{"id":3592,"date":"2024-08-02T15:24:12","date_gmt":"2024-08-02T15:24:12","guid":{"rendered":"https:\/\/longchangextracts.com\/?p=3592"},"modified":"2024-08-02T15:24:12","modified_gmt":"2024-08-02T15:24:12","slug":"uplc-q-tof-ms-extraction-of-fructus-psorale","status":"publish","type":"post","link":"https:\/\/longchangextracts.com\/hu\/uplc-q-tof-ms-fructus-psorale-extrakcioja\/","title":{"rendered":"A Fructus Psorale alkoholos kivonat\u00e1nak k\u00e9miai \u00f6sszet\u00e9tel\u00e9nek elemz\u00e9se \u00e9s a melanin termel\u0151d\u00e9s\u00e9t el\u0151seg\u00edt\u0151 mechanizmus el\u0151zetes felt\u00e1r\u00e1sa UPLC-Q-TOF-MS m\u00f3dszerrel"},"content":{"rendered":"<p>A Fructus Psorale alkoholos kivonat\u00e1nak k\u00e9miai \u00f6sszet\u00e9tel\u00e9nek elemz\u00e9se \u00e9s a melanin termel\u0151d\u00e9s\u00e9t el\u0151seg\u00edt\u0151 mechanizmus el\u0151zetes felt\u00e1r\u00e1sa UPLC-Q-TOF-MS m\u00f3dszerrel<br \/>\nA vitiligo egy gyakori klinikai, lokaliz\u00e1lt depigment\u00e1ci\u00f3s b\u0151rbetegs\u00e9g, amelynek el\u0151fordul\u00e1si ar\u00e1nya vil\u00e1gszerte k\u00f6r\u00fclbel\u00fcl 0,5%~2%. K\u00f6nnyen diagnosztiz\u00e1lhat\u00f3 \u00e9s nehezen kezelhet\u0151, magas el\u0151fordul\u00e1si ar\u00e1ny \u00e9s hossz\u00fa lefoly\u00e1s\u00fa betegs\u00e9g, amely negat\u00edv hat\u00e1ssal van a betegek fizikai \u00e9s ment\u00e1lis eg\u00e9szs\u00e9g\u00e9re. A Psoralea a Psoralea corylifolia L. h\u00fcvelyes n\u00f6v\u00e9ny sz\u00e1r\u00edtott \u00e9s \u00e9rett term\u00e9se, amelyet a hagyom\u00e1nyos k\u00ednai orvosl\u00e1sban els\u0151sorban a mereved\u00e9si zavarok, az \u00e9jszakai emisszi\u00f3, a h\u00e1t- \u00e9s t\u00e9rdf\u00e1jdalom, valamint a vese jang hi\u00e1ny\u00e1b\u00f3l ered\u0151 zih\u00e1l\u00e1s kezel\u00e9s\u00e9re haszn\u00e1lnak. Helyileg is alkalmazhat\u00f3 vitiligo \u00e9s alopecia areata kezel\u00e9s\u00e9re. Nemcsak haz\u00e1nkban ismert a pikkelys\u00f6m\u00f6r haszn\u00e1lata a vitiligo kezel\u00e9s\u00e9re, hanem a d\u00e9lkelet-\u00e1zsiai orsz\u00e1gokban, p\u00e9ld\u00e1ul Jap\u00e1nban, D\u00e9l-Kore\u00e1ban, Indi\u00e1ban stb. is vannak feljegyz\u00e9sek (ismert \"Babchi magok\", \"Bakuchi por\", \"Bakuchiol\" stb. n\u00e9ven). A vitiligo psoral\u00e9nnel t\u00f6rt\u00e9n\u0151 kezel\u00e9s\u00e9re k\u00fcl\u00f6nb\u00f6z\u0151 adagol\u00e1si form\u00e1k is l\u00e9teznek, bele\u00e9rtve a f\u0151zeteket, tinkt\u00far\u00e1kat \u00e9s injekci\u00f3kat (Z41022361 nemzeti orvosi szabv\u00e1ny). B\u00e1r a vitiligo psoral\u00e9nnel t\u00f6rt\u00e9n\u0151 kezel\u00e9s\u00e9r\u0151l sz\u00e1mos irodalmi adat \u00e1ll rendelkez\u00e9sre, a vitiligo kezel\u00e9s\u00e9nek hat\u00e1smechanizmusa \u00e9s anyagi alapja nem teljesen ismert. Ez\u00e9rt ez a cikk ultra-nagy teljes\u00edtm\u00e9ny\u0171 folyad\u00e9kkromatogr\u00e1fi\u00e1s kvadrupolos rep\u00fcl\u00e9si id\u0151 t\u00f6megspektrometri\u00e1s (UPLC-Q-TOF-MS) technol\u00f3gi\u00e1t haszn\u00e1l a Psoraleae Fructus alkoholos kivonat (PFE) k\u00e9miai \u00f6sszet\u00e9tel\u00e9nek tanulm\u00e1nyoz\u00e1s\u00e1ra; A melanin termel\u00e9s el\u0151seg\u00edt\u00e9s\u00e9re zebrafisheken v\u00e9gzett k\u00eds\u00e9rletekkel a PFE melanin termel\u00e9sre gyakorolt hat\u00e1s\u00e1t vizsg\u00e1lt\u00e1k. Val\u00f3s idej\u0171 fluoreszcens kvantitat\u00edv polimer\u00e1z l\u00e1ncreakci\u00f3t (qRT PCR) haszn\u00e1ltunk a tirozin\u00e1z (Tyr), a tirozin\u00e1zzal kapcsolatos feh\u00e9rje 1 (Tyrp1) \u00e9s a tirozin\u00e1zzal kapcsolatos feh\u00e9rje 2 (Tyrp2) mRNS expresszi\u00f3s szintj\u00e9nek kimutat\u00e1s\u00e1ra a PFE-vel, pszoral\u00e9nnel (PSO) \u00e9s izoszoral\u00e9nnel (IPSO) kezelt B16-F10 eg\u00e9r melanomasejtekben. A vizsg\u00e1lat c\u00e9lja a PFE feh\u00e9r melanomasejtekre gyakorolt potenci\u00e1lis ter\u00e1pi\u00e1s hat\u00e1sainak tov\u00e1bbi felt\u00e1r\u00e1sa. Az akt\u00edv vegy\u00fcletek \u00e9s a vitiligo c\u00e9lpontjai megteremtik az alapot.<\/p>\n<p>A vitiligo a melanocit\u00e1k k\u00e1rosod\u00e1sa \u00e1ltal okozott, helyileg feh\u00e9r folt a b\u0151r\u00f6n, amely sz\u00e9les k\u00f6rben elismert, neh\u00e9z \u00e9s makacs betegs\u00e9g. Etiol\u00f3gi\u00e1ja \u00e9s patogenezise eddig nem teljesen tiszt\u00e1zott. A kutat\u00e1sok kimutatt\u00e1k, hogy a b\u0151r pigment\u00e1ci\u00f3ja szorosan \u00f6sszef\u00fcgg a melanocit\u00e1k h\u00e1rom f\u0151 anyagcsere-folyamat\u00e1val az epidermiszben, bele\u00e9rtve a melanocita prolifer\u00e1ci\u00f3t, a TYR szint\u00e9zis\u00e9t \u00e9s aktiv\u00e1l\u00e1s\u00e1t, valamint a melanin szint\u00e9zis\u00e9t, valamint a melanin \u00e1tvitel\u00e9nek \u00fatj\u00e1t a melanosz\u00f3m\u00e1kb\u00f3l a keratinocit\u00e1kba. A melaninszint\u00e9zis egy \u00f6sszetett reakci\u00f3, amely a melanocit\u00e1kon bel\u00fcl zajlik, \u00e9s amelyet t\u00f6bb t\u00e9nyez\u0151 szab\u00e1lyoz. A TYR a melaninszint\u00e9zis sebess\u00e9gkorl\u00e1toz\u00f3 enzimje, aktivit\u00e1s\u00e1t a TYRP1 \u00e9s a TYRP2 szab\u00e1lyozza, \u00edgy a melaninszint\u00e9zis kulcsfontoss\u00e1g\u00fa szab\u00e1lyoz\u00f3 t\u00e9nyez\u0151je.<br \/>\nA vitiligo patogenezise \u00e9s klinikai megnyilv\u00e1nul\u00e1sai alapj\u00e1n a kutat\u00f3k az elm\u00falt \u00e9vekben sz\u00e1mos \u00e1llatmodellt fejlesztettek ki, mint p\u00e9ld\u00e1ul k\u00e9miai depigment\u00e1ci\u00f3s szer indukci\u00f3, melanocita stressz indukci\u00f3, immunindukci\u00f3, exog\u00e9n T-sejt indukci\u00f3, transzgenikus, melanocita lev\u00e1l\u00e1s indukci\u00f3 stb. Az egerek a f\u0151 v\u00e1laszt\u00e1s az \u00e1llatok sz\u00e1m\u00e1ra. A kutat\u00e1sok szerint az emberi feh\u00e9rjek\u00f3dol\u00f3 g\u00e9nek 71,4%-je \u00e9s a patog\u00e9n g\u00e9nek 82%-je k\u00f6zvetlen\u00fcl homol\u00f3g a zebrahalakkal, bele\u00e9rtve sz\u00e1mos, az emberi b\u0151rbetegs\u00e9gekkel kapcsolatos g\u00e9nt. A zebrahal-l\u00e1rv\u00e1k \u00e1tl\u00e1tsz\u00f3ak, \u00e9s b\u0151r\u00fck \u00e1ltal\u00e1nos \u00e9s sejtszerkezete hasonl\u00f3 az eml\u0151s\u00f6k b\u0151r\u00e9hez. Az ember\u00e9hez hasonl\u00f3 melanocit\u00e1kkal \u00e9s melanosz\u00f3m\u00e1kkal rendelkeznek, \u00e9s a melanocit\u00e1k k\u00f6nnyen megfigyelhet\u0151k az egyedfejl\u0151d\u00e9s sor\u00e1n. Ez teszi a zebrahalat sz\u00e9les k\u00f6rben haszn\u00e1lt \u00e1llatmodell\u00e9 a pigment\u00e1ci\u00f3 kutat\u00e1s\u00e1ban, bele\u00e9rtve az olyan betegs\u00e9geket, mint a vitiligo \u00e9s a melanoma. Mivel azonban a vitiligo egy \u00f6sszetett, multifaktori\u00e1lis betegs\u00e9g, jelenleg nincs olyan \u00e1llatmodell, amely teljes m\u00e9rt\u00e9kben szimul\u00e1lni tudn\u00e1 a hum\u00e1n vitiligo el\u0151fordul\u00e1s\u00e1t \u00e9s fejl\u0151d\u00e9si folyamat\u00e1t. Ez\u00e9rt a vitiligo \u00e1llati modelljeit m\u00e9g tov\u00e1bb kell kutatni.<br \/>\nA PTU a TYR g\u00e1tl\u00f3ja, amelyet sz\u00e9les k\u00f6rben haszn\u00e1lnak a pigment\u00e1ci\u00f3 blokkol\u00e1s\u00e1ra \u00e9s az optikai \u00e1tl\u00e1that\u00f3s\u00e1g jav\u00edt\u00e1s\u00e1ra zebrafikus embri\u00f3kban. Tanulm\u00e1nyok kimutatt\u00e1k, hogy a PTU-oldat haszn\u00e1lata a halv\u00edz helyett a zebrahal b\u00e9l embriogenezist k\u00f6vet\u0151en (5,5 hpf) hat\u00e9konyan g\u00e1tolja a melanin termel\u0151d\u00e9s\u00e9t \u00e9s fenntartja a zebrahalak \u00e1tl\u00e1tsz\u00f3s\u00e1g\u00e1t. A k\u00eds\u00e9rlet eredm\u00e9nyei azt mutatj\u00e1k, hogy a PTU-modellez\u00e9s ut\u00e1n 18 \u00f3r\u00e1n \u00e1t tart\u00f3 PFE-vel t\u00f6rt\u00e9n\u0151 zebrahal kezel\u00e9s ut\u00e1n, az embri\u00f3teny\u00e9szt\u0151 k\u00f6zeggel kezelt csoporthoz k\u00e9pest a PFE felgyors\u00edthatja a melanin regener\u00e1l\u00f3d\u00e1s\u00e1t a zebrahalivad\u00e9kok szem\u00e9ben, has\u00e1ban, gerinc\u00e9ben \u00e9s fark\u00e1ban, ami \u00f6sszhangban van a psoralen klinikai alkalmaz\u00e1s\u00e1val a vitiligo kezel\u00e9s\u00e9ben.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3593\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802231931.png\" alt=\"\" width=\"540\" height=\"302\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3594\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802231938.png\" alt=\"\" width=\"1070\" height=\"384\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802231938.png 1070w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802231938-768x276.png 768w\" sizes=\"(max-width: 1070px) 100vw, 1070px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3595\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802231941.png\" alt=\"\" width=\"1072\" height=\"652\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802231941.png 1072w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802231941-768x467.png 768w\" sizes=\"(max-width: 1072px) 100vw, 1072px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3596\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802231944.png\" alt=\"\" width=\"1076\" height=\"734\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802231944.png 1076w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802231944-768x524.png 768w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802231944-1055x720.png 1055w\" sizes=\"(max-width: 1076px) 100vw, 1076px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3597\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802231948.png\" alt=\"\" width=\"1096\" height=\"1228\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802231948.png 1096w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802231948-768x860.png 768w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802231948-643x720.png 643w\" sizes=\"(max-width: 1096px) 100vw, 1096px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3598\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802231951.png\" alt=\"\" width=\"1082\" height=\"1240\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802231951.png 1082w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802231951-768x880.png 768w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802231951-628x720.png 628w\" sizes=\"(max-width: 1082px) 100vw, 1082px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3599\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802231954.png\" alt=\"\" width=\"1086\" height=\"1246\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802231954.png 1086w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802231954-768x881.png 768w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802231954-628x720.png 628w\" sizes=\"(max-width: 1086px) 100vw, 1086px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3600\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802231957.png\" alt=\"\" width=\"1080\" height=\"1248\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802231957.png 1080w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802231957-768x887.png 768w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802231957-623x720.png 623w\" sizes=\"(max-width: 1080px) 100vw, 1080px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3601\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802232000.png\" alt=\"\" width=\"1090\" height=\"1272\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802232000.png 1090w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802232000-768x896.png 768w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802232000-617x720.png 617w\" sizes=\"(max-width: 1090px) 100vw, 1090px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3602\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802232003.png\" alt=\"\" width=\"1064\" height=\"922\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802232003.png 1064w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802232003-768x666.png 768w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802232003-831x720.png 831w\" sizes=\"(max-width: 1064px) 100vw, 1064px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3603\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802232007.png\" alt=\"\" width=\"1048\" height=\"1034\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802232007.png 1048w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802232007-768x758.png 768w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802232007-730x720.png 730w\" sizes=\"(max-width: 1048px) 100vw, 1048px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3604\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802232010.png\" alt=\"\" width=\"1048\" height=\"450\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802232010.png 1048w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802232010-768x330.png 768w\" sizes=\"(max-width: 1048px) 100vw, 1048px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3605\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802232013.png\" alt=\"\" width=\"1036\" height=\"500\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802232013.png 1036w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802232013-768x371.png 768w\" sizes=\"(max-width: 1036px) 100vw, 1036px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3606\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802232017.png\" alt=\"\" width=\"1046\" height=\"824\" srcset=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802232017.png 1046w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802232017-768x605.png 768w, https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802232017-914x720.png 914w\" sizes=\"(max-width: 1046px) 100vw, 1046px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3607\" src=\"https:\/\/longchangextracts.com\/wp-content\/uploads\/2024\/08\/\u5fae\u4fe1\u56fe\u7247_20240802232019.png\" alt=\"\" width=\"554\" height=\"460\" \/>A K\u00ednai Gy\u00f3gyszerk\u00f6nyv \u00e9s az \u0151si \u00e9s modern gy\u00f3gyn\u00f6v\u00e9nyk\u00f6nyvek szerint a Fructus Psorale hagyom\u00e1nyos k\u00ednai gy\u00f3gyszer, amelynek bizony\u00edtott hat\u00e9konys\u00e1ga a vitiligo kezel\u00e9s\u00e9ben. A legt\u00f6bb eg\u00e9szs\u00e9g\u00fcgyi int\u00e9zm\u00e9ny a Fructus Psorale-t tinkt\u00far\u00e1kban haszn\u00e1lja, 30% \u00e9s 100% k\u00f6z\u00f6tti etanolkoncentr\u00e1ci\u00f3val. Ez\u00e9rt a szakirodalom \u00e9s a gyakorlati alkalmaz\u00e1sok alapj\u00e1n ez a tanulm\u00e1ny 65% etanolt v\u00e1lasztott extrakci\u00f3s reagensnek. Ez a tanulm\u00e1ny UPLC-Q-TOF-MS technol\u00f3gi\u00e1t haszn\u00e1lt a PFE k\u00e9miai \u00f6sszetev\u0151inek azonos\u00edt\u00e1s\u00e1ra. \u00d6sszesen 47 k\u00e9miai komponenst azonos\u00edtottak, f\u0151k\u00e9nt flavonoidokat (27) \u00e9s kumarinokat (10). Az azonos\u00edtott kumarin k\u00e9miai komponensek PSO \u00e9s IPSO lehetnek a f\u0151 hat\u00e9kony k\u00e9miai komponensek a vitiligo kezel\u00e9s\u00e9ben. A benzofur\u00e1nok (mint a pszoral\u00e9n \u00e9s az izopszoral\u00e9n) a szervezetben PSO-v\u00e1 \u00e9s IPSO-v\u00e1 is \u00e1talakulhatnak, hogy kifejts\u00e9k hat\u00e1sukat. A PSO \u00e9s az IPSO a k\u00ednai gy\u00f3gyszerk\u00f6nyvben meghat\u00e1rozott csontzs\u00edr gy\u00f3gy\u00e1szati anyagok min\u0151s\u00e9gellen\u0151rz\u00e9si \u00f6sszetev\u0151i is. Ez\u00e9rt a PFE-t \u00e9s ezt a k\u00e9t k\u00e9miai komponenst v\u00e1lasztottuk a sejtk\u00eds\u00e9rletekhez. A hat\u00e1smechanizmus el\u0151zetes felt\u00e1r\u00e1sa azt mutatta, hogy a qRT PCR eredm\u00e9nyek azt mutatt\u00e1k, hogy a kontrollcsoporthoz k\u00e9pest a PFE jelent\u0151sen n\u00f6velte a Tyr \u00e9s Tyrp2 mRNS expresszi\u00f3j\u00e1t a B16-F10 sejtekben, m\u00edg a t\u00f6bbi kezel\u00e9si csoport k\u00fcl\u00f6nb\u00f6z\u0151 m\u00e9rt\u00e9kben, statisztikai k\u00fcl\u00f6nbs\u00e9gekkel tudta n\u00f6velni a Tyr, Tyrp1 \u00e9s Tyrp2 mRNS expresszi\u00f3j\u00e1t, ami azt jelzi, hogy a PSO, az IPSO \u00e9s a Fructus Psorale-ban tal\u00e1lhat\u00f3 m\u00e1s, potenci\u00e1lisan ismeretlen vegy\u00fcletek a tirozin\u00e1z tartalom n\u00f6vel\u00e9s\u00e9vel gyakorolhatnak ter\u00e1pi\u00e1s hat\u00e1st a vitilig\u00f3ra. Vannak azonban irodalmi jelent\u00e9sek arr\u00f3l is, hogy a PSO hat az A375 hum\u00e1n melan\u00f3ma sejtvonalra, g\u00e1tolva a tirozin\u00e1z prom\u00f3ter aktivit\u00e1s\u00e1t; A Tyr, Tyrp1 \u00e9s Tyrp2 mRNS expresszi\u00f3s szintj\u00e9nek lefel\u00e9 szab\u00e1lyoz\u00e1sa \u00e9s a melanin termel\u00e9s g\u00e1tl\u00e1sa, ellent\u00e9tben a k\u00eds\u00e9rlet eredm\u00e9nyeivel, a k\u00fcl\u00f6nb\u00f6z\u0151 kutat\u00e1si alanyoknak vagy a melanin termel\u00e9s PSO \u00e1ltali k\u00e9tir\u00e1ny\u00fa szab\u00e1lyoz\u00e1s\u00e1nak k\u00f6sz\u00f6nhet\u0151 a k\u00fcl\u00f6nb\u00f6z\u0151 koncentr\u00e1ci\u00f3tartom\u00e1nyokban. Tov\u00e1bbi kutat\u00e1sokra van sz\u00fcks\u00e9g a szigor\u00fabb \u00e9s pontosabb k\u00f6vetkeztet\u00e9sek levon\u00e1s\u00e1hoz. A vitiligo psoral\u00e9nnel t\u00f6rt\u00e9n\u0151 kezel\u00e9s\u00e9ben a PSO \u00e9s az IPSO a f\u0151 hat\u00f3anyagok, amelyek min\u0151s\u00e9gellen\u0151rz\u00e9si indik\u00e1tork\u00e9nt haszn\u00e1lhat\u00f3k e gy\u00f3gyszer vagy az e hagyom\u00e1nyos k\u00ednai gy\u00f3gyszert tartalmaz\u00f3 k\u00e9sz\u00edtm\u00e9nyek eset\u00e9ben.<\/p>","protected":false},"excerpt":{"rendered":"<p>Chemical composition analysis and preliminary exploration of the mechanism of promoting melanin production in the alcohol extract of Fructus Psorale by UPLC-Q-TOF-MS Vitiligo is a common clinical localized depigmentation skin disease, with a prevalence rate of about 0.5%~2% worldwide. It is easy to diagnose and difficult to treat, with a high incidence rate and a [&hellip;]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.0 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Chemical composition analysis and preliminary exploration of the mechanism of promoting melanin production in the alcohol extract of Fructus Psorale by UPLC-Q-TOF-MS - China Chemical Manufacturer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangextracts.com\/hu\/uplc-q-tof-ms-fructus-psorale-extrakcioja\/\" \/>\n<meta property=\"og:locale\" content=\"hu_HU\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Chemical composition analysis and preliminary exploration of the mechanism of promoting melanin production in the alcohol extract of Fructus Psorale by UPLC-Q-TOF-MS - China Chemical Manufacturer\" \/>\n<meta property=\"og:description\" content=\"Chemical composition analysis and preliminary exploration of the mechanism of promoting melanin production in the alcohol extract of Fructus Psorale by UPLC-Q-TOF-MS Vitiligo is a common clinical localized depigmentation skin disease, with a prevalence rate of about 0.5%~2% worldwide. 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