u00e1k ki\u00fajul\u00e1s\u00e1t \u00e9s \u00e1tt\u00e9tk\u00e9pz\u0151d\u00e9s\u00e9t a klinikai hat\u00e9konys\u00e1g jav\u00edt\u00e1sa \u00e9rdek\u00e9ben.
\nAz Araceae csal\u00e1dba tartoz\u00f3 Alocassia cuculata (Lour.) Schott gy\u00f6kere \u00e9s sz\u00e1ra hagyom\u00e1nyos n\u00e9pi gy\u00f3gym\u00f3d, amely h\u0151elvon\u00f3, m\u00e9regtelen\u00edt\u0151, duzzanatcs\u00f6kkent\u0151, f\u00e1jdalomcsillap\u00edt\u00f3 hat\u00e1s\u00fa. A modern farmakol\u00f3giai vizsg\u00e1latok kimutatt\u00e1k, hogy a taro k\u00e9pes aktiv\u00e1lni az immunfaktorokat, hogy g\u00e1tolja a k\u00fcl\u00f6nb\u00f6z\u0151 daganatos sejtek, p\u00e9ld\u00e1ul a melanoma aktivit\u00e1s\u00e1t, jav\u00edtja a szervezet immunrendszer\u00e9nek k\u00e9pess\u00e9g\u00e9t, kiv\u00e1ltja a r\u00e1kos sejtek apopt\u00f3zis\u00e1t, \u00e9s daganatellenes hat\u00e1st fejt ki. A kapcsol\u00f3d\u00f3 molekul\u00e1ris mechanizmusai olyan jel\u00e1tviteli utakat foglalnak magukban, mint a MAPK \u00e9s a PI3K Akt. Az irodalomban arr\u00f3l sz\u00e1moltak be, hogy az Alocasia cucullata petr\u00f3leum\u00e9ter frakci\u00f3ja (EAC) k\u00e9pes g\u00e1tolni az eml\u0151r\u00e1k MDA-MB-231 sejtek prolifer\u00e1ci\u00f3j\u00e1t in vitro sejtk\u00eds\u00e9rletekben. A mechanizmus a MAPK \u00fatvonal p-ERK\/ERK expresszi\u00f3j\u00e1nak g\u00e1tl\u00e1s\u00e1val f\u00fcgg \u00f6ssze. Ugyanakkor az Alocasia cucullata petr\u00f3leum\u00e9teres frakci\u00f3ja GC-MS anal\u00edzis r\u00e9v\u00e9n k\u00fcl\u00f6nb\u00f6z\u0151 tel\u00edtetlen zs\u00edrsavakban, p\u00e9ld\u00e1ul linolsavban \u00e9s k\u00fcl\u00f6nb\u00f6z\u0151 fitoszterolokban, p\u00e9ld\u00e1ul \u03b3 - szitoszterolban gazdag. A modern kutat\u00e1sok azt mutatj\u00e1k, hogy a tel\u00edtetlen zs\u00edrsavak, p\u00e9ld\u00e1ul a linolsav \u00e9s a fitoszterolok a Bax\/Bcl-2 szintj\u00e9nek szab\u00e1lyoz\u00e1s\u00e1val k\u00e9pesek az eml\u0151r\u00e1ksejtek apopt\u00f3zis\u00e1t kiv\u00e1ltani. Ez arra utal, hogy a Taro petr\u00f3leum\u00e9ter frakci\u00f3j\u00e1nak mellr\u00e1kellenes mechanizmusa szint\u00e9n a Bax\/Bcl-2 szint szab\u00e1lyoz\u00e1s\u00e1hoz kapcsol\u00f3dhat. Jelenleg azonban a Taro acuminata petr\u00f3leum\u00e9ter r\u00e9sz\u00e9nek k\u00e9miai \u00f6sszet\u00e9tel\u00e9re vonatkoz\u00f3 kutat\u00e1sok csak a GC-MS anal\u00edzisre korl\u00e1toz\u00f3dnak, \u00e9s nincs relev\u00e1ns jelent\u00e9s a Taro acuminata petr\u00f3leum\u00e9ter r\u00e9sz\u00e9nek in vivo mellr\u00e1kellenes hat\u00e1smechanizmus\u00e1r\u00f3l. A hat\u00e9konys\u00e1g\u00e1nak anyagi alapjaira \u00e9s az eml\u0151r\u00e1k elleni in vivo hat\u00e1s mechanizmus\u00e1ra vonatkoz\u00f3 kutat\u00e1sok viszonylag gyeng\u00e9k. A 4T1 sejt\u00e1t\u00fcltet\u00e9ses tumormodell n\u00f6veked\u00e9si \u00e1llapota \u00e9s metaszt\u00e1zis helye j\u00f3l szimul\u00e1lja az emberi el\u0151rehaladott eml\u0151r\u00e1kot, \u00e9s j\u00f3 modell\u00e9 v\u00e1lik az el\u0151rehaladott eml\u0151r\u00e1k gy\u00f3gyszereinek \u00e9rt\u00e9kel\u00e9s\u00e9re. Ez\u00e9rt ebben a tanulm\u00e1nyban a Taro acuminata petr\u00f3leum\u00e9teres r\u00e9sz\u00e9t vett\u00e9k kutat\u00e1si t\u00e1rgynak, \u00e9s a Taro acuminata petr\u00f3leum\u00e9teres r\u00e9sz\u00e9nek k\u00e9miai \u00f6sszet\u00e9tel\u00e9t UPLC-Q-TOF-MS\/MS seg\u00edts\u00e9g\u00e9vel hat\u00e1rozt\u00e1k meg, m\u00edg a Taro acuminata petr\u00f3leum\u00e9teres r\u00e9sz\u00e9nek mellr\u00e1kellenes mechanizmus\u00e1nak molekul\u00e1ris szintj\u00e9nek tiszt\u00e1z\u00e1s\u00e1ra a molekul\u00e1ris dokkol\u00e1si technol\u00f3gi\u00e1val kombin\u00e1lt h\u00e1l\u00f3zati farmakol\u00f3giai strat\u00e9gi\u00e1t haszn\u00e1lt\u00e1k. V\u00e9g\u00fcl a Taro acuminata acuminata petr\u00f3leum\u00e9teres r\u00e9sz\u00e9nek mellr\u00e1kellenes hat\u00e1s\u00e1t in vivo \u00e9rt\u00e9kelt\u00e9k a 4T1 mellr\u00e1kos daganatot hordoz\u00f3 eg\u00e9rmodell seg\u00edts\u00e9g\u00e9vel, \u00e9s igazolt\u00e1k a mellr\u00e1kellenes mechanizmus\u00e1t. Annak \u00e9rdek\u00e9ben, hogy k\u00eds\u00e9rleti referenci\u00e1kat biztos\u00edtsunk a hegyes fark\u00fa taro m\u00e9lyrehat\u00f3 kutat\u00e1s\u00e1hoz \u00e9s fejleszt\u00e9s\u00e9hez.<\/p>\n

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Ebben a tanulm\u00e1nyban az UPLC-Q-TOF-MS\/MS technol\u00f3gi\u00e1t haszn\u00e1lt\u00e1k a taro petr\u00f3leum\u00e9ter frakci\u00f3j\u00e1nak 41 k\u00e9miai \u00f6sszetev\u0151j\u00e9nek elemz\u00e9s\u00e9re. Ugyanakkor 32 mellr\u00e1kellenes hat\u00f3anyagot \u00e9s 556 mellr\u00e1kkal kapcsolatos c\u00e9lpontot elemeztek a Tara acuminata petr\u00f3leum\u00e9terfrakci\u00f3j\u00e1b\u00f3l h\u00e1l\u00f3zati farmakol\u00f3giai strat\u00e9gi\u00e1val. A PPI-h\u00e1l\u00f3zat 40 f\u0151 c\u00e9lpontot, p\u00e9ld\u00e1ul a MAPK1 \u00e9s a Bcl-2-t jelzett el\u0151re, \u00e9s ezek a c\u00e9lpontok f\u0151k\u00e9nt a MAPK feh\u00e9rjecsal\u00e1dban \u00e9s a PI3K feh\u00e9rjecsal\u00e1dban koncentr\u00e1l\u00f3dtak. Tov\u00e1bb\u00e1, a GO biol\u00f3giai folyamatok gazdagod\u00e1si elemz\u00e9se azt mutatta, hogy a Tara acuminata petr\u00f3leum\u00e9ter frakci\u00f3j\u00e1nak mellr\u00e1kellenes biol\u00f3giai folyamata els\u0151sorban a sejt apopt\u00f3zis\u00e1nak \u00e9s m\u00e1s biol\u00f3giai folyamatok negat\u00edv szab\u00e1lyoz\u00e1s\u00e1ban vesz r\u00e9szt. A KEGG jel\u00fatvonal d\u00fas\u00edt\u00e1si elemz\u00e9se azt mutatta, hogy a Tara acuminata petr\u00f3leum\u00e9ter frakci\u00f3j\u00e1nak eml\u0151r\u00e1k elleni c\u00e9lpont d\u00fas\u00edt\u00e1sa f\u0151k\u00e9nt a PI3K-Akt \u00e9s a MAPK jel\u00fatvonalakban volt, amelyek mindkett\u0151 a sejt apopt\u00f3zissal kapcsolatos. A PPI-h\u00e1l\u00f3zat el\u0151re jelezte, hogy a MAPK1, a Bcl-2 \u00e9s m\u00e1s, apopt\u00f3zissal kapcsolatos alapvet\u0151 c\u00e9lpontokat kaptunk, \u00e9s molekul\u00e1ris dokkol\u00e1si technol\u00f3gi\u00e1val tov\u00e1bb igazoltuk, hogy a szterol komponens digitalis glikozid ligand, a zs\u00edrsav komponens linol\u00e9nsav \u00e9s a kumarin komponens 7-hidroxikumarin szabadon (k\u00f6t\u00e9si energia<0 kcal\/mol) tudott k\u00f6t\u0151dni az Amorphophallus senticosus petr\u00f3leum\u00e9ter r\u00e9sz\u00e9nek az eml\u0151r\u00e1khoz kapcsol\u00f3d\u00f3 alapvet\u0151 c\u00e9lpontjaival (p-ERK, Bcl-2, Bax). A fenti eredm\u00e9nyek arra utalnak, hogy a taro petr\u00f3leum\u00e9ter frakci\u00f3j\u00e1nak mellr\u00e1kellenes hat\u00e1sa a t\u00f6bbkomponens\u0171 \u00e9s t\u00f6bb c\u00e9lpontot tartalmaz\u00f3 tulajdons\u00e1gaihoz kapcsol\u00f3dik, \u00e9s mellr\u00e1kellenes mechanizmusa az apopt\u00f3zissal kapcsolatos jel\u00e1tviteli utakhoz, p\u00e9ld\u00e1ul a PI3K Akt \u00e9s a MAPK-hoz kapcsol\u00f3dik.<\/p>\n

A Jianwei taro-t a n\u00e9pi gy\u00f3gy\u00e1szatban gyakran haszn\u00e1lj\u00e1k k\u00fcl\u00f6nb\u00f6z\u0151 r\u00e1kos megbeteged\u00e9sek kezel\u00e9s\u00e9re, \u00e9s a gum\u00f3j\u00e1ban l\u00e9v\u0151 vegy\u00fcletek k\u00e9pesek g\u00e1tolni a k\u00fcl\u00f6nb\u00f6z\u0151 r\u00e1kos sejtek n\u00f6veked\u00e9s\u00e9t. Kutat\u00f3csoportunk kor\u00e1bbi kutat\u00e1sai azt tal\u00e1lt\u00e1k, hogy a taro vizes kivonata k\u00e9pes g\u00e1tolni a mellr\u00e1k n\u00f6veked\u00e9s\u00e9t \u00e9s meghosszabb\u00edtani a mellr\u00e1kos egerek t\u00fal\u00e9l\u00e9si idej\u00e9t az\u00e1ltal, hogy fokozza az eg\u00e9r l\u00e9psejtjeinek aktivit\u00e1s\u00e1t. A sejtk\u00eds\u00e9rlet meg\u00e1llap\u00edtotta, hogy a taro jelent\u0151sen kataliz\u00e1lhatja az immunmonocit\u00e1k makrof\u00e1gokk\u00e1 t\u00f6rt\u00e9n\u0151 differenci\u00e1l\u00f3d\u00e1s\u00e1t, serkentheti a megafagocit\u00e1k m\u0171k\u00f6d\u00e9s\u00e9t, \u00e9s aktiv\u00e1lhatja az immunrendszert a daganatellenes hat\u00e1s el\u00e9r\u00e9se \u00e9rdek\u00e9ben. A taro petr\u00f3leum\u00e9ter frakci\u00f3ja k\u00e9pes g\u00e1tolni az emberi MDA-MB-231 eml\u0151r\u00e1ksejtek n\u00f6veked\u00e9s\u00e9t \u00e9s el\u0151seg\u00edteni a r\u00e1kos sejtek apopt\u00f3zis\u00e1t. A k\u00eds\u00e9rlet eredm\u00e9nyei azt mutatt\u00e1k, hogy a taro petr\u00f3leum\u00e9ter frakci\u00f3ja k\u00e9pes g\u00e1tolni az eml\u0151r\u00e1k n\u00f6veked\u00e9si \u00fctem\u00e9t tumort hordoz\u00f3 egerekben, cs\u00f6kkenti a tumor s\u00faly\u00e1t, \u00e9s a tumor g\u00e1tl\u00e1si ar\u00e1nya koncentr\u00e1ci\u00f3f\u00fcgg\u0151 tendenci\u00e1t mutatott. A l\u00e9p fontos immunszerv az \u00e1llatokban, amely t\u00fckr\u00f6zheti a gy\u00f3gyszerek immunrendszerre gyakorolt hat\u00e1s\u00e1t. A sz\u00e1jon \u00e1t beadott EAC k\u00f6zepes \u00e9s nagy d\u00f3zis\u00fa csoportjaiban az egerek l\u00e9pindexe magasabb volt, \u00e9s szignifik\u00e1ns k\u00fcl\u00f6nbs\u00e9gek mutatkoztak a modellcsoport egerekhez k\u00e9pest, ami azt jelzi, hogy a tar\u00f3 petr\u00f3leum\u00e9ter frakci\u00f3ja jav\u00edthatja a tumort hordoz\u00f3 egerek immunfunkci\u00f3j\u00e1t. A tumorsz\u00f6vet HE-fest\u00e9si eredm\u00e9nyei azt mutatt\u00e1k, hogy a modellcsoportos egerek tumorsejtjei s\u0171r\u0171n n\u00f6vekedtek, m\u00edg az 5-fluorouracil csoportban \u00e9s a tar\u00f3val kezelt csoportban a sejtek laza elrendez\u0151d\u00e9se, elmos\u00f3dott sejtkont\u00farok \u00e9s a tumorsejtek nyilv\u00e1nval\u00f3 nekrotikus ter\u00fcletei voltak, ami a tumorsejtek membr\u00e1nreped\u00e9s\u00e9re utal. A taro petr\u00f3leum\u00e9teres helye k\u00e9pes volt a tumorsejtek apopt\u00f3zis\u00e1t kiv\u00e1ltani.<\/p>\n

Sz\u00e1mos tanulm\u00e1ny kimutatta, hogy a gyullad\u00e1s sz\u00e1mos betegs\u00e9get k\u00f6zvet\u00edthet, bele\u00e9rtve a sz\u00edv- \u00e9s \u00e9rrendszeri betegs\u00e9geket, a r\u00e1kot, a cukorbetegs\u00e9get, az \u00edz\u00fcleti gyullad\u00e1st, az Alzheimer-k\u00f3rt stb. A kiv\u00e1lt\u00f3 t\u00e9nyez\u0151k tart\u00f3s jelenl\u00e9te olyan gyullad\u00e1shoz vezethet, amelyet nem lehet megsz\u00fcntetni, \u00e9s a gyullad\u00e1s kr\u00f3nikuss\u00e1 v\u00e1lhat, ami sejtmut\u00e1ci\u00f3kat \u00e9s sejtburj\u00e1nz\u00e1st eredm\u00e9nyez, ami ink\u00e1bb kedvez a r\u00e1k kialakul\u00e1s\u00e1nak \u00e9s fejl\u0151d\u00e9s\u00e9nek. A tumor nekr\u00f3zis faktor (TNF) az egyik fontos proinflammatorikus citokin. Az eml\u0151r\u00e1kkal kapcsolatos mikrok\u00f6rnyezetet kr\u00f3nikus gyullad\u00e1s jellemzi. A TNF - \u03b1 el\u0151seg\u00edtheti a daganatok kialakul\u00e1s\u00e1t \u00e9s fejl\u0151d\u00e9s\u00e9t az\u00e1ltal, hogy serkenti az \u00f6sztrog\u00e9n metabolizmust a tumorsejtekben \u00e9s a k\u00f6rnyez\u0151 stromasejtekben, valamint felhalmozza az \u00f6sztrog\u00e9nt. Vizsg\u00e1latok azt tal\u00e1lt\u00e1k, hogy az exog\u00e9n TNF - \u03b1 nem k\u00e9pes tumorsejt apopt\u00f3zist induk\u00e1lni MDAMB-468, SK-BR3 \u00e9s MDA-MB-231 sejtekben, m\u00edg az endog\u00e9n TNF - \u03b1 v\u00e9d\u0151, antiapoptotikus aktivit\u00e1ssal rendelkezik HS578T sejtekben. A pro-inflammatorikus citokin interleukin-1 \u03b2 (IL-1 \u03b2) f\u0151 forr\u00e1sai k\u00f6z\u00e9 tartoznak a myeloid sejtek, mint az MDSC-k, makrof\u00e1gok \u00e9s DC-k, amelyek szerepet j\u00e1tszanak a tumor n\u00f6veked\u00e9s\u00e9nek el\u0151seg\u00edt\u00e9s\u00e9ben vagy a tumorellenes immunfelismer\u00e9s fokoz\u00e1s\u00e1ban a tumor mikrok\u00f6rnyezet\u00e9ben. Kimutatt\u00e1k, hogy az IL-1 \u03b2 el\u0151seg\u00edti az MDSC-k felhalmoz\u00f3d\u00e1s\u00e1t a tumor mikrok\u00f6rnyezet\u00e9ben l\u00e9v\u0151 \u00e9retlen myeloid sejtekben, ez\u00e1ltal el\u0151seg\u00edtve a tumor progresszi\u00f3j\u00e1t. Ebben a vizsg\u00e1latban az ELISA-kit kimutat\u00e1s\u00e1val meg\u00e1llap\u00edtott\u00e1k, hogy a modellcsoporthoz k\u00e9pest a TNF - \u03b1 \u00e9s az IL-1 \u03b2 szintje a pozit\u00edv gy\u00f3gyszercsoport \u00e9s a k\u00fcl\u00f6nb\u00f6z\u0151 koncentr\u00e1ci\u00f3j\u00fa tar\u00f3val kezelt csoportok sz\u00e9rum\u00e1ban cs\u00f6kkent, \u00e9s a nagy d\u00f3zis\u00fa csoportban volt a legalacsonyabb. Ez\u00e9rt felt\u00e9telezhet\u0151, hogy a taro petr\u00f3leum\u00e9ter-frakci\u00f3ja egerek tumorbeolt\u00e1s\u00e1t k\u00f6vet\u0151en a pro-inflammatorikus citokinszintek cs\u00f6kkent\u00e9se r\u00e9v\u00e9n g\u00e1tolhatja a tumor kialakul\u00e1s\u00e1t. \u00d6sszefoglalva, a Taro acuminata petr\u00f3leum\u00e9ter frakci\u00f3ja jelent\u0151s mellr\u00e1kellenes hat\u00e1ssal rendelkezik in vivo.<\/p>\n

A MAPK egy szerin-protein kin\u00e1z, amely az endog\u00e9n apoptotikus \u00fatvonalhoz tartozik. Az ERK, a JNK \u00e9s a p38 az eml\u0151s\u00f6kben felfedezett h\u00e1rom legfontosabb jel\u00e1tviteli \u00fatvonal, amelyek k\u00fcl\u00f6nb\u00f6z\u0151 aktiv\u00e1ci\u00f3s \u00e1llapotokkal rendelkeznek. Aktiv\u00e1l\u00e1s ut\u00e1n r\u00e9szt vesznek a k\u00fcl\u00f6nb\u00f6z\u0151 n\u00f6veked\u00e9si faktorok, citokinek \u00e9s hormonreceptorok jel\u00e1tviteli hat\u00e1saiban, szab\u00e1lyozva a sejtek n\u00f6veked\u00e9s\u00e9t \u00e9s differenci\u00e1l\u00f3d\u00e1s\u00e1t. Ebben a k\u00eds\u00e9rleti vizsg\u00e1latban nem volt szignifik\u00e1ns k\u00fcl\u00f6nbs\u00e9g a MAPK-k jel\u00e1tviteli \u00fatvonalhoz kapcsol\u00f3d\u00f3 feh\u00e9rj\u00e9k, ERK, JNK, p38, p-JNK \u00e9s p-p38 feh\u00e9rj\u00e9k szintj\u00e9ben, \u00e9s a p-ERK feh\u00e9rje kifejez\u0151d\u00e9se cs\u00f6kkent, ami arra utal, hogy a Taro petr\u00f3leum\u00e9ter r\u00e9sze eml\u0151r\u00e1kellenes szerepet j\u00e1tszhat a p-ERK \/ ERK kifejez\u0151d\u00e9s\u00e9nek g\u00e1tl\u00e1s\u00e1val, ami \u00f6sszhangban van a kor\u00e1bbi in vitro vizsg\u00e1latok eredm\u00e9nyeivel \u00e9s a kapcsol\u00f3d\u00f3 \u00fatvonalak h\u00e1l\u00f3zati farmakol\u00f3giai el\u0151rejelz\u00e9s\u00e9vel. Ezenk\u00edv\u00fcl az EAC n\u00f6velheti a Bax\/Bcl-2 feh\u00e9rje expresszi\u00f3j\u00e1t \u00e9s az apopt\u00f3zis g\u00e9n Bax\/Bcl-2 mRNS szintj\u00e9t, ami arra utal, hogy az EAC eml\u0151r\u00e1kellenes szerepet j\u00e1tszik a tumorsejtek apopt\u00f3zis\u00e1nak el\u0151seg\u00edt\u00e9s\u00e9vel.
\n\u00d6sszefoglalva, a taro petr\u00f3leum\u00e9ter frakci\u00f3ja k\u00e9pes g\u00e1tolni a 4T1 eml\u0151r\u00e1kot hordoz\u00f3 egerek daganatos n\u00f6veked\u00e9s\u00e9t. Az eml\u0151r\u00e1k elleni hat\u00e1sa t\u00f6bbkomponens\u0171 \u00e9s t\u00f6bb c\u00e9lpontot \u00e9rint\u0151 tulajdons\u00e1gaival f\u00fcgg \u00f6ssze. Mechanizmusa \u00f6sszef\u00fcgghet a p-ERK\/ERK expresszi\u00f3 lefel\u00e9 ir\u00e1nyul\u00f3 szab\u00e1lyoz\u00e1s\u00e1val, a Bax\/Bcl-2 expresszi\u00f3 n\u00f6veked\u00e9s\u00e9vel, a Bax\/Bcl-2 mRNS szintj\u00e9nek n\u00f6veked\u00e9s\u00e9vel \u00e9s a tumorsejt apopt\u00f3zis el\u0151seg\u00edt\u00e9s\u00e9vel. Ez a tanulm\u00e1ny elm\u00e9leti hivatkoz\u00e1si alapot ny\u00fajt a Taro acuminata klinikai alkalmaz\u00e1s\u00e1hoz az eml\u0151r\u00e1k kezel\u00e9s\u00e9ben, de a hat\u00e9konys\u00e1g, a c\u00e9lpont \u00e9s a mechanizmus m\u00e9lyebb anyagi alapjait tov\u00e1bb kell vizsg\u00e1lni.<\/p>","protected":false},"excerpt":{"rendered":"

Exploring the Anti breast cancer Mechanism of Taro acuminata Based on UPLC-Q-TOF-MS\/MS and Network Pharmacology Globally, breast cancer accounts for 10% of the incidence rate of all cancers and 15% of female tumor related mortality. The latest Global Cancer Report 2020 published by the World Health Organization shows that 2.1 million new breast cancer cases […]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"\nExploring the Anti breast cancer Mechanism of Taro acuminata Based on UPLC-Q-TOF-MS\/MS and Network Pharmacology - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/hu\/uplc-q-tof-ms-ms-and-network-pharmacology\/\" \/>\n<meta property=\"og:locale\" content=\"hu_HU\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Exploring the Anti breast cancer Mechanism of Taro acuminata Based on UPLC-Q-TOF-MS\/MS and Network Pharmacology - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"Exploring the Anti breast cancer Mechanism of Taro acuminata Based on UPLC-Q-TOF-MS\/MS and Network Pharmacology Globally, breast cancer accounts for 10% of the incidence rate of all cancers and 15% of female tumor related mortality. 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